Restriction map for
pFN21AB9402
(Circular) (Six-base) MAPSORT of: pFN21AB9402.seq Check: 2677 from: 1 to: 6430
pFN21AB9402 6430 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2026 2314 2026
Size: 288 6142
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2901 3321 3773 1937
Size: 964 420 452 4594
Fragments arranged by size:
4594 964 452 420
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 2649 4557 4930 1563
Size: 1086 1908 373 3063
Fragments arranged by size:
3063 1908 1086 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4493 5877 6145 1632
Size: 2861 1384 268 1917
Fragments arranged by size:
2861 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 3727 5261 3727
Size: 1534 4896
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 3307 3565 4173 5745 3307
Size: 258 608 1572 3992
Fragments arranged by size:
3992 1572 608 258
ApaI G_GGCC'C
Cuts at: 2180 2180
Size: 6430
ApaLI G'TGCA_C
Cuts at: 2327 2888 4487 5643 2327
Size: 561 1599 1156 3114
Fragments arranged by size:
3114 1599 1156 561
ApoI r'AATT_y
Cuts at: 1493 3739 3965 4117 6226 1493
Size: 2246 226 152 2109 1697
Fragments arranged by size:
2246 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 3413 3755 1870
Size: 73 271 1199 342 4545
Fragments arranged by size:
4545 1199 342 271 73
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3587 3751 5206 618
Size: 325 217 627 1800 164 1455 1842
Fragments arranged by size:
1842 1800 1455 627 325 217 164
BanII G_rGCy'C
Cuts at: 1391 2180 2225 2775 2840 3749 1391
Size: 789 45 550 65 909 4072
Fragments arranged by size:
4072 909 789 550 65 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2699 3347 928
Size: 971 800 648 4011
Fragments arranged by size:
4011 971 800 648
BbvCI CC'TCA_GC
Cuts at: 2910 2910
Size: 6430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4621 4655 1903
Size: 34 2684 34 3678
Fragments arranged by size:
3678 2684 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2835 5041 136
Size: 107 122 71 631 605 1163 2206 1525
Fragments arranged by size:
2206 1525 1163 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 3847 3847
Size: 6430
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2180 2331 2892 4491 5647 1790
Size: 379 11 151 561 1599 1156 2573
Fragments arranged by size:
2573 1599 1156 561 379 151 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2401 2512 2776
Size: 416 53 83 399 24 984 111 264
Cuts at: 2776 2978 4161 5119 442
Size: 202 1183 958 1753
Fragments arranged by size:
1753 1183 984 958 416 399 264 202
111 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2508 3096 3311 3329 3518 5090 1239
Size: 1269 588 215 18 189 1572 2579
Fragments arranged by size:
2579 1572 1269 588 215 189 18
Bpu10I CC'TnA_GC
Cuts at: 2508 2910 2508
Size: 402 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4555 5420 5718 4555
Size: 865 298 5267
Fragments arranged by size:
5267 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5093 882
Size: 617 3594 2219
Fragments arranged by size:
3594 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2148 4221 493
Size: 711 944 2073 2702
Fragments arranged by size:
2702 2073 944 711
BsaBI GATnn'nnATC
Cuts at: 2090 6424 2090
Size: 4334 2096
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 4237 4619 5254
Size: 53 83 186 936 509 2195 382 635
Cuts at: 5254 275
Size: 1451
Fragments arranged by size:
2195 1451 936 635 509 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2629 3727 4863 5261 5535 5682 6093 2629
Size: 1098 1136 398 274 147 411 2966
Fragments arranged by size:
2966 1136 1098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2982 3012 3436 3466 1178
Size: 30 165 30 1579 30 424 30 4142
Fragments arranged by size:
4142 1579 424 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2385 2545 3117 1186
Size: 1199 160 572 4499
Fragments arranged by size:
4499 1199 572 160
BseYI C'CCAG_C
Cuts at: 2265 2349 2484 2491 2667 2704 2949 3131 3197
Size: 84 135 7 176 37 245 182 66
Cuts at: 3197 3539 3651 3689 3718 5633 6129 2265
Size: 342 112 38 29 1915 496 2566
Fragments arranged by size:
2566 1915 496 342 245 182 176 135
112 84 66 38 37 29 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2922 1163
Size: 573 1186 4671
Fragments arranged by size:
4671 1186 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3880 4641 5669 1540
Size: 275 186 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 2331 2892 4491 4576 5647 2301
Size: 30 561 1599 85 1071 3084
Fragments arranged by size:
3084 1599 1071 561 85 30
BsmI GAATG_Cn'
Cuts at: 3941 4034 3941
Size: 93 6337
BsmBI CGTCTCn'nnnn_
Cuts at: 3441 3441
Size: 6430
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2180 2225 2301 2331 2775 2840
Size: 399 379 11 45 76 30 444 65
Cuts at: 2840 2892 3749 4491 4576 5647 1391
Size: 52 857 742 85 1071 2174
Fragments arranged by size:
2174 1071 857 742 444 399 379 85
76 65 52 45 30 11
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2026 2314 3785 844
Size: 1182 288 1471 3489
Fragments arranged by size:
3489 1471 1182 288
BsrBI CCG'CTC
Cuts at: 1394 1823 2512 4317 1394
Size: 429 689 1805 3507
Fragments arranged by size:
3507 1805 689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 2319 3727 5074 5261 1700
Size: 110 295 214 1408 1347 187 2869
Fragments arranged by size:
2869 1408 1347 295 214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 2639 4490 5502 2639
Size: 1851 1012 3567
Fragments arranged by size:
3567 1851 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6430
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 2054 3713 1184
Size: 870 1659 3901
Fragments arranged by size:
3901 1659 870
BstXI CCAn_nnnn'nTGG
Cuts at: 3172 3172
Size: 6430
BstYI r'GATC_y
Cuts at: 1972 2751 3634 3760 4127 4512 4529 5981 6067
Size: 779 883 126 367 385 17 1452 86
Cuts at: 6067 6079 6425 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 883 779 385 367 346 126
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2500 2609 2895 3591 513
Size: 552 1435 109 286 696 3352
Fragments arranged by size:
3352 1435 696 552 286 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 3201 3563 3946 4740 4760 3201
Size: 362 383 794 20 4871
Fragments arranged by size:
4871 794 383 362 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DraIII CAC_nnn'GTG
Cuts at: 2148 2257 2896 2148
Size: 109 639 5682
Fragments arranged by size:
5682 639 109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2559 2657 3877
Size: 54 1425 50 275 18 729 98 1220
Cuts at: 3877 4766 6395 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1220 889 729 275 98 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3877 1537
Size: 275 2065 4090
Fragments arranged by size:
4090 2065 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3163 3177 3363 4360 1353
Size: 1810 14 186 997 3423
Fragments arranged by size:
3423 1810 997 186 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5017 5391 5537 387
Size: 1064 138 3428 374 146 1280
Fragments arranged by size:
3428 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 3096 3311 3329 3518 4493 5090
Size: 393 876 588 215 18 189 975 597
Cuts at: 5090 5877 6145 1239
Size: 787 268 1524
Fragments arranged by size:
1524 975 876 787 597 588 393 268
215 189 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2578 3293 2578
Size: 715 5715
EcoO109I rG'GnC_Cy
Cuts at: 2176 2177 2403 2176
Size: 1 226 6203
Fragments arranged by size:
6203 226 1
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2013 2313 5577 1678
Size: 335 300 3264 2531
Fragments arranged by size:
3264 2531 335 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 2177 2209 2528
Size: 32 135 32 498 32 75 32 319
Cuts at: 2528 2560 2982 3014 3438 3470 5071 5103 5145
Size: 32 422 32 424 32 1601 32 42
Cuts at: 5145 5177 1373
Size: 32 2626
Fragments arranged by size:
2626 1601 498 424 422 319 135 75
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2902 3774 4020 669
Size: 988 281 964 872 246 3079
Fragments arranged by size:
3079 988 964 872 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2296 2968 5544 5728 1305
Size: 991 672 2576 184 2007
Fragments arranged by size:
2576 2007 991 672 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4608
Fragments arranged by size:
4608 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 2630 2885 3581 4388 4747 4906
Size: 648 1128 336 255 696 807 359 159
Cuts at: 4906 518
Size: 2042
Fragments arranged by size:
2042 1128 807 696 648 359 336 255
159
MspA1I CmG'CkG
Cuts at: 1606 2390 2471 2528 2611 3846 4523 5671 5916
Size: 784 81 57 83 1235 677 1148 245
Cuts at: 5916 1606
Size: 2120
Fragments arranged by size:
2120 1235 1148 784 677 245 83 81
57
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5878
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 2428 3788 5333 1132
Size: 97 1199 1360 1545 2229
Fragments arranged by size:
2229 1545 1360 1199 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6430
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2148 1108
Size: 1040 5390
PfoI T'CCnGG_A
Cuts at: 3004 3262 3288 3004
Size: 258 26 6146
Fragments arranged by size:
6146 258 26
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2403 2403
Size: 6430
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2176 2176
Size: 6430
PstI C_TGCA'G
Cuts at: 830 3214 3570 3782 830
Size: 2384 356 212 3478
Fragments arranged by size:
3478 2384 356 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2528 2528
Size: 6430
SacII CC_GC'GG
Cuts at: 2612 2612
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 2900 3772 1936
Size: 964 872 4594
Fragments arranged by size:
4594 964 872
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SfcI C'TryA_G
Cuts at: 826 1045 3177 3210 3318 3566 3778 4913 5594
Size: 219 2132 33 108 248 212 1135 681
Cuts at: 5594 5785 826
Size: 191 1471
Fragments arranged by size:
2132 1471 1135 681 248 219 212 191
108 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 2216 3757 1872
Size: 344 1541 4545
Fragments arranged by size:
4545 1541 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4534 5435 5697 820
Size: 197 926 2591 901 262 1553
Fragments arranged by size:
2591 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 2428 3788 1132
Size: 1296 1360 3774
Fragments arranged by size:
3774 1360 1296
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 2908 1517
Size: 1391 5039
StyI C'CwwG_G
Cuts at: 513 1065 2181 3869 513
Size: 552 1116 1688 3074
Fragments arranged by size:
3074 1688 1116 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2033 3575 4457 4474 4627 4812 5257
Size: 1255 453 1542 882 17 153 185 445
Cuts at: 5257 325
Size: 1498
Fragments arranged by size:
1542 1498 1255 882 453 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4676 6388 96
Size: 274 80 33 51 1443 2699 1712 138
Fragments arranged by size:
2699 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3436 3468 6390 6422 3436
Size: 32 2922 32 3444
Fragments arranged by size:
3444 2922 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3452 1316
Size: 2136 4294
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 3388 2035
Size: 1353 5077
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 2214 3755 1870
Size: 344 1541 4545
Fragments arranged by size:
4545 1541 344
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI