Restriction map for
pFN21AB9148
(Circular) (Six-base) MAPSORT of: pFN21AB9148.seq Check: 5622 from: 1 to: 6349
pFN21AB9148 6349 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3641 3641
Size: 6349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2674 2760 3692 1937
Size: 737 86 932 4594
Fragments arranged by size:
4594 932 737 86
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4412 5796 6064 1632
Size: 2780 1384 268 1917
Fragments arranged by size:
2780 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 2585 5248 6225 1203
Size: 1382 2663 977 1327
Fragments arranged by size:
2663 1382 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 2163 5078 2163
Size: 2915 3434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6349
AlwNI CAG_nnn'CTG
Cuts at: 3080 4092 5664 3080
Size: 1012 1572 3765
Fragments arranged by size:
3765 1572 1012
ApaLI G'TGCA_C
Cuts at: 2417 4406 5562 2417
Size: 1989 1156 3204
Fragments arranged by size:
3204 1989 1156
ApoI r'AATT_y
Cuts at: 1493 3455 3658 3884 4036 6145 1493
Size: 1962 203 226 152 2109 1697
Fragments arranged by size:
2109 1962 1697 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2130 3674 1870
Size: 73 187 1544 4545
Fragments arranged by size:
4545 1544 187 73
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3670 5125 618
Size: 325 217 627 1883 1455 1842
Fragments arranged by size:
1883 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3060 3668 1391
Size: 1669 608 4072
Fragments arranged by size:
4072 1669 608
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2754 928
Size: 971 855 4523
Fragments arranged by size:
4523 971 855
BbvCI CC'TCA_GC
Cuts at: 3126 3126
Size: 6349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2029 4241 5451 2029
Size: 2212 1210 2927
Fragments arranged by size:
2927 2212 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4960 136
Size: 107 122 71 631 605 3288 1525
Fragments arranged by size:
3288 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2365 3261 6344 2365
Size: 896 3083 2370
Fragments arranged by size:
3083 2370 896
BlpI GC'TnA_GC
Cuts at: 2171 2176 3766 2171
Size: 5 1590 4754
Fragments arranged by size:
4754 1590 5
Bme1580I G_kGCm'C
Cuts at: 1790 2321 2421 4410 5566 1790
Size: 531 100 1989 1156 2573
Fragments arranged by size:
2573 1989 1156 531 100
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4080 5038 442
Size: 416 53 83 399 24 2663 958 1753
Fragments arranged by size:
2663 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2912 3356 5009 1239
Size: 1673 444 1653 2579
Fragments arranged by size:
2579 1673 1653 444
Bpu10I CC'TnA_GC
Cuts at: 3126 3126
Size: 6349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2997 4474 5339 5637 2997
Size: 1477 865 298 3709
Fragments arranged by size:
3709 1477 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2150 5012 882
Size: 617 651 2862 2219
Fragments arranged by size:
2862 2219 651 617
BsaAI yAC'GTr
Cuts at: 493 1204 2851 4140 493
Size: 711 1647 1289 2702
Fragments arranged by size:
2702 1647 1289 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4156 4538 5173 275
Size: 53 83 186 936 2623 382 635 1451
Fragments arranged by size:
2623 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3282 4782 5180 5454 5601 6012 3282
Size: 1500 398 274 147 411 3619
Fragments arranged by size:
3619 1500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3539 3569 1178
Size: 30 165 30 2136 30 3958
Fragments arranged by size:
3958 2136 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2971 3557 3560 1186
Size: 1785 586 3 3975
Fragments arranged by size:
3975 1785 586 3
BseYI C'CCAG_C
Cuts at: 5552 6048 5552
Size: 496 5853
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2831 1163
Size: 573 1095 4681
Fragments arranged by size:
4681 1095 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4560 5588 1540
Size: 275 186 1798 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2421 3206 3609 3624 4410 4495 5566 2421
Size: 785 403 15 786 85 1071 3204
Fragments arranged by size:
3204 1071 786 785 403 85 15
BsmI GAATG_Cn'
Cuts at: 3195 3860 3953 3195
Size: 665 93 5591
Fragments arranged by size:
5591 665 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 2421 3060 3206 3609 3624 3668
Size: 399 531 100 639 146 403 15 44
Cuts at: 3668 4410 4495 5566 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 639 531 403 399 146
100 85 44 15
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 2590 2683 4238 5968 2590
Size: 93 1555 1730 2971
Fragments arranged by size:
2971 1730 1555 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3641 3704 844
Size: 2797 63 3489
Fragments arranged by size:
3489 2797 63
BsrBI CCG'CTC
Cuts at: 1394 1823 4236 1394
Size: 429 2413 3507
Fragments arranged by size:
3507 2413 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2559 4844 5018 65
Size: 1183 1311 2285 174 1396
Fragments arranged by size:
2285 1396 1311 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4993 5180 1700
Size: 110 3183 187 2869
Fragments arranged by size:
3183 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2403 96
Size: 2307 4042
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2622 794
Size: 1828 4521
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 2365 3261 3679 4046 4431 4448 5900 5986
Size: 393 896 418 367 385 17 1452 86
Cuts at: 5986 5998 6344 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 896 418 393 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2675 2675
Size: 6349
BtgI C'CryG_G
Cuts at: 513 1065 2004 2137 513
Size: 552 939 133 4725
Fragments arranged by size:
4725 939 552 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 3530 3559 3865 4659 4679 3530
Size: 29 306 794 20 5200
Fragments arranged by size:
5200 794 306 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2756 5356 809
Size: 507 1440 2600 1802
Fragments arranged by size:
2600 1802 1440 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2723 2776 3796
Size: 54 1425 50 275 18 893 53 1020
Cuts at: 3796 4685 6314 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1020 893 889 275 54 53
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 2955 3184 4279 1353
Size: 1125 477 229 1095 3423
Fragments arranged by size:
3423 1125 1095 477 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 4936 5310 5456 387
Size: 1064 138 459 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 459 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2912 3356 4412 5009 5796 6064 1239
Size: 393 1280 444 1056 597 787 268 1524
Fragments arranged by size:
1524 1280 1056 787 597 444 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2793 2793
Size: 6349
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
EcoRV GAT'ATC
Cuts at: 2824 2824
Size: 6349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2210 2641 3195 2210
Size: 431 554 5364
Fragments arranged by size:
5364 554 431
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 5496 1678
Size: 3818 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 2322 2354 2954
Size: 32 135 32 577 32 141 32 600
Cuts at: 2954 2986 4990 5022 5064 5096 1373
Size: 32 2004 32 42 32 2626
Fragments arranged by size:
2626 2004 600 577 141 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2761 2833 3153 3693 3939 669
Size: 988 281 823 72 320 540 246 3079
Fragments arranged by size:
3079 988 823 540 320 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 2504 3948 2504
Size: 1444 4905
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2849 3273 5463 5647 1305
Size: 1544 424 2190 184 2007
Fragments arranged by size:
2190 2007 1544 424 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2725 2778 10
Size: 54 1425 343 893 53 3581
Fragments arranged by size:
3581 1425 893 343 54 53
MslI CAynn'nnrTG
Cuts at: 518 1166 4307 4666 4825 518
Size: 648 3141 359 159 2042
Fragments arranged by size:
3141 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2426 3765 4442 5590 5835 1606
Size: 564 256 1339 677 1148 245 2120
Fragments arranged by size:
2120 1339 1148 677 564 256 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4858
Fragments arranged by size:
4858 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 2589 3241 3403 3707 5252 1132
Size: 97 1360 652 162 304 1545 2229
Fragments arranged by size:
2229 1545 1360 652 304 162 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 2585 5248 2585
Size: 2663 3686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PmlI CAC'GTG
Cuts at: 2851 2851
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2586 1613
Size: 973 5376
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 6317
PstI C_TGCA'G
Cuts at: 830 2610 3701 830
Size: 1780 1091 3478
Fragments arranged by size:
3478 1780 1091
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2170 2426 2170
Size: 256 6093
SalI G'TCGA_C
Cuts at: 1936 2759 3691 1936
Size: 823 932 4594
Fragments arranged by size:
4594 932 823
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 2361 2606 3390 3697 4832 5513 5704
Size: 219 1316 245 784 307 1135 681 191
Cuts at: 5704 826
Size: 1471
Fragments arranged by size:
1471 1316 1135 784 681 307 245 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2130 3012 4453 5354 5616 820
Size: 197 926 187 882 1441 901 262 1553
Fragments arranged by size:
1553 1441 926 901 882 262 197 187
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 2879 1517
Size: 1362 4987
StyI C'CwwG_G
Cuts at: 513 1065 2004 3788 513
Size: 552 939 1784 3074
Fragments arranged by size:
3074 1784 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2964 3221 4376 4393 4546 4731 5176
Size: 1255 1384 257 1155 17 153 185 445
Cuts at: 5176 325
Size: 1498
Fragments arranged by size:
1498 1384 1255 1155 445 257 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2403 4595 6307
Size: 274 80 33 51 1443 426 2192 1712
Cuts at: 6307 96
Size: 138
Fragments arranged by size:
2192 1712 1443 426 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6309 6341 6309
Size: 32 6317
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 2130 1943
Size: 187 6162
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 2814 4478 2814
Size: 1664 4685
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI