Restriction map for
pFN21AB9359
(Circular) (Six-base) MAPSORT of: pFN21AB9359.seq Check: 1327 from: 1 to: 5581
pFN21AB9359 5581 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2730 3644 5028 5296 1632
Size: 1098 914 1384 268 1917
Fragments arranged by size:
1917 1384 1098 914 268
AfeI AGC'GCT
Cuts at: 2114 2219 2114
Size: 105 5476
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5384
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 2711 1166
Size: 1545 4036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5581
AlwNI CAG_nnn'CTG
Cuts at: 2095 2308 3324 4896 2095
Size: 213 1016 1572 2780
Fragments arranged by size:
2780 1572 1016 213
ApaI G_GGCC'C
Cuts at: 2838 2838
Size: 5581
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2890 3116 3268 5377 1493
Size: 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2814 2906 1870
Size: 73 100 771 92 4545
Fragments arranged by size:
4545 771 100 92 73
AvrII C'CTAG_G
Cuts at: 2437 2437
Size: 5581
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2651 2902 4357 618
Size: 325 217 627 240 624 251 1455 1842
Fragments arranged by size:
1842 1455 627 624 325 251 240 217
BanII G_rGCy'C
Cuts at: 1391 2679 2822 2838 2900 1391
Size: 1288 143 16 62 4072
Fragments arranged by size:
4072 1288 143 62 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 928
Size: 971 328 4282
Fragments arranged by size:
4282 971 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3772 3806 1903
Size: 34 1835 34 3678
Fragments arranged by size:
3678 1835 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4192 136
Size: 107 122 71 631 605 2520 1525
Fragments arranged by size:
2520 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5576 5576
Size: 5581
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 2838 3642 4798 1790
Size: 1048 804 1156 2573
Fragments arranged by size:
2573 1156 1048 804
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2431 3312 4270
Size: 416 53 83 399 24 1014 881 958
Cuts at: 4270 442
Size: 1753
Fragments arranged by size:
1753 1014 958 881 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2219 2776 2806 4241 1239
Size: 887 93 557 30 1435 2579
Fragments arranged by size:
2579 1435 887 557 93 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 3706 4571 4869 2432
Size: 1274 865 298 3144
Fragments arranged by size:
3144 1274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2342 4244 882
Size: 617 843 1902 2219
Fragments arranged by size:
2219 1902 843 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3372 493
Size: 711 915 1253 2702
Fragments arranged by size:
2702 1253 915 711
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3388 3770 4405 275
Size: 53 83 186 936 1855 382 635 1451
Fragments arranged by size:
1855 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4014 4412 4686 4833 5244 4014
Size: 398 274 147 411 4351
Fragments arranged by size:
4351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2836 2866 1178
Size: 30 165 30 597 30 806 30 3893
Fragments arranged by size:
3893 806 597 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2225 2240 1186
Size: 937 102 15 4527
Fragments arranged by size:
4527 937 102 15
BseYI C'CCAG_C
Cuts at: 2068 2797 2837 4784 5280 2068
Size: 729 40 1947 496 2369
Fragments arranged by size:
2369 1947 729 496 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 2483 2736 1163
Size: 573 432 315 253 4008
Fragments arranged by size:
4008 573 432 315 253
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3031 3792 4820 1540
Size: 275 186 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2679 3642 3727 4798 2679
Size: 963 85 1071 3462
Fragments arranged by size:
3462 1071 963 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2679 2822 2838 2900 3642 3727 4798
Size: 399 889 143 16 62 742 85 1071
Cuts at: 4798 1391
Size: 2174
Fragments arranged by size:
2174 1071 889 742 399 143 85 62
16
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2936 844
Size: 2092 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2780 3468 1394
Size: 429 957 688 3507
Fragments arranged by size:
3507 957 688 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4076 4250 65
Size: 1183 2828 174 1396
Fragments arranged by size:
2828 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2502 4225 4412 1700
Size: 110 692 1723 187 2869
Fragments arranged by size:
2869 1723 692 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 794
Size: 1301 4280
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstXI CCAn_nnnn'nTGG
Cuts at: 2878 2878
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2911 3278 3663 3680 5132 5218 5230 5576
Size: 939 367 385 17 1452 86 12 346
Cuts at: 5576 1972
Size: 1977
Fragments arranged by size:
1977 1452 939 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2768 2768
Size: 5581
BtgI C'CryG_G
Cuts at: 513 1065 2004 2060 2616 513
Size: 552 939 56 556 3478
Fragments arranged by size:
3478 939 556 552 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4842
Fragments arranged by size:
4842 683 56
BtsI GCAGTG_nn'
Cuts at: 2643 3097 3891 3911 2643
Size: 454 794 20 4313
Fragments arranged by size:
4313 794 454 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2081 2396 4588 809
Size: 507 765 315 2192 1802
Fragments arranged by size:
2192 1802 765 507 315
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 3028 3917
Size: 54 1425 50 275 18 414 784 889
Cuts at: 3917 5546 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 784 414 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3511 1353
Size: 2158 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 4168 4542 4688 387
Size: 1064 138 501 2078 374 146 1280
Fragments arranged by size:
2078 1280 1064 501 374 146 138
EcoICRI GAG'CTC
Cuts at: 2677 2677
Size: 5581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2219 2730 2776 2806 3644 4241
Size: 393 494 93 511 46 30 838 597
Cuts at: 4241 5028 5296 1239
Size: 787 268 1524
Fragments arranged by size:
1524 838 787 597 511 494 393 268
93 46 30
EcoO109I rG'GnC_Cy
Cuts at: 2455 2834 2455
Size: 379 5202
EcoRI G'AATT_C
Cuts at: 2890 3268 2890
Size: 378 5203
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 2116 2221 2804 4728 1678
Size: 438 105 583 1924 2531
Fragments arranged by size:
2531 1924 583 438 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2836 2868 4222 4254 4296
Size: 32 135 32 1264 32 1354 32 42
Cuts at: 4296 4328 1373
Size: 32 2626
Fragments arranged by size:
2626 1354 1264 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2925 3171 669
Size: 988 281 987 246 3079
Fragments arranged by size:
3079 988 987 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4695 4879 1305
Size: 3390 184 2007
Fragments arranged by size:
3390 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 10
Size: 54 1425 343 414 3345
Fragments arranged by size:
3345 1425 414 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2711 3539 3898 4057 518
Size: 648 1545 828 359 159 2042
Fragments arranged by size:
2042 1545 828 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2152 2997 3674 4822 5067 1606
Size: 456 90 845 677 1148 245 2120
Fragments arranged by size:
2120 1148 845 677 456 245 90
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4090
Fragments arranged by size:
4090 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2173 2326 2549 2939 4484 1132
Size: 97 944 153 223 390 1545 2229
Fragments arranged by size:
2229 1545 944 390 223 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5581
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2455 2455
Size: 5581
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PspOMI G'GGCC_C
Cuts at: 2834 2834
Size: 5581
PstI C_TGCA'G
Cuts at: 830 2091 2638 2933 830
Size: 1261 547 295 3478
Fragments arranged by size:
3478 1261 547 295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
SacI G_AGCT'C
Cuts at: 2679 2679
Size: 5581
SacII CC_GC'GG
Cuts at: 2063 2063
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 3829 5541 3829
Size: 1712 3869
SexAI A'CCwGG_T
Cuts at: 2877 2877
Size: 5581
SfcI C'TryA_G
Cuts at: 826 1045 2087 2574 2634 2856 2929 4064 4745
Size: 219 1042 487 60 222 73 1135 681
Cuts at: 4745 4936 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1042 681 487 222 219 191
73 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2045 2816 2908 1872
Size: 173 771 92 4545
Fragments arranged by size:
4545 771 173 92
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 3685 4586 4848 820
Size: 197 926 504 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 504 262 197
SnaBI TAC'GTA
Cuts at: 493 2119 3372 493
Size: 1626 1253 2702
Fragments arranged by size:
2702 1626 1253
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2326 2549 2939 1132
Size: 1194 223 390 3774
Fragments arranged by size:
3774 1194 390 223
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 2531 2646 1517
Size: 1014 115 4452
Fragments arranged by size:
4452 1014 115
StyI C'CwwG_G
Cuts at: 513 1065 2004 2437 2532 2830 3020 513
Size: 552 939 433 95 298 190 3074
Fragments arranged by size:
3074 939 552 433 298 190 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 2538 2639 3608 3625 3778 3963
Size: 1255 720 238 101 969 17 153 185
Cuts at: 3963 4408 325
Size: 445 1498
Fragments arranged by size:
1498 1255 969 720 445 238 185 153
101 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3827 5539 96
Size: 274 80 33 51 1443 1850 1712 138
Fragments arranged by size:
1850 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2466 2498 5541 5573 2000
Size: 32 434 32 3043 32 2008
Fragments arranged by size:
3043 2008 434 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5581
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2254
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2043 2814 2906 1870
Size: 173 771 92 4545
Fragments arranged by size:
4545 771 173 92
XmnI GAAnn'nnTTC
Cuts at: 3710 3710
Size: 5581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SanDI
SapI SfiI SfoI SrfI SwaI