Restriction map for
pFN21AB9131
(Circular) (Six-base) MAPSORT of: pFN21AB9131.seq Check: 54 from: 1 to: 6442
pFN21AB9131 6442 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 1937
Size: 1848 4594
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 4505 5889 6157 1632
Size: 815 2058 1384 268 1917
Fragments arranged by size:
2058 1917 1384 815 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 5341 6318 1203
Size: 4138 977 1327
Fragments arranged by size:
4138 1327 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 2268 5171 2268
Size: 2903 3539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6442
AlwNI CAG_nnn'CTG
Cuts at: 4185 5757 4185
Size: 1572 4870
ApaLI G'TGCA_C
Cuts at: 2250 3606 4499 5655 2250
Size: 1356 893 1156 3037
Fragments arranged by size:
3037 1356 1156 893
ApoI r'AATT_y
Cuts at: 1493 2288 3215 3257 3467 3751 3977 4129 6238
Size: 795 927 42 210 284 226 152 2109
Cuts at: 6238 1493
Size: 1697
Fragments arranged by size:
2109 1697 927 795 284 226 210 152
42
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 1870
Size: 73 1824 4545
Fragments arranged by size:
4545 1824 73
BamHI G'GATC_C
Cuts at: 3181 3772 4139 3181
Size: 591 367 5484
Fragments arranged by size:
5484 591 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3763 5218 618
Size: 325 217 627 1976 1455 1842
Fragments arranged by size:
1976 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2785 3720 3761 1391
Size: 1394 935 41 4072
Fragments arranged by size:
4072 1394 935 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2597 928
Size: 971 698 4773
Fragments arranged by size:
4773 971 698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4633 4667 1903
Size: 34 2696 34 3678
Fragments arranged by size:
3678 2696 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2785 4334 5544 2785
Size: 1549 1210 3683
Fragments arranged by size:
3683 1549 1210
BclI T'GATC_A
Cuts at: 1548 2899 1548
Size: 1351 5091
BfrBI ATG'CAT
Cuts at: 2989 3145 2989
Size: 156 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5053 136
Size: 107 122 71 631 605 3381 1525
Fragments arranged by size:
3381 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3286 3682 6437 3286
Size: 396 2755 3291
Fragments arranged by size:
3291 2755 396
BlpI GC'TnA_GC
Cuts at: 2053 2918 3859 2053
Size: 865 941 4636
Fragments arranged by size:
4636 941 865
Bme1580I G_kGCm'C
Cuts at: 1790 2254 3610 4503 5659 1790
Size: 464 1356 893 1156 2573
Fragments arranged by size:
2573 1356 1156 893 464
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4173 5131 442
Size: 416 53 83 399 24 2756 958 1753
Fragments arranged by size:
2756 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3186 3249 3186
Size: 63 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2723 3153 3219 5102 1239
Size: 1484 430 66 1883 2579
Fragments arranged by size:
2579 1883 1484 430 66
Bpu10I CC'TnA_GC
Cuts at: 2544 2544
Size: 6442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2044 4567 5432 5730 2044
Size: 2523 865 298 2756
Fragments arranged by size:
2756 2523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5105 882
Size: 617 3606 2219
Fragments arranged by size:
3606 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2333 4249 4631 5266
Size: 53 83 186 936 800 1916 382 635
Cuts at: 5266 275
Size: 1451
Fragments arranged by size:
1916 1451 936 800 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4875 5273 5547 5694 6105 4875
Size: 398 274 147 411 5212
Fragments arranged by size:
5212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6217
Fragments arranged by size:
6217 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2104 2721 2868 3728 1186
Size: 915 3 617 147 860 3900
Fragments arranged by size:
3900 915 860 617 147 3
BseYI C'CCAG_C
Cuts at: 3323 5645 6141 3323
Size: 2322 496 3624
Fragments arranged by size:
3624 2322 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5869
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3892 4653 5681 1540
Size: 275 186 1891 761 1028 2301
Fragments arranged by size:
2301 1891 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2254 2785 3610 3720 4503 4588 5659 2254
Size: 531 825 110 783 85 1071 3037
Fragments arranged by size:
3037 1071 825 783 531 110 85
BsmI GAATG_Cn'
Cuts at: 2020 3147 3953 4046 2020
Size: 1127 806 93 4416
Fragments arranged by size:
4416 1127 806 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2254 2785 3610 3720 3761 4503 4588
Size: 399 464 531 825 110 41 742 85
Cuts at: 4588 5659 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 825 742 531 464 399 110
85 41
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 2650 2896 4331 6061 2650
Size: 246 1435 1730 3031
Fragments arranged by size:
3031 1730 1435 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3797 844
Size: 2953 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4329 1394
Size: 429 2506 3507
Fragments arranged by size:
3507 2506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2483 2592 2902 2917 4937 5111 65
Size: 1183 1235 109 310 15 2020 174 1396
Fragments arranged by size:
2020 1396 1235 1183 310 174 109 15
BsrFI r'CCGG_y
Cuts at: 1700 1810 5086 5273 1700
Size: 110 3276 187 2869
Fragments arranged by size:
3276 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6442
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 2773 1184
Size: 1589 4853
BstXI CCAn_nnnn'nTGG
Cuts at: 2615 2875 2615
Size: 260 6182
BstYI r'GATC_y
Cuts at: 1972 3181 3286 3445 3682 3772 4139 4524 4541
Size: 1209 105 159 237 90 367 385 17
Cuts at: 4541 5993 6079 6091 6437 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1209 385 367 346 237 159
105 90 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4951
Fragments arranged by size:
4951 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 3246 3958 4752 4772 3246
Size: 712 794 20 4916
Fragments arranged by size:
4916 794 712 20
ClaI AT'CG_AT
Cuts at: 1554 3550 1554
Size: 1996 4446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 2892 3318 3696 3730 3746 2892
Size: 426 378 34 16 5588
Fragments arranged by size:
5588 426 378 34 16
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5449 809
Size: 507 4133 1802
Fragments arranged by size:
4133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3889 4778 6407
Size: 54 1425 50 275 18 2059 889 1629
Cuts at: 6407 8
Size: 43
Fragments arranged by size:
2059 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2122 2731 3501 4372 1353
Size: 769 609 770 871 3423
Fragments arranged by size:
3423 871 770 769 609
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5029 5403 5549 387
Size: 1064 138 3440 374 146 1280
Fragments arranged by size:
3440 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2783 3718 2783
Size: 935 5507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2723 3153 3219 4505 5102 5889
Size: 393 815 276 430 66 1286 597 787
Cuts at: 5889 6157 1239
Size: 268 1524
Fragments arranged by size:
1524 1286 815 787 597 430 393 276
268 66
EcoNI CCTnn'n_nnAGG
Cuts at: 3317 3317
Size: 6442
EcoO109I rG'GnC_Cy
Cuts at: 3615 3615
Size: 6442
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2812 2398
Size: 414 6028
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 5589 1678
Size: 3911 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2254 2286 2318 2350 2767
Size: 32 135 32 682 32 32 32 417
Cuts at: 2767 2799 3189 3221 3413 3445 3674 3706 5083
Size: 32 390 32 192 32 229 32 1377
Cuts at: 5083 5115 5157 5189 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1377 682 417 390 229 192 135
42 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3310 3571 3786 4032 669
Size: 988 281 1372 261 215 246 3079
Fragments arranged by size:
3079 1372 988 281 261 246 215
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2485 3517 5556 5740 1305
Size: 1180 1032 2039 184 2007
Fragments arranged by size:
2039 2007 1180 1032 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4620
Fragments arranged by size:
4620 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2277 2958 3639 4400 4759 4918
Size: 648 843 268 681 681 761 359 159
Cuts at: 4918 518
Size: 2042
Fragments arranged by size:
2042 843 761 681 681 648 359 268
159
MspA1I CmG'CkG
Cuts at: 1606 2683 2831 3527 3858 4535 5683 5928 1606
Size: 1077 148 696 331 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 696 677 331 245 148
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4951
Fragments arranged by size:
4951 939 552
NdeI CA'TA_TG
Cuts at: 387 3175 387
Size: 2788 3654
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NsiI A_TGCA'T
Cuts at: 2991 3147 2991
Size: 156 6286
NspI r_CATG'y
Cuts at: 1132 1229 2842 3706 3800 5345 1132
Size: 97 1613 864 94 1545 2229
Fragments arranged by size:
2229 1613 1545 864 97 94
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6442
PciI A'CATG_T
Cuts at: 5341 5341
Size: 6442
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2508 2540 4511 4543 6024 6056 2508
Size: 32 1971 32 1481 32 2894
Fragments arranged by size:
2894 1971 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3615 3615
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PstI C_TGCA'G
Cuts at: 830 3547 3794 830
Size: 2717 247 3478
Fragments arranged by size:
3478 2717 247
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
PvuII CAG'CTG
Cuts at: 2683 2831 3527 2683
Size: 148 696 5598
Fragments arranged by size:
5598 696 148
SacI G_AGCT'C
Cuts at: 2785 3720 2785
Size: 935 5507
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SapI GCTCTTCn'nnn_
Cuts at: 2731 2731
Size: 6442
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SexAI A'CCwGG_T
Cuts at: 3303 3303
Size: 6442
SfcI C'TryA_G
Cuts at: 826 1045 2935 3543 3790 4925 5606 5797 826
Size: 219 1890 608 247 1135 681 191 1471
Fragments arranged by size:
1890 1471 1135 681 608 247 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2059 4546 5447 5709 820
Size: 197 926 116 2487 901 262 1553
Fragments arranged by size:
2487 1553 926 901 262 197 116
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 2842 3800 1132
Size: 1710 958 3774
Fragments arranged by size:
3774 1710 958
SspI AAT'ATT
Cuts at: 5 52 2191 4366 5
Size: 47 2139 2175 2081
Fragments arranged by size:
2175 2139 2081 47
StuI AGG'CCT
Cuts at: 1517 3647 1517
Size: 2130 4312
StyI C'CwwG_G
Cuts at: 513 1065 2004 3881 513
Size: 552 939 1877 3074
Fragments arranged by size:
3074 1877 939 552
SwaI ATTT'AAAT
Cuts at: 2892 2892
Size: 6442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4469 4486 4639 4824 5269 325
Size: 1255 2889 17 153 185 445 1498
Fragments arranged by size:
2889 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2412 4688 6400
Size: 274 80 33 51 1443 435 2276 1712
Cuts at: 6400 96
Size: 138
Fragments arranged by size:
2276 1712 1443 435 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6402 6434 6402
Size: 32 6410
Tth111I GACn'n_nGTC
Cuts at: 1316 3305 1316
Size: 1989 4453
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2698 2698
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 2099 2862 4571 2099
Size: 763 1709 3970
Fragments arranged by size:
3970 1709 763
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI