Restriction map for
pFN21AB9407
(Circular) (Six-base) MAPSORT of: pFN21AB9407.seq Check: 1152 from: 1 to: 8479
pFN21AB9407 8479 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4333 4450 4333
Size: 117 8362
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6289 278
Size: 53 83 186 936 4753 2468
Fragments arranged by size:
4753 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 3141 5822 1937
Size: 717 487 2681 4594
Fragments arranged by size:
4594 2681 717 487
Acc65I G'GTAC_C
Cuts at: 5800 5800
Size: 8479
AclI AA'CG_TT
Cuts at: 1563 6606 6979 1563
Size: 5043 373 3063
Fragments arranged by size:
5043 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 3353 5720 6542 7926 8194 1632
Size: 986 735 2367 822 1384 268 1917
Fragments arranged by size:
2367 1917 1384 986 822 735 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8282
AflIII A'CryG_T
Cuts at: 1203 7378 8355 1203
Size: 6175 977 1327
Fragments arranged by size:
6175 1327 977
AgeI A'CCGG_T
Cuts at: 7310 7310
Size: 8479
AhdI GACnn_n'nnGTC
Cuts at: 7208 7208
Size: 8479
AleI CACnn'nnGTG
Cuts at: 1166 4304 4988 1166
Size: 3138 684 4657
Fragments arranged by size:
4657 3138 684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4322 4758 5006 1184
Size: 3138 436 248 4657
Fragments arranged by size:
4657 3138 436 248
AlwNI CAG_nnn'CTG
Cuts at: 6222 7794 6222
Size: 1572 6907
ApaI G_GGCC'C
Cuts at: 2189 4399 2189
Size: 2210 6269
ApaLI G'TGCA_C
Cuts at: 6536 7692 6536
Size: 1156 7323
ApoI r'AATT_y
Cuts at: 1493 4492 5604 5788 6014 6166 8275 1493
Size: 2999 1112 184 226 152 2109 1697
Fragments arranged by size:
2999 2109 1697 1112 226 184 152
AseI AT'TA_AT
Cuts at: 160 1032 4673 7033 160
Size: 872 3641 2360 1606
Fragments arranged by size:
3641 2360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8479
AvaI C'yCGr_G
Cuts at: 1870 1943 2188 2513 4313 4921 5649 5804 1870
Size: 73 245 325 1800 608 728 155 4545
Fragments arranged by size:
4545 1800 728 608 325 245 155 73
BamHI G'GATC_C
Cuts at: 5809 6176 5809
Size: 367 8112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 3003 3644 4038 4795
Size: 325 217 627 756 460 641 394 757
Cuts at: 4795 4816 5800 7255 618
Size: 21 984 1455 1842
Fragments arranged by size:
1842 1455 984 757 756 641 627 460
394 325 217 21
BanII G_rGCy'C
Cuts at: 1391 2189 2391 4202 4399 4922 5147 5798 1391
Size: 798 202 1811 197 523 225 651 4072
Fragments arranged by size:
4072 1811 798 651 523 225 202 197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3120 3164 928
Size: 971 1221 44 6243
Fragments arranged by size:
6243 1221 971 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6670 6704 1903
Size: 34 4733 34 3678
Fragments arranged by size:
4733 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6371 7581 6371
Size: 1210 7269
BclI T'GATC_A
Cuts at: 1548 2380 2607 1548
Size: 832 227 7420
Fragments arranged by size:
7420 832 227
BfrBI ATG'CAT
Cuts at: 5099 5099
Size: 8479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7090 136
Size: 107 122 71 631 605 5418 1525
Fragments arranged by size:
5418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3621 8474 3621
Size: 4853 3626
BlpI GC'TnA_GC
Cuts at: 5551 5896 5551
Size: 345 8134
Bme1580I G_kGCm'C
Cuts at: 1790 2189 4399 4800 4821 6540 7696 1790
Size: 399 2210 401 21 1719 1156 2573
Fragments arranged by size:
2573 2210 1719 1156 401 399 21
BmgBI CAC'GTC
Cuts at: 1318 2260 3856 4759 5005 1318
Size: 942 1596 903 246 4792
Fragments arranged by size:
4792 1596 942 903 246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2932 4393 4637
Size: 416 53 83 399 24 1515 1461 244
Cuts at: 4637 6210 7168 442
Size: 1573 958 1753
Fragments arranged by size:
1753 1573 1515 1461 958 416 399 244
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3792 1056
Size: 2736 5743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2544 4837 2544
Size: 2293 6186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2375 3482 4326 4564 4683 5760 7139
Size: 998 138 1107 844 238 119 1077 1379
Cuts at: 7139 1239
Size: 2579
Fragments arranged by size:
2579 1379 1107 1077 998 844 238 138
119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 3594 6604 7469 7767 3227
Size: 367 3010 865 298 3939
Fragments arranged by size:
3939 3010 865 367 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2379 3764 5172 5618 7142 882
Size: 617 880 1385 1408 446 1524 2219
Fragments arranged by size:
2219 1524 1408 1385 880 617 446
BsaAI yAC'GTr
Cuts at: 493 1204 6270 493
Size: 711 5066 2702
Fragments arranged by size:
5066 2702 711
BsaBI GATnn'nnATC
Cuts at: 8473 8473
Size: 8479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4691 6286 6668 7303
Size: 53 83 186 936 3158 1595 382 635
Cuts at: 7303 275
Size: 1451
Fragments arranged by size:
3158 1595 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5181 6912 7310 7584 7731 8142 5181
Size: 1731 398 274 147 411 5518
Fragments arranged by size:
5518 1731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 2431 2461 4293
Size: 30 165 30 711 30 287 30 1832
Cuts at: 4293 4323 1178
Size: 30 5334
Fragments arranged by size:
5334 1832 711 287 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2735 2738 2798 3080 4188 4822 5496 5526
Size: 1549 3 60 282 1108 634 674 30
Cuts at: 5526 1186
Size: 4139
Fragments arranged by size:
4139 1549 1108 674 634 282 60 30
3
BseYI C'CCAG_C
Cuts at: 2086 2101 2194 2263 3847 7682 8178 2086
Size: 15 93 69 1584 3835 496 2387
Fragments arranged by size:
3835 2387 1584 496 93 69 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 4112 4259 5012 1163
Size: 573 507 1869 147 753 4630
Fragments arranged by size:
4630 1869 753 573 507 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2154 4651 5929 6690 7718 1540
Size: 275 186 153 2497 1278 761 1028 2301
Fragments arranged by size:
2497 2301 1278 1028 761 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 2118 2391 2790 4311 4922 6540 6625 7696 2118
Size: 273 399 1521 611 1618 85 1071 2901
Fragments arranged by size:
2901 1618 1521 1071 611 399 273 85
BsiWI C'GTAC_G
Cuts at: 2130 2130
Size: 8479
BsmI GAATG_Cn'
Cuts at: 2060 5308 5644 5990 6083 2060
Size: 3248 336 346 93 4456
Fragments arranged by size:
4456 3248 346 336 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5703 5703
Size: 8479
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2189 2391 2790 4202 4311 4399
Size: 399 328 71 202 399 1412 109 88
Cuts at: 4399 4800 4821 4922 5147 5798 6540 6625 7696
Size: 401 21 101 225 651 742 85 1071
Cuts at: 7696 1391
Size: 2174
Fragments arranged by size:
2174 1412 1071 742 651 401 399 399
328 225 202 109 101 88 85 71
21
BspEI T'CCGG_A
Cuts at: 8142 8142
Size: 8479
BspHI T'CATG_A
Cuts at: 2383 6368 8098 2383
Size: 3985 1730 2764
Fragments arranged by size:
3985 2764 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 4333 4450 5834 844
Size: 1502 1987 117 1384 3489
Fragments arranged by size:
3489 1987 1502 1384 117
BsrBI CCG'CTC
Cuts at: 1394 1823 2141 6366 1394
Size: 429 318 4225 3507
Fragments arranged by size:
4225 3507 429 318
BsrDI GCAATG_nn'
Cuts at: 65 1248 3680 5101 6974 7148 65
Size: 1183 2432 1421 1873 174 1396
Fragments arranged by size:
2432 1873 1421 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 7123 7310 1700
Size: 110 3045 2268 187 2869
Fragments arranged by size:
3045 2869 2268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8479
BssSI C'ACGA_G
Cuts at: 6539 7551 6539
Size: 1012 7467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5096 794
Size: 4302 4177
BstBI TT'CG_AA
Cuts at: 7283 7283
Size: 8479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8479
BstYI r'GATC_y
Cuts at: 1972 3621 4060 4378 5010 5184 5809 6176 6561
Size: 1649 439 318 632 174 625 367 385
Cuts at: 6561 6578 8030 8116 8128 8474 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1649 1452 632 625 439 385 367
346 318 174 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4140 4655 4140
Size: 515 7964
BtgI C'CryG_G
Cuts at: 513 1065 4986 513
Size: 552 3921 4006
Fragments arranged by size:
4006 3921 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2818 487
Size: 683 56 1592 6148
Fragments arranged by size:
6148 1592 683 56
BtsI GCAGTG_nn'
Cuts at: 4957 5995 6789 6809 4957
Size: 1038 794 20 6627
Fragments arranged by size:
6627 1038 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4477 4512 529
Size: 35 3913 35 4496
Fragments arranged by size:
4496 3913 35 35
DraI TTT'AAA
Cuts at: 4387 5783 4387
Size: 1396 7083
DraIII CAC_nnn'GTG
Cuts at: 4631 4631
Size: 8479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7486 809
Size: 507 6170 1802
Fragments arranged by size:
6170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4003 4363 4648
Size: 54 1425 50 275 18 2173 360 285
Cuts at: 4648 5677 5926 6815 8444 8
Size: 1029 249 889 1629 43
Fragments arranged by size:
2173 1629 1425 1029 889 360 285 275
249 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4648 5926 1537
Size: 275 2836 1278 4090
Fragments arranged by size:
4090 2836 1278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2535 3027 4190 4866 6409 1353
Size: 1182 492 1163 676 1543 3423
Fragments arranged by size:
3423 1543 1182 1163 676 492
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7066 7440 7586 387
Size: 1064 138 5477 374 146 1280
Fragments arranged by size:
5477 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2389 4920 2389
Size: 2531 5948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2375 2618 3353 3482 4326 4564
Size: 393 605 138 243 735 129 844 238
Cuts at: 4564 4683 5720 5760 6542 7139 7926 8194 1239
Size: 119 1037 40 782 597 787 268 1524
Fragments arranged by size:
1524 1037 844 787 782 735 605 597
393 268 243 238 138 129 119 40
EcoNI CCTnn'n_nnAGG
Cuts at: 5246 5246
Size: 8479
EcoO109I rG'GnC_Cy
Cuts at: 2072 2185 2335 2906 4096 5373 2072
Size: 113 150 571 1190 1277 5178
Fragments arranged by size:
5178 1277 1190 571 150 113
EcoRI G'AATT_C
Cuts at: 5788 6166 5788
Size: 378 8101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2521 3383 2521
Size: 862 7617
FspI TGC'GCA
Cuts at: 6985 6985
Size: 8479
HaeII r_GCGC'y
Cuts at: 1678 7626 1678
Size: 5948 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2334 2366 3277 3309 5169
Size: 32 135 32 762 32 911 32 1860
Cuts at: 5169 5193 5201 5225 5282 5314 7120 7152 7194
Size: 24 8 24 57 32 1806 32 42
Cuts at: 7194 7226 1373
Size: 32 2626
Fragments arranged by size:
2626 1860 1806 911 762 135 57 42
32 32 32 32 32 32 32 24
24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 4027 5823 6069 669
Size: 988 281 717 1372 1796 246 3079
Fragments arranged by size:
3079 1796 1372 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8479
HpaI GTT'AAC
Cuts at: 6069 6069
Size: 8479
KpnI G_GTAC'C
Cuts at: 5804 5804
Size: 8479
MfeI C'AATT_G
Cuts at: 6078 6078
Size: 8479
MluI A'CGCG_T
Cuts at: 8355 8355
Size: 8479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2116 3451 5108 7593 7777 1305
Size: 811 1335 1657 2485 184 2007
Fragments arranged by size:
2485 2007 1657 1335 811 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4005 4365 10
Size: 54 1425 343 2173 360 4124
Fragments arranged by size:
4124 2173 1425 360 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3603 4304 4988 6437 6796 6955 518
Size: 648 2437 701 684 1449 359 159 2042
Fragments arranged by size:
2437 2042 1449 701 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2162 2280 3851 4929 4941 4971 5157 5895
Size: 556 118 1571 1078 12 30 186 738
Cuts at: 5895 6572 7720 7965 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1571 1148 1078 738 677 556 245
186 118 30 12
NaeI GCC'GGC
Cuts at: 4857 4857
Size: 8479
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7927
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8479
NgoMIV G'CCGG_C
Cuts at: 4855 4855
Size: 8479
NheI G'CTAG_C
Cuts at: 1052 3788 1052
Size: 2736 5743
NotI GC'GGCC_GC
Cuts at: 5926 5926
Size: 8479
NsiI A_TGCA'T
Cuts at: 5101 5101
Size: 8479
NspI r_CATG'y
Cuts at: 1132 1229 3608 4414 5103 5837 7382 1132
Size: 97 2379 806 689 734 1545 2229
Fragments arranged by size:
2379 2229 1545 806 734 689 97
PasI CC'CwG_GG
Cuts at: 1348 2077 1348
Size: 729 7750
PciI A'CATG_T
Cuts at: 7378 7378
Size: 8479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2597 4117 4700 4720 5729 1108
Size: 1489 1520 583 20 1009 3858
Fragments arranged by size:
3858 1520 1489 1009 583 20
PfoI T'CCnGG_A
Cuts at: 4403 4403
Size: 8479
PmeI GTTT'AAAC
Cuts at: 5783 5783
Size: 8479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6548 6580 8061 8093 6548
Size: 32 1481 32 6934
Fragments arranged by size:
6934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2072 2335 2906 5373 2072
Size: 263 571 2467 5178
Fragments arranged by size:
5178 2467 571 263
PshAI GACnn'nnGTC
Cuts at: 1613 4429 1613
Size: 2816 5663
PsiI TTA'TAA
Cuts at: 6049 6049
Size: 8479
PspOMI G'GGCC_C
Cuts at: 2185 4395 2185
Size: 2210 6269
PspXI vC'TCGA_Gb
Cuts at: 2513 2513
Size: 8479
PstI C_TGCA'G
Cuts at: 830 2893 4330 5831 830
Size: 2063 1437 1501 3478
Fragments arranged by size:
3478 2063 1501 1437
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8479
PvuII CAG'CTG
Cuts at: 2280 2280
Size: 8479
SacI G_AGCT'C
Cuts at: 2391 4922 2391
Size: 2531 5948
SalI G'TCGA_C
Cuts at: 1936 2653 5821 1936
Size: 717 3168 4594
Fragments arranged by size:
4594 3168 717
SanDI GG'GwC_CC
Cuts at: 2072 5373 2072
Size: 3301 5178
SapI GCTCTTCn'nnn_
Cuts at: 4190 4190
Size: 8479
SbfI CC_TGCA'GG
Cuts at: 5831 5831
Size: 8479
ScaI AGT'ACT
Cuts at: 6727 8439 6727
Size: 1712 6767
SfcI C'TryA_G
Cuts at: 826 1045 2889 4326 5655 5827 6962 7643 7834
Size: 219 1844 1437 1329 172 1135 681 191
Cuts at: 7834 826
Size: 1471
Fragments arranged by size:
1844 1471 1437 1329 1135 681 219 191
172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8479
SmaI CCC'GGG
Cuts at: 1872 5806 1872
Size: 3934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 3206 3609 5649 6583 7484
Size: 197 926 570 693 403 2040 934 901
Cuts at: 7484 7746 820
Size: 262 1553
Fragments arranged by size:
2040 1553 934 926 901 693 570 403
262 197
SnaBI TAC'GTA
Cuts at: 493 6270 493
Size: 5777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8479
SphI G_CATG'C
Cuts at: 1132 5837 1132
Size: 4705 3774
SspI AAT'ATT
Cuts at: 5 52 6403 5
Size: 47 6351 2081
Fragments arranged by size:
6351 2081 47
StuI AGG'CCT
Cuts at: 1517 4047 1517
Size: 2530 5949
StyI C'CwwG_G
Cuts at: 513 1065 4034 5487 5918 513
Size: 552 2969 1453 431 3074
Fragments arranged by size:
3074 2969 1453 552 431
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2587 4568 4619 6506 6523 6676 6861
Size: 1255 1007 1981 51 1887 17 153 185
Cuts at: 6861 7306 325
Size: 445 1498
Fragments arranged by size:
1981 1887 1498 1255 1007 445 185 153
51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6725 8437 96
Size: 274 80 33 51 1443 4748 1712 138
Fragments arranged by size:
4748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4241 4268 4273 4293 4300 4325 4564 4596 8439
Size: 27 5 20 7 25 239 32 3843
Cuts at: 8439 8471 4241
Size: 32 4249
Fragments arranged by size:
4249 3843 239 32 32 27 25 20
7 5
Tth111I GACn'n_nGTC
Cuts at: 1316 3119 1316
Size: 1803 6676
XbaI T'CTAG_A
Cuts at: 3181 5815 3181
Size: 2634 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2594 2594
Size: 8479
XhoI C'TCGA_G
Cuts at: 1943 2513 5649 1943
Size: 570 3136 4773
Fragments arranged by size:
4773 3136 570
XmaI C'CCGG_G
Cuts at: 1870 5804 1870
Size: 3934 4545
XmnI GAAnn'nnTTC
Cuts at: 2501 3397 3623 5751 6608 2501
Size: 896 226 2128 857 4372
Fragments arranged by size:
4372 2128 896 857 226
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6287 276
Size: 53 83 186 936 4753 2468
Fragments arranged by size:
4753 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI