Restriction map for
pFN21AB9185
(Circular) (Six-base) MAPSORT of: pFN21AB9185.seq Check: 8815 from: 1 to: 5833
pFN21AB9185 5833 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2055 2622 2055
Size: 567 5266
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 2683 2956 3896 5280 5548 1632
Size: 413 638 273 940 1384 268 1917
Fragments arranged by size:
1917 1384 940 638 413 273 268
AfeI AGC'GCT
Cuts at: 2255 2336 2255
Size: 81 5752
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 2508 2760 4732 5709 1203
Size: 1305 252 1972 977 1327
Fragments arranged by size:
1972 1327 1305 977 252
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2668 1184
Size: 1484 4349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 2924 2956 2414
Size: 32 478 32 5291
Fragments arranged by size:
5291 478 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3576 5148 3576
Size: 1572 4261
ApaLI G'TGCA_C
Cuts at: 2104 3890 5046 2104
Size: 1786 1156 2891
Fragments arranged by size:
2891 1786 1156
ApoI r'AATT_y
Cuts at: 1493 3142 3368 3520 5629 1493
Size: 1649 226 152 2109 1697
Fragments arranged by size:
2109 1697 1649 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2501 3158 1870
Size: 73 558 657 4545
Fragments arranged by size:
4545 657 558 73
BamHI G'GATC_C
Cuts at: 2730 3163 3530 2730
Size: 433 367 5033
Fragments arranged by size:
5033 433 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2484 3154 4609 618
Size: 325 217 627 697 670 1455 1842
Fragments arranged by size:
1842 1455 697 670 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2357 3152 1391
Size: 966 795 4072
Fragments arranged by size:
4072 966 795
BbeI G_GCGC'C
Cuts at: 2488 2488
Size: 5833
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2033 2056 2793 928
Size: 971 134 23 737 3968
Fragments arranged by size:
3968 971 737 134 23
BbvCI CC'TCA_GC
Cuts at: 2480 2480
Size: 5833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2394 3894 5050 1790
Size: 318 286 1500 1156 2573
Fragments arranged by size:
2573 1500 1156 318 286
BmgBI CAC'GTC
Cuts at: 1318 2409 1318
Size: 1091 4742
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 3564 4522
Size: 416 53 83 399 24 609 1538 958
Cuts at: 4522 442
Size: 1753
Fragments arranged by size:
1753 1538 958 609 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2796 2796
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2197 2219 2441 2840 3082 4493 1239
Size: 958 22 222 399 242 1411 2579
Fragments arranged by size:
2579 1411 958 399 242 222 22
Bpu10I CC'TnA_GC
Cuts at: 2480 2586 2480
Size: 106 5727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2588 3958 4823 5121 2588
Size: 1370 865 298 3300
Fragments arranged by size:
3300 1370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2543 4496 882
Size: 617 1044 1953 2219
Fragments arranged by size:
2219 1953 1044 617
BsaAI yAC'GTr
Cuts at: 493 1204 2389 3624 493
Size: 711 1185 1235 2702
Fragments arranged by size:
2702 1235 1185 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2485 2988 3640 4022
Size: 53 83 186 936 952 503 652 382
Cuts at: 4022 4657 275
Size: 635 1451
Fragments arranged by size:
1451 952 936 652 635 503 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 2414 2444 1178
Size: 30 165 30 789 30 192 30 4567
Fragments arranged by size:
4567 789 192 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 2785 1186
Size: 1279 320 4234
Fragments arranged by size:
4234 1279 320
BseYI C'CCAG_C
Cuts at: 2347 2725 5036 5532 2347
Size: 378 2311 496 2648
Fragments arranged by size:
2648 2311 496 378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2027 2197 1163
Size: 573 291 170 4799
Fragments arranged by size:
4799 573 291 170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2271 2357 3894 3979 5050 2108
Size: 163 86 1537 85 1071 2891
Fragments arranged by size:
2891 1537 1071 163 86 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2271 2357 2394 3152 3894 3979
Size: 399 318 163 86 37 758 742 85
Cuts at: 3979 5050 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 758 742 399 318 163 86
85 37
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 2622 3188 844
Size: 1211 567 566 3489
Fragments arranged by size:
3489 1211 567 566
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2122 4477 4664 1700
Size: 110 312 2355 187 2869
Fragments arranged by size:
2869 2355 312 187 110
BsrGI T'GTAC_A
Cuts at: 96 2222 96
Size: 2126 3707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2730 3163 3530 3915 3932 5384 5470 5482
Size: 758 433 367 385 17 1452 86 12
Cuts at: 5482 5828 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 758 433 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2244 2466 2621 2918 513
Size: 552 939 240 222 155 297 3428
Fragments arranged by size:
3428 939 552 297 240 222 155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DraIII CAC_nnn'GTG
Cuts at: 2220 2220
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2468 4840 809
Size: 507 1152 2372 1802
Fragments arranged by size:
2372 1802 1152 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2120 3280 4169
Size: 54 1425 50 275 18 290 1160 889
Cuts at: 4169 5798 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1160 889 290 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2975 3763 1353
Size: 1622 788 3423
Fragments arranged by size:
3423 1622 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2771 4420 4794 4940 387
Size: 1064 138 1182 1649 374 146 1280
Fragments arranged by size:
1649 1280 1182 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2355 2355
Size: 5833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2197 2219 2441 2683 2840 2956
Size: 393 413 152 22 222 242 157 116
Cuts at: 2956 3082 3896 4493 5280 5548 1239
Size: 126 814 597 787 268 1524
Fragments arranged by size:
1524 814 787 597 413 393 268 242
222 157 152 126 116 22
EcoO109I rG'GnC_Cy
Cuts at: 2362 2362
Size: 5833
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2117 2257 2338 2488 4980 1678
Size: 439 140 81 150 2492 2531
Fragments arranged by size:
2531 2492 439 150 140 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2854 2886 2994
Size: 32 135 32 620 32 630 32 108
Cuts at: 2994 3026 4474 4506 4548 4580 1373
Size: 32 1448 32 42 32 2626
Fragments arranged by size:
2626 1448 630 620 135 108 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 2484 2484
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 4947 5131 1305
Size: 1167 2475 184 2007
Fragments arranged by size:
2475 2007 1167 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2573 2765 3791 4150 4309 518
Size: 648 1407 192 1026 359 159 2042
Fragments arranged by size:
2042 1407 1026 648 359 192 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2623 2675 2797 3000 3249 3926 5074
Size: 495 522 52 122 203 249 677 1148
Cuts at: 5074 5319 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 522 495 249 245 203
122 52
NarI GG'CG_CC
Cuts at: 2485 2485
Size: 5833
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4342
Fragments arranged by size:
4342 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2512 2764 2884 3191 4736 1132
Size: 97 1283 252 120 307 1545 2229
Fragments arranged by size:
2229 1545 1283 307 252 120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 2508 2760 4732 2508
Size: 252 1972 3609
Fragments arranged by size:
3609 1972 252
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PfoI T'CCnGG_A
Cuts at: 2856 2856
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2414 2446 3902 3934 5415 5447 2414
Size: 32 1456 32 1481 32 2800
Fragments arranged by size:
2800 1481 1456 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 3185 830
Size: 2355 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2101 2675 2101
Size: 574 5259
SacI G_AGCT'C
Cuts at: 2357 2357
Size: 5833
SacII CC_GC'GG
Cuts at: 2624 2624
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 3181 4316 4997 5188 826
Size: 219 2136 1135 681 191 1471
Fragments arranged by size:
2136 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2486 2486
Size: 5833
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2603 3937 4838 5100 820
Size: 197 926 660 1334 901 262 1553
Fragments arranged by size:
1553 1334 926 901 660 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 2628 3272 513
Size: 552 939 354 270 644 3074
Fragments arranged by size:
3074 939 644 552 354 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2307 3860 3877 4030 4215 4660 325
Size: 1255 727 1553 17 153 185 445 1498
Fragments arranged by size:
1553 1498 1255 727 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2222 2505 2523
Size: 274 80 33 51 1443 245 283 18
Cuts at: 2523 4079 5791 96
Size: 1556 1712 138
Fragments arranged by size:
1712 1556 1443 283 274 245 138 80
51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2314 2346 5793 5825 2314
Size: 32 3447 32 2322
Fragments arranged by size:
3447 2322 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI