Restriction map for
pFN21AB9417
(Circular) (Six-base) MAPSORT of: pFN21AB9417.seq Check: 9356 from: 1 to: 7360
pFN21AB9417 7360 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5170 278
Size: 53 83 186 936 3634 2468
Fragments arranged by size:
3634 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4152 4703 1937
Size: 2215 551 4594
Fragments arranged by size:
4594 2215 551
Acc65I G'GTAC_C
Cuts at: 3089 3102 3468 4681 3089
Size: 13 366 1213 5768
Fragments arranged by size:
5768 1213 366 13
AclI AA'CG_TT
Cuts at: 1563 5487 5860 1563
Size: 3924 373 3063
Fragments arranged by size:
3924 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2121 3525 3563 4301 4418 5423 6807 7075
Size: 489 1404 38 738 117 1005 1384 268
Cuts at: 7075 1632
Size: 1917
Fragments arranged by size:
1917 1404 1384 1005 738 489 268 117
38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7163
AflIII A'CryG_T
Cuts at: 1203 4026 6259 7236 1203
Size: 2823 2233 977 1327
Fragments arranged by size:
2823 2233 1327 977
AgeI A'CCGG_T
Cuts at: 6191 6191
Size: 7360
AhdI GACnn_n'nnGTC
Cuts at: 4574 6089 4574
Size: 1515 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7360
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2588 2620 2588
Size: 32 7328
AlwNI CAG_nnn'CTG
Cuts at: 2230 3824 4008 5103 6675 2230
Size: 1594 184 1095 1572 2915
Fragments arranged by size:
2915 1594 1572 1095 184
ApaI G_GGCC'C
Cuts at: 2954 3011 2954
Size: 57 7303
ApaLI G'TGCA_C
Cuts at: 5417 6573 5417
Size: 1156 6204
ApoI r'AATT_y
Cuts at: 1493 3749 4669 4895 5047 7156 1493
Size: 2256 920 226 152 2109 1697
Fragments arranged by size:
2256 2109 1697 920 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5914 160
Size: 872 4882 1606
Fragments arranged by size:
4882 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7360
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2198 3072 3887 4685 1870
Size: 73 202 53 874 815 798 4545
Fragments arranged by size:
4545 874 815 798 202 73 53
BamHI G'GATC_C
Cuts at: 4690 5057 4690
Size: 367 6993
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2141 2159 2260 3076
Size: 325 217 627 229 125 18 101 816
Cuts at: 3076 3089 3102 3282 3468 4655 4681 6136 618
Size: 13 13 180 186 1187 26 1455 1842
Fragments arranged by size:
1842 1455 1187 816 627 325 229 217
186 180 125 101 26 18 13 13
BanII G_rGCy'C
Cuts at: 1391 2196 2238 2439 2508 2850 2954 3011 3072
Size: 805 42 201 69 342 104 57 61
Cuts at: 3072 4056 4628 4679 1391
Size: 984 572 51 4072
Fragments arranged by size:
4072 984 805 572 342 201 104 69
61 57 51 42
BbeI G_GCGC'C
Cuts at: 3080 3286 3080
Size: 206 7154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2448 3602 3683 928
Size: 971 549 1154 81 4605
Fragments arranged by size:
4605 1154 971 549 81
BbvCI CC'TCA_GC
Cuts at: 4551 4551
Size: 7360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5551 5585 1903
Size: 34 3614 34 3678
Fragments arranged by size:
3678 3614 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5252 6462 5252
Size: 1210 6150
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7360
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2754 5971 136
Size: 107 122 71 631 605 1082 3217 1525
Fragments arranged by size:
3217 1525 1082 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4065 7355 4065
Size: 3290 4070
BlpI GC'TnA_GC
Cuts at: 4608 4777 4608
Size: 169 7191
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2093 2146 2162 2265 2874 2954 3003
Size: 231 72 53 16 103 609 80 49
Cuts at: 3003 3011 5421 6577 1790
Size: 8 2410 1156 2573
Fragments arranged by size:
2573 2410 1156 609 231 103 80 72
53 49 16 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3257 3281 5091
Size: 416 53 83 399 24 1840 24 1810
Cuts at: 5091 6049 442
Size: 958 1753
Fragments arranged by size:
1840 1810 1753 958 416 399 83 53
24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2252 2429 2450 2510 2538 2727 2747
Size: 923 90 177 21 60 28 189 20
Cuts at: 2747 3197 3274 3293 3734 4500 6020 1239
Size: 450 77 19 441 766 1520 2579
Fragments arranged by size:
2579 1520 923 766 450 441 189 177
90 77 60 28 21 20 19
Bpu10I CC'TnA_GC
Cuts at: 2477 2490 2750 3158 4551 2477
Size: 13 260 408 1393 5286
Fragments arranged by size:
5286 1393 408 260 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3647 5485 6350 6648 3647
Size: 1838 865 298 4359
Fragments arranged by size:
4359 1838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2710 3404 3849 6023 882
Size: 617 1211 694 445 2174 2219
Fragments arranged by size:
2219 2174 1211 694 617 445
BsaAI yAC'GTr
Cuts at: 493 1204 5151 493
Size: 711 3947 2702
Fragments arranged by size:
3947 2702 711
BsaBI GATnn'nnATC
Cuts at: 2813 3692 7354 2813
Size: 879 3662 2819
Fragments arranged by size:
3662 2819 879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2987 3077 3283 5167
Size: 53 83 186 936 1454 90 206 1884
Cuts at: 5167 5549 6184 275
Size: 382 635 1451
Fragments arranged by size:
1884 1454 1451 936 635 382 206 186
90 83 53
BsaWI w'CCGG_w
Cuts at: 5793 6191 6465 6612 7023 5793
Size: 398 274 147 411 6130
Fragments arranged by size:
6130 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2508 2538 2690 2720 3336
Size: 30 165 30 1105 30 152 30 616
Cuts at: 3336 3366 1178
Size: 30 5172
Fragments arranged by size:
5172 1105 616 165 152 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 2858 3863 4506 1186
Size: 1261 411 1005 643 4040
Fragments arranged by size:
4040 1261 1005 643 411
BseYI C'CCAG_C
Cuts at: 2067 2388 2568 2679 3239 3817 4332 4509 4630
Size: 321 180 111 560 578 515 177 121
Cuts at: 4630 6563 7059 2067
Size: 1933 496 2368
Fragments arranged by size:
2368 1933 578 560 515 496 321 180
177 121 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2761 3682 1163
Size: 573 1025 921 4841
Fragments arranged by size:
4841 1025 921 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4810 5571 6599 1540
Size: 275 186 2809 761 1028 2301
Fragments arranged by size:
2809 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2676 5421 5506 6577 2238
Size: 438 2745 85 1071 3021
Fragments arranged by size:
3021 2745 1071 438 85
BsmI GAATG_Cn'
Cuts at: 4871 4964 4871
Size: 93 7267
BsmBI CGTCTCn'nnnn_
Cuts at: 2978 2978
Size: 7360
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2093 2146 2162 2196 2238 2265
Size: 399 231 72 53 16 34 42 27
Cuts at: 2265 2439 2508 2676 2850 2874 2954 3003 3011
Size: 174 69 168 174 24 80 49 8
Cuts at: 3011 3072 4056 4628 4679 5421 5506 6577 1391
Size: 61 984 572 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 984 742 572 399 231 174
174 168 85 80 72 69 61 53
51 49 42 34 27 24 16 8
BspEI T'CCGG_A
Cuts at: 7023 7023
Size: 7360
BspHI T'CATG_A
Cuts at: 3768 3843 5249 6979 3768
Size: 75 1406 1730 4149
Fragments arranged by size:
4149 1730 1406 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3915 4715 844
Size: 3071 800 3489
Fragments arranged by size:
3489 3071 800
BsrBI CCG'CTC
Cuts at: 1394 1823 2754 4607 5247 1394
Size: 429 931 1853 640 3507
Fragments arranged by size:
3507 1853 931 640 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5855 6029 65
Size: 1183 4607 174 1396
Fragments arranged by size:
4607 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3105 3396 3573 4353 6004 6191 1700
Size: 110 1295 291 177 780 1651 187 2869
Fragments arranged by size:
2869 1651 1295 780 291 187 177 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7360
BssSI C'ACGA_G
Cuts at: 3316 3700 5420 6432 3316
Size: 384 1720 1012 4244
Fragments arranged by size:
4244 1720 1012 384
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2791 794
Size: 1997 5363
BstBI TT'CG_AA
Cuts at: 6164 6164
Size: 7360
BstEII G'GTnAC_C
Cuts at: 1184 2641 3169 1184
Size: 1457 528 5375
Fragments arranged by size:
5375 1457 528
BstXI CCAn_nnnn'nTGG
Cuts at: 2725 3955 4119 4565 2725
Size: 1230 164 446 5520
Fragments arranged by size:
5520 1230 446 164
BstYI r'GATC_y
Cuts at: 1972 2226 4065 4690 5057 5442 5459 6911 6997
Size: 254 1839 625 367 385 17 1452 86
Cuts at: 6997 7009 7355 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1839 1452 625 385 367 346 254
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4521 4521
Size: 7360
BtgI C'CryG_G
Cuts at: 513 1065 2004 2508 2547 2718 513
Size: 552 939 504 39 171 5155
Fragments arranged by size:
5155 939 552 504 171 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3928 4182 487
Size: 683 56 2702 254 3665
Fragments arranged by size:
3665 2702 683 254 56
BtsI GCAGTG_nn'
Cuts at: 2773 3231 4542 4876 5670 5690 2773
Size: 458 1311 334 794 20 4443
Fragments arranged by size:
4443 1311 794 458 334 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7325
DraI TTT'AAA
Cuts at: 4664 4664
Size: 7360
DraIII CAC_nnn'GTG
Cuts at: 3583 3583
Size: 7360
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2383 6367 809
Size: 507 1067 3984 1802
Fragments arranged by size:
3984 1802 1067 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2454 2544 3354
Size: 54 1425 50 275 18 624 90 810
Cuts at: 3354 3999 4355 4807 5696 7325 8
Size: 645 356 452 889 1629 43
Fragments arranged by size:
1629 1425 889 810 645 624 452 356
275 90 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4807 1537
Size: 275 2995 4090
Fragments arranged by size:
4090 2995 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3389 3531 5290 1353
Size: 2036 142 1759 3423
Fragments arranged by size:
3423 2036 1759 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4058 5947 6321 6467 387
Size: 1064 138 2469 1889 374 146 1280
Fragments arranged by size:
2469 1889 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2236 2236
Size: 7360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2162 2252 2429 2450 2510 2538
Size: 393 489 41 90 177 21 60 28
Cuts at: 2538 2727 2747 3197 3274 3293 3525 3563 3734
Size: 189 20 450 77 19 232 38 171
Cuts at: 3734 4301 4418 4500 5423 6020 6807 7075 1239
Size: 567 117 82 923 597 787 268 1524
Fragments arranged by size:
1524 923 787 597 567 489 450 393
268 232 189 177 171 117 90 82
77 60 41 38 28 21 20 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2270 2270
Size: 7360
EcoO109I rG'GnC_Cy
Cuts at: 2035 2495 2950 2951 3008 3991 2035
Size: 460 455 1 57 983 5404
Fragments arranged by size:
5404 983 460 455 57 1
EcoRI G'AATT_C
Cuts at: 4669 5047 4669
Size: 378 6982
FspI TGC'GCA
Cuts at: 3479 5866 3479
Size: 2387 4973
HaeII r_GCGC'y
Cuts at: 1678 2060 3080 3286 6507 1678
Size: 382 1020 206 3221 2531
Fragments arranged by size:
3221 2531 1020 382 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4013 4045 4160 4192 4560
Size: 32 135 32 2441 32 115 32 368
Cuts at: 4560 4592 4610 4642 6001 6033 6075 6107 1373
Size: 32 18 32 1359 32 42 32 2626
Fragments arranged by size:
2626 2441 1359 368 135 115 42 32
32 32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 4444 4704 4950 669
Size: 988 281 2506 260 246 3079
Fragments arranged by size:
3079 2506 988 281 260 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7360
HpaI GTT'AAC
Cuts at: 4950 4950
Size: 7360
KasI G'GCGC_C
Cuts at: 3076 3282 3076
Size: 206 7154
KpnI G_GTAC'C
Cuts at: 3093 3106 3472 4685 3093
Size: 13 366 1213 5768
Fragments arranged by size:
5768 1213 366 13
MfeI C'AATT_G
Cuts at: 4959 4959
Size: 7360
MluI A'CGCG_T
Cuts at: 7236 7236
Size: 7360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 3476 6474 6658 1305
Size: 1053 1118 2998 184 2007
Fragments arranged by size:
2998 2007 1118 1053 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2546 10
Size: 54 1425 343 714 4824
Fragments arranged by size:
4824 1425 714 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 2927 3917 4031 5318 5677 5836
Size: 648 1503 258 990 114 1287 359 159
Cuts at: 5836 518
Size: 2042
Fragments arranged by size:
2042 1503 1287 990 648 359 258 159
114
MspA1I CmG'CkG
Cuts at: 1606 2041 3359 4776 5453 6601 6846 1606
Size: 435 1318 1417 677 1148 245 2120
Fragments arranged by size:
2120 1417 1318 1148 677 435 245
NarI GG'CG_CC
Cuts at: 3077 3283 3077
Size: 206 7154
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5869
Fragments arranged by size:
5869 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7360
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7360
NotI GC'GGCC_GC
Cuts at: 4807 4807
Size: 7360
NspI r_CATG'y
Cuts at: 1132 1229 2585 2937 4015 4030 4718 6263 1132
Size: 97 1356 352 1078 15 688 1545 2229
Fragments arranged by size:
2229 1545 1356 1078 688 352 97 15
PasI CC'CwG_GG
Cuts at: 1348 2085 2126 2266 3004 3589 4640 1348
Size: 737 41 140 738 585 1051 4068
Fragments arranged by size:
4068 1051 738 737 585 140 41
PciI A'CATG_T
Cuts at: 4026 6259 4026
Size: 2233 5127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2356 1108
Size: 902 346 6112
Fragments arranged by size:
6112 902 346
PfoI T'CCnGG_A
Cuts at: 2229 2427 2526 2960 2229
Size: 198 99 434 6629
Fragments arranged by size:
6629 434 198 99
PmeI GTTT'AAAC
Cuts at: 4664 4664
Size: 7360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5429 5461 6942 6974 5429
Size: 32 1481 32 5815
Fragments arranged by size:
5815 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2035 2495 3991 2035
Size: 460 1496 5404
Fragments arranged by size:
5404 1496 460
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7360
PsiI TTA'TAA
Cuts at: 4930 4930
Size: 7360
PspOMI G'GGCC_C
Cuts at: 2950 3007 2950
Size: 57 7303
PstI C_TGCA'G
Cuts at: 830 2328 2787 3087 3186 4489 4712 830
Size: 1498 459 300 99 1303 223 3478
Fragments arranged by size:
3478 1498 1303 459 300 223 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7360
SacI G_AGCT'C
Cuts at: 2238 2238
Size: 7360
SalI G'TCGA_C
Cuts at: 1936 4702 1936
Size: 2766 4594
SanDI GG'GwC_CC
Cuts at: 2035 2495 2035
Size: 460 6900
SapI GCTCTTCn'nnn_
Cuts at: 3531 3531
Size: 7360
SbfI CC_TGCA'GG
Cuts at: 2787 3087 4712 2787
Size: 300 1625 5435
Fragments arranged by size:
5435 1625 300
ScaI AGT'ACT
Cuts at: 5608 7320 5608
Size: 1712 5648
SexAI A'CCwGG_T
Cuts at: 2700 2700
Size: 7360
SfcI C'TryA_G
Cuts at: 826 1045 2324 2783 3083 3182 3487 4485 4531
Size: 219 1279 459 300 99 305 998 46
Cuts at: 4531 4708 5843 6524 6715 826
Size: 177 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 998 681 459 305 300
219 191 177 99 46
SfoI GGC'GCC
Cuts at: 3078 3284 3078
Size: 206 7154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7360
SgrAI Cr'CCGG_yG
Cuts at: 1700 3396 1700
Size: 1696 5664
SmaI CCC'GGG
Cuts at: 1872 2147 3074 4687 1872
Size: 275 927 1613 4545
Fragments arranged by size:
4545 1613 927 275
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3662 5464 6365 6627 820
Size: 197 926 1719 1802 901 262 1553
Fragments arranged by size:
1802 1719 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5151 493
Size: 4658 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7360
SphI G_CATG'C
Cuts at: 1132 2937 4015 4718 1132
Size: 1805 1078 703 3774
Fragments arranged by size:
3774 1805 1078 703
SspI AAT'ATT
Cuts at: 5 52 5284 5
Size: 47 5232 2081
Fragments arranged by size:
5232 2081 47
StuI AGG'CCT
Cuts at: 1517 2748 3050 3278 4403 1517
Size: 1231 302 228 1125 4474
Fragments arranged by size:
4474 1231 1125 302 228
StyI C'CwwG_G
Cuts at: 513 1065 2004 2634 4799 513
Size: 552 939 630 2165 3074
Fragments arranged by size:
3074 2165 939 630 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 2264 5387 5404 5557 5742 6187
Size: 1255 678 6 3123 17 153 185 445
Cuts at: 6187 325
Size: 1498
Fragments arranged by size:
3123 1498 1255 678 445 185 153 17
6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3334 5606 7318
Size: 274 80 33 51 1443 1357 2272 1712
Cuts at: 7318 96
Size: 138
Fragments arranged by size:
2272 1712 1443 1357 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2690 2722 3336 3368 7320 7352 2690
Size: 32 614 32 3952 32 2698
Fragments arranged by size:
3952 2698 614 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4148 1316
Size: 2832 4528
XbaI T'CTAG_A
Cuts at: 4696 4696
Size: 7360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3664 3664
Size: 7360
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7360
XmaI C'CCGG_G
Cuts at: 1870 2145 3072 4685 1870
Size: 275 927 1613 4545
Fragments arranged by size:
4545 1613 927 275
XmnI GAAnn'nnTTC
Cuts at: 5489 5489
Size: 7360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5168 276
Size: 53 83 186 936 3634 2468
Fragments arranged by size:
3634 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII
SfiI SrfI SwaI