Restriction map for
pFN21AB9166
(Circular) (Six-base) MAPSORT of: pFN21AB9166.seq Check: 2768 from: 1 to: 10714
pFN21AB9166 10714 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2132 2756 2909 3005 3119 3296 4010 4160 4274
Size: 624 153 96 114 177 714 150 114
Cuts at: 4274 4844 5261 2132
Size: 570 417 7585
Fragments arranged by size:
7585 714 624 570 417 177 153 150
114 114 96
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4695 7422 8524 278
Size: 53 83 186 936 3159 2727 1102 2468
Fragments arranged by size:
3159 2727 2468 1102 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4728 6424 7656 8057 1937
Size: 2791 1696 1232 401 4594
Fragments arranged by size:
4594 2791 1696 1232 401
Acc65I G'GTAC_C
Cuts at: 4343 8035 4343
Size: 3692 7022
AclI AA'CG_TT
Cuts at: 1563 8841 9214 1563
Size: 7278 373 3063
Fragments arranged by size:
7278 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5730 5883 6066 6566 6645 8777 10161 10429
Size: 4098 153 183 500 79 2132 1384 268
Cuts at: 10429 1632
Size: 1917
Fragments arranged by size:
4098 2132 1917 1384 500 268 183 153
79
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10517
AflIII A'CryG_T
Cuts at: 1203 3186 9613 10590 1203
Size: 1983 6427 977 1327
Fragments arranged by size:
6427 1983 1327 977
AgeI A'CCGG_T
Cuts at: 9545 9545
Size: 10714
AhdI GACnn_n'nnGTC
Cuts at: 4200 9443 4200
Size: 5243 5471
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10714
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2731 3638 5958 6942 7242 1184
Size: 1547 907 2320 984 300 4656
Fragments arranged by size:
4656 2320 1547 984 907 300
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4939 4971 4939
Size: 32 10682
AlwNI CAG_nnn'CTG
Cuts at: 3301 4446 4534 5063 5107 5938 6112 6596 7060
Size: 1145 88 529 44 831 174 484 464
Cuts at: 7060 7360 8457 10029 3301
Size: 300 1097 1572 3986
Fragments arranged by size:
3986 1572 1145 1097 831 529 484 464
300 174 88 44
ApaI G_GGCC'C
Cuts at: 3285 4290 4403 4596 5269 5468 5508 5889 6040
Size: 1005 113 193 673 199 40 381 151
Cuts at: 6040 7521 3285
Size: 1481 6478
Fragments arranged by size:
6478 1481 1005 673 381 199 193 151
113 40
ApaLI G'TGCA_C
Cuts at: 2311 8771 9927 2311
Size: 6460 1156 3098
Fragments arranged by size:
6460 3098 1156
ApoI r'AATT_y
Cuts at: 1493 8023 8249 8401 10510 1493
Size: 6530 226 152 2109 1697
Fragments arranged by size:
6530 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9268 160
Size: 872 8236 1606
Fragments arranged by size:
8236 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10714
AvaI C'yCGr_G
Cuts at: 1870 1943 2416 3100 5584 5952 6039 6066 7041
Size: 73 473 684 2484 368 87 27 975
Cuts at: 7041 7245 7380 7705 8039 1870
Size: 204 135 325 334 4545
Fragments arranged by size:
4545 2484 975 684 473 368 334 325
204 135 87 73 27
AvrII C'CTAG_G
Cuts at: 2266 2723 6388 2266
Size: 457 3665 6592
Fragments arranged by size:
6592 3665 457
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4335 4368 4335
Size: 33 10681
BamHI G'GATC_C
Cuts at: 3104 8044 8411 3104
Size: 4940 367 5407
Fragments arranged by size:
5407 4940 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2627 2744 2993 3638
Size: 325 217 627 333 507 117 249 645
Cuts at: 3638 4343 4364 8035 9490 618
Size: 705 21 3671 1455 1842
Fragments arranged by size:
3671 1842 1455 705 645 627 507 333
325 249 217 117 21
BanII G_rGCy'C
Cuts at: 1391 2274 2654 3131 3285 4290 4403 4596 5269
Size: 883 380 477 154 1005 113 193 673
Cuts at: 5269 5468 5508 5889 5918 6040 6265 6273 6397
Size: 199 40 381 29 122 225 8 124
Cuts at: 6397 6445 6849 7521 7576 8033 1391
Size: 48 404 672 55 457 4072
Fragments arranged by size:
4072 1005 883 673 672 477 457 404
381 380 225 199 193 154 124 122
113 55 48 40 29 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2367 3189 7152 928
Size: 971 468 822 3963 4490
Fragments arranged by size:
4490 3963 971 822 468
BbvCI CC'TCA_GC
Cuts at: 3559 3559
Size: 10714
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2552 2586 5466 5500 7268
Size: 34 50 34 531 34 2880 34 1768
Cuts at: 7268 7302 8905 8939 1903
Size: 34 1603 34 3678
Fragments arranged by size:
3678 2880 1768 1603 531 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8606 9816 8606
Size: 1210 9504
BclI T'GATC_A
Cuts at: 1548 3427 5785 1548
Size: 1879 2358 6477
Fragments arranged by size:
6477 2358 1879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2325 2556 2625
Size: 107 122 71 631 605 653 231 69
Cuts at: 2625 3513 4161 4251 4506 4992 5358 6392 7136
Size: 888 648 90 255 486 366 1034 744
Cuts at: 7136 9325 136
Size: 2189 1525
Fragments arranged by size:
2189 1525 1034 888 744 653 648 631
605 486 366 255 231 122 107 90
71 69
BglII A'GATC_T
Cuts at: 10709 10709
Size: 10714
BlpI GC'TnA_GC
Cuts at: 8131 8131
Size: 10714
Bme1580I G_kGCm'C
Cuts at: 1790 2167 2315 2747 2873 2996 3100 3285 3982
Size: 377 148 432 126 123 104 185 697
Cuts at: 3982 4017 4108 4290 4403 4552 4596 4876 5269
Size: 35 91 182 113 149 44 280 393
Cuts at: 5269 5468 5508 5805 5889 6040 6902 7521 7557
Size: 199 40 297 84 151 862 619 36
Cuts at: 7557 8775 9931 1790
Size: 1218 1156 2573
Fragments arranged by size:
2573 1218 1156 862 697 619 432 393
377 297 280 199 185 182 151 149
148 126 123 113 104 91 84 44
40 36 35
BmgBI CAC'GTC
Cuts at: 1318 2187 4045 5023 7183 1318
Size: 869 1858 978 2160 4849
Fragments arranged by size:
4849 2160 1858 978 869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4285 4599 4845
Size: 416 53 83 399 24 2868 314 246
Cuts at: 4845 5605 6356 6912 7231 7450 8445 9403 442
Size: 760 751 556 319 219 995 958 1753
Fragments arranged by size:
2868 1753 995 958 760 751 556 416
399 319 314 246 219 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 7409 1056
Size: 6353 4361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2154 2788 3460 3574 6145 2154
Size: 634 672 114 2571 6723
Fragments arranged by size:
6723 2571 672 634 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3735 4269 4568 4601 4974 4986 5484 5733
Size: 2496 534 299 33 373 12 498 249
Cuts at: 5733 5745 6287 6392 6993 7250 9374 1239
Size: 12 542 105 601 257 2124 2579
Fragments arranged by size:
2579 2496 2124 601 542 534 498 373
299 257 249 105 33 12 12
Bpu10I CC'TnA_GC
Cuts at: 2129 3559 5322 6589 6843 2129
Size: 1430 1763 1267 254 6000
Fragments arranged by size:
6000 1763 1430 1267 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6521 6986 8839 9704 10002 6521
Size: 465 1853 865 298 7233
Fragments arranged by size:
7233 1853 865 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3536 5441 5735 6116 6539 6666 6902
Size: 617 2037 1905 294 381 423 127 236
Cuts at: 6902 7840 9377 882
Size: 938 1537 2219
Fragments arranged by size:
2219 2037 1905 1537 938 617 423 381
294 236 127
BsaAI yAC'GTr
Cuts at: 493 1204 4795 6648 8505 493
Size: 711 3591 1853 1857 2702
Fragments arranged by size:
3591 2702 1857 1853 711
BsaBI GATnn'nnATC
Cuts at: 10708 10708
Size: 10714
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4692 6421 6427 6502
Size: 53 83 186 936 3159 1729 6 75
Cuts at: 6502 7310 7419 8521 8903 9538 275
Size: 808 109 1102 382 635 1451
Fragments arranged by size:
3159 1729 1451 1102 936 808 635 382
186 109 83 75 53 6
BsaWI w'CCGG_w
Cuts at: 4514 9147 9545 9819 9966 10377 4514
Size: 4633 398 274 147 411 4851
Fragments arranged by size:
4851 4633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3395 3425 4255 4285 4939
Size: 30 165 30 1992 30 830 30 654
Cuts at: 4939 4969 6597 6627 7818 7848 1178
Size: 30 1628 30 1191 30 4044
Fragments arranged by size:
4044 1992 1628 1191 830 654 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3077 3665 4202 6614 6623 6699 7737 8013
Size: 1891 588 537 2412 9 76 1038 276
Cuts at: 8013 1186
Size: 3887
Fragments arranged by size:
3887 2412 1891 1038 588 537 276 76
9
BseYI C'CCAG_C
Cuts at: 2217 2276 2300 2352 2928 3042 3321 3447 4128
Size: 59 24 52 576 114 279 126 681
Cuts at: 4128 4427 4845 5838 5920 6140 7353 7363 7369
Size: 299 418 993 82 220 1213 10 6
Cuts at: 7369 7400 7469 7514 7966 9917 10413 2217
Size: 31 69 45 452 1951 496 2518
Fragments arranged by size:
2518 1951 1213 993 681 576 496 452
418 299 279 220 126 114 82 69
59 52 45 31 24 10 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3688 3982 4080 4132 4246 4455 4656
Size: 573 1952 294 98 52 114 209 201
Cuts at: 4656 5740 6902 7202 1163
Size: 1084 1162 300 4675
Fragments arranged by size:
4675 1952 1162 1084 573 300 294 209
201 114 98 52
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7744 8164 8925 9953 1540
Size: 275 186 5743 420 761 1028 2301
Fragments arranged by size:
5743 2301 1028 761 420 275 186
BsiHKAI G_wGCw'C
Cuts at: 2315 2435 3895 6359 6445 6590 8775 8860 9931
Size: 120 1460 2464 86 145 2185 85 1071
Cuts at: 9931 2315
Size: 3098
Fragments arranged by size:
3098 2464 2185 1460 1071 145 120 86
85
BsmI GAATG_Cn'
Cuts at: 8225 8318 8225
Size: 93 10621
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 4699 5013 2177
Size: 2522 314 7878
Fragments arranged by size:
7878 2522 314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2167 2274 2315 2435 2654 2747 2873
Size: 399 377 107 41 120 219 93 126
Cuts at: 2873 2996 3100 3131 3285 3895 3982 4017 4108
Size: 123 104 31 154 610 87 35 91
Cuts at: 4108 4290 4403 4552 4596 4876 5269 5468 5508
Size: 182 113 149 44 280 393 199 40
Cuts at: 5508 5805 5889 5918 6040 6265 6273 6359 6397
Size: 297 84 29 122 225 8 86 38
Cuts at: 6397 6445 6590 6849 6902 7521 7557 7576 8033
Size: 48 145 259 53 619 36 19 457
Cuts at: 8033 8775 8860 9931 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 610 457 399 393
377 297 280 259 225 219 199 182
154 149 145 126 123 122 120 113
107 104 93 91 87 86 85 84
53 48 44 41 40 38 36 35
31 29 19 8
BspEI T'CCGG_A
Cuts at: 10377 10377
Size: 10714
BspHI T'CATG_A
Cuts at: 8603 10333 8603
Size: 1730 8984
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2132 2162 2756 2909 3005 3119 3296 3603
Size: 1288 30 594 153 96 114 177 307
Cuts at: 3603 4010 4127 4160 4274 4472 4844 5261 8069
Size: 407 117 33 114 198 372 417 2808
Cuts at: 8069 844
Size: 3489
Fragments arranged by size:
3489 2808 1288 594 417 407 372 307
198 177 153 117 114 114 96 33
30
BsrBI CCG'CTC
Cuts at: 1394 1823 2261 7257 7340 7936 8601 1394
Size: 429 438 4996 83 596 665 3507
Fragments arranged by size:
4996 3507 665 596 438 429 83
BsrDI GCAATG_nn'
Cuts at: 65 1248 7690 9209 9383 65
Size: 1183 6442 1519 174 1396
Fragments arranged by size:
6442 1519 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6504 7411 9358 9545 1700
Size: 110 4694 907 1947 187 2869
Fragments arranged by size:
4694 2869 1947 907 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10714
BssHII G'CGCG_C
Cuts at: 1925 7528 1925
Size: 5603 5111
BssSI C'ACGA_G
Cuts at: 7614 8774 9786 7614
Size: 1160 1012 8542
Fragments arranged by size:
8542 1160 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2634 4103 4782 6326 794
Size: 1840 1469 679 1544 5182
Fragments arranged by size:
5182 1840 1544 1469 679
BstBI TT'CG_AA
Cuts at: 2735 9518 2735
Size: 6783 3931
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10714
BstXI CCAn_nnnn'nTGG
Cuts at: 2919 4136 4840 4853 5458 5634 2919
Size: 1217 704 13 605 176 7999
Fragments arranged by size:
7999 1217 704 605 176 13
BstYI r'GATC_y
Cuts at: 1972 2535 3104 4025 7444 8044 8411 8796 8813
Size: 563 569 921 3419 600 367 385 17
Cuts at: 8813 10265 10351 10363 10709 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3419 1977 1452 921 600 569 563 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3987 4479 6597 6690 3987
Size: 492 2118 93 8011
Fragments arranged by size:
8011 2118 492 93
BtgI C'CryG_G
Cuts at: 513 1065 3208 4009 4108 4386 4942 5296 5451
Size: 552 2143 801 99 278 556 354 155
Cuts at: 5451 6751 6894 513
Size: 1300 143 4333
Fragments arranged by size:
4333 2143 1300 801 556 552 354 278
155 143 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5580 6260 487
Size: 683 56 4354 680 4941
Fragments arranged by size:
4941 4354 683 680 56
BtsI GCAGTG_nn'
Cuts at: 2018 2468 2545 2977 3022 3496 3704 3724 4015
Size: 450 77 432 45 474 208 20 291
Cuts at: 4015 4576 4603 4753 5061 5224 5287 5584 5693
Size: 561 27 150 308 163 63 297 109
Cuts at: 5693 5823 6261 6287 7089 8230 9024 9044 2018
Size: 130 438 26 802 1141 794 20 3688
Fragments arranged by size:
3688 1141 802 794 561 474 450 438
432 308 297 291 208 163 150 130
109 77 63 45 27 26 20 20
ClaI AT'CG_AT
Cuts at: 1554 3660 1554
Size: 2106 8608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3097 3132 3566 3601 529
Size: 35 2533 35 434 35 7642
Fragments arranged by size:
7642 2533 434 35 35 35
DraI TTT'AAA
Cuts at: 8018 8018
Size: 10714
DraIII CAC_nnn'GTG
Cuts at: 2696 3028 4103 5345 5702 6895 2696
Size: 332 1075 1242 357 1193 6515
Fragments arranged by size:
6515 1242 1193 1075 357 332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2843 9721 809
Size: 507 1527 6878 1802
Fragments arranged by size:
6878 1802 1527 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2520 5082 6366
Size: 54 1425 50 275 18 690 2562 1284
Cuts at: 6366 6941 7741 8161 9050 10679 8
Size: 575 800 420 889 1629 43
Fragments arranged by size:
2562 1629 1425 1284 889 800 690 575
420 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7741 8161 1537
Size: 275 5929 420 4090
Fragments arranged by size:
5929 4090 420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4702 5678 5706 6402 8644 1353
Size: 3349 976 28 696 2242 3423
Fragments arranged by size:
3423 3349 2242 976 696 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4412 7430 7638 9301 9675 9821
Size: 1064 138 2823 3018 208 1663 374 146
Cuts at: 9821 387
Size: 1280
Fragments arranged by size:
3018 2823 1663 1280 1064 374 208 146
138
EcoICRI GAG'CTC
Cuts at: 6443 6443
Size: 10714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3735 4269 4568 4601 4974 4986 5484
Size: 393 2103 534 299 33 373 12 498
Cuts at: 5484 5730 5733 5745 5883 6066 6287 6392 6566
Size: 246 3 12 138 183 221 105 174
Cuts at: 6566 6645 6993 7250 8777 9374 10161 10429 1239
Size: 79 348 257 1527 597 787 268 1524
Fragments arranged by size:
2103 1527 1524 787 597 534 498 393
373 348 299 268 257 246 221 183
174 138 105 79 33 12 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 4784 5325 6116 6309 6965 4784
Size: 541 791 193 656 8533
Fragments arranged by size:
8533 791 656 541 193
EcoO109I rG'GnC_Cy
Cuts at: 2151 2605 2612 2711 2969 3281 3298 4116 4172
Size: 454 7 99 258 312 17 818 56
Cuts at: 4172 4286 4287 4399 4484 4593 5181 5246 5265
Size: 114 1 112 85 109 588 65 19
Cuts at: 5265 5266 5369 5464 5504 5505 5559 5872 5885
Size: 1 103 95 40 1 54 313 13
Cuts at: 5885 5886 6036 6070 6214 6593 6813 6873 7518
Size: 1 150 34 144 379 220 60 645
Cuts at: 7518 7610 7680 2151
Size: 92 70 5185
Fragments arranged by size:
5185 818 645 588 454 379 313 312
258 220 150 144 114 112 109 103
99 95 92 85 70 65 60 56
54 40 34 19 17 13 7 1
1 1 1
EcoRI G'AATT_C
Cuts at: 8023 8401 8023
Size: 378 10336
EcoRV GAT'ATC
Cuts at: 3658 3658
Size: 10714
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2270 5347 2270
Size: 3077 7637
FspI TGC'GCA
Cuts at: 9220 9220
Size: 10714
HaeII r_GCGC'y
Cuts at: 1678 6686 7412 9861 1678
Size: 5008 726 2449 2531
Fragments arranged by size:
5008 2531 2449 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4186 4218 4618 4650 7818
Size: 32 135 32 2614 32 400 32 3168
Cuts at: 7818 7850 7894 7925 7926 7957 9355 9387 9429
Size: 32 44 31 1 31 1398 32 42
Cuts at: 9429 9461 1373
Size: 32 2626
Fragments arranged by size:
3168 2626 2614 1398 400 135 44 42
32 32 32 32 32 32 32 31
31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2590 4729 6425 8058 8304 669
Size: 988 281 652 2139 1696 1633 246 3079
Fragments arranged by size:
3079 2139 1696 1633 988 652 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10714
HpaI GTT'AAC
Cuts at: 8304 8304
Size: 10714
KpnI G_GTAC'C
Cuts at: 4347 8039 4347
Size: 3692 7022
MfeI C'AATT_G
Cuts at: 8313 8313
Size: 10714
MluI A'CGCG_T
Cuts at: 10590 10590
Size: 10714
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3784 4031 7917 9828 10012 1305
Size: 2479 247 3886 1911 184 2007
Fragments arranged by size:
3886 2479 2007 1911 247 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2522 5084 10
Size: 54 1425 343 690 2562 5640
Fragments arranged by size:
5640 2562 1425 690 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 3213 4036 4838 4941 6306 8672
Size: 648 1408 639 823 802 103 1365 2366
Cuts at: 8672 9031 9190 518
Size: 359 159 2042
Fragments arranged by size:
2366 2042 1408 1365 823 802 648 639
359 159 103
MspA1I CmG'CkG
Cuts at: 1606 2241 2400 2755 3813 4431 4491 4524 4734
Size: 635 159 355 1058 618 60 33 210
Cuts at: 4734 5439 5938 6177 6204 6950 8130 8807 9955
Size: 705 499 239 27 746 1180 677 1148
Cuts at: 9955 10200 1606
Size: 245 2120
Fragments arranged by size:
2120 1180 1148 1058 746 705 677 635
618 499 355 245 239 210 159 60
33 27
NaeI GCC'GGC
Cuts at: 6506 7413 6506
Size: 907 9807
NcoI C'CATG_G
Cuts at: 513 1065 3208 4108 4386 4942 513
Size: 552 2143 900 278 556 6285
Fragments arranged by size:
6285 2143 900 556 552 278
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10714
NgoMIV G'CCGG_C
Cuts at: 6504 7411 6504
Size: 907 9807
NheI G'CTAG_C
Cuts at: 1052 7405 1052
Size: 6353 4361
NotI GC'GGCC_GC
Cuts at: 8161 8161
Size: 10714
NspI r_CATG'y
Cuts at: 1132 1229 2443 3190 7765 8072 9617 1132
Size: 97 1214 747 4575 307 1545 2229
Fragments arranged by size:
4575 2229 1545 1214 747 307 97
PasI CC'CwG_GG
Cuts at: 1348 2647 2965 5209 6112 6210 6218 6849 7870
Size: 1299 318 2244 903 98 8 631 1021
Cuts at: 7870 1348
Size: 4192
Fragments arranged by size:
4192 2244 1299 1021 903 631 318 98
8
PciI A'CATG_T
Cuts at: 3186 9613 3186
Size: 6427 4287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3301 5080 1108
Size: 2193 1779 6742
Fragments arranged by size:
6742 2193 1779
PfoI T'CCnGG_A
Cuts at: 2601 3826 5676 6809 2601
Size: 1225 1850 1133 6506
Fragments arranged by size:
6506 1850 1225 1133
PmeI GTTT'AAAC
Cuts at: 8018 8018
Size: 10714
PmlI CAC'GTG
Cuts at: 4795 6648 4795
Size: 1853 8861
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4939 4971 8783 8815 10296 10328 4939
Size: 32 3812 32 1481 32 5325
Fragments arranged by size:
5325 3812 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2151 2605 2612 2711 2969 3298 4116 4172 4484
Size: 454 7 99 258 329 818 56 312
Cuts at: 4484 5181 5369 5872 6070 6214 6813 2151
Size: 697 188 503 198 144 599 6052
Fragments arranged by size:
6052 818 697 599 503 454 329 312
258 198 188 144 99 56 7
PshAI GACnn'nnGTC
Cuts at: 1613 2367 3189 3309 3795 5031 7959 1613
Size: 754 822 120 486 1236 2928 4368
Fragments arranged by size:
4368 2928 1236 822 754 486 120
PsiI TTA'TAA
Cuts at: 8284 8284
Size: 10714
PspOMI G'GGCC_C
Cuts at: 3281 4286 4399 4592 5265 5464 5504 5885 6036
Size: 1005 113 193 673 199 40 381 151
Cuts at: 6036 7517 3281
Size: 1481 6478
Fragments arranged by size:
6478 1481 1005 673 381 199 193 151
113 40
PstI C_TGCA'G
Cuts at: 830 2984 5068 5258 5579 5830 5858 6256 7084
Size: 2154 2084 190 321 251 28 398 828
Cuts at: 7084 7638 7759 8066 830
Size: 554 121 307 3478
Fragments arranged by size:
3478 2154 2084 828 554 398 321 307
251 190 121 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10714
PvuII CAG'CTG
Cuts at: 2241 2755 3813 4431 4524 5439 5938 2241
Size: 514 1058 618 93 915 499 7017
Fragments arranged by size:
7017 1058 915 618 514 499 93
SacI G_AGCT'C
Cuts at: 6445 6445
Size: 10714
SalI G'TCGA_C
Cuts at: 1936 4727 6423 8056 1936
Size: 2791 1696 1633 4594
Fragments arranged by size:
4594 2791 1696 1633
SanDI GG'GwC_CC
Cuts at: 2969 4484 5872 6070 6214 2969
Size: 1515 1388 198 144 7469
Fragments arranged by size:
7469 1515 1388 198 144
SapI GCTCTTCn'nnn_
Cuts at: 6402 6402
Size: 10714
SbfI CC_TGCA'GG
Cuts at: 8066 8066
Size: 10714
ScaI AGT'ACT
Cuts at: 5777 8962 10674 5777
Size: 3185 1712 5817
Fragments arranged by size:
5817 3185 1712
SexAI A'CCwGG_T
Cuts at: 7149 7149
Size: 10714
SfcI C'TryA_G
Cuts at: 826 1045 2980 4158 4180 4447 5064 5254 5575
Size: 219 1935 1178 22 267 617 190 321
Cuts at: 5575 5826 5854 6252 7080 7634 7755 8062 9197
Size: 251 28 398 828 554 121 307 1135
Cuts at: 9197 9878 10069 826
Size: 681 191 1471
Fragments arranged by size:
1935 1471 1178 1135 828 681 617 554
398 321 307 267 251 219 191 190
121 28 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10714
SgrAI Cr'CCGG_yG
Cuts at: 1700 7411 1700
Size: 5711 5003
SmaI CCC'GGG
Cuts at: 1872 3102 5586 5954 6041 8041 1872
Size: 1230 2484 368 87 2000 4545
Fragments arranged by size:
4545 2484 2000 1230 368 87
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6536 6965 8818 9719 9981 820
Size: 197 926 4593 429 1853 901 262 1553
Fragments arranged by size:
4593 1853 1553 926 901 429 262 197
SnaBI TAC'GTA
Cuts at: 493 8505 493
Size: 8012 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10714
SphI G_CATG'C
Cuts at: 1132 2443 7765 8072 1132
Size: 1311 5322 307 3774
Fragments arranged by size:
5322 3774 1311 307
SrfI GCCC'GGGC
Cuts at: 6041 6041
Size: 10714
SspI AAT'ATT
Cuts at: 5 52 8638 5
Size: 47 8586 2081
Fragments arranged by size:
8586 2081 47
StuI AGG'CCT
Cuts at: 1517 2414 4360 4719 4816 5304 5860 1517
Size: 897 1946 359 97 488 556 6371
Fragments arranged by size:
6371 1946 897 556 488 359 97
StyI C'CwwG_G
Cuts at: 513 1065 2266 2723 3208 4108 4386 4681 4771
Size: 552 1201 457 485 900 278 295 90
Cuts at: 4771 4942 5232 5529 6388 6820 7993 8153 513
Size: 171 290 297 859 432 1173 160 3074
Fragments arranged by size:
3074 1201 1173 900 859 552 485 457
432 297 295 290 278 171 160 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 2314 3474 5709 6140 6149 7852
Size: 1255 588 146 1160 2235 431 9 1703
Cuts at: 7852 8741 8758 8911 9096 9541 325
Size: 889 17 153 185 445 1498
Fragments arranged by size:
2235 1703 1498 1255 1160 889 588 445
431 185 153 146 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4952 5775 6802
Size: 274 80 33 51 1443 2975 823 1027
Cuts at: 6802 8960 10672 96
Size: 2158 1712 138
Fragments arranged by size:
2975 2158 1712 1443 1027 823 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 4255 4287 5672 5704 5864 5896 5986
Size: 32 1050 32 1385 32 160 32 90
Cuts at: 5986 6018 6182 6214 6597 6629 10674 10706 3173
Size: 32 164 32 383 32 4045 32 3181
Fragments arranged by size:
4045 3181 1385 1050 383 164 160 90
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6484 7540 7594 7648 1316
Size: 5168 1056 54 54 4382
Fragments arranged by size:
5168 4382 1056 54 54
XbaI T'CTAG_A
Cuts at: 8050 8050
Size: 10714
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2861 3008 3206 3710 4760 5108 7138 2165
Size: 696 147 198 504 1050 348 2030 5741
Fragments arranged by size:
5741 2030 1050 696 504 348 198 147
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10714
XmaI C'CCGG_G
Cuts at: 1870 3100 5584 5952 6039 8039 1870
Size: 1230 2484 368 87 2000 4545
Fragments arranged by size:
4545 2484 2000 1230 368 87
XmnI GAAnn'nnTTC
Cuts at: 8843 8843
Size: 10714
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4693 7420 8522 276
Size: 53 83 186 936 3159 2727 1102 2468
Fragments arranged by size:
3159 2727 2468 1102 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BbeI BfrBI BsiWI BstZ17I FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacII SfiI SfoI SwaI