Restriction map for
pFN21AB9348
(Circular) (Six-base) MAPSORT of: pFN21AB9348.seq Check: 6387 from: 1 to: 5524
pFN21AB9348 5524 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2845 2845
Size: 5524
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 3587 4971 5239 1632
Size: 941 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1014 941 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 4423 5400 1203
Size: 3220 977 1327
Fragments arranged by size:
3220 1327 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 2421 4253 2421
Size: 1832 3692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2553 1184
Size: 1369 4155
AlwNI CAG_nnn'CTG
Cuts at: 3267 4839 3267
Size: 1572 3952
ApaI G_GGCC'C
Cuts at: 2820 2820
Size: 5524
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2849 1870
Size: 73 906 4545
Fragments arranged by size:
4545 906 73
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2676 2845 4300 618
Size: 325 217 627 889 169 1455 1842
Fragments arranged by size:
1842 1455 889 627 325 217 169
BanII G_rGCy'C
Cuts at: 1391 2514 2820 2843 1391
Size: 1123 306 23 4072
Fragments arranged by size:
4072 1123 306 23
BbeI G_GCGC'C
Cuts at: 2680 2680
Size: 5524
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4553
BbvCI CC'TCA_GC
Cuts at: 2541 2541
Size: 5524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2770 2804 3715 3749 1903
Size: 34 833 34 911 34 3678
Fragments arranged by size:
3678 911 833 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 2568 1548
Size: 1020 4504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4135 136
Size: 107 122 71 631 605 2463 1525
Fragments arranged by size:
2463 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 2820 3585 4741 1790
Size: 1030 765 1156 2573
Fragments arranged by size:
2573 1156 1030 765
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2415 3255 4213
Size: 416 53 83 399 24 998 840 958
Cuts at: 4213 442
Size: 1753
Fragments arranged by size:
1753 998 958 840 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2433 2433
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2117 2222 2252 2420 2476 2738 4184
Size: 818 60 105 30 168 56 262 1446
Cuts at: 4184 1239
Size: 2579
Fragments arranged by size:
2579 1446 818 262 168 105 60 56
30
Bpu10I CC'TnA_GC
Cuts at: 2541 2541
Size: 5524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2009 3649 4514 4812 2009
Size: 1640 865 298 2721
Fragments arranged by size:
2721 1640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2798 4187 882
Size: 617 1299 1389 2219
Fragments arranged by size:
2219 1389 1299 617
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 2699 5518 2699
Size: 2819 2705
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2677 3331 3713 4348
Size: 53 83 186 936 1144 654 382 635
Cuts at: 4348 275
Size: 1451
Fragments arranged by size:
1451 1144 936 654 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2467 3957 4355 4629 4776 5187 2467
Size: 1490 398 274 147 411 2804
Fragments arranged by size:
2804 1490 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5299
Fragments arranged by size:
5299 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2335 2558 1186
Size: 928 221 223 4152
Fragments arranged by size:
4152 928 223 221
BseYI C'CCAG_C
Cuts at: 2559 4727 5223 2559
Size: 2168 496 2860
Fragments arranged by size:
2860 2168 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4951
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2974 3735 4763 1540
Size: 275 186 973 761 1028 2301
Fragments arranged by size:
2301 1028 973 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2490 2514 3585 3670 4741 2043
Size: 447 24 1071 85 1071 2826
Fragments arranged by size:
2826 1071 1071 447 85 24
BsmI GAATG_Cn'
Cuts at: 3035 3128 3035
Size: 93 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2490 2514 2820 2843 3585 3670
Size: 399 253 447 24 306 23 742 85
Cuts at: 3670 4741 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 306 253 85
24 23
BspEI T'CCGG_A
Cuts at: 2467 5187 2467
Size: 2720 2804
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2283 2879 844
Size: 1439 596 3489
Fragments arranged by size:
3489 1439 596
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4168 4355 1700
Size: 110 2358 187 2869
Fragments arranged by size:
2869 2358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 3584 4596 3584
Size: 1012 4512
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2047 794
Size: 1253 4271
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2160 2263 2443 2128
Size: 32 103 180 5209
Fragments arranged by size:
5209 180 103 32
BstYI r'GATC_y
Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519
Size: 882 367 385 17 1452 86 12 346
Cuts at: 5519 1972
Size: 1977
Fragments arranged by size:
1977 1452 882 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2016 2016
Size: 5524
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 513
Size: 552 939 123 3910
Fragments arranged by size:
3910 939 552 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 2349 3040 3834 3854 2349
Size: 691 794 20 4019
Fragments arranged by size:
4019 794 691 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2791 4531 809
Size: 507 1475 1740 1802
Fragments arranged by size:
1802 1740 1475 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 2751 2971
Size: 54 1425 50 275 18 224 697 220
Cuts at: 2971 3860 5489 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 697 275 224 220 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2971 1537
Size: 275 1159 4090
Fragments arranged by size:
4090 1159 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 2326 2519 3454 1353
Size: 704 269 193 935 3423
Fragments arranged by size:
3423 935 704 269 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2512 2512
Size: 5524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2117 2222 2252 2420 2476 2573
Size: 393 425 60 105 30 168 56 97
Cuts at: 2573 2738 3587 4184 4971 5239 1239
Size: 165 849 597 787 268 1524
Fragments arranged by size:
1524 849 787 597 425 393 268 168
165 105 97 60 56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2720 2402
Size: 318 5206
EcoO109I rG'GnC_Cy
Cuts at: 2020 2548 2817 2020
Size: 528 269 4727
Fragments arranged by size:
4727 528 269
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 5524
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 2680 4671 1678
Size: 1002 1991 2531
Fragments arranged by size:
2531 1991 1002
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 2407 2439 4165
Size: 32 135 32 602 32 201 32 1726
Cuts at: 4165 4197 4239 4271 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1726 602 201 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3114 669
Size: 988 281 930 246 3079
Fragments arranged by size:
3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KasI G'GCGC_C
Cuts at: 2676 2676
Size: 5524
KpnI G_GTAC'C
Cuts at: 2849 2849
Size: 5524
MfeI C'AATT_G
Cuts at: 2437 3123 2437
Size: 686 4838
MluI A'CGCG_T
Cuts at: 5400 5400
Size: 5524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4822 1305
Size: 3333 184 2007
Fragments arranged by size:
3333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2056 10
Size: 54 1425 343 224 3478
Fragments arranged by size:
3478 1425 343 224 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3482 3841 4000 518
Size: 648 843 1473 359 159 2042
Fragments arranged by size:
2042 1473 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2940 3617 4765 5010 1606
Size: 1334 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 677 245
NarI GG'CG_CC
Cuts at: 2677 2677
Size: 5524
NcoI C'CATG_G
Cuts at: 513 1065 2004 2127 513
Size: 552 939 123 3910
Fragments arranged by size:
3910 939 552 123
NdeI CA'TA_TG
Cuts at: 387 2179 387
Size: 1792 3732
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2014 2882 4427 1132
Size: 97 785 868 1545 2229
Fragments arranged by size:
2229 1545 868 785 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2183 1108
Size: 1075 4449
PfoI T'CCnGG_A
Cuts at: 2715 2715
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2548 2020
Size: 528 4996
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PspOMI G'GGCC_C
Cuts at: 2816 2816
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2876 830
Size: 2046 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
SacI G_AGCT'C
Cuts at: 2514 2514
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SapI GCTCTTCn'nnn_
Cuts at: 2326 2519 2326
Size: 193 5331
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 2621 3772 5484 2621
Size: 1151 1712 2661
Fragments arranged by size:
2661 1712 1151
SexAI A'CCwGG_T
Cuts at: 2481 2481
Size: 5524
SfcI C'TryA_G
Cuts at: 826 1045 2286 2872 4007 4688 4879 826
Size: 219 1241 586 1135 681 191 1471
Fragments arranged by size:
1471 1241 1135 681 586 219 191
SfoI GGC'GCC
Cuts at: 2678 2678
Size: 5524
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2851 1872
Size: 979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2024 3628 4529 4791 820
Size: 197 926 81 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197 81
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5524
StyI C'CwwG_G
Cuts at: 513 1065 2004 2051 2127 2820 2963 513
Size: 552 939 47 76 693 143 3074
Fragments arranged by size:
3074 939 693 552 143 76 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 2273 3551 3568 3721 3906 4351
Size: 1255 590 103 1278 17 153 185 445
Cuts at: 4351 325
Size: 1498
Fragments arranged by size:
1498 1278 1255 590 445 185 153 103
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2205 2619 3770
Size: 274 80 33 51 1443 228 414 1151
Cuts at: 3770 5482 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1151 414 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2849 1870
Size: 979 4545
XmnI GAAnn'nnTTC
Cuts at: 3653 3653
Size: 5524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacII SanDI SfiI SrfI SwaI XcmI