Restriction map for
pFN21AB9292
(Circular) (Six-base) MAPSORT of: pFN21AB9292.seq Check: 209 from: 1 to: 5515
pFN21AB9292 5515 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AfeI AGC'GCT
Cuts at: 2045 2045
Size: 5515
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 2188 4244 2188
Size: 2056 3459
AleI CACnn'nnGTG
Cuts at: 1166 2227 1166
Size: 1061 4454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2245 2487 2571 1184
Size: 1061 242 84 4128
Fragments arranged by size:
4128 1061 242 84
AlwNI CAG_nnn'CTG
Cuts at: 3258 4830 3258
Size: 1572 3943
ApaI G_GGCC'C
Cuts at: 2165 2219 2305 2165
Size: 54 86 5375
Fragments arranged by size:
5375 86 54
ApaLI G'TGCA_C
Cuts at: 2633 3572 4728 2633
Size: 939 1156 3420
Fragments arranged by size:
3420 1156 939
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2840 1870
Size: 73 68 829 4545
Fragments arranged by size:
4545 829 73 68
AvrII C'CTAG_G
Cuts at: 2107 2107
Size: 5515
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2836 4291 618
Size: 325 217 627 420 629 1455 1842
Fragments arranged by size:
1842 1455 629 627 420 325 217
BanII G_rGCy'C
Cuts at: 1391 2165 2219 2305 2719 2834 1391
Size: 774 54 86 414 115 4072
Fragments arranged by size:
4072 774 414 115 86 54
BbeI G_GCGC'C
Cuts at: 2211 2211
Size: 5515
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2609 2680 928
Size: 971 710 71 3763
Fragments arranged by size:
3763 971 710 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2169 4126 136
Size: 107 122 71 631 605 497 1957 1525
Fragments arranged by size:
1957 1525 631 605 497 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2165 2219 2305 2637 3576 4732 1790
Size: 375 54 86 332 939 1156 2573
Fragments arranged by size:
2573 1156 939 375 332 86 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2167 3246 4204
Size: 416 53 83 399 24 750 1079 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 1079 958 750 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 4175 1239
Size: 905 2031 2579
Fragments arranged by size:
2579 2031 905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2189 2208 3322 3704
Size: 53 83 186 936 656 19 1114 382
Cuts at: 3704 4339 275
Size: 635 1451
Fragments arranged by size:
1451 1114 936 656 635 382 186 83
53 19
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5290
Fragments arranged by size:
5290 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2164 2291 2324 2752 1186
Size: 895 83 127 33 428 3949
Fragments arranged by size:
3949 895 428 127 83 33
BseYI C'CCAG_C
Cuts at: 2061 2568 4718 5214 2061
Size: 507 2150 496 2362
Fragments arranged by size:
2362 2150 507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 1163
Size: 573 345 4597
Fragments arranged by size:
4597 573 345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2169 2965 3726 4754 1540
Size: 275 186 168 796 761 1028 2301
Fragments arranged by size:
2301 1028 796 761 275 186 168
BsiHKAI G_wGCw'C
Cuts at: 2637 2719 3576 3661 4732 2637
Size: 82 857 85 1071 3420
Fragments arranged by size:
3420 1071 857 85 82
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2219 2305 2637 2719 2834 3576
Size: 399 375 54 86 332 82 115 742
Cuts at: 3576 3661 4732 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 332 115 86
85 82 54
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 2644 3404 5134 2644
Size: 760 1730 3025
Fragments arranged by size:
3025 1730 760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 2627 3402 1394
Size: 429 196 608 775 3507
Fragments arranged by size:
3507 775 608 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2115 2168 2263 4159 4346 1700
Size: 110 305 53 95 1896 187 2869
Fragments arranged by size:
2869 1896 305 187 110 95 53
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 2294 1925
Size: 369 5146
BssSI C'ACGA_G
Cuts at: 2636 3575 4587 2636
Size: 939 1012 3564
Fragments arranged by size:
3564 1012 939
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2774 513
Size: 552 1709 3254
Fragments arranged by size:
3254 1709 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 3031 3825 3845 2372
Size: 659 794 20 4042
Fragments arranged by size:
4042 794 659 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DraIII CAC_nnn'GTG
Cuts at: 2725 2725
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2962 3851
Size: 54 1425 50 275 18 336 796 889
Cuts at: 3851 5480 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 796 336 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2166 2962 1537
Size: 275 354 796 4090
Fragments arranged by size:
4090 796 354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 3445 1353
Size: 668 1424 3423
Fragments arranged by size:
3423 1424 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2297 4102 4476 4622 387
Size: 1064 138 708 1805 374 146 1280
Fragments arranged by size:
1805 1280 1064 708 374 146 138
EcoICRI GAG'CTC
Cuts at: 2717 2717
Size: 5515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 3578 4175 4962 5230 1239
Size: 393 512 1434 597 787 268 1524
Fragments arranged by size:
1524 1434 787 597 512 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2161 2215 2216 2301 2302 2161
Size: 54 1 85 1 5374
Fragments arranged by size:
5374 85 54 1 1
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2047 2211 4662 1678
Size: 369 164 2451 2531
Fragments arranged by size:
2531 2451 369 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 4156 4188 4230
Size: 32 135 32 602 32 1950 32 42
Cuts at: 4230 4262 1373
Size: 32 2626
Fragments arranged by size:
2626 1950 602 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KasI G'GCGC_C
Cuts at: 2207 2207
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2227 3473 3832 3991 518
Size: 648 1061 1246 359 159 2042
Fragments arranged by size:
2042 1246 1061 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2931 3608 4756 5001 1606
Size: 1325 677 1148 245 2120
Fragments arranged by size:
2120 1325 1148 677 245
NaeI GCC'GGC
Cuts at: 2117 2170 2265 2117
Size: 53 95 5367
Fragments arranged by size:
5367 95 53
NarI GG'CG_CC
Cuts at: 2208 2208
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 2774 513
Size: 552 1709 3254
Fragments arranged by size:
3254 1709 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NgoMIV G'CCGG_C
Cuts at: 2115 2168 2263 2115
Size: 53 95 5367
Fragments arranged by size:
5367 95 53
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2161 2215 2301 2161
Size: 54 86 5375
Fragments arranged by size:
5375 86 54
PstI C_TGCA'G
Cuts at: 830 2867 830
Size: 2037 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
SacI G_AGCT'C
Cuts at: 2719 2719
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SapI GCTCTTCn'nnn_
Cuts at: 2021 2021
Size: 5515
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 2449 3763 5475 2449
Size: 1314 1712 2489
Fragments arranged by size:
2489 1712 1314
SfcI C'TryA_G
Cuts at: 826 1045 2863 3998 4679 4870 826
Size: 219 1818 1135 681 191 1471
Fragments arranged by size:
1818 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2209 2209
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2013 2842 1872
Size: 141 829 4545
Fragments arranged by size:
4545 829 141
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2107 2774 2954 513
Size: 552 1042 667 180 3074
Fragments arranged by size:
3074 1042 667 552 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2455 2600 3542 3559 3712 3897 4342
Size: 1255 875 145 942 17 153 185 445
Cuts at: 4342 325
Size: 1498
Fragments arranged by size:
1498 1255 942 875 445 185 153 145
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2034 2447 3761
Size: 274 80 33 51 1443 57 413 1314
Cuts at: 3761 5473 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1314 413 274 138 80 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2011 2840 1870
Size: 141 829 4545
Fragments arranged by size:
4545 829 141
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI