Restriction map for
pFN21AB9280
(Circular) (Six-base) MAPSORT of: pFN21AB9280.seq Check: 1316 from: 1 to: 6442
pFN21AB9280 6442 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 1937
Size: 1848 4594
Acc65I G'GTAC_C
Cuts at: 3006 3097 3763 3006
Size: 91 666 5685
Fragments arranged by size:
5685 666 91
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4505 5889 6157 1632
Size: 2873 1384 268 1917
Fragments arranged by size:
2873 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2794 820
Size: 197 1777 4468
Fragments arranged by size:
4468 1777 197
AflIII A'CryG_T
Cuts at: 1203 2897 3217 5341 6318 1203
Size: 1694 320 2124 977 1327
Fragments arranged by size:
2124 1694 1327 977 320
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 2491 5171 2491
Size: 2680 3762
AleI CACnn'nnGTG
Cuts at: 1166 3440 1166
Size: 2274 4168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6442
AlwNI CAG_nnn'CTG
Cuts at: 2104 2162 2939 4185 5757 2104
Size: 58 777 1246 1572 2789
Fragments arranged by size:
2789 1572 1246 777 58
ApaLI G'TGCA_C
Cuts at: 3221 4499 5655 3221
Size: 1278 1156 4008
Fragments arranged by size:
4008 1278 1156
ApoI r'AATT_y
Cuts at: 1493 3365 3751 3977 4129 6238 1493
Size: 1872 386 226 152 2109 1697
Fragments arranged by size:
2109 1872 1697 386 226 152
AscI GG'CGCG_CC
Cuts at: 2377 2377
Size: 6442
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 2216 3767 1870
Size: 73 252 21 1551 4545
Fragments arranged by size:
4545 1551 252 73 21
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2321 2477 3006 3097 3763
Size: 325 217 627 534 156 529 91 666
Cuts at: 3763 5218 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 666 627 534 529 325 217
156 91
BanII G_rGCy'C
Cuts at: 1391 3585 3761 1391
Size: 2194 176 4072
Fragments arranged by size:
4072 2194 176
BbeI G_GCGC'C
Cuts at: 2481 2481
Size: 6442
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2918 3513 928
Size: 971 1019 595 3857
Fragments arranged by size:
3857 1019 971 595
BbvCI CC'TCA_GC
Cuts at: 2188 2188
Size: 6442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2527 2561 4633 4667 1903
Size: 34 590 34 2072 34 3678
Fragments arranged by size:
3678 2072 590 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3232 4334 5544 3232
Size: 1102 1210 4130
Fragments arranged by size:
4130 1210 1102
BclI T'GATC_A
Cuts at: 1548 2556 3013 1548
Size: 1008 457 4977
Fragments arranged by size:
4977 1008 457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5053 136
Size: 107 122 71 631 605 3381 1525
Fragments arranged by size:
3381 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 2045 2405 3669 3859 2045
Size: 360 1264 190 4628
Fragments arranged by size:
4628 1264 360 190
Bme1580I G_kGCm'C
Cuts at: 1790 2307 3225 4503 5659 1790
Size: 517 918 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 918 517
BmgBI CAC'GTC
Cuts at: 1318 3655 1318
Size: 2337 4105
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4173 5131 442
Size: 416 53 83 399 24 2756 958 1753
Fragments arranged by size:
2756 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 2638 5102 1239
Size: 1056 343 2464 2579
Fragments arranged by size:
2579 2464 1056 343
Bpu10I CC'TnA_GC
Cuts at: 2188 2506 2188
Size: 318 6124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 4567 5432 5730 2074
Size: 2493 865 298 2786
Fragments arranged by size:
2786 2493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 5105 882
Size: 617 954 2652 2219
Fragments arranged by size:
2652 2219 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 3220 4233 493
Size: 711 2016 1013 2702
Fragments arranged by size:
2702 2016 1013 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2478 2492 4249
Size: 53 83 186 936 498 447 14 1757
Cuts at: 4249 4631 5266 275
Size: 382 635 1451
Fragments arranged by size:
1757 1451 936 635 498 447 382 186
83 53 14
BsaWI w'CCGG_w
Cuts at: 4875 5273 5547 5694 6105 4875
Size: 398 274 147 411 5212
Fragments arranged by size:
5212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2213 2243 1178
Size: 30 165 30 810 30 5377
Fragments arranged by size:
5377 810 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 2292 2845 1186
Size: 956 150 553 4783
Fragments arranged by size:
4783 956 553 150
BseYI C'CCAG_C
Cuts at: 2365 5645 6141 2365
Size: 3280 496 2666
Fragments arranged by size:
3280 2666 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5869
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2042 3892 4653 5681 1540
Size: 275 186 41 1850 761 1028 2301
Fragments arranged by size:
2301 1850 1028 761 275 186 41
BsiHKAI G_wGCw'C
Cuts at: 2223 2875 3225 4503 4588 5659 2223
Size: 652 350 1278 85 1071 3006
Fragments arranged by size:
3006 1278 1071 652 350 85
BsmI GAATG_Cn'
Cuts at: 3953 4046 3953
Size: 93 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2307 2875 3225 3585 3761 4503
Size: 399 433 84 568 350 360 176 742
Cuts at: 4503 4588 5659 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 568 433 399 360 350
176 85 84
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 3231 4331 6061 3231
Size: 1100 1730 3612
Fragments arranged by size:
3612 1730 1100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2007 3797 844
Size: 1163 1790 3489
Fragments arranged by size:
3489 1790 1163
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 2301 3673 4329 1394
Size: 429 221 257 1372 656 3507
Fragments arranged by size:
3507 1372 656 429 257 221
BsrDI GCAATG_nn'
Cuts at: 65 1248 4937 5111 65
Size: 1183 3689 174 1396
Fragments arranged by size:
3689 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2255 2373 5086 5273 1700
Size: 110 445 118 2713 187 2869
Fragments arranged by size:
2869 2713 445 187 118 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 2377 1925
Size: 452 5990
BssSI C'ACGA_G
Cuts at: 2078 4502 5514 2078
Size: 2424 1012 3006
Fragments arranged by size:
3006 2424 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 2162 794
Size: 1310 58 5074
Fragments arranged by size:
5074 1310 58
BstBI TT'CG_AA
Cuts at: 2388 5246 2388
Size: 2858 3584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2239
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 3772 4139 4524 4541 5993 6079 6091 6437
Size: 1800 367 385 17 1452 86 12 346
Cuts at: 6437 1972
Size: 1977
Fragments arranged by size:
1977 1800 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5890
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3062 487
Size: 683 56 1836 3867
Fragments arranged by size:
3867 1836 683 56
BtsI GCAGTG_nn'
Cuts at: 2323 3958 4752 4772 2323
Size: 1635 794 20 3993
Fragments arranged by size:
3993 1635 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2467 3653 5449 809
Size: 507 1151 1186 1796 1802
Fragments arranged by size:
1802 1796 1186 1151 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2039 3889 4778
Size: 54 1425 50 275 18 209 1850 889
Cuts at: 4778 6407 8
Size: 1629 43
Fragments arranged by size:
1850 1629 1425 889 275 209 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2039 3889 1537
Size: 275 227 1850 4090
Fragments arranged by size:
4090 1850 275 227
EarI CTCTTCn'nnn_
Cuts at: 1353 3512 3712 4372 1353
Size: 2159 200 660 3423
Fragments arranged by size:
3423 2159 660 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2277 5029 5403 5549 387
Size: 1064 138 688 2752 374 146 1280
Fragments arranged by size:
2752 1280 1064 688 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2295 2638 4505 5102 5889 6157 1239
Size: 393 663 343 1867 597 787 268 1524
Fragments arranged by size:
1867 1524 787 663 597 393 343 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3688 3688
Size: 6442
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
EcoRV GAT'ATC
Cuts at: 3060 3060
Size: 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 6442
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2246 2481 5589 1678
Size: 568 235 3108 2531
Fragments arranged by size:
3108 2531 568 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 2840 2872 3226
Size: 32 135 32 905 32 331 32 354
Cuts at: 3226 3258 5083 5115 5157 5189 1373
Size: 32 1825 32 42 32 2626
Fragments arranged by size:
2626 1825 905 354 331 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3786 4032 669
Size: 988 281 1848 246 3079
Fragments arranged by size:
3079 1848 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KasI G'GCGC_C
Cuts at: 2477 2477
Size: 6442
KpnI G_GTAC'C
Cuts at: 3010 3101 3767 3010
Size: 91 666 5685
Fragments arranged by size:
5685 666 91
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 2256 5556 5740 1305
Size: 799 152 3300 184 2007
Fragments arranged by size:
3300 2007 799 184 152
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4620
Fragments arranged by size:
4620 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2710 3440 4400 4759 4918 518
Size: 648 1544 730 960 359 159 2042
Fragments arranged by size:
2042 1544 960 730 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2162 2177 2759 3247 3272 3858 4535
Size: 407 149 15 582 488 25 586 677
Cuts at: 4535 5683 5928 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 586 582 488 407 245
149 25 15
NarI GG'CG_CC
Cuts at: 2478 2478
Size: 6442
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5890
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 2039 3889 2039
Size: 1850 4592
NspI r_CATG'y
Cuts at: 1132 1229 2434 3800 5345 1132
Size: 97 1205 1366 1545 2229
Fragments arranged by size:
2229 1545 1366 1205 97
PasI CC'CwG_GG
Cuts at: 1348 3578 1348
Size: 2230 4212
PciI A'CATG_T
Cuts at: 5341 5341
Size: 6442
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2083 1108
Size: 975 5467
PfoI T'CCnGG_A
Cuts at: 3689 3689
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PmlI CAC'GTG
Cuts at: 3220 3220
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2342 2374 2342
Size: 32 6410
PstI C_TGCA'G
Cuts at: 830 2018 2109 2158 3794 830
Size: 1188 91 49 1636 3478
Fragments arranged by size:
3478 1636 1188 91 49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
PvuII CAG'CTG
Cuts at: 2013 3247 3272 2013
Size: 1234 25 5183
Fragments arranged by size:
5183 1234 25
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 2014 2105 2154 3177 3790 4925 5606
Size: 219 969 91 49 1023 613 1135 681
Cuts at: 5606 5797 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1023 969 681 613 219 191
91 49
SfoI GGC'GCC
Cuts at: 2479 2479
Size: 6442
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 2373 1700
Size: 673 5769
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 2794 4546 5447 5709 820
Size: 197 926 110 741 1752 901 262 1553
Fragments arranged by size:
1752 1553 926 901 741 262 197 110
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 3800 1132
Size: 2668 3774
SspI AAT'ATT
Cuts at: 5 52 4366 5
Size: 47 4314 2081
Fragments arranged by size:
4314 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6442
StyI C'CwwG_G
Cuts at: 513 1065 3040 3881 513
Size: 552 1975 841 3074
Fragments arranged by size:
3074 1975 841 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 2540 3137 4469 4486 4639 4824
Size: 1255 897 63 597 1332 17 153 185
Cuts at: 4824 5269 325
Size: 445 1498
Fragments arranged by size:
1498 1332 1255 897 597 445 185 153
63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2941 3280 4688
Size: 274 80 33 51 1443 964 339 1408
Cuts at: 4688 6400 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1408 964 339 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 6402 6434 2213
Size: 32 4157 32 2221
Fragments arranged by size:
4157 2221 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 2858 3778 2858
Size: 920 5522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2761 3575 2761
Size: 814 5628
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 4571 4571
Size: 6442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI
NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI