Restriction map for
pFN21AB9385
(Circular) (Six-base) MAPSORT of: pFN21AB9385.seq Check: 5561 from: 1 to: 5677
pFN21AB9385 5677 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2826 2998 2826
Size: 172 5505
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2839 2946 3740 5124 5392 1632
Size: 1207 107 794 1384 268 1917
Fragments arranged by size:
1917 1384 1207 794 268 107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 2112 4576 5553 1203
Size: 909 2464 977 1327
Fragments arranged by size:
2464 1327 977 909
AgeI A'CCGG_T
Cuts at: 2009 4508 2009
Size: 2499 3178
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 2117 1166
Size: 951 4726
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 2092 2441 3420 4992 2092
Size: 349 979 1572 2777
Fragments arranged by size:
2777 1572 979 349
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2986 3212 3364 5473 1493
Size: 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 3002 1870
Size: 73 99 960 4545
Fragments arranged by size:
4545 960 99 73
BamHI G'GATC_C
Cuts at: 2374 2788 3007 3374 2374
Size: 414 219 367 4677
Fragments arranged by size:
4677 414 367 219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2826 2998 4453 618
Size: 325 217 627 387 652 172 1455 1842
Fragments arranged by size:
1842 1455 652 627 387 325 217 172
BanII G_rGCy'C
Cuts at: 1391 2996 1391
Size: 1605 4072
BbeI G_GCGC'C
Cuts at: 2178 2178
Size: 5677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 2263 1548
Size: 715 4962
BfrBI ATG'CAT
Cuts at: 2248 2248
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 3738 4894 1790
Size: 1948 1156 2573
Fragments arranged by size:
2573 1948 1156
BmgBI CAC'GTC
Cuts at: 1318 2115 1318
Size: 797 4880
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 2705 3408
Size: 416 53 83 399 24 663 625 703
Cuts at: 3408 4366 442
Size: 958 1753
Fragments arranged by size:
1753 958 703 663 625 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2463 4337 1239
Size: 997 227 1874 2579
Fragments arranged by size:
2579 1874 997 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 3802 4667 4965 2141
Size: 1661 865 298 2853
Fragments arranged by size:
2853 1661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2439 4340 882
Size: 617 940 1901 2219
Fragments arranged by size:
2219 1901 940 617
BsaAI yAC'GTr
Cuts at: 493 1204 2512 3468 493
Size: 711 1308 956 2702
Fragments arranged by size:
2702 1308 956 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 3484 3866 4501
Size: 53 83 186 936 642 1309 382 635
Cuts at: 4501 275
Size: 1451
Fragments arranged by size:
1451 1309 936 642 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2009 4110 4508 4782 4929 5340 2009
Size: 2101 398 274 147 411 2346
Fragments arranged by size:
2346 2101 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5452
Fragments arranged by size:
5452 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2092 1186
Size: 861 45 4771
Fragments arranged by size:
4771 861 45
BseYI C'CCAG_C
Cuts at: 2396 4880 5376 2396
Size: 2484 496 2697
Fragments arranged by size:
2697 2484 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2534 1163
Size: 573 798 4306
Fragments arranged by size:
4306 798 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
BsmBI CGTCTCn'nnnn_
Cuts at: 2129 2129
Size: 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2996 3738 3823 4894 1391
Size: 399 1206 742 85 1071 2174
Fragments arranged by size:
2174 1206 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2137 2171 2448 4321 4508 1700
Size: 110 199 128 34 277 1873 187 2869
Fragments arranged by size:
2869 1873 277 199 187 128 110 34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 2833 3737 4749 2833
Size: 904 1012 3761
Fragments arranged by size:
3761 1012 904
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2849 794
Size: 2055 3622
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 2374 2788 3007 3374 3759 3776 5228 5314
Size: 402 414 219 367 385 17 1452 86
Cuts at: 5314 5326 5672 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 414 402 385 367 346 219
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2640 2640
Size: 5677
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2824 487
Size: 683 56 1598 3340
Fragments arranged by size:
3340 1598 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3987 4007 3193
Size: 794 20 4863
Fragments arranged by size:
4863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 3124 4013
Size: 54 1425 50 275 18 309 985 889
Cuts at: 4013 5642 8
Size: 1629 43
Fragments arranged by size:
1629 1425 985 889 309 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3607 1353
Size: 2254 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2463 2839 2946 3740 4337 5124
Size: 393 604 227 376 107 794 597 787
Cuts at: 5124 5392 1239
Size: 268 1524
Fragments arranged by size:
1524 794 787 604 597 393 376 268
227 107
EcoO109I rG'GnC_Cy
Cuts at: 2283 2400 2283
Size: 117 5560
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2291 2680 2291
Size: 389 5288
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2178 4824 1678
Size: 500 2646 2531
Fragments arranged by size:
2646 2531 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2392 2424 4318
Size: 32 135 32 467 32 321 32 1894
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1894 467 321 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2174 2174
Size: 5677
KpnI G_GTAC'C
Cuts at: 2830 3002 2830
Size: 172 5505
MfeI C'AATT_G
Cuts at: 2237 3276 2237
Size: 1039 4638
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2228 4791 4975 1305
Size: 923 2563 184 2007
Fragments arranged by size:
2563 2007 923 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3855
Fragments arranged by size:
3855 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 3635 3994 4153 518
Size: 648 951 1518 359 159 2042
Fragments arranged by size:
2042 1518 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3093 3770 4918 5163 1606
Size: 1487 677 1148 245 2120
Fragments arranged by size:
2120 1487 1148 677 245
NaeI GCC'GGC
Cuts at: 2139 2139
Size: 5677
NarI GG'CG_CC
Cuts at: 2175 2175
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NgoMIV G'CCGG_C
Cuts at: 2137 2137
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NsiI A_TGCA'T
Cuts at: 2250 2250
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2213 3035 4580 1132
Size: 97 984 822 1545 2229
Fragments arranged by size:
2229 1545 984 822 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2283 2400 2283
Size: 117 5560
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2492 2524 2492
Size: 32 5645
PstI C_TGCA'G
Cuts at: 830 3029 830
Size: 2199 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SanDI GG'GwC_CC
Cuts at: 2283 2283
Size: 5677
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 3025 4160 4841 5032 826
Size: 219 1980 1135 681 191 1471
Fragments arranged by size:
1980 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2176 2176
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 2171 1700
Size: 471 5206
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 3781 4682 4944 820
Size: 197 926 213 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 213 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 2213 3035 1132
Size: 1081 822 3774
Fragments arranged by size:
3774 1081 822
SspI AAT'ATT
Cuts at: 5 52 2695 2782 3601 5
Size: 47 2643 87 819 2081
Fragments arranged by size:
2643 2081 819 87 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2973 3116 513
Size: 552 1908 143 3074
Fragments arranged by size:
3074 1908 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3704 3721 3874 4059 4504 325
Size: 1255 2124 17 153 185 445 1498
Fragments arranged by size:
2124 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3923 5635 96
Size: 274 80 33 51 1443 1946 1712 138
Fragments arranged by size:
1946 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2905 2937 5637 5669 2905
Size: 32 2700 32 2913
Fragments arranged by size:
2913 2700 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2211 2211
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI
PvuII RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI