Restriction map for
pFN21AB9389
(Circular) (Six-base) MAPSORT of: pFN21AB9389.seq Check: 7893 from: 1 to: 6790
pFN21AB9389 6790 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4600 278
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2534 3208 4133 1937
Size: 597 674 925 4594
Fragments arranged by size:
4594 925 674 597
Acc65I G'GTAC_C
Cuts at: 4111 4111
Size: 6790
AclI AA'CG_TT
Cuts at: 1563 4917 5290 1563
Size: 3354 373 3063
Fragments arranged by size:
3354 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2526 2535 3054 3606 4853 6237 6505 1632
Size: 894 9 519 552 1247 1384 268 1917
Fragments arranged by size:
1917 1384 1247 894 552 519 268 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6593
AflIII A'CryG_T
Cuts at: 1203 5689 6666 1203
Size: 4486 977 1327
Fragments arranged by size:
4486 1327 977
AgeI A'CCGG_T
Cuts at: 3096 5621 3096
Size: 2525 4265
AhdI GACnn_n'nnGTC
Cuts at: 2668 5519 2668
Size: 2851 3939
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6790
AlwNI CAG_nnn'CTG
Cuts at: 2108 2705 2879 3032 3170 4533 6105 2108
Size: 597 174 153 138 1363 1572 2793
Fragments arranged by size:
2793 1572 1363 597 174 153 138
ApaLI G'TGCA_C
Cuts at: 4847 6003 4847
Size: 1156 5634
ApoI r'AATT_y
Cuts at: 1493 3551 4099 4325 4477 6586 1493
Size: 2058 548 226 152 2109 1697
Fragments arranged by size:
2109 2058 1697 548 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5344 160
Size: 872 4312 1606
Fragments arranged by size:
4312 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 4115 1870
Size: 73 894 1278 4545
Fragments arranged by size:
4545 1278 894 73
BamHI G'GATC_C
Cuts at: 4120 4487 4120
Size: 367 6423
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2861 4111 5566 618
Size: 325 217 627 1074 1250 1455 1842
Fragments arranged by size:
1842 1455 1250 1074 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2886 3631 4109 1391
Size: 1495 745 478 4072
Fragments arranged by size:
4072 1495 745 478
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3366 3495 928
Size: 971 1467 129 4223
Fragments arranged by size:
4223 1467 971 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4981 5015 1903
Size: 34 3044 34 3678
Fragments arranged by size:
3678 3044 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2809 4682 5892 2809
Size: 1873 1210 3707
Fragments arranged by size:
3707 1873 1210
BclI T'GATC_A
Cuts at: 1548 2827 1548
Size: 1279 5511
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5401 136
Size: 107 122 71 631 605 3729 1525
Fragments arranged by size:
3729 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3934 6785 3934
Size: 2851 3939
BlpI GC'TnA_GC
Cuts at: 4207 4207
Size: 6790
Bme1580I G_kGCm'C
Cuts at: 1790 2864 3248 4851 6007 1790
Size: 1074 384 1603 1156 2573
Fragments arranged by size:
2573 1603 1156 1074 384
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6790
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2867 4521 5479
Size: 416 53 83 399 24 1450 1654 958
Cuts at: 5479 442
Size: 1753
Fragments arranged by size:
1753 1654 1450 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6790
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3538 3538
Size: 6790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3147 3645 5450 1239
Size: 1908 498 1805 2579
Fragments arranged by size:
2579 1908 1805 498
Bpu10I CC'TnA_GC
Cuts at: 2880 3225 2880
Size: 345 6445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4915 5780 6078 4915
Size: 865 298 5627
Fragments arranged by size:
5627 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5453 882
Size: 617 3954 2219
Fragments arranged by size:
3954 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4581 493
Size: 711 3377 2702
Fragments arranged by size:
3377 2702 711
BsaBI GATnn'nnATC
Cuts at: 6784 6784
Size: 6790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2664 4597 4979 5614
Size: 53 83 186 936 1131 1933 382 635
Cuts at: 5614 275
Size: 1451
Fragments arranged by size:
1933 1451 1131 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2434 3096 5223 5621 5895 6042 6453 2434
Size: 662 2127 398 274 147 411 2771
Fragments arranged by size:
2771 2127 662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2328 2358 2593 2623 3170
Size: 30 165 30 925 30 235 30 547
Cuts at: 3170 3200 3382 3412 1178
Size: 30 182 30 4556
Fragments arranged by size:
4556 925 547 235 182 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6790
BseYI C'CCAG_C
Cuts at: 2410 3604 5993 6489 2410
Size: 1194 2389 496 2711
Fragments arranged by size:
2711 2389 1194 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4240 5001 6029 1540
Size: 275 186 2239 761 1028 2301
Fragments arranged by size:
2301 2239 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2886 4851 4936 6007 2886
Size: 1965 85 1071 3669
Fragments arranged by size:
3669 1965 1071 85
BsmI GAATG_Cn'
Cuts at: 4301 4394 4301
Size: 93 6697
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2864 2886 3248 3631 4109 4851 4936
Size: 399 1074 22 362 383 478 742 85
Cuts at: 4936 6007 1391
Size: 1071 2174
Fragments arranged by size:
2174 1074 1071 742 478 399 383 362
85 22
BspEI T'CCGG_A
Cuts at: 6453 6453
Size: 6790
BspHI T'CATG_A
Cuts at: 2146 4679 6409 2146
Size: 2533 1730 2527
Fragments arranged by size:
2533 2527 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3904 4145 844
Size: 3060 241 3489
Fragments arranged by size:
3489 3060 241
BsrBI CCG'CTC
Cuts at: 1394 1823 2895 4677 1394
Size: 429 1072 1782 3507
Fragments arranged by size:
3507 1782 1072 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5285 5459 65
Size: 1183 4037 174 1396
Fragments arranged by size:
4037 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3096 3229 5434 5621 1700
Size: 110 1286 133 2205 187 2869
Fragments arranged by size:
2869 2205 1286 187 133 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6790
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6790
BssSI C'ACGA_G
Cuts at: 4850 5862 4850
Size: 1012 5778
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6790
BstBI TT'CG_AA
Cuts at: 5594 5594
Size: 6790
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6790
BstYI r'GATC_y
Cuts at: 1972 2554 3304 3934 4120 4487 4872 4889 6341
Size: 582 750 630 186 367 385 17 1452
Cuts at: 6341 6427 6439 6785 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 750 630 582 385 367 346
186 86 17 12
BstZ17I GTA'TAC
Cuts at: 2535 3209 2535
Size: 674 6116
BtgI C'CryG_G
Cuts at: 513 1065 2997 513
Size: 552 1932 4306
Fragments arranged by size:
4306 1932 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6051
Fragments arranged by size:
6051 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 4306 5100 5120 2355
Size: 1951 794 20 4025
Fragments arranged by size:
4025 1951 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6755
DraI TTT'AAA
Cuts at: 2745 4094 2745
Size: 1349 5441
DraIII CAC_nnn'GTG
Cuts at: 3638 3638
Size: 6790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5797 809
Size: 507 4481 1802
Fragments arranged by size:
4481 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4237 5126 6755
Size: 54 1425 50 275 18 2407 889 1629
Cuts at: 6755 8
Size: 43
Fragments arranged by size:
2407 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4237 1537
Size: 275 2425 4090
Fragments arranged by size:
4090 2425 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3411 4720 1353
Size: 2058 1309 3423
Fragments arranged by size:
3423 2058 1309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5377 5751 5897 387
Size: 1064 138 3788 374 146 1280
Fragments arranged by size:
3788 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2884 2884
Size: 6790
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2526 2535 3054 3147 3606 3645 4853
Size: 393 894 9 519 93 459 39 1208
Cuts at: 4853 5450 6237 6505 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1208 894 787 597 519 459 393
268 93 39 9
EcoO109I rG'GnC_Cy
Cuts at: 2501 2501
Size: 6790
EcoRI G'AATT_C
Cuts at: 4099 4477 4099
Size: 378 6412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3512 3512
Size: 6790
FspI TGC'GCA
Cuts at: 5296 5296
Size: 6790
HaeII r_GCGC'y
Cuts at: 1678 5937 1678
Size: 4259 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2141 2173 2429
Size: 32 135 32 530 32 7 32 256
Cuts at: 2429 2461 2654 2686 2831 2863 2879 2911 3104
Size: 32 193 32 145 32 16 32 193
Cuts at: 3104 3136 3170 3202 3494 3526 3671 3703 3911
Size: 32 34 32 292 32 145 32 208
Cuts at: 3911 3943 5431 5463 5505 5537 1373
Size: 32 1488 32 42 32 2626
Fragments arranged by size:
2626 1488 530 292 256 208 193 193
145 145 135 42 34 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 16 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2127 4134 4380 669
Size: 988 281 189 2007 246 3079
Fragments arranged by size:
3079 2007 988 281 246 189
HindIII A'AGCT_T
Cuts at: 748 3371 3782 748
Size: 2623 411 3756
Fragments arranged by size:
3756 2623 411
HpaI GTT'AAC
Cuts at: 4380 4380
Size: 6790
KpnI G_GTAC'C
Cuts at: 4115 4115
Size: 6790
MfeI C'AATT_G
Cuts at: 4389 4389
Size: 6790
MluI A'CGCG_T
Cuts at: 6666 6666
Size: 6790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3156 5904 6088 1305
Size: 1851 2748 184 2007
Fragments arranged by size:
2748 2007 1851 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4968
Fragments arranged by size:
4968 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4748 5107 5266 518
Size: 648 3582 359 159 2042
Fragments arranged by size:
3582 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2267 2414 2702 3158 3945 4206 4883 6031
Size: 661 147 288 456 787 261 677 1148
Cuts at: 6031 6276 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 787 677 661 456 288 261
245 147
NaeI GCC'GGC
Cuts at: 3231 3231
Size: 6790
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6238
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6790
NgoMIV G'CCGG_C
Cuts at: 3229 3229
Size: 6790
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6790
NotI GC'GGCC_GC
Cuts at: 4237 4237
Size: 6790
NspI r_CATG'y
Cuts at: 1132 1229 4148 5693 1132
Size: 97 2919 1545 2229
Fragments arranged by size:
2919 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6790
PciI A'CATG_T
Cuts at: 5689 5689
Size: 6790
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 3314 1108
Size: 939 1267 4584
Fragments arranged by size:
4584 1267 939
PfoI T'CCnGG_A
Cuts at: 3828 3828
Size: 6790
PmeI GTTT'AAAC
Cuts at: 4094 4094
Size: 6790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2868 2900 4859 4891 6372 6404 2868
Size: 32 1959 32 1481 32 3254
Fragments arranged by size:
3254 1959 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2501 2501
Size: 6790
PshAI GACnn'nnGTC
Cuts at: 1613 3945 1613
Size: 2332 4458
PsiI TTA'TAA
Cuts at: 4360 4360
Size: 6790
PspXI vC'TCGA_Gb
Cuts at: 2837 2837
Size: 6790
PstI C_TGCA'G
Cuts at: 830 3901 4142 830
Size: 3071 241 3478
Fragments arranged by size:
3478 3071 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6790
PvuII CAG'CTG
Cuts at: 2267 2414 2702 3945 2267
Size: 147 288 1243 5112
Fragments arranged by size:
5112 1243 288 147
SacI G_AGCT'C
Cuts at: 2886 2886
Size: 6790
SalI G'TCGA_C
Cuts at: 1936 4132 1936
Size: 2196 4594
SbfI CC_TGCA'GG
Cuts at: 4142 4142
Size: 6790
ScaI AGT'ACT
Cuts at: 5038 6750 5038
Size: 1712 5078
SfcI C'TryA_G
Cuts at: 826 1045 2658 2697 2733 3264 3897 3957 4138
Size: 219 1613 39 36 531 633 60 181
Cuts at: 4138 5273 5954 6145 826
Size: 1135 681 191 1471
Fragments arranged by size:
1613 1471 1135 681 633 531 219 191
181 60 39 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6790
SmaI CCC'GGG
Cuts at: 1872 4117 1872
Size: 2245 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2837 4894 5795 6057 820
Size: 197 926 894 2057 901 262 1553
Fragments arranged by size:
2057 1553 926 901 894 262 197
SnaBI TAC'GTA
Cuts at: 493 4581 493
Size: 4088 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6790
SphI G_CATG'C
Cuts at: 1132 4148 1132
Size: 3016 3774
SspI AAT'ATT
Cuts at: 5 52 4714 5
Size: 47 4662 2081
Fragments arranged by size:
4662 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6790
StyI C'CwwG_G
Cuts at: 513 1065 2022 3273 3675 4229 513
Size: 552 957 1251 402 554 3074
Fragments arranged by size:
3074 1251 957 554 552 402
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3493 4817 4834 4987 5172 5617 325
Size: 1255 1913 1324 17 153 185 445 1498
Fragments arranged by size:
1913 1498 1324 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2979 5036 6748
Size: 274 80 33 51 1443 1002 2057 1712
Cuts at: 6748 96
Size: 138
Fragments arranged by size:
2057 1712 1443 1002 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2593 2625 3309 3341 6750 6782 2593
Size: 32 684 32 3409 32 2601
Fragments arranged by size:
3409 2601 684 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6790
XbaI T'CTAG_A
Cuts at: 4126 4126
Size: 6790
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2137 3586 2137
Size: 1449 5341
XhoI C'TCGA_G
Cuts at: 1943 2837 1943
Size: 894 5896
XmaI C'CCGG_G
Cuts at: 1870 4115 1870
Size: 2245 4545
XmnI GAAnn'nnTTC
Cuts at: 3393 3471 4919 3393
Size: 78 1448 5264
Fragments arranged by size:
5264 1448 78
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4598 276
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI