Restriction map for
pFN21AB9189
(Circular) (Six-base) MAPSORT of: pFN21AB9189.seq Check: 6634 from: 1 to: 5992
pFN21AB9189 5992 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2108 3335 1937
Size: 171 1227 4594
Fragments arranged by size:
4594 1227 171
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 2413 4119 4492 1563
Size: 850 1706 373 3063
Fragments arranged by size:
3063 1706 850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 2306 2938 2945 4055 5439 5707 1632
Size: 617 57 632 7 1110 1384 268 1917
Fragments arranged by size:
1917 1384 1110 632 617 268 57 7
AflII C'TTAA_G
Cuts at: 820 1017 3071 820
Size: 197 2054 3741
Fragments arranged by size:
3741 2054 197
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 4721 4721
Size: 5992
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 2171
Size: 32 5960
AlwNI CAG_nnn'CTG
Cuts at: 3735 5307 3735
Size: 1572 4420
ApaI G_GGCC'C
Cuts at: 3247 3247
Size: 5992
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 2198 2588 2990 3301 3527 3679 5788 1493
Size: 705 390 402 311 226 152 2109 1697
Fragments arranged by size:
2109 1697 705 402 390 311 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 1870
Size: 73 1374 4545
Fragments arranged by size:
4545 1374 73
BamHI G'GATC_C
Cuts at: 2442 3322 3689 2442
Size: 880 367 4745
Fragments arranged by size:
4745 880 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3313 4768 618
Size: 325 217 627 1526 1455 1842
Fragments arranged by size:
1842 1526 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3247 3311 1391
Size: 1856 64 4072
Fragments arranged by size:
4072 1856 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2951 3156 928
Size: 971 1052 205 3764
Fragments arranged by size:
3764 1052 971 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4603 136
Size: 107 122 71 631 605 2931 1525
Fragments arranged by size:
2931 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 3247 4053 5209 1790
Size: 1457 806 1156 2573
Fragments arranged by size:
2573 1457 1156 806
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2368 3723 4681
Size: 416 53 83 399 24 951 1355 958
Cuts at: 4681 442
Size: 1753
Fragments arranged by size:
1753 1355 958 951 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4652 1239
Size: 3413 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2100 3188 4117 4982 5280 2100
Size: 1088 929 865 298 2812
Fragments arranged by size:
2812 1088 929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 2313 4655 882
Size: 617 518 296 2342 2219
Fragments arranged by size:
2342 2219 617 518 296
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 2834 5986 2834
Size: 3152 2840
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3799 4181 4816 275
Size: 53 83 186 936 2266 382 635 1451
Fragments arranged by size:
2266 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5767
Fragments arranged by size:
5767 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3185 3188 1186
Size: 1999 3 3990
Fragments arranged by size:
3990 1999 3
BseYI C'CCAG_C
Cuts at: 2212 3231 3246 5195 5691 2212
Size: 1019 15 1949 496 2513
Fragments arranged by size:
2513 1949 1019 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2567 2804 1163
Size: 573 831 237 4351
Fragments arranged by size:
4351 831 573 237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4053 4138 5209 4053
Size: 85 1071 4836
Fragments arranged by size:
4836 1071 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3247 3311 4053 4138 5209 1391
Size: 399 1457 64 742 85 1071 2174
Fragments arranged by size:
2174 1457 1071 742 399 85 64
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 2604 3881 5611 2604
Size: 1277 1730 2985
Fragments arranged by size:
2985 1730 1277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 2776 3347 844
Size: 1553 379 571 3489
Fragments arranged by size:
3489 1553 571 379
BsrBI CCG'CTC
Cuts at: 1394 1823 3879 1394
Size: 429 2056 3507
Fragments arranged by size:
3507 2056 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2281 2565 2629 2803 4487 4661 65
Size: 1183 1033 284 64 174 1684 174 1396
Fragments arranged by size:
1684 1396 1183 1033 284 174 174 64
BsrFI r'CCGG_y
Cuts at: 1700 1810 4636 4823 1700
Size: 110 2826 187 2869
Fragments arranged by size:
2869 2826 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 2437 3095 4052 5064 2437
Size: 658 957 1012 3365
Fragments arranged by size:
3365 1012 957 658
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5992
BstBI TT'CG_AA
Cuts at: 2044 4796 2044
Size: 2752 3240
BstEII G'GTnAC_C
Cuts at: 1184 2503 1184
Size: 1319 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2176 3239 2011
Size: 165 1063 4764
Fragments arranged by size:
4764 1063 165
BstYI r'GATC_y
Cuts at: 1972 2442 2497 3322 3689 4074 4091 5543 5629
Size: 470 55 825 367 385 17 1452 86
Cuts at: 5629 5641 5987 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 825 470 385 367 346 86
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2016 2307 513
Size: 552 939 6 6 291 4198
Fragments arranged by size:
4198 939 552 291 6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5253
Fragments arranged by size:
5253 683 56
BtsI GCAGTG_nn'
Cuts at: 2715 3508 4302 4322 2715
Size: 793 794 20 4385
Fragments arranged by size:
4385 794 793 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2096 4999 809
Size: 507 780 2903 1802
Fragments arranged by size:
2903 1802 780 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2013 2062
Size: 54 1425 50 275 18 177 6 49
Cuts at: 2062 3439 4328 5957 8
Size: 1377 889 1629 43
Fragments arranged by size:
1629 1425 1377 889 275 177 54 50
49 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2426 2721 2907 3922 1353
Size: 678 395 295 186 1015 3423
Fragments arranged by size:
3423 1015 678 395 295 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4579 4953 5099 387
Size: 1064 138 2990 374 146 1280
Fragments arranged by size:
2990 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2306 2938 2945 4055 4652 5439
Size: 393 617 57 632 7 1110 597 787
Cuts at: 5439 5707 1239
Size: 268 1524
Fragments arranged by size:
1524 1110 787 632 617 597 393 268
57 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2393 2393
Size: 5992
EcoO109I rG'GnC_Cy
Cuts at: 3243 3243
Size: 5992
EcoRI G'AATT_C
Cuts at: 2990 3301 3679 2990
Size: 311 378 5303
Fragments arranged by size:
5303 378 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3086 3086
Size: 5992
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 5139 1678
Size: 3461 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2680 2712 4633 4665 4707
Size: 32 135 32 1108 32 1921 32 42
Cuts at: 4707 4739 1373
Size: 32 2626
Fragments arranged by size:
2626 1921 1108 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2101 2292 2302 3336 3582 669
Size: 988 281 163 191 10 1034 246 3079
Fragments arranged by size:
3079 1034 988 281 246 191 163 10
HindIII A'AGCT_T
Cuts at: 748 2906 3283 748
Size: 2158 377 3457
Fragments arranged by size:
3457 2158 377
HpaI GTT'AAC
Cuts at: 2292 3582 2292
Size: 1290 4702
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5106 5290 1305
Size: 3801 184 2007
Fragments arranged by size:
3801 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 10
Size: 54 1425 343 232 3938
Fragments arranged by size:
3938 1425 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2174 3950 4309 4468 518
Size: 648 843 165 1776 359 159 2042
Fragments arranged by size:
2042 1776 843 648 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 2095 3206 3408 4085 5233 5478 1606
Size: 489 1111 202 677 1148 245 2120
Fragments arranged by size:
2120 1148 1111 677 489 245 202
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4501
Fragments arranged by size:
4501 939 552
NdeI CA'TA_TG
Cuts at: 387 2971 387
Size: 2584 3408
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 3350 4895 1132
Size: 97 2121 1545 2229
Fragments arranged by size:
2229 2121 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2772 1348
Size: 1424 4568
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 4061 4093 5574 5606 2906
Size: 32 1123 32 1481 32 3292
Fragments arranged by size:
3292 1481 1123 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PspOMI G'GGCC_C
Cuts at: 3243 3243
Size: 5992
PstI C_TGCA'G
Cuts at: 830 3009 3344 830
Size: 2179 335 3478
Fragments arranged by size:
3478 2179 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2095 2095
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SapI GCTCTTCn'nnn_
Cuts at: 2031 2031
Size: 5992
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SexAI A'CCwGG_T
Cuts at: 2230 2230
Size: 5992
SfcI C'TryA_G
Cuts at: 826 1045 3005 3218 3340 4475 5156 5347 826
Size: 219 1960 213 122 1135 681 191 1471
Fragments arranged by size:
1960 1471 1135 681 219 213 191 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2115 3071 3167 4096 4997 5259
Size: 197 926 172 956 96 929 901 262
Cuts at: 5259 820
Size: 1553
Fragments arranged by size:
1553 956 929 926 901 262 197 172
96
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 2384 1517
Size: 867 5125
StyI C'CwwG_G
Cuts at: 513 1065 2004 3431 513
Size: 552 939 1427 3074
Fragments arranged by size:
3074 1427 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4019 4036 4189 4374 4819 325
Size: 1255 2439 17 153 185 445 1498
Fragments arranged by size:
2439 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4238 5950 96
Size: 274 80 33 51 1443 2261 1712 138
Fragments arranged by size:
2261 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5952 5984 5952
Size: 32 5960
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2515 2515
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 2990 3185 4121 2040
Size: 950 195 936 3911
Fragments arranged by size:
3911 950 936 195
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI