Restriction map for
pFN21AB9404
(Circular) (Six-base) MAPSORT of: pFN21AB9404.seq Check: 5304 from: 1 to: 7111
pFN21AB9404 7111 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4921 278
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2998 4454 1937
Size: 1061 1456 4594
Fragments arranged by size:
4594 1456 1061
Acc65I G'GTAC_C
Cuts at: 4432 4432
Size: 7111
AclI AA'CG_TT
Cuts at: 1563 5238 5611 1563
Size: 3675 373 3063
Fragments arranged by size:
3675 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 3711 5174 6558 6826 1632
Size: 674 1405 1463 1384 268 1917
Fragments arranged by size:
1917 1463 1405 1384 674 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6914
AflIII A'CryG_T
Cuts at: 1203 3901 6010 6987 1203
Size: 2698 2109 977 1327
Fragments arranged by size:
2698 2109 1327 977
AgeI A'CCGG_T
Cuts at: 5942 5942
Size: 7111
AhdI GACnn_n'nnGTC
Cuts at: 4148 5840 4148
Size: 1692 5419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7111
AlwNI CAG_nnn'CTG
Cuts at: 4854 6426 4854
Size: 1572 5539
ApaLI G'TGCA_C
Cuts at: 5168 6324 5168
Size: 1156 5955
ApoI r'AATT_y
Cuts at: 1493 2425 4420 4646 4798 6907 1493
Size: 932 1995 226 152 2109 1697
Fragments arranged by size:
2109 1995 1697 932 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2823 3555 4370 5665 160
Size: 872 1791 732 815 1295 1606
Fragments arranged by size:
1791 1606 1295 872 815 732
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
AvaI C'yCGr_G
Cuts at: 1870 1943 2919 4436 1870
Size: 73 976 1517 4545
Fragments arranged by size:
4545 1517 976 73
BamHI G'GATC_C
Cuts at: 3682 3830 4441 4808 3682
Size: 148 611 367 5985
Fragments arranged by size:
5985 611 367 148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4432 5887 618
Size: 325 217 627 2645 1455 1842
Fragments arranged by size:
2645 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2810 4054 4430 1391
Size: 1419 1244 376 4072
Fragments arranged by size:
4072 1419 1244 376
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3213 3321 928
Size: 971 1314 108 4718
Fragments arranged by size:
4718 1314 971 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2287 2321 3776 3810 5302 5336 1903
Size: 34 350 34 1455 34 1492 34 3678
Fragments arranged by size:
3678 1492 1455 350 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5003 6213 5003
Size: 1210 5901
BclI T'GATC_A
Cuts at: 1548 2989 1548
Size: 1441 5670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5722 136
Size: 107 122 71 631 605 4050 1525
Fragments arranged by size:
4050 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7106 7106
Size: 7111
BlpI GC'TnA_GC
Cuts at: 4528 4528
Size: 7111
Bme1580I G_kGCm'C
Cuts at: 1790 5172 6328 1790
Size: 3382 1156 2573
Fragments arranged by size:
3382 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7111
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2215 2430 2773
Size: 416 53 83 399 24 798 215 343
Cuts at: 2773 4842 5800 442
Size: 2069 958 1753
Fragments arranged by size:
2069 1753 958 798 416 399 343 215
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4068 5771 1239
Size: 2829 1703 2579
Fragments arranged by size:
2829 2579 1703
Bpu10I CC'TnA_GC
Cuts at: 2355 2465 2854 2355
Size: 110 389 6612
Fragments arranged by size:
6612 389 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2960 3360 3422 5236 6101 6399 2960
Size: 400 62 1814 865 298 3672
Fragments arranged by size:
3672 1814 865 400 298 62
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5774 882
Size: 617 4275 2219
Fragments arranged by size:
4275 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4408 4902 493
Size: 711 3204 494 2702
Fragments arranged by size:
3204 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2418 3252 7105 2418
Size: 834 3853 2424
Fragments arranged by size:
3853 2424 834
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3775 4918 5300 5935
Size: 53 83 186 936 2242 1143 382 635
Cuts at: 5935 275
Size: 1451
Fragments arranged by size:
2242 1451 1143 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3634 5544 5942 6216 6363 6774 3634
Size: 1910 398 274 147 411 3971
Fragments arranged by size:
3971 1910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2701 2731 1178
Size: 30 165 30 1298 30 5558
Fragments arranged by size:
5558 1298 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2898 3220 3641 1186
Size: 1712 322 421 4656
Fragments arranged by size:
4656 1712 421 322
BseYI C'CCAG_C
Cuts at: 3066 6314 6810 3066
Size: 3248 496 3367
Fragments arranged by size:
3367 3248 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3036 1163
Size: 573 1300 5238
Fragments arranged by size:
5238 1300 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4561 5322 6350 1540
Size: 275 186 2560 761 1028 2301
Fragments arranged by size:
2560 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5172 5257 6328 5172
Size: 85 1071 5955
Fragments arranged by size:
5955 1071 85
BsmI GAATG_Cn'
Cuts at: 4622 4715 4622
Size: 93 7018
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2810 4054 4430 5172 5257 6328 1391
Size: 399 1020 1244 376 742 85 1071 2174
Fragments arranged by size:
2174 1244 1071 1020 742 399 376 85
BspEI T'CCGG_A
Cuts at: 6774 6774
Size: 7111
BspHI T'CATG_A
Cuts at: 3991 5000 6730 3991
Size: 1009 1730 4372
Fragments arranged by size:
4372 1730 1009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2260 4466 844
Size: 1416 2206 3489
Fragments arranged by size:
3489 2206 1416
BsrBI CCG'CTC
Cuts at: 1394 1823 4998 1394
Size: 429 3175 3507
Fragments arranged by size:
3507 3175 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5606 5780 65
Size: 1183 4358 174 1396
Fragments arranged by size:
4358 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5755 5942 1700
Size: 110 3945 187 2869
Fragments arranged by size:
3945 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7111
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7111
BssSI C'ACGA_G
Cuts at: 5171 6183 5171
Size: 1012 6099
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 794
Size: 1520 5591
BstBI TT'CG_AA
Cuts at: 2007 3313 5915 2007
Size: 1306 2602 3203
Fragments arranged by size:
3203 2602 1306
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7111
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 3160 2029
Size: 1131 5980
BstYI r'GATC_y
Cuts at: 1972 3023 3682 3830 4441 4808 5193 5210 6662
Size: 1051 659 148 611 367 385 17 1452
Cuts at: 6662 6748 6760 7106 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1051 659 611 385 367 346
148 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6559
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6372
Fragments arranged by size:
6372 683 56
BtsI GCAGTG_nn'
Cuts at: 3966 4627 5421 5441 3966
Size: 661 794 20 5636
Fragments arranged by size:
5636 794 661 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7076
DraI TTT'AAA
Cuts at: 3568 3706 4415 3568
Size: 138 709 6264
Fragments arranged by size:
6264 709 138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6118 809
Size: 507 4802 1802
Fragments arranged by size:
4802 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4558 5447 7076
Size: 54 1425 50 275 18 2728 889 1629
Cuts at: 7076 8
Size: 43
Fragments arranged by size:
2728 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4558 1537
Size: 275 2746 4090
Fragments arranged by size:
4090 2746 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 3618 4225 5041 1353
Size: 1440 825 607 816 3423
Fragments arranged by size:
3423 1440 825 816 607
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5698 6072 6218 387
Size: 1064 138 4109 374 146 1280
Fragments arranged by size:
4109 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 3711 4068 5174 5771 6558 6826
Size: 393 674 1405 357 1106 597 787 268
Cuts at: 6826 1239
Size: 1524
Fragments arranged by size:
1524 1405 1106 787 674 597 393 357
268
EcoO109I rG'GnC_Cy
Cuts at: 4165 4165
Size: 7111
EcoRI G'AATT_C
Cuts at: 4420 4798 4420
Size: 378 6733
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3769 3769
Size: 7111
FspI TGC'GCA
Cuts at: 5617 5617
Size: 7111
HaeII r_GCGC'y
Cuts at: 1678 6258 1678
Size: 4580 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 2302 2334 2648
Size: 32 135 32 608 32 90 32 314
Cuts at: 2648 2680 3164 3196 3822 3854 4134 4166 5752
Size: 32 484 32 626 32 280 32 1586
Cuts at: 5752 5784 5826 5858 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1586 626 608 484 314 280 135
90 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2461 4455 4701 669
Size: 988 281 523 1994 246 3079
Fragments arranged by size:
3079 1994 988 523 281 246
HindIII A'AGCT_T
Cuts at: 748 3692 748
Size: 2944 4167
HpaI GTT'AAC
Cuts at: 4701 4701
Size: 7111
KpnI G_GTAC'C
Cuts at: 4436 4436
Size: 7111
MfeI C'AATT_G
Cuts at: 3450 4281 4710 3450
Size: 831 429 5851
Fragments arranged by size:
5851 831 429
MluI A'CGCG_T
Cuts at: 6987 6987
Size: 7111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2894 3209 4243 6225 6409 1305
Size: 1589 315 1034 1982 184 2007
Fragments arranged by size:
2007 1982 1589 1034 315 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5289
Fragments arranged by size:
5289 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2147 2985 4107 5069 5428 5587
Size: 648 861 120 838 1122 962 359 159
Cuts at: 5587 518
Size: 2042
Fragments arranged by size:
2042 1122 962 861 838 648 359 159
120
MspA1I CmG'CkG
Cuts at: 1606 4527 5204 6352 6597 1606
Size: 2921 677 1148 245 2120
Fragments arranged by size:
2921 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6559
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7111
NotI GC'GGCC_GC
Cuts at: 4558 4558
Size: 7111
NspI r_CATG'y
Cuts at: 1132 1229 2984 3905 4469 6014 1132
Size: 97 1755 921 564 1545 2229
Fragments arranged by size:
2229 1755 1545 921 564 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7111
PciI A'CATG_T
Cuts at: 3901 6010 3901
Size: 2109 5002
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2801 1108
Size: 1693 5418
PfoI T'CCnGG_A
Cuts at: 2056 2056
Size: 7111
PmeI GTTT'AAAC
Cuts at: 4415 4415
Size: 7111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5180 5212 6693 6725 5180
Size: 32 1481 32 5566
Fragments arranged by size:
5566 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4165 4165
Size: 7111
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7111
PsiI TTA'TAA
Cuts at: 2442 4681 2442
Size: 2239 4872
PstI C_TGCA'G
Cuts at: 830 4463 830
Size: 3633 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7111
SalI G'TCGA_C
Cuts at: 1936 4453 1936
Size: 2517 4594
SapI GCTCTTCn'nnn_
Cuts at: 2793 2793
Size: 7111
SbfI CC_TGCA'GG
Cuts at: 4463 4463
Size: 7111
ScaI AGT'ACT
Cuts at: 5359 7071 5359
Size: 1712 5399
SfcI C'TryA_G
Cuts at: 826 1045 3010 4459 5594 6275 6466 826
Size: 219 1965 1449 1135 681 191 1471
Fragments arranged by size:
1965 1471 1449 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7111
SmaI CCC'GGG
Cuts at: 1872 4438 1872
Size: 2566 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2975 3375 3437 5215 6116 6378
Size: 197 926 1032 400 62 1778 901 262
Cuts at: 6378 820
Size: 1553
Fragments arranged by size:
1778 1553 1032 926 901 400 262 197
62
SnaBI TAC'GTA
Cuts at: 493 4408 4902 493
Size: 3915 494 2702
Fragments arranged by size:
3915 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7111
SphI G_CATG'C
Cuts at: 1132 4469 1132
Size: 3337 3774
SspI AAT'ATT
Cuts at: 5 52 3005 5035 5
Size: 47 2953 2030 2081
Fragments arranged by size:
2953 2081 2030 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7111
StyI C'CwwG_G
Cuts at: 513 1065 4550 513
Size: 552 3485 3074
Fragments arranged by size:
3485 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3018 5138 5155 5308 5493 5938 325
Size: 1255 1438 2120 17 153 185 445 1498
Fragments arranged by size:
2120 1498 1438 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3581 5357 7069
Size: 274 80 33 51 1443 1604 1776 1712
Cuts at: 7069 96
Size: 138
Fragments arranged by size:
1776 1712 1604 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2954 2986 7071 7103 2954
Size: 32 4085 32 2962
Fragments arranged by size:
4085 2962 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7111
XbaI T'CTAG_A
Cuts at: 4447 4447
Size: 7111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7111
XmaI C'CCGG_G
Cuts at: 1870 4436 1870
Size: 2566 4545
XmnI GAAnn'nnTTC
Cuts at: 4032 4371 5240 4032
Size: 339 869 5903
Fragments arranged by size:
5903 869 339
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4919 276
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI