Restriction map for
pFN21AB9268
(Circular) (Six-base) MAPSORT of: pFN21AB9268.seq Check: 8219 from: 1 to: 6433
pFN21AB9268 6433 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4243 278
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3776 1937
Size: 1839 4594
Acc65I G'GTAC_C
Cuts at: 3754 3754
Size: 6433
AclI AA'CG_TT
Cuts at: 1563 4560 4933 1563
Size: 2997 373 3063
Fragments arranged by size:
3063 2997 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2406 2774 4496 5880 6148 1632
Size: 605 169 368 1722 1384 268 1917
Fragments arranged by size:
1917 1722 1384 605 368 268 169
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6236
AflIII A'CryG_T
Cuts at: 1203 2086 5332 6309 1203
Size: 883 3246 977 1327
Fragments arranged by size:
3246 1327 977 883
AgeI A'CCGG_T
Cuts at: 5264 5264
Size: 6433
AhdI GACnn_n'nnGTC
Cuts at: 3116 5162 3116
Size: 2046 4387
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6433
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6433
AlwNI CAG_nnn'CTG
Cuts at: 2932 4176 5748 2932
Size: 1244 1572 3617
Fragments arranged by size:
3617 1572 1244
ApaLI G'TGCA_C
Cuts at: 4490 5646 4490
Size: 1156 5277
ApoI r'AATT_y
Cuts at: 1493 3437 3742 3968 4120 6229 1493
Size: 1944 305 226 152 2109 1697
Fragments arranged by size:
2109 1944 1697 305 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4987 160
Size: 872 3955 1606
Fragments arranged by size:
3955 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
AvaI C'yCGr_G
Cuts at: 1870 1943 3758 1870
Size: 73 1815 4545
Fragments arranged by size:
4545 1815 73
BamHI G'GATC_C
Cuts at: 3763 4130 3763
Size: 367 6066
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 3709 3754 5209 618
Size: 325 217 627 413 1509 45 1455 1842
Fragments arranged by size:
1842 1509 1455 627 413 325 217 45
BanII G_rGCy'C
Cuts at: 1391 3669 3752 1391
Size: 2278 83 4072
Fragments arranged by size:
4072 2278 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2109 2412 928
Size: 971 210 303 4949
Fragments arranged by size:
4949 971 303 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2825 2859 4624 4658 1903
Size: 34 50 34 804 34 1765 34 3678
Fragments arranged by size:
3678 1765 804 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2568 4325 5535 2568
Size: 1757 1210 3466
Fragments arranged by size:
3466 1757 1210
BclI T'GATC_A
Cuts at: 1548 3034 3171 1548
Size: 1486 137 4810
Fragments arranged by size:
4810 1486 137
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5044 136
Size: 107 122 71 631 605 3372 1525
Fragments arranged by size:
3372 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6428 6428
Size: 6433
BlpI GC'TnA_GC
Cuts at: 3850 3850
Size: 6433
Bme1580I G_kGCm'C
Cuts at: 1790 2205 3207 4494 5650 1790
Size: 415 1002 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 1002 415
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6433
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2360 2459 4164
Size: 416 53 83 399 24 943 99 1705
Cuts at: 4164 5122 442
Size: 958 1753
Fragments arranged by size:
1753 1705 958 943 416 399 99 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6433
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 6433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5093 1239
Size: 3854 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4558 5423 5721 4558
Size: 865 298 5270
Fragments arranged by size:
5270 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3692 5096 882
Size: 617 2193 1404 2219
Fragments arranged by size:
2219 2193 1404 617
BsaAI yAC'GTr
Cuts at: 493 1204 4224 493
Size: 711 3020 2702
Fragments arranged by size:
3020 2702 711
BsaBI GATnn'nnATC
Cuts at: 2699 6427 2699
Size: 3728 2705
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4240 4622 5257 275
Size: 53 83 186 936 2707 382 635 1451
Fragments arranged by size:
2707 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2197 4866 5264 5538 5685 6096 2197
Size: 2669 398 274 147 411 2534
Fragments arranged by size:
2669 2534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6208
Fragments arranged by size:
6208 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2526 2529 2684 1186
Size: 1340 3 155 4935
Fragments arranged by size:
4935 1340 155 3
BseYI C'CCAG_C
Cuts at: 2073 2759 3183 5636 6132 2073
Size: 686 424 2453 496 2374
Fragments arranged by size:
2453 2374 686 496 424
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3570 1163
Size: 573 1834 4026
Fragments arranged by size:
4026 1834 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3883 4644 5672 1540
Size: 275 186 1882 761 1028 2301
Fragments arranged by size:
2301 1882 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3669 4494 4579 5650 3669
Size: 825 85 1071 4452
Fragments arranged by size:
4452 1071 825 85
BsmI GAATG_Cn'
Cuts at: 2146 3026 3656 3944 4037 2146
Size: 880 630 288 93 4542
Fragments arranged by size:
4542 880 630 288 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2899 2899
Size: 6433
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 3207 3669 3752 4494 4579 5650
Size: 399 415 1002 462 83 742 85 1071
Cuts at: 5650 1391
Size: 2174
Fragments arranged by size:
2174 1071 1002 742 462 415 399 85
83
BspEI T'CCGG_A
Cuts at: 6096 6096
Size: 6433
BspHI T'CATG_A
Cuts at: 2164 2505 4322 6052 2164
Size: 341 1817 1730 2545
Fragments arranged by size:
2545 1817 1730 341
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3788 844
Size: 2944 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4320 1394
Size: 429 2497 3507
Fragments arranged by size:
3507 2497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4928 5102 65
Size: 1183 3680 174 1396
Fragments arranged by size:
3680 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5077 5264 1700
Size: 110 3267 187 2869
Fragments arranged by size:
3267 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2332 3289 96
Size: 2236 957 3240
Fragments arranged by size:
3240 2236 957
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6433
BssSI C'ACGA_G
Cuts at: 4493 5505 4493
Size: 1012 5421
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6433
BstBI TT'CG_AA
Cuts at: 5237 5237
Size: 6433
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6433
BstXI CCAn_nnnn'nTGG
Cuts at: 3191 3720 3191
Size: 529 5904
BstYI r'GATC_y
Cuts at: 1972 2024 3763 4130 4515 4532 5984 6070 6082
Size: 52 1739 367 385 17 1452 86 12
Cuts at: 6082 6428 1972
Size: 346 1977
Fragments arranged by size:
1977 1739 1452 385 367 346 86 52
17 12
Bsu36I CC'TnA_GG
Cuts at: 2598 2598
Size: 6433
BtgI C'CryG_G
Cuts at: 513 1065 2212 3132 513
Size: 552 1147 920 3814
Fragments arranged by size:
3814 1147 920 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5694
Fragments arranged by size:
5694 683 56
BtsI GCAGTG_nn'
Cuts at: 3949 4743 4763 3949
Size: 794 20 5619
Fragments arranged by size:
5619 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6398
DraI TTT'AAA
Cuts at: 3737 3737
Size: 6433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5440 809
Size: 507 4124 1802
Fragments arranged by size:
4124 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2241 2496
Size: 54 1425 50 275 18 399 12 255
Cuts at: 2496 3880 4769 6398 8
Size: 1384 889 1629 43
Fragments arranged by size:
1629 1425 1384 889 399 275 255 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3880 1537
Size: 275 2068 4090
Fragments arranged by size:
4090 2068 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 3023 4363 1353
Size: 888 782 1340 3423
Fragments arranged by size:
3423 1340 888 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5020 5394 5540 387
Size: 1064 138 3431 374 146 1280
Fragments arranged by size:
3431 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3667 3667
Size: 6433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2406 2774 4496 5093 5880 6148
Size: 393 605 169 368 1722 597 787 268
Cuts at: 6148 1239
Size: 1524
Fragments arranged by size:
1722 1524 787 605 597 393 368 268
169
EcoRI G'AATT_C
Cuts at: 3742 4120 3742
Size: 378 6055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2780 2780
Size: 6433
FspI TGC'GCA
Cuts at: 4939 4939
Size: 6433
HaeII r_GCGC'y
Cuts at: 1678 5580 1678
Size: 3902 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2605 2637 2663 2695 3067
Size: 32 135 32 1033 32 26 32 372
Cuts at: 3067 3099 3102 3134 5074 5106 5148 5180 1373
Size: 32 3 32 1940 32 42 32 2626
Fragments arranged by size:
2626 1940 1033 372 135 42 32 32
32 32 32 32 32 32 26 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2019 2569 3777 4023 669
Size: 988 281 81 550 1208 246 3079
Fragments arranged by size:
3079 1208 988 550 281 246 81
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6433
HpaI GTT'AAC
Cuts at: 4023 4023
Size: 6433
KpnI G_GTAC'C
Cuts at: 3758 3758
Size: 6433
MfeI C'AATT_G
Cuts at: 4032 4032
Size: 6433
MluI A'CGCG_T
Cuts at: 6309 6309
Size: 6433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2967 3375 5547 5731 1305
Size: 1662 408 2172 184 2007
Fragments arranged by size:
2172 2007 1662 408 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 10
Size: 54 1425 343 666 3945
Fragments arranged by size:
3945 1425 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2181 2547 4391 4750 4909 518
Size: 648 1015 366 1844 359 159 2042
Fragments arranged by size:
2042 1844 1015 648 366 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 3050 3849 4526 5674 5919 1606
Size: 471 973 799 677 1148 245 2120
Fragments arranged by size:
2120 1148 973 799 677 471 245
NcoI C'CATG_G
Cuts at: 513 1065 2212 513
Size: 552 1147 4734
Fragments arranged by size:
4734 1147 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6433
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6433
NotI GC'GGCC_GC
Cuts at: 3880 3880
Size: 6433
NspI r_CATG'y
Cuts at: 1132 1229 2090 3791 5336 1132
Size: 97 861 1701 1545 2229
Fragments arranged by size:
2229 1701 1545 861 97
PasI CC'CwG_GG
Cuts at: 1348 2853 1348
Size: 1505 4928
PciI A'CATG_T
Cuts at: 2086 5332 2086
Size: 3246 3187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6433
PfoI T'CCnGG_A
Cuts at: 2883 2883
Size: 6433
PmeI GTTT'AAAC
Cuts at: 3737 3737
Size: 6433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4534 6015 6047 4502
Size: 32 1481 32 4888
Fragments arranged by size:
4888 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2040 1613
Size: 427 6006
PsiI TTA'TAA
Cuts at: 3461 3632 4003 3461
Size: 171 371 5891
Fragments arranged by size:
5891 371 171
PstI C_TGCA'G
Cuts at: 830 2082 2524 3785 830
Size: 1252 442 1261 3478
Fragments arranged by size:
3478 1261 1252 442
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6433
PvuII CAG'CTG
Cuts at: 2077 3050 2077
Size: 973 5460
SacI G_AGCT'C
Cuts at: 3669 3669
Size: 6433
SalI G'TCGA_C
Cuts at: 1936 3775 1936
Size: 1839 4594
SapI GCTCTTCn'nnn_
Cuts at: 2241 2241
Size: 6433
SbfI CC_TGCA'GG
Cuts at: 3785 3785
Size: 6433
ScaI AGT'ACT
Cuts at: 4681 6393 4681
Size: 1712 4721
SfcI C'TryA_G
Cuts at: 826 1045 2078 2520 3781 4916 5597 5788 826
Size: 219 1033 442 1261 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 1033 681 442 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6433
SmaI CCC'GGG
Cuts at: 1872 3760 1872
Size: 1888 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4537 5438 5700 820
Size: 197 926 2594 901 262 1553
Fragments arranged by size:
2594 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4224 493
Size: 3731 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6433
SphI G_CATG'C
Cuts at: 1132 3791 1132
Size: 2659 3774
SspI AAT'ATT
Cuts at: 5 52 2919 4357 5
Size: 47 2867 1438 2081
Fragments arranged by size:
2867 2081 1438 47
StuI AGG'CCT
Cuts at: 1517 2596 1517
Size: 1079 5354
StyI C'CwwG_G
Cuts at: 513 1065 2212 2226 3532 3872 513
Size: 552 1147 14 1306 340 3074
Fragments arranged by size:
3074 1306 1147 552 340 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3197 3351 3697 4460 4477 4630 4815
Size: 1255 1617 154 346 763 17 153 185
Cuts at: 4815 5260 325
Size: 445 1498
Fragments arranged by size:
1617 1498 1255 763 445 346 185 154
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2332 3289 4679
Size: 274 80 33 51 1443 355 957 1390
Cuts at: 4679 6391 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1390 957 355 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6393 6425 6393
Size: 32 6401
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6433
XbaI T'CTAG_A
Cuts at: 3769 3769
Size: 6433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3239 3239
Size: 6433
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6433
XmaI C'CCGG_G
Cuts at: 1870 3758 1870
Size: 1888 4545
XmnI GAAnn'nnTTC
Cuts at: 2250 3564 4562 2250
Size: 1314 998 4121
Fragments arranged by size:
4121 1314 998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4241 276
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI