Restriction map for
pFN21AB9213
(Circular) (Six-base) MAPSORT of: pFN21AB9213.seq Check: 758 from: 1 to: 7222
pFN21AB9213 7222 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5032 278
Size: 53 83 186 936 3496 2468
Fragments arranged by size:
3496 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4565 1937
Size: 2628 4594
Acc65I G'GTAC_C
Cuts at: 4543 4543
Size: 7222
AclI AA'CG_TT
Cuts at: 1563 4127 5349 5722 1563
Size: 2564 1222 373 3063
Fragments arranged by size:
3063 2564 1222 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2009 2088 2757 3878 3887 4406 5285 6669
Size: 377 79 669 1121 9 519 879 1384
Cuts at: 6669 6937 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1121 879 669 519 377 268
79 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7025
AflIII A'CryG_T
Cuts at: 1203 6121 7098 1203
Size: 4918 977 1327
Fragments arranged by size:
4918 1327 977
AgeI A'CCGG_T
Cuts at: 6053 6053
Size: 7222
AhdI GACnn_n'nnGTC
Cuts at: 5951 5951
Size: 7222
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7222
AlwNI CAG_nnn'CTG
Cuts at: 2084 2582 2614 4965 6537 2084
Size: 498 32 2351 1572 2769
Fragments arranged by size:
2769 2351 1572 498 32
ApaI G_GGCC'C
Cuts at: 3146 3146
Size: 7222
ApaLI G'TGCA_C
Cuts at: 3570 5279 6435 3570
Size: 1709 1156 4357
Fragments arranged by size:
4357 1709 1156
ApoI r'AATT_y
Cuts at: 1493 2393 4531 4757 4909 7018 1493
Size: 900 2138 226 152 2109 1697
Fragments arranged by size:
2138 2109 1697 900 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5776 160
Size: 872 4744 1606
Fragments arranged by size:
4744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
AvaI C'yCGr_G
Cuts at: 1870 1943 4547 1870
Size: 73 2604 4545
Fragments arranged by size:
4545 2604 73
BamHI G'GATC_C
Cuts at: 4552 4919 4552
Size: 367 6855
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2332 2987 4543 5998 618
Size: 325 217 627 545 655 1556 1455 1842
Fragments arranged by size:
1842 1556 1455 655 627 545 325 217
BanII G_rGCy'C
Cuts at: 1391 2493 3146 3219 4541 1391
Size: 1102 653 73 1322 4072
Fragments arranged by size:
4072 1322 1102 653 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 3649 928
Size: 971 930 820 4501
Fragments arranged by size:
4501 971 930 820
BbvCI CC'TCA_GC
Cuts at: 2835 2835
Size: 7222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5413 5447 1903
Size: 34 3476 34 3678
Fragments arranged by size:
3678 3476 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2758 5114 6324 2758
Size: 2356 1210 3656
Fragments arranged by size:
3656 2356 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7222
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5833 136
Size: 107 122 71 631 605 4161 1525
Fragments arranged by size:
4161 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2776 3070 3775 7217 2776
Size: 294 705 3442 2781
Fragments arranged by size:
3442 2781 705 294
BlpI GC'TnA_GC
Cuts at: 3597 4639 3597
Size: 1042 6180
Bme1580I G_kGCm'C
Cuts at: 1790 2528 3146 3153 3574 5283 6439 1790
Size: 738 618 7 421 1709 1156 2573
Fragments arranged by size:
2573 1709 1156 738 618 421 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 4953 5911
Size: 416 53 83 399 24 1730 1806 958
Cuts at: 5911 442
Size: 1753
Fragments arranged by size:
1806 1753 1730 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7222
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3072 4157 3072
Size: 1085 6137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3067 5882 1239
Size: 1828 2815 2579
Fragments arranged by size:
2815 2579 1828
Bpu10I CC'TnA_GC
Cuts at: 2653 2835 4288 2653
Size: 182 1453 5587
Fragments arranged by size:
5587 1453 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2245 3481 5347 6212 6510 2245
Size: 1236 1866 865 298 2957
Fragments arranged by size:
2957 1866 1236 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2507 3296 5885 882
Size: 617 1008 789 2589 2219
Fragments arranged by size:
2589 2219 1008 789 617
BsaAI yAC'GTr
Cuts at: 493 1204 3275 5013 493
Size: 711 2071 1738 2702
Fragments arranged by size:
2702 2071 1738 711
BsaBI GATnn'nnATC
Cuts at: 7216 7216
Size: 7222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 5029 5411 6046
Size: 53 83 186 936 608 2888 382 635
Cuts at: 6046 275
Size: 1451
Fragments arranged by size:
2888 1451 936 635 608 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5655 6053 6327 6474 6885 5655
Size: 398 274 147 411 5992
Fragments arranged by size:
5992 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6997
Fragments arranged by size:
6997 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3045 1186
Size: 1859 5363
BseYI C'CCAG_C
Cuts at: 3110 6425 6921 3110
Size: 3315 496 3411
Fragments arranged by size:
3411 3315 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3252 4062 4091 1163
Size: 573 1516 810 29 4294
Fragments arranged by size:
4294 1516 810 573 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4672 5433 6461 1540
Size: 275 186 2671 761 1028 2301
Fragments arranged by size:
2671 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 3574 4505 5283 5368 6439 2493
Size: 1081 931 778 85 1071 3276
Fragments arranged by size:
3276 1081 1071 931 778 85
BsmI GAATG_Cn'
Cuts at: 3722 4733 4826 3722
Size: 1011 93 6118
Fragments arranged by size:
6118 1011 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3662 3662
Size: 7222
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 2528 3146 3153 3219 3574 4505
Size: 399 703 35 618 7 66 355 931
Cuts at: 4505 4541 5283 5368 6439 1391
Size: 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 931 742 703 618 399 355
85 66 36 35 7
BspEI T'CCGG_A
Cuts at: 6885 6885
Size: 7222
BspHI T'CATG_A
Cuts at: 5111 6841 5111
Size: 1730 5492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2043 4577 844
Size: 1199 2534 3489
Fragments arranged by size:
3489 2534 1199
BsrBI CCG'CTC
Cuts at: 1394 1823 2315 5109 1394
Size: 429 492 2794 3507
Fragments arranged by size:
3507 2794 492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2566 4023 5717 5891 65
Size: 1183 1318 1457 1694 174 1396
Fragments arranged by size:
1694 1457 1396 1318 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2364 5866 6053 1700
Size: 110 554 3502 187 2869
Fragments arranged by size:
3502 2869 554 187 110
BsrGI T'GTAC_A
Cuts at: 96 2746 96
Size: 2650 4572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7222
BssSI C'ACGA_G
Cuts at: 3567 3734 5282 6294 3567
Size: 167 1548 1012 4495
Fragments arranged by size:
4495 1548 1012 167
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3275 4270 794
Size: 2481 995 3746
Fragments arranged by size:
3746 2481 995
BstBI TT'CG_AA
Cuts at: 6026 6026
Size: 7222
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7222
BstYI r'GATC_y
Cuts at: 1972 2776 3070 3775 4552 4919 5304 5321 6773
Size: 804 294 705 777 367 385 17 1452
Cuts at: 6773 6859 6871 7217 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 804 777 705 385 367 346
294 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3525 3525
Size: 7222
BtgI C'CryG_G
Cuts at: 513 1065 2004 3171 4245 513
Size: 552 939 1167 1074 3490
Fragments arranged by size:
3490 1167 1074 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2253 2326 3420 4455 487
Size: 683 56 1027 73 1094 1035 3254
Fragments arranged by size:
3254 1094 1035 1027 683 73 56
BtsI GCAGTG_nn'
Cuts at: 3655 3704 4738 5532 5552 3655
Size: 49 1034 794 20 5325
Fragments arranged by size:
5325 1034 794 49 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7187
DraI TTT'AAA
Cuts at: 2668 3804 4526 2668
Size: 1136 722 5364
Fragments arranged by size:
5364 1136 722
DraIII CAC_nnn'GTG
Cuts at: 3275 3275
Size: 7222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4442 6229 809
Size: 507 3126 1787 1802
Fragments arranged by size:
3126 1802 1787 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 3174 4669
Size: 54 1425 50 275 18 456 888 1495
Cuts at: 4669 5558 7187 8
Size: 889 1629 43
Fragments arranged by size:
1629 1495 1425 889 888 456 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4669 1537
Size: 275 2857 4090
Fragments arranged by size:
4090 2857 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 3063 3294 3438 3877 4425 5152 1353
Size: 1332 378 231 144 439 548 727 3423
Fragments arranged by size:
3423 1332 727 548 439 378 231 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5809 6183 6329 387
Size: 1064 138 435 3785 374 146 1280
Fragments arranged by size:
3785 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2491 2491
Size: 7222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2009 2088 2757 3067 3878 3887 4406
Size: 393 377 79 669 310 811 9 519
Cuts at: 4406 5285 5882 6669 6937 1239
Size: 879 597 787 268 1524
Fragments arranged by size:
1524 879 811 787 669 597 519 393
377 310 268 79 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2039 2039
Size: 7222
EcoO109I rG'GnC_Cy
Cuts at: 2032 2618 3142 3143 2032
Size: 586 524 1 6111
Fragments arranged by size:
6111 586 524 1
EcoRI G'AATT_C
Cuts at: 4531 4909 4531
Size: 378 6844
FspI TGC'GCA
Cuts at: 5728 5728
Size: 7222
HaeII r_GCGC'y
Cuts at: 1678 6369 1678
Size: 4691 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2105 2137 2710
Size: 32 135 32 488 32 13 32 573
Cuts at: 2710 2742 3119 3151 3193 3225 5863 5895 5937
Size: 32 377 32 42 32 2638 32 42
Cuts at: 5937 5969 1373
Size: 32 2626
Fragments arranged by size:
2638 2626 573 488 377 135 42 42
32 32 32 32 32 32 32 32
32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2139 3583 4566 4812 669
Size: 988 281 201 1444 983 246 3079
Fragments arranged by size:
3079 1444 988 983 281 246 201
HindIII A'AGCT_T
Cuts at: 748 3860 748
Size: 3112 4110
HpaI GTT'AAC
Cuts at: 4812 4812
Size: 7222
KpnI G_GTAC'C
Cuts at: 4547 4547
Size: 7222
MfeI C'AATT_G
Cuts at: 3761 4821 3761
Size: 1060 6162
MluI A'CGCG_T
Cuts at: 7098 7098
Size: 7222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 4046 6336 6520 1305
Size: 1155 1586 2290 184 2007
Fragments arranged by size:
2290 2007 1586 1155 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3176 10
Size: 54 1425 343 1344 4056
Fragments arranged by size:
4056 1425 1344 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 3225 3846 5180 5539 5698 518
Size: 648 2004 55 621 1334 359 159 2042
Fragments arranged by size:
2042 2004 1334 648 621 359 159 55
MspA1I CmG'CkG
Cuts at: 1606 3266 3770 3967 4383 4638 5315 6463 6708
Size: 1660 504 197 416 255 677 1148 245
Cuts at: 6708 1606
Size: 2120
Fragments arranged by size:
2120 1660 1148 677 504 416 255 245
197
NcoI C'CATG_G
Cuts at: 513 1065 2004 3171 4245 513
Size: 552 939 1167 1074 3490
Fragments arranged by size:
3490 1167 1074 939 552
NdeI CA'TA_TG
Cuts at: 387 2380 387
Size: 1993 5229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7222
NotI GC'GGCC_GC
Cuts at: 4669 4669
Size: 7222
NspI r_CATG'y
Cuts at: 1132 1229 3697 4580 6125 1132
Size: 97 2468 883 1545 2229
Fragments arranged by size:
2468 2229 1545 883 97
PasI CC'CwG_GG
Cuts at: 1348 2146 1348
Size: 798 6424
PciI A'CATG_T
Cuts at: 6121 6121
Size: 7222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2984 1108
Size: 1876 5346
PfoI T'CCnGG_A
Cuts at: 2843 2843
Size: 7222
PmeI GTTT'AAAC
Cuts at: 4526 4526
Size: 7222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5291 5323 6804 6836 5291
Size: 32 1481 32 5677
Fragments arranged by size:
5677 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2618 2032
Size: 586 6636
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7222
PsiI TTA'TAA
Cuts at: 4792 4792
Size: 7222
PspOMI G'GGCC_C
Cuts at: 3142 3142
Size: 7222
PstI C_TGCA'G
Cuts at: 830 2040 3775 4574 830
Size: 1210 1735 799 3478
Fragments arranged by size:
3478 1735 1210 799
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7222
PvuII CAG'CTG
Cuts at: 3770 3967 4383 3770
Size: 197 416 6609
Fragments arranged by size:
6609 416 197
SacI G_AGCT'C
Cuts at: 2493 2493
Size: 7222
SalI G'TCGA_C
Cuts at: 1936 4564 1936
Size: 2628 4594
SapI GCTCTTCn'nnn_
Cuts at: 4425 4425
Size: 7222
SbfI CC_TGCA'GG
Cuts at: 2040 4574 2040
Size: 2534 4688
ScaI AGT'ACT
Cuts at: 5470 7182 5470
Size: 1712 5510
SexAI A'CCwGG_T
Cuts at: 2950 2950
Size: 7222
SfcI C'TryA_G
Cuts at: 826 1045 2036 2071 3771 4570 5705 6386 6577
Size: 219 991 35 1700 799 1135 681 191
Cuts at: 6577 826
Size: 1471
Fragments arranged by size:
1700 1471 1135 991 799 681 219 191
35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7222
SmaI CCC'GGG
Cuts at: 1872 4549 1872
Size: 2677 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2224 3460 5326 6227 6489 820
Size: 197 926 281 1236 1866 901 262 1553
Fragments arranged by size:
1866 1553 1236 926 901 281 262 197
SnaBI TAC'GTA
Cuts at: 493 5013 493
Size: 4520 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7222
SphI G_CATG'C
Cuts at: 1132 4580 1132
Size: 3448 3774
SspI AAT'ATT
Cuts at: 5 52 5146 5
Size: 47 5094 2081
Fragments arranged by size:
5094 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7222
StyI C'CwwG_G
Cuts at: 513 1065 2004 3171 4245 4661 513
Size: 552 939 1167 1074 416 3074
Fragments arranged by size:
3074 1167 1074 939 552 416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5249 5266 5419 5604 6049 325
Size: 1255 3669 17 153 185 445 1498
Fragments arranged by size:
3669 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2746 2894 3508
Size: 274 80 33 51 1443 769 148 614
Cuts at: 3508 5468 7180 96
Size: 1960 1712 138
Fragments arranged by size:
1960 1712 1443 769 614 274 148 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2046 2078 7182 7214 2046
Size: 32 5104 32 2054
Fragments arranged by size:
5104 2054 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2117 1316
Size: 801 6421
XbaI T'CTAG_A
Cuts at: 3073 3211 3979 4558 3073
Size: 138 768 579 5737
Fragments arranged by size:
5737 768 579 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 3187 2803
Size: 384 6838
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7222
XmaI C'CCGG_G
Cuts at: 1870 4547 1870
Size: 2677 4545
XmnI GAAnn'nnTTC
Cuts at: 5351 5351
Size: 7222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5030 276
Size: 53 83 186 936 3496 2468
Fragments arranged by size:
3496 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI