Restriction map for
pFN21AB9288
(Circular) (Six-base) MAPSORT of: pFN21AB9288.seq Check: 8970 from: 1 to: 7798
pFN21AB9288 7798 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2007 2007
Size: 7798
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5608 278
Size: 53 83 186 936 4072 2468
Fragments arranged by size:
4072 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5141 1937
Size: 3204 4594
Acc65I G'GTAC_C
Cuts at: 5119 5119
Size: 7798
AclI AA'CG_TT
Cuts at: 1563 4519 5925 6298 1563
Size: 2956 1406 373 3063
Fragments arranged by size:
3063 2956 1406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 2161 2187 3189 3250 3319 3385 3973
Size: 425 104 26 1002 61 69 66 588
Cuts at: 3973 4384 5032 5861 7245 7513 1632
Size: 411 648 829 1384 268 1917
Fragments arranged by size:
1917 1384 1002 829 648 588 425 411
268 104 69 66 61 26
AfeI AGC'GCT
Cuts at: 4604 4604
Size: 7798
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7601
AflIII A'CryG_T
Cuts at: 1203 3202 3384 4553 6697 7674 1203
Size: 1999 182 1169 2144 977 1327
Fragments arranged by size:
2144 1999 1327 1169 977 182
AgeI A'CCGG_T
Cuts at: 6629 6629
Size: 7798
AhdI GACnn_n'nnGTC
Cuts at: 3486 6527 3486
Size: 3041 4757
AleI CACnn'nnGTG
Cuts at: 1166 3423 3614 4116 1166
Size: 2257 191 502 4848
Fragments arranged by size:
4848 2257 502 191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7798
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2801 2833 4232 4264 2801
Size: 32 1399 32 6335
Fragments arranged by size:
6335 1399 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2161 2314 2924 3471 5541 7113 2161
Size: 153 610 547 2070 1572 2846
Fragments arranged by size:
2846 2070 1572 610 547 153
ApaI G_GGCC'C
Cuts at: 2211 2444 2211
Size: 233 7565
ApaLI G'TGCA_C
Cuts at: 5855 7011 5855
Size: 1156 6642
ApoI r'AATT_y
Cuts at: 1493 2963 3351 3603 3704 3956 4107 5107 5333
Size: 1470 388 252 101 252 151 1000 226
Cuts at: 5333 5485 7594 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1470 1000 388 252 252 226
152 151 101
AseI AT'TA_AT
Cuts at: 160 1032 6352 160
Size: 872 5320 1606
Fragments arranged by size:
5320 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7798
AvaI C'yCGr_G
Cuts at: 1870 1943 2437 2635 2650 5007 5123 1870
Size: 73 494 198 15 2357 116 4545
Fragments arranged by size:
4545 2357 494 198 116 73 15
BamHI G'GATC_C
Cuts at: 4668 5128 5495 4668
Size: 460 367 6971
Fragments arranged by size:
6971 460 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2646 5119 6574 618
Size: 325 217 627 859 2473 1455 1842
Fragments arranged by size:
2473 1842 1455 859 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2211 2337 2444 2456 2485 3057 3315 4287
Size: 820 126 107 12 29 572 258 972
Cuts at: 4287 4892 5117 1391
Size: 605 225 4072
Fragments arranged by size:
4072 972 820 605 572 258 225 126
107 29 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2043 2193 3428 4791 4869 5049 928
Size: 971 144 150 1235 1363 78 180 3677
Fragments arranged by size:
3677 1363 1235 971 180 150 144 78
BbvCI CC'TCA_GC
Cuts at: 2255 2255
Size: 7798
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2591 2625 5989 6023 1903
Size: 34 50 34 570 34 3364 34 3678
Fragments arranged by size:
3678 3364 570 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5690 6900 5690
Size: 1210 6588
BclI T'GATC_A
Cuts at: 1548 3757 1548
Size: 2209 5589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4305 6409 136
Size: 107 122 71 631 605 2633 2104 1525
Fragments arranged by size:
2633 2104 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7793 7793
Size: 7798
BlpI GC'TnA_GC
Cuts at: 2901 5215 2901
Size: 2314 5484
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2444 2651 5859 7015 1790
Size: 421 233 207 3208 1156 2573
Fragments arranged by size:
3208 2573 1156 421 233 207
BmgBI CAC'GTC
Cuts at: 1318 3385 1318
Size: 2067 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 5529 6487
Size: 416 53 83 399 24 624 3488 958
Cuts at: 6487 442
Size: 1753
Fragments arranged by size:
3488 1753 958 624 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7798
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2960
Size: 7798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1995 2097 2414 2449 2573 2678 2816 2930
Size: 756 102 317 35 124 105 138 114
Cuts at: 2930 3633 4380 4876 4892 6458 1239
Size: 703 747 496 16 1566 2579
Fragments arranged by size:
2579 1566 756 747 703 496 317 138
124 114 105 102 35 16
Bpu10I CC'TnA_GC
Cuts at: 2040 2255 2315 3004 3407 3748 2040
Size: 215 60 689 403 341 6090
Fragments arranged by size:
6090 689 403 341 215 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3513 5923 6788 7086 3513
Size: 2410 865 298 4225
Fragments arranged by size:
4225 2410 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2649 2693 3128 3765 4678 6461 882
Size: 617 1150 44 435 637 913 1783 2219
Fragments arranged by size:
2219 1783 1150 913 637 617 435 44
BsaAI yAC'GTr
Cuts at: 493 1204 2579 4556 5589 493
Size: 711 1375 1977 1033 2702
Fragments arranged by size:
2702 1977 1375 1033 711
BsaBI GATnn'nnATC
Cuts at: 3913 7792 3913
Size: 3879 3919
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 5605 5987 6622
Size: 53 83 186 936 2125 1947 382 635
Cuts at: 6622 275
Size: 1451
Fragments arranged by size:
2125 1947 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3570 6231 6629 6903 7050 7461 3570
Size: 2661 398 274 147 411 3907
Fragments arranged by size:
3907 2661 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 2646 2676 1178
Size: 30 165 30 852 30 361 30 6300
Fragments arranged by size:
6300 852 361 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2411 3962 4295 4325 1186
Size: 1204 21 1551 333 30 4659
Fragments arranged by size:
4659 1551 1204 333 30 21
BseYI C'CCAG_C
Cuts at: 2591 2688 7001 7497 2591
Size: 97 4313 496 2892
Fragments arranged by size:
4313 2892 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2369 2478 3484 3662 3829 4399
Size: 573 402 231 109 1006 178 167 570
Cuts at: 4399 4776 1163
Size: 377 4185
Fragments arranged by size:
4185 1006 573 570 402 377 231 178
167 109
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5248 6009 7037 1540
Size: 275 186 3247 761 1028 2301
Fragments arranged by size:
3247 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 4027 4892 5859 5944 7015 2337
Size: 1690 865 967 85 1071 3120
Fragments arranged by size:
3120 1690 1071 967 865 85
BsmI GAATG_Cn'
Cuts at: 5309 5402 5309
Size: 93 7705
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2337 2444 2456 2485 2651 3057
Size: 399 421 126 107 12 29 166 406
Cuts at: 3057 3315 4027 4287 4892 5117 5859 5944 7015
Size: 258 712 260 605 225 742 85 1071
Cuts at: 7015 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 712 605 421 406 399
260 258 225 166 126 107 85 29
12
BspEI T'CCGG_A
Cuts at: 7461 7461
Size: 7798
BspHI T'CATG_A
Cuts at: 5687 7417 5687
Size: 1730 6068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2007 2851 5153 844
Size: 1163 844 2302 3489
Fragments arranged by size:
3489 2302 1163 844
BsrBI CCG'CTC
Cuts at: 1394 1823 5685 1394
Size: 429 3862 3507
Fragments arranged by size:
3862 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6293 6467 65
Size: 1183 5045 174 1396
Fragments arranged by size:
5045 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 6442 6629 1700
Size: 110 416 4216 187 2869
Fragments arranged by size:
4216 2869 416 187 110
BsrGI T'GTAC_A
Cuts at: 96 2778 96
Size: 2682 5116
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7798
BssSI C'ACGA_G
Cuts at: 5858 6870 5858
Size: 1012 6786
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3807 794
Size: 3013 4785
BstBI TT'CG_AA
Cuts at: 5060 6602 5060
Size: 1542 6256
BstEII G'GTnAC_C
Cuts at: 1184 2725 1184
Size: 1541 6257
BstXI CCAn_nnnn'nTGG
Cuts at: 2019 2019
Size: 7798
BstYI r'GATC_y
Cuts at: 1972 2283 4668 5128 5495 5880 5897 7349 7435
Size: 311 2385 460 367 385 17 1452 86
Cuts at: 7435 7447 7793 1972
Size: 12 346 1977
Fragments arranged by size:
2385 1977 1452 460 385 367 346 311
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2907 4215 2907
Size: 1308 6490
BtgI C'CryG_G
Cuts at: 513 1065 2876 513
Size: 552 1811 5435
Fragments arranged by size:
5435 1811 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7059
Fragments arranged by size:
7059 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4064 5314 6108 6128 3472
Size: 592 1250 794 20 5142
Fragments arranged by size:
5142 1250 794 592 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7798
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7763
DraI TTT'AAA
Cuts at: 3544 5102 3544
Size: 1558 6240
DraIII CAC_nnn'GTG
Cuts at: 3445 3445
Size: 7798
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6805 809
Size: 507 5489 1802
Fragments arranged by size:
5489 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2152 5245 6134
Size: 54 1425 50 275 18 322 3093 889
Cuts at: 6134 7763 8
Size: 1629 43
Fragments arranged by size:
3093 1629 1425 889 322 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5245 1537
Size: 275 3433 4090
Fragments arranged by size:
4090 3433 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2180 3071 3126 4449 4842 5728 1353
Size: 735 92 891 55 1323 393 886 3423
Fragments arranged by size:
3423 1323 891 886 735 393 92 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2156 6385 6759 6905 387
Size: 1064 138 567 4229 374 146 1280
Fragments arranged by size:
4229 1280 1064 567 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 4890 2335
Size: 2555 5243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2057 2097 2161 2187 2414 2449
Size: 393 363 62 40 64 26 227 35
Cuts at: 2449 2573 2678 2816 2930 3189 3250 3319 3385
Size: 124 105 138 114 259 61 69 66
Cuts at: 3385 3633 3973 4380 4384 4876 4892 5032 5861
Size: 248 340 407 4 492 16 140 829
Cuts at: 5861 6458 7245 7513 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 829 787 597 492 407 393 363
340 268 259 248 227 140 138 124
114 105 69 66 64 62 61 40
35 26 16 4
EcoO109I rG'GnC_Cy
Cuts at: 2207 2208 2410 2434 2441 2676 2881 5016 2207
Size: 1 202 24 7 235 205 2135 4989
Fragments arranged by size:
4989 2135 235 205 202 24 7 1
EcoRI G'AATT_C
Cuts at: 3351 4107 5107 5485 3351
Size: 756 1000 378 5664
Fragments arranged by size:
5664 1000 756 378
FspI TGC'GCA
Cuts at: 6304 6304
Size: 7798
HaeII r_GCGC'y
Cuts at: 1678 4606 6945 1678
Size: 2928 2339 2531
Fragments arranged by size:
2928 2531 2339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2408 2440 3472
Size: 32 135 32 683 32 121 32 1032
Cuts at: 3472 3504 4399 4426 4431 4458 6439 6471 6513
Size: 32 895 27 5 27 1981 32 42
Cuts at: 6513 6545 1373
Size: 32 2626
Fragments arranged by size:
2626 1981 1032 895 683 135 121 42
32 32 32 32 32 32 32 27
27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4096 4657 5142 5388 669
Size: 988 281 2158 561 485 246 3079
Fragments arranged by size:
3079 2158 988 561 485 281 246
HindIII A'AGCT_T
Cuts at: 748 3648 4875 748
Size: 2900 1227 3671
Fragments arranged by size:
3671 2900 1227
HpaI GTT'AAC
Cuts at: 4657 5388 4657
Size: 731 7067
KpnI G_GTAC'C
Cuts at: 5123 5123
Size: 7798
MfeI C'AATT_G
Cuts at: 5397 5397
Size: 7798
MluI A'CGCG_T
Cuts at: 7674 7674
Size: 7798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2841 3606 3679 6912 7096 1305
Size: 1536 765 73 3233 184 2007
Fragments arranged by size:
3233 2007 1536 765 184 73
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2154 10
Size: 54 1425 343 322 5654
Fragments arranged by size:
5654 1425 343 322 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2500 3019 3252 3285 3423 3460 3544
Size: 648 1334 519 233 33 138 37 84
Cuts at: 3544 3614 4048 4116 4375 4470 5756 6115 6274
Size: 70 434 68 259 95 1286 359 159
Cuts at: 6274 518
Size: 2042
Fragments arranged by size:
2042 1334 1286 648 519 434 359 259
233 159 138 95 84 70 68 37
33
MspA1I CmG'CkG
Cuts at: 1606 4031 5214 5891 7039 7284 1606
Size: 2425 1183 677 1148 245 2120
Fragments arranged by size:
2425 2120 1183 1148 677 245
NaeI GCC'GGC
Cuts at: 2228 2228
Size: 7798
NcoI C'CATG_G
Cuts at: 513 1065 2876 513
Size: 552 1811 5435
Fragments arranged by size:
5435 1811 552
NdeI CA'TA_TG
Cuts at: 387 3835 3933 4949 5076 387
Size: 3448 98 1016 127 3109
Fragments arranged by size:
3448 3109 1016 127 98
NgoMIV G'CCGG_C
Cuts at: 2226 2226
Size: 7798
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7798
NotI GC'GGCC_GC
Cuts at: 5245 5245
Size: 7798
NspI r_CATG'y
Cuts at: 1132 1229 2027 2893 3206 4199 5156 6701 1132
Size: 97 798 866 313 993 957 1545 2229
Fragments arranged by size:
2229 1545 993 957 866 798 313 97
PasI CC'CwG_GG
Cuts at: 1348 4018 1348
Size: 2670 5128
PciI A'CATG_T
Cuts at: 3202 6697 3202
Size: 3495 4303
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7798
PfoI T'CCnGG_A
Cuts at: 2232 2426 2694 4908 2232
Size: 194 268 2214 5122
Fragments arranged by size:
5122 2214 268 194
PmeI GTTT'AAAC
Cuts at: 5102 5102
Size: 7798
PmlI CAC'GTG
Cuts at: 2579 2579
Size: 7798
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5867 5899 7380 7412 5867
Size: 32 1481 32 6253
Fragments arranged by size:
6253 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2434 2676 2410
Size: 24 242 7532
Fragments arranged by size:
7532 242 24
PshAI GACnn'nnGTC
Cuts at: 1613 3661 4008 1613
Size: 2048 347 5403
Fragments arranged by size:
5403 2048 347
PsiI TTA'TAA
Cuts at: 3627 5368 3627
Size: 1741 6057
PspOMI G'GGCC_C
Cuts at: 2207 2440 2207
Size: 233 7565
PstI C_TGCA'G
Cuts at: 830 2406 3803 4010 5150 830
Size: 1576 1397 207 1140 3478
Fragments arranged by size:
3478 1576 1397 1140 207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7798
SacI G_AGCT'C
Cuts at: 2337 4892 2337
Size: 2555 5243
SalI G'TCGA_C
Cuts at: 1936 5140 1936
Size: 3204 4594
SanDI GG'GwC_CC
Cuts at: 2434 2434
Size: 7798
SapI GCTCTTCn'nnn_
Cuts at: 2180 3071 2180
Size: 891 6907
SbfI CC_TGCA'GG
Cuts at: 5150 5150
Size: 7798
ScaI AGT'ACT
Cuts at: 6046 7758 6046
Size: 1712 6086
SexAI A'CCwGG_T
Cuts at: 2658 2672 2658
Size: 14 7784
SfcI C'TryA_G
Cuts at: 826 1045 2402 2931 3742 3799 4006 4162 4677
Size: 219 1357 529 811 57 207 156 515
Cuts at: 4677 5146 6281 6962 7153 826
Size: 469 1135 681 191 1471
Fragments arranged by size:
1471 1357 1135 811 681 529 515 469
219 207 191 156 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7798
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7798
SmaI CCC'GGG
Cuts at: 1872 2439 2652 5125 1872
Size: 567 213 2473 4545
Fragments arranged by size:
4545 2473 567 213
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3528 5007 5902 6803 7065 820
Size: 197 926 1585 1479 895 901 262 1553
Fragments arranged by size:
1585 1553 1479 926 901 895 262 197
SnaBI TAC'GTA
Cuts at: 493 5589 493
Size: 5096 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7798
SphI G_CATG'C
Cuts at: 1132 2027 4199 5156 1132
Size: 895 2172 957 3774
Fragments arranged by size:
3774 2172 957 895
SspI AAT'ATT
Cuts at: 5 52 5722 5
Size: 47 5670 2081
Fragments arranged by size:
5670 2081 47
StuI AGG'CCT
Cuts at: 1517 2260 4313 1517
Size: 743 2053 5002
Fragments arranged by size:
5002 2053 743
StyI C'CwwG_G
Cuts at: 513 1065 2274 2876 5237 513
Size: 552 1209 602 2361 3074
Fragments arranged by size:
3074 2361 1209 602 552
SwaI ATTT'AAAT
Cuts at: 3544 3544
Size: 7798
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2335 3878 5825 5842 5995 6180 6625
Size: 1255 755 1543 1947 17 153 185 445
Cuts at: 6625 325
Size: 1498
Fragments arranged by size:
1947 1543 1498 1255 755 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2778 6044 7756
Size: 274 80 33 51 1443 801 3266 1712
Cuts at: 7756 96
Size: 138
Fragments arranged by size:
3266 1712 1443 801 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2568 2600 7758 7790 2568
Size: 32 5158 32 2576
Fragments arranged by size:
5158 2576 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7798
XbaI T'CTAG_A
Cuts at: 5134 5134
Size: 7798
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2463 2463
Size: 7798
XhoI C'TCGA_G
Cuts at: 1943 5007 1943
Size: 3064 4734
XmaI C'CCGG_G
Cuts at: 1870 2437 2650 5123 1870
Size: 567 213 2473 4545
Fragments arranged by size:
4545 2473 567 213
XmnI GAAnn'nnTTC
Cuts at: 3066 3274 3566 3650 3818 4397 4715 5927 3066
Size: 208 292 84 168 579 318 1212 4937
Fragments arranged by size:
4937 1212 579 318 292 208 168 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5606 276
Size: 53 83 186 936 4072 2468
Fragments arranged by size:
4072 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI PvuII RsrII SacII SfiI
SfoI SrfI