Restriction map for
pFN21AB9409
(Circular) (Six-base) MAPSORT of: pFN21AB9409.seq Check: 5488 from: 1 to: 5572
pFN21AB9409 5572 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2915 1937
Size: 978 4594
Acc65I G'GTAC_C
Cuts at: 2893 2893
Size: 5572
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3635 5019 5287 1632
Size: 2003 1384 268 1917
Fragments arranged by size:
2003 1917 1384 268
AfeI AGC'GCT
Cuts at: 2111 2111
Size: 5572
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 4471 5448 1203
Size: 3268 977 1327
Fragments arranged by size:
3268 1327 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 4301 4301
Size: 5572
AleI CACnn'nnGTG
Cuts at: 1166 2720 1166
Size: 1554 4018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2063 1184
Size: 879 4693
AlwNI CAG_nnn'CTG
Cuts at: 2092 2410 3315 4887 2092
Size: 318 905 1572 2777
Fragments arranged by size:
2777 1572 905 318
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2881 3107 3259 5368 1493
Size: 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2897 1870
Size: 73 954 4545
Fragments arranged by size:
4545 954 73
BamHI G'GATC_C
Cuts at: 2807 2902 3269 2807
Size: 95 367 5110
Fragments arranged by size:
5110 367 95
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2893 4348 618
Size: 325 217 627 1106 1455 1842
Fragments arranged by size:
1842 1455 1106 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2646 2669 2891 1391
Size: 1255 23 222 4072
Fragments arranged by size:
4072 1255 222 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 928
Size: 971 325 4276
Fragments arranged by size:
4276 971 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 1903
Size: 34 1826 34 3678
Fragments arranged by size:
3678 1826 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3464 4674 3464
Size: 1210 4362
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4183 136
Size: 107 122 71 631 605 2511 1525
Fragments arranged by size:
2511 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2989 2989
Size: 5572
Bme1580I G_kGCm'C
Cuts at: 1790 3633 4789 1790
Size: 1843 1156 2573
Fragments arranged by size:
2573 1843 1156
BmgBI CAC'GTC
Cuts at: 1318 2722 2854 1318
Size: 1404 132 4036
Fragments arranged by size:
4036 1404 132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 4261 442
Size: 416 53 83 399 24 1886 958 1753
Fragments arranged by size:
1886 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2435 2630 4232 1239
Size: 977 219 195 1602 2579
Fragments arranged by size:
2579 1602 977 219 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3697 4562 4860 3697
Size: 865 298 4409
Fragments arranged by size:
4409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2339 4235 882
Size: 617 840 1896 2219
Fragments arranged by size:
2219 1896 840 617
BsaAI yAC'GTr
Cuts at: 493 1204 3363 493
Size: 711 2159 2702
Fragments arranged by size:
2702 2159 711
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3379 3761 4396 275
Size: 53 83 186 936 1846 382 635 1451
Fragments arranged by size:
1846 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4005 4403 4677 4824 5235 4005
Size: 398 274 147 411 4342
Fragments arranged by size:
4342 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2357 2387 2510
Size: 30 165 30 597 30 327 30 123
Cuts at: 2510 2540 1178
Size: 30 4210
Fragments arranged by size:
4210 597 327 165 123 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2460 2756 1186
Size: 934 340 296 4002
Fragments arranged by size:
4002 934 340 296
BseYI C'CCAG_C
Cuts at: 2270 2434 2508 4775 5271 2270
Size: 164 74 2267 496 2571
Fragments arranged by size:
2571 2267 496 164 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 1163
Size: 573 429 4570
Fragments arranged by size:
4570 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2077 3022 3783 4811 1540
Size: 275 186 76 945 761 1028 2301
Fragments arranged by size:
2301 1028 945 761 275 186 76
BsiHKAI G_wGCw'C
Cuts at: 2129 2490 2669 2766 3633 3718 4789 2129
Size: 361 179 97 867 85 1071 2912
Fragments arranged by size:
2912 1071 867 361 179 97 85
BsmI GAATG_Cn'
Cuts at: 3083 3176 3083
Size: 93 5479
BsmBI CGTCTCn'nnnn_
Cuts at: 2064 2064
Size: 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2490 2646 2669 2766 2891 3633
Size: 399 339 361 156 23 97 125 742
Cuts at: 3633 3718 4789 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 339 156 125
97 85 23
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 2473 3461 5191 2473
Size: 988 1730 2854
Fragments arranged by size:
2854 1730 988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2100 2927 844
Size: 1226 30 827 3489
Fragments arranged by size:
3489 1226 827 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3459 1394
Size: 429 1636 3507
Fragments arranged by size:
3507 1636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2556 4067 4241 65
Size: 1183 1308 1511 174 1396
Fragments arranged by size:
1511 1396 1308 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4216 4403 1700
Size: 110 2406 187 2869
Fragments arranged by size:
2869 2406 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5572
BssSI C'ACGA_G
Cuts at: 2021 3632 4644 2021
Size: 1611 1012 2949
Fragments arranged by size:
2949 1611 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2092 2494 794
Size: 1298 402 3872
Fragments arranged by size:
3872 1298 402
BstBI TT'CG_AA
Cuts at: 4376 4376
Size: 5572
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5572
BstYI r'GATC_y
Cuts at: 1972 2807 2902 3269 3654 3671 5123 5209 5221
Size: 835 95 367 385 17 1452 86 12
Cuts at: 5221 5567 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 835 385 367 346 95 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2403 2403
Size: 5572
BtgI C'CryG_G
Cuts at: 513 1065 2004 2361 513
Size: 552 939 357 3724
Fragments arranged by size:
3724 939 552 357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4833
Fragments arranged by size:
4833 683 56
BtsI GCAGTG_nn'
Cuts at: 2129 3088 3882 3902 2129
Size: 959 794 20 3799
Fragments arranged by size:
3799 959 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2876 2876
Size: 5572
DraIII CAC_nnn'GTG
Cuts at: 2026 2026
Size: 5572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2078 2287 4579 809
Size: 507 762 209 2292 1802
Fragments arranged by size:
2292 1802 762 507 209
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2241 3019
Size: 54 1425 50 275 18 351 60 778
Cuts at: 3019 3908 5537 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 778 351 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3019 1537
Size: 275 1207 4090
Fragments arranged by size:
4090 1207 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 3502 1353
Size: 867 1282 3423
Fragments arranged by size:
3423 1282 867
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4159 4533 4679 387
Size: 1064 138 2570 374 146 1280
Fragments arranged by size:
2570 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2667 2667
Size: 5572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2435 2630 3635 4232 5019 5287
Size: 393 584 219 195 1005 597 787 268
Cuts at: 5287 1239
Size: 1524
Fragments arranged by size:
1524 1005 787 597 584 393 268 219
195
EcoO109I rG'GnC_Cy
Cuts at: 2275 2275
Size: 5572
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
EcoRV GAT'ATC
Cuts at: 2863 2863
Size: 5572
FspI TGC'GCA
Cuts at: 2171 2498 4078 2171
Size: 327 1580 3665
Fragments arranged by size:
3665 1580 327
HaeII r_GCGC'y
Cuts at: 1678 2113 4719 1678
Size: 435 2606 2531
Fragments arranged by size:
2606 2531 435
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2510 2542 2619 2651 4213
Size: 32 135 32 938 32 77 32 1562
Cuts at: 4213 4245 4287 4319 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1562 938 135 77 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2916 3162 669
Size: 988 281 978 246 3079
Fragments arranged by size:
3079 988 978 281 246
HindIII A'AGCT_T
Cuts at: 748 2736 748
Size: 1988 3584
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KpnI G_GTAC'C
Cuts at: 2897 2897
Size: 5572
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2182 2796 4686 4870 1305
Size: 877 614 1890 184 2007
Fragments arranged by size:
2007 1890 877 614 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2183 2243 10
Size: 54 1425 343 351 60 3339
Fragments arranged by size:
3339 1425 351 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2720 3530 3889 4048 518
Size: 648 1023 531 810 359 159 2042
Fragments arranged by size:
2042 1023 810 648 531 359 159
MspA1I CmG'CkG
Cuts at: 1606 2356 2363 2462 2515 2988 3665 4813 5058
Size: 750 7 99 53 473 677 1148 245
Cuts at: 5058 1606
Size: 2120
Fragments arranged by size:
2120 1148 750 677 473 245 99 53
7
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4081
Fragments arranged by size:
4081 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5572
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2170 2323 2546 2930 4475 1132
Size: 97 941 153 223 384 1545 2229
Fragments arranged by size:
2229 1545 941 384 223 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5572
PciI A'CATG_T
Cuts at: 4471 4471
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2026 1108
Size: 918 4654
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2275
Size: 5572
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PstI C_TGCA'G
Cuts at: 830 2088 2781 2924 830
Size: 1258 693 143 3478
Fragments arranged by size:
3478 1258 693 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
PvuII CAG'CTG
Cuts at: 2462 2462
Size: 5572
RsrII CG'GwC_CG
Cuts at: 2035 2035
Size: 5572
SacI G_AGCT'C
Cuts at: 2669 2669
Size: 5572
SacII CC_GC'GG
Cuts at: 2364 2364
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SanDI GG'GwC_CC
Cuts at: 2275 2275
Size: 5572
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 3820 5532 3820
Size: 1712 3860
SexAI A'CCwGG_T
Cuts at: 2868 2868
Size: 5572
SfcI C'TryA_G
Cuts at: 826 1045 2084 2777 2847 2920 4055 4736 4927
Size: 219 1039 693 70 73 1135 681 191
Cuts at: 4927 826
Size: 1471
Fragments arranged by size:
1471 1135 1039 693 681 219 191 73
70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2899 1872
Size: 1027 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3676 4577 4839 820
Size: 197 926 1733 901 262 1553
Fragments arranged by size:
1733 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2546 2930 1132
Size: 1414 384 3774
Fragments arranged by size:
3774 1414 384
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 2004 3011 513
Size: 552 939 1007 3074
Fragments arranged by size:
3074 1007 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 3599 3616 3769 3954 4399 325
Size: 1255 717 1302 17 153 185 445 1498
Fragments arranged by size:
1498 1302 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3818 5530 96
Size: 274 80 33 51 1443 1841 1712 138
Fragments arranged by size:
1841 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2201 2233 2357 2389 5532 5564 2000
Size: 32 169 32 124 32 3143 32 2008
Fragments arranged by size:
3143 2008 169 124 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2033 2078 1316
Size: 717 45 4810
Fragments arranged by size:
4810 717 45
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2251 2719 2191
Size: 60 468 5044
Fragments arranged by size:
5044 468 60
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2897 1870
Size: 1027 4545
XmnI GAAnn'nnTTC
Cuts at: 3701 3701
Size: 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI SapI SfiI
SfoI SrfI SwaI