Restriction map for
pFN21AB9259
(Circular) (Six-base) MAPSORT of: pFN21AB9259.seq Check: 8147 from: 1 to: 7642
pFN21AB9259 7642 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3100 3907 3976 3100
Size: 807 69 6766
Fragments arranged by size:
6766 807 69
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5452 278
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4985 1937
Size: 3048 4594
Acc65I G'GTAC_C
Cuts at: 4963 4963
Size: 7642
AclI AA'CG_TT
Cuts at: 1563 5769 6142 1563
Size: 4206 373 3063
Fragments arranged by size:
4206 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2202 3598 5705 7089 7357 1632
Size: 570 1396 2107 1384 268 1917
Fragments arranged by size:
2107 1917 1396 1384 570 268
AfeI AGC'GCT
Cuts at: 4022 4796 4022
Size: 774 6868
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7445
AflIII A'CryG_T
Cuts at: 1203 6541 7518 1203
Size: 5338 977 1327
Fragments arranged by size:
5338 1327 977
AgeI A'CCGG_T
Cuts at: 3143 6473 3143
Size: 3330 4312
AhdI GACnn_n'nnGTC
Cuts at: 2981 6371 2981
Size: 3390 4252
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4390 1184
Size: 3206 4436
AlwNI CAG_nnn'CTG
Cuts at: 3415 4618 5385 6957 3415
Size: 1203 767 1572 4100
Fragments arranged by size:
4100 1572 1203 767
ApaI G_GGCC'C
Cuts at: 2084 2098 2190 2297 2454 2598 3728 4164 4171
Size: 14 92 107 157 144 1130 436 7
Cuts at: 4171 2084
Size: 5555
Fragments arranged by size:
5555 1130 436 157 144 107 92 14
7
ApaLI G'TGCA_C
Cuts at: 5699 6855 5699
Size: 1156 6486
ApoI r'AATT_y
Cuts at: 1493 4951 5177 5329 7438 1493
Size: 3458 226 152 2109 1697
Fragments arranged by size:
3458 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6196 160
Size: 872 5164 1606
Fragments arranged by size:
5164 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 2152 2453 2644 2917 3037 3810 4164
Size: 73 209 301 191 273 120 773 354
Cuts at: 4164 4481 4922 4967 1870
Size: 317 441 45 4545
Fragments arranged by size:
4545 773 441 354 317 301 273 209
191 120 73 45
BamHI G'GATC_C
Cuts at: 3336 4582 4972 5339 3336
Size: 1246 390 367 5639
Fragments arranged by size:
5639 1246 390 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 2517 2569 2760 3112
Size: 325 217 627 555 175 52 191 352
Cuts at: 3112 3796 3802 4440 4963 6418 618
Size: 684 6 638 523 1455 1842
Fragments arranged by size:
1842 1455 684 638 627 555 523 352
325 217 191 175 52 6
BanII G_rGCy'C
Cuts at: 1391 2084 2098 2114 2139 2190 2297 2454 2490
Size: 693 14 16 25 51 107 157 36
Cuts at: 2490 2504 2598 2651 3063 3353 3728 4152 4164
Size: 14 94 53 412 290 375 424 12
Cuts at: 4164 4171 4961 1391
Size: 7 790 4072
Fragments arranged by size:
4072 790 693 424 412 375 290 157
107 94 53 51 36 25 16 14
14 12 7
BbeI G_GCGC'C
Cuts at: 2573 3800 2573
Size: 1227 6415
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2319 4107 4177 928
Size: 971 420 1788 70 4393
Fragments arranged by size:
4393 1788 971 420 70
BbvCI CC'TCA_GC
Cuts at: 2604 2604
Size: 7642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5833 5867 1903
Size: 34 3896 34 3678
Fragments arranged by size:
3896 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3026 5534 6744 3026
Size: 2508 1210 3924
Fragments arranged by size:
3924 2508 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7642
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3527 4077 4091
Size: 107 122 71 631 605 1855 550 14
Cuts at: 4091 6253 136
Size: 2162 1525
Fragments arranged by size:
2162 1855 1525 631 605 550 122 107
71 14
BglII A'GATC_T
Cuts at: 4911 7637 4911
Size: 2726 4916
BlpI GC'TnA_GC
Cuts at: 3392 5059 3392
Size: 1667 5975
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2098 2152 2190 2297 2347 2454 2477
Size: 294 14 54 38 107 50 107 23
Cuts at: 2477 2598 3728 4164 4171 4605 5703 6859 1790
Size: 121 1130 436 7 434 1098 1156 2573
Fragments arranged by size:
2573 1156 1130 1098 436 434 294 121
107 107 54 50 38 23 14 7
BmgBI CAC'GTC
Cuts at: 1318 2077 1318
Size: 759 6883
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3136 5373 6331
Size: 416 53 83 399 24 1719 2237 958
Cuts at: 6331 442
Size: 1753
Fragments arranged by size:
2237 1753 1719 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 3946 3988 2143
Size: 1803 42 5797
Fragments arranged by size:
5797 1803 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 2585 2643 2673 2891 3277 3437 3470
Size: 859 487 58 30 218 386 160 33
Cuts at: 3470 3809 4079 4085 4451 4514 4697 4736 6302
Size: 339 270 6 366 63 183 39 1566
Cuts at: 6302 1239
Size: 2579
Fragments arranged by size:
2579 1566 859 487 386 366 339 270
218 183 160 63 58 39 33 30
6
Bpu10I CC'TnA_GC
Cuts at: 2192 2604 2192
Size: 412 7230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2920 3525 4652 4676 5767 6632 6930 2920
Size: 605 1127 24 1091 865 298 3632
Fragments arranged by size:
3632 1127 1091 865 605 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3068 6305 882
Size: 617 1569 3237 2219
Fragments arranged by size:
3237 2219 1569 617
BsaAI yAC'GTr
Cuts at: 493 1204 4343 5433 493
Size: 711 3139 1090 2702
Fragments arranged by size:
3139 2702 1090 711
BsaBI GATnn'nnATC
Cuts at: 4916 7636 4916
Size: 2720 4922
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2570 3468 3797 5449
Size: 53 83 186 936 1037 898 329 1652
Cuts at: 5449 5831 6466 275
Size: 382 635 1451
Fragments arranged by size:
1652 1451 1037 936 898 635 382 329
186 83 53
BsaWI w'CCGG_w
Cuts at: 3070 3143 6075 6473 6747 6894 7305 3070
Size: 73 2932 398 274 147 411 3407
Fragments arranged by size:
3407 2932 411 398 274 147 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3410 3437 3440 3467 4332
Size: 30 165 30 2007 27 3 27 865
Cuts at: 4332 4362 4424 4454 4515 4545 1178
Size: 30 62 30 61 30 4275
Fragments arranged by size:
4275 2007 865 165 62 61 30 30
30 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2640 2740 3230 3280 3554 3592 3878 4499
Size: 1454 100 490 50 274 38 286 621
Cuts at: 4499 4742 1186
Size: 243 4086
Fragments arranged by size:
4086 1454 621 490 286 274 243 100
50 38
BseYI C'CCAG_C
Cuts at: 2044 2107 4152 6845 7341 2044
Size: 63 2045 2693 496 2345
Fragments arranged by size:
2693 2345 2045 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 2932 3935 4004 4067 1163
Size: 573 819 377 1003 69 63 4738
Fragments arranged by size:
4738 1003 819 573 377 69 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2026 3720 3902 4312 5092 5853
Size: 275 186 25 1694 182 410 780 761
Cuts at: 5853 6881 1540
Size: 1028 2301
Fragments arranged by size:
2301 1694 1028 780 761 410 275 186
182 25
BsiHKAI G_wGCw'C
Cuts at: 3353 4437 5703 5788 6859 3353
Size: 1084 1266 85 1071 4136
Fragments arranged by size:
4136 1266 1084 1071 85
BsiWI C'GTAC_G
Cuts at: 4242 4242
Size: 7642
BsmI GAATG_Cn'
Cuts at: 5153 5246 5153
Size: 93 7549
BsmBI CGTCTCn'nnnn_
Cuts at: 3917 3917
Size: 7642
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2098 2114 2139 2152 2190 2297
Size: 399 294 14 16 25 13 38 107
Cuts at: 2297 2347 2454 2477 2490 2504 2598 2651 3063
Size: 50 107 23 13 14 94 53 412
Cuts at: 3063 3353 3728 4152 4164 4171 4437 4605 4961
Size: 290 375 424 12 7 266 168 356
Cuts at: 4961 5703 5788 6859 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 412 399 375 356
294 290 266 168 107 107 94 85
53 50 38 25 23 16 14 14
13 13 12 7
BspEI T'CCGG_A
Cuts at: 3070 7305 3070
Size: 4235 3407
BspHI T'CATG_A
Cuts at: 5531 7261 5531
Size: 1730 5912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 3100 3486 3907 3976 4997 844
Size: 1268 988 386 421 69 1021 3489
Fragments arranged by size:
3489 1268 1021 988 421 386 69
BsrBI CCG'CTC
Cuts at: 1394 1823 3186 3222 5529 1394
Size: 429 1363 36 2307 3507
Fragments arranged by size:
3507 2307 1363 429 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 6137 6311 65
Size: 1183 4889 174 1396
Fragments arranged by size:
4889 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3143 3530 3799 4252 4307 4783 4815
Size: 110 1333 387 269 453 55 476 32
Cuts at: 4815 6286 6473 1700
Size: 1471 187 2869
Fragments arranged by size:
2869 1471 1333 476 453 387 269 187
110 55 32
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7642
BssHII G'CGCG_C
Cuts at: 1925 3779 4832 4858 1925
Size: 1854 1053 26 4709
Fragments arranged by size:
4709 1854 1053 26
BssSI C'ACGA_G
Cuts at: 3701 5702 6714 3701
Size: 2001 1012 4629
Fragments arranged by size:
4629 2001 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7642
BstBI TT'CG_AA
Cuts at: 6446 6446
Size: 7642
BstEII G'GTnAC_C
Cuts at: 1184 2012 3134 1184
Size: 828 1122 5692
Fragments arranged by size:
5692 1122 828
BstXI CCAn_nnnn'nTGG
Cuts at: 2968 3977 2968
Size: 1009 6633
BstYI r'GATC_y
Cuts at: 1972 3162 3336 4488 4500 4582 4911 4972 5339
Size: 1190 174 1152 12 82 329 61 367
Cuts at: 5339 5724 5741 7193 7279 7291 7637 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1190 1152 385 367 346 329
174 86 82 61 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2223 4722 2223
Size: 2499 5143
BtgI C'CryG_G
Cuts at: 513 1065 2017 3204 3462 3771 4086 4809 513
Size: 552 952 1187 258 309 315 723 3346
Fragments arranged by size:
3346 1187 952 723 552 315 309 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3411 487
Size: 683 56 2185 4718
Fragments arranged by size:
4718 2185 683 56
BtsI GCAGTG_nn'
Cuts at: 2475 2500 4235 4513 4587 5158 5952 5972 2475
Size: 25 1735 278 74 571 794 20 4145
Fragments arranged by size:
4145 1735 794 571 278 74 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7607
DraI TTT'AAA
Cuts at: 4946 4946
Size: 7642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6649 809
Size: 507 5333 1802
Fragments arranged by size:
5333 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2287 3717 3899
Size: 54 1425 50 275 18 457 1430 182
Cuts at: 3899 4083 4092 4254 4309 4851 4884 5089 5978
Size: 184 9 162 55 542 33 205 889
Cuts at: 5978 7607 8
Size: 1629 43
Fragments arranged by size:
1629 1430 1425 889 542 457 275 205
184 182 162 55 54 50 43 33
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3717 3899 4309 5089 1537
Size: 275 1905 182 410 780 4090
Fragments arranged by size:
4090 1905 780 410 275 182
EarI CTCTTCn'nnn_
Cuts at: 1353 2605 3987 5572 1353
Size: 1252 1382 1585 3423
Fragments arranged by size:
3423 1585 1382 1252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3736 4478 4734 4862 6229 6603
Size: 1064 138 2147 742 256 128 1367 374
Cuts at: 6603 6749 387
Size: 146 1280
Fragments arranged by size:
2147 1367 1280 1064 742 374 256 146
138 128
EcoICRI GAG'CTC
Cuts at: 3351 3351
Size: 7642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2202 2585 2643 2673 2891 3277
Size: 393 466 104 383 58 30 218 386
Cuts at: 3277 3437 3470 3598 3809 4079 4085 4451 4514
Size: 160 33 128 211 270 6 366 63
Cuts at: 4514 4697 4736 5705 6302 7089 7357 1239
Size: 183 39 969 597 787 268 1524
Fragments arranged by size:
1524 969 787 597 466 393 386 383
366 270 268 218 211 183 160 128
104 63 58 39 33 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2201 2226 2272 3172 4132 2201
Size: 25 46 900 960 5711
Fragments arranged by size:
5711 960 900 46 25
EcoO109I rG'GnC_Cy
Cuts at: 2081 2094 2095 2186 2187 2219 2293 2294 2450
Size: 13 1 91 1 32 74 1 156
Cuts at: 2450 2657 2838 2886 3622 3652 3724 3725 4069
Size: 207 181 48 736 30 72 1 344
Cuts at: 4069 4108 4161 4168 4615 4633 4738 2081
Size: 39 53 7 447 18 105 4985
Fragments arranged by size:
4985 736 447 344 207 181 156 105
91 74 72 53 48 39 32 30
18 13 7 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4951 5329 4951
Size: 378 7264
FseI GG_CCGG'CC
Cuts at: 4311 4311
Size: 7642
FspI TGC'GCA
Cuts at: 6148 6148
Size: 7642
HaeII r_GCGC'y
Cuts at: 1678 2465 2573 2917 3739 3800 4024 4798 4899
Size: 787 108 344 822 61 224 774 101
Cuts at: 4899 6789 1678
Size: 1890 2531
Fragments arranged by size:
2531 1890 822 787 774 344 224 108
101 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2963 2967 2995
Size: 32 135 32 623 32 736 4 28
Cuts at: 2995 2999 3437 3469 6283 6315 6357 6389 1373
Size: 4 438 32 2814 32 42 32 2626
Fragments arranged by size:
2814 2626 736 623 438 135 42 32
32 32 32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4986 5232 669
Size: 988 281 3048 246 3079
Fragments arranged by size:
3079 3048 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7642
HpaI GTT'AAC
Cuts at: 5232 5232
Size: 7642
KasI G'GCGC_C
Cuts at: 2569 3796 2569
Size: 1227 6415
KpnI G_GTAC'C
Cuts at: 4967 4967
Size: 7642
MfeI C'AATT_G
Cuts at: 5241 5241
Size: 7642
MluI A'CGCG_T
Cuts at: 7518 7518
Size: 7642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2907 6756 6940 1305
Size: 1602 3849 184 2007
Fragments arranged by size:
3849 2007 1602 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2289 4085 4094 10
Size: 54 1425 343 457 1796 9 3558
Fragments arranged by size:
3558 1796 1425 457 343 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2954 2960 3911 4403 5600 5959 6118
Size: 648 1788 6 951 492 1197 359 159
Cuts at: 6118 518
Size: 2042
Fragments arranged by size:
2042 1788 1197 951 648 492 359 159
6
MspA1I CmG'CkG
Cuts at: 1606 2237 2937 3415 3581 3889 4704 4822 5058
Size: 631 700 478 166 308 815 118 236
Cuts at: 5058 5735 6883 7128 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 700 677 631 478 308
245 236 166 118
NaeI GCC'GGC
Cuts at: 3532 4254 4309 3532
Size: 722 55 6865
Fragments arranged by size:
6865 722 55
NarI GG'CG_CC
Cuts at: 2570 3797 2570
Size: 1227 6415
NcoI C'CATG_G
Cuts at: 513 1065 2017 3204 3771 513
Size: 552 952 1187 567 4384
Fragments arranged by size:
4384 1187 952 567 552
NdeI CA'TA_TG
Cuts at: 387 2070 387
Size: 1683 5959
NgoMIV G'CCGG_C
Cuts at: 3530 4252 4307 3530
Size: 722 55 6865
Fragments arranged by size:
6865 722 55
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NspI r_CATG'y
Cuts at: 1132 1229 2959 3499 5000 6545 1132
Size: 97 1730 540 1501 1545 2229
Fragments arranged by size:
2229 1730 1545 1501 540 97
PasI CC'CwG_GG
Cuts at: 1348 2843 4301 1348
Size: 1495 1458 4689
Fragments arranged by size:
4689 1495 1458
PciI A'CATG_T
Cuts at: 6541 6541
Size: 7642
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2563 2781 3415 3463 4450 4618 4704 1108
Size: 1455 218 634 48 987 168 86 4046
Fragments arranged by size:
4046 1455 987 634 218 168 86 48
PfoI T'CCnGG_A
Cuts at: 2367 4322 4491 4611 4674 4713 2367
Size: 1955 169 120 63 39 5296
Fragments arranged by size:
5296 1955 169 120 63 39
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2886 4615 4633 4738 2886
Size: 1729 18 105 5790
Fragments arranged by size:
5790 1729 105 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7642
PsiI TTA'TAA
Cuts at: 5212 5212
Size: 7642
PspOMI G'GGCC_C
Cuts at: 2080 2094 2186 2293 2450 2594 3724 4160 4167
Size: 14 92 107 157 144 1130 436 7
Cuts at: 4167 2080
Size: 5555
Fragments arranged by size:
5555 1130 436 157 144 107 92 14
7
PstI C_TGCA'G
Cuts at: 830 3111 3743 4116 4994 830
Size: 2281 632 373 878 3478
Fragments arranged by size:
3478 2281 878 632 373
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
PvuII CAG'CTG
Cuts at: 3415 4704 3415
Size: 1289 6353
SacI G_AGCT'C
Cuts at: 3353 3353
Size: 7642
SalI G'TCGA_C
Cuts at: 1936 4984 1936
Size: 3048 4594
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 5890 7602 5890
Size: 1712 5930
SexAI A'CCwGG_T
Cuts at: 2520 2520
Size: 7642
SfcI C'TryA_G
Cuts at: 826 1045 3107 3364 3739 4112 4640 4682 4990
Size: 219 2062 257 375 373 528 42 308
Cuts at: 4990 6125 6806 6997 826
Size: 1135 681 191 1471
Fragments arranged by size:
2062 1471 1135 681 528 375 373 308
257 219 191 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 3527 4077 4091 3527
Size: 550 14 7078
Fragments arranged by size:
7078 550 14
SfoI GGC'GCC
Cuts at: 2571 3798 2571
Size: 1227 6415
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 3143 3799 1700
Size: 1443 656 5543
Fragments arranged by size:
5543 1443 656
SmaI CCC'GGG
Cuts at: 1872 2455 2646 3039 3812 4166 4969 1872
Size: 583 191 393 773 354 803 4545
Fragments arranged by size:
4545 803 773 583 393 354 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2899 3540 4667 4691 5746 6647
Size: 197 926 956 641 1127 24 1055 901
Cuts at: 6647 6909 820
Size: 262 1553
Fragments arranged by size:
1553 1127 1055 956 926 901 641 262
197 24
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7642
SphI G_CATG'C
Cuts at: 1132 3499 5000 1132
Size: 2367 1501 3774
Fragments arranged by size:
3774 2367 1501
SrfI GCCC'GGGC
Cuts at: 2455 2646 2455
Size: 191 7451
SspI AAT'ATT
Cuts at: 5 52 5566 5
Size: 47 5514 2081
Fragments arranged by size:
5514 2081 47
StuI AGG'CCT
Cuts at: 1517 2123 4080 1517
Size: 606 1957 5079
Fragments arranged by size:
5079 1957 606
StyI C'CwwG_G
Cuts at: 513 1065 2017 2598 3078 3204 3771 5081 513
Size: 552 952 581 480 126 567 1310 3074
Fragments arranged by size:
3074 1310 952 581 567 552 480 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2040 2343 2971 3764 5669 5686 5839
Size: 1255 460 303 628 793 1905 17 153
Cuts at: 5839 6024 6469 325
Size: 185 445 1498
Fragments arranged by size:
1905 1498 1255 793 628 460 445 303
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5888 7600 96
Size: 274 80 33 51 1443 3911 1712 138
Fragments arranged by size:
3911 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2693 2725 3321 3353 3410 3442 4332 4364 4424
Size: 32 596 32 57 32 890 32 60
Cuts at: 4424 4456 4515 4547 7602 7634 2693
Size: 32 59 32 3055 32 2701
Fragments arranged by size:
3055 2701 890 596 60 59 57 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7642
XbaI T'CTAG_A
Cuts at: 4978 4978
Size: 7642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3026 3026
Size: 7642
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7642
XmaI C'CCGG_G
Cuts at: 1870 2453 2644 3037 3810 4164 4967 1870
Size: 583 191 393 773 354 803 4545
Fragments arranged by size:
4545 803 773 583 393 354 191
XmnI GAAnn'nnTTC
Cuts at: 5771 5771
Size: 7642
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5450 276
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BstZ17I DraIII EcoRV
FalI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SwaI