Restriction map for
pFN21AB9273
(Circular) (Six-base) MAPSORT of: pFN21AB9273.seq Check: 8763 from: 1 to: 5833
pFN21AB9273 5833 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2789 3176 1937
Size: 852 387 4594
Fragments arranged by size:
4594 852 387
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 2690 3896 5280 5548 1632
Size: 995 63 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 995 268 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 2868 4732 5709 1203
Size: 1665 1864 977 1327
Fragments arranged by size:
1864 1665 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2958 3040 1184
Size: 1774 82 3977
Fragments arranged by size:
3977 1774 82
AlwNI CAG_nnn'CTG
Cuts at: 2713 3576 5148 2713
Size: 863 1572 3398
Fragments arranged by size:
3398 1572 863
ApaLI G'TGCA_C
Cuts at: 2933 3890 5046 2933
Size: 957 1156 3720
Fragments arranged by size:
3720 1156 957
ApoI r'AATT_y
Cuts at: 1493 2321 2686 3142 3368 3520 5629 1493
Size: 828 365 456 226 152 2109 1697
Fragments arranged by size:
2109 1697 828 456 365 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3154 4609 618
Size: 325 217 627 1367 1455 1842
Fragments arranged by size:
1842 1455 1367 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2832 3152 1391
Size: 1441 320 4072
Fragments arranged by size:
4072 1441 320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2754 928
Size: 971 855 4007
Fragments arranged by size:
4007 971 855
BbvCI CC'TCA_GC
Cuts at: 2075 2918 2075
Size: 843 4990
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4024 4058 1903
Size: 34 50 34 2003 34 3678
Fragments arranged by size:
3678 2003 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2449 4444
Size: 107 122 71 631 605 337 440 1995
Cuts at: 4444 136
Size: 1525
Fragments arranged by size:
1995 1525 631 605 440 337 122 107
71
BglII A'GATC_T
Cuts at: 2190 5828 2190
Size: 3638 2195
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2937 3894 5050 1790
Size: 1147 957 1156 2573
Fragments arranged by size:
2573 1156 1147 957
BmgBI CAC'GTC
Cuts at: 1318 2023 2869 1318
Size: 705 846 4282
Fragments arranged by size:
4282 846 705
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 3564 4522
Size: 416 53 83 399 24 711 1436 958
Cuts at: 4522 442
Size: 1753
Fragments arranged by size:
1753 1436 958 711 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 3084 4493 1239
Size: 1241 604 1409 2579
Fragments arranged by size:
2579 1409 1241 604
Bpu10I CC'TnA_GC
Cuts at: 2075 2918 2075
Size: 843 4990
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4496 882
Size: 617 2997 2219
Fragments arranged by size:
2997 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 2839 3624 493
Size: 711 1020 615 785 2702
Fragments arranged by size:
2702 1020 785 711 615
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 2575 2963 1186
Size: 1297 92 388 4056
Fragments arranged by size:
4056 1297 388 92
BseYI C'CCAG_C
Cuts at: 2896 3077 3120 5036 5532 2896
Size: 181 43 1916 496 3197
Fragments arranged by size:
3197 1916 496 181 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2937 3894 3979 5050 2937
Size: 957 85 1071 3720
Fragments arranged by size:
3720 1071 957 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2832 2937 3152 3894 3979 5050 1391
Size: 399 1042 105 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 1042 742 399 215 105 85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2922 3188 844
Size: 2078 266 3489
Fragments arranged by size:
3489 2078 266
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2370 4328 4502 65
Size: 1183 1122 1958 174 1396
Fragments arranged by size:
1958 1396 1183 1122 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 2904 96
Size: 2808 3025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2014 794
Size: 1220 4613
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 2087 1184
Size: 903 4930
BstYI r'GATC_y
Cuts at: 1972 2190 3067 3163 3530 3915 3932 5384 5470
Size: 218 877 96 367 385 17 1452 86
Cuts at: 5470 5482 5828 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 877 385 367 346 218 96
86 17 12
BstZ17I GTA'TAC
Cuts at: 2790 2790
Size: 5833
Bsu36I CC'TnA_GG
Cuts at: 2558 2558
Size: 5833
BtgI C'CryG_G
Cuts at: 513 1065 2058 513
Size: 552 993 4288
Fragments arranged by size:
4288 993 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 2515 3137 2515
Size: 622 5211
DraIII CAC_nnn'GTG
Cuts at: 2026 2026
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 3127 3280
Size: 54 1425 50 275 18 231 1066 153
Cuts at: 3280 4169 5798 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1066 889 275 231 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2675 3763 1353
Size: 1322 1088 3423
Fragments arranged by size:
3423 1322 1088
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 2993 4420 4794 4940 387
Size: 1064 138 506 898 1427 374 146 1280
Fragments arranged by size:
1427 1280 1064 898 506 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2480 2627 2690 3084 3896 4493 5280
Size: 393 848 147 63 394 812 597 787
Cuts at: 5280 5548 1239
Size: 268 1524
Fragments arranged by size:
1524 848 812 787 597 394 393 268
147 63
EcoO109I rG'GnC_Cy
Cuts at: 2911 2911
Size: 5833
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2058 4980 1678
Size: 380 2922 2531
Fragments arranged by size:
2922 2531 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2275 2307 4474 4506 4548
Size: 32 135 32 703 32 2167 32 42
Cuts at: 4548 4580 1373
Size: 32 2626
Fragments arranged by size:
2626 2167 703 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4947 5131 1305
Size: 3642 184 2007
Fragments arranged by size:
3642 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3129 10
Size: 54 1425 343 1297 2714
Fragments arranged by size:
2714 1425 1297 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 3791 4150 4309 518
Size: 648 1497 1128 359 159 2042
Fragments arranged by size:
2042 1497 1128 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2504 3061 3249 3926 5074 5319 1606
Size: 898 557 188 677 1148 245 2120
Fragments arranged by size:
2120 1148 898 677 557 245 188
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5281
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2668 3191 4736 1132
Size: 97 1439 523 1545 2229
Fragments arranged by size:
2229 1545 1439 523 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3013 1108
Size: 1905 3928
PfoI T'CCnGG_A
Cuts at: 2970 2970
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2140 3902 3934 5415 5447 2108
Size: 32 1762 32 1481 32 2494
Fragments arranged by size:
2494 1762 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2911 2911
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 2718 3185 830
Size: 1888 467 3478
Fragments arranged by size:
3478 1888 467
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2504 2504
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SexAI A'CCwGG_T
Cuts at: 2091 2725 2091
Size: 634 5199
SfcI C'TryA_G
Cuts at: 826 1045 2714 3181 4316 4997 5188 826
Size: 219 1669 467 1135 681 191 1471
Fragments arranged by size:
1669 1471 1135 681 467 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2224 3624 493
Size: 1731 1400 2702
Fragments arranged by size:
2702 1731 1400
SpeI A'CTAG_T
Cuts at: 152 3019 152
Size: 2867 2966
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 2012 1517
Size: 495 5338
StyI C'CwwG_G
Cuts at: 513 1065 2793 3272 513
Size: 552 1728 479 3074
Fragments arranged by size:
3074 1728 552 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2910 3860 3877 4030 4215 4660 325
Size: 1255 1330 950 17 153 185 445 1498
Fragments arranged by size:
1498 1330 1255 950 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2147 2904 4079
Size: 274 80 33 51 1443 170 757 1175
Cuts at: 4079 5791 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1175 757 274 170 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2723 2755 5793 5825 2723
Size: 32 3038 32 2731
Fragments arranged by size:
3038 2731 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2727 2855 1316
Size: 1411 128 4294
Fragments arranged by size:
4294 1411 128
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 3117 2575
Size: 542 5291
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI