Restriction map for
pFN21AB9127
(Circular) (Six-base) MAPSORT of: pFN21AB9127.seq Check: 898 from: 1 to: 7162
pFN21AB9127 7162 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4972 278
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4505 1937
Size: 2568 4594
Acc65I G'GTAC_C
Cuts at: 4483 4483
Size: 7162
AclI AA'CG_TT
Cuts at: 1563 3869 4205 5289 5662 1563
Size: 2306 336 1084 373 3063
Fragments arranged by size:
3063 2306 1084 373 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5225 6609 6877 1632
Size: 3593 1384 268 1917
Fragments arranged by size:
3593 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6965
AflIII A'CryG_T
Cuts at: 1203 6061 7038 1203
Size: 4858 977 1327
Fragments arranged by size:
4858 1327 977
AgeI A'CCGG_T
Cuts at: 5993 5993
Size: 7162
AhdI GACnn_n'nnGTC
Cuts at: 5891 5891
Size: 7162
AleI CACnn'nnGTG
Cuts at: 1166 2907 2967 3075 3807 4227 1166
Size: 1741 60 108 732 420 4101
Fragments arranged by size:
4101 1741 732 420 108 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7162
AlwNI CAG_nnn'CTG
Cuts at: 3102 4905 6477 3102
Size: 1803 1572 3787
Fragments arranged by size:
3787 1803 1572
ApaLI G'TGCA_C
Cuts at: 3810 4062 4230 5219 6375 3810
Size: 252 168 989 1156 4597
Fragments arranged by size:
4597 1156 989 252 168
ApoI r'AATT_y
Cuts at: 1493 2011 2269 2324 2391 2420 2638 2664 2814
Size: 518 258 55 67 29 218 26 150
Cuts at: 2814 2898 3066 3150 3234 3318 3402 3570 3654
Size: 84 168 84 84 84 84 168 84
Cuts at: 3654 3738 3822 3906 4158 4242 4300 4326 4471
Size: 84 84 84 252 84 58 26 145
Cuts at: 4471 4697 4849 6958 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 518 258 252 226 218 168
168 152 150 145 84 84 84 84
84 84 84 84 84 84 67 58
55 29 26 26
AseI AT'TA_AT
Cuts at: 160 1032 2184 5716 160
Size: 872 1152 3532 1606
Fragments arranged by size:
3532 1606 1152 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
AvaI C'yCGr_G
Cuts at: 1870 1943 4487 1870
Size: 73 2544 4545
Fragments arranged by size:
4545 2544 73
BamHI G'GATC_C
Cuts at: 4492 4859 4492
Size: 367 6795
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4483 5938 618
Size: 325 217 627 2696 1455 1842
Fragments arranged by size:
2696 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2285 4481 1391
Size: 894 2196 4072
Fragments arranged by size:
4072 2196 894
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2792 3551 3956 928
Size: 971 893 759 405 4134
Fragments arranged by size:
4134 971 893 759 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5353 5387 1903
Size: 34 3416 34 3678
Fragments arranged by size:
3678 3416 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5054 6264 5054
Size: 1210 5952
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7162
BfrBI ATG'CAT
Cuts at: 4417 4417
Size: 7162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3893 5773 136
Size: 107 122 71 631 605 2221 1880 1525
Fragments arranged by size:
2221 1880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2708 3278 7157 2708
Size: 570 3879 2713
Fragments arranged by size:
3879 2713 570
BlpI GC'TnA_GC
Cuts at: 2284 4579 2284
Size: 2295 4867
Bme1580I G_kGCm'C
Cuts at: 1790 3814 3898 4066 4234 5223 6379 1790
Size: 2024 84 168 168 989 1156 2573
Fragments arranged by size:
2573 2024 1156 989 168 168 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7162
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3840 4893 5851
Size: 416 53 83 399 24 2423 1053 958
Cuts at: 5851 442
Size: 1753
Fragments arranged by size:
2423 1753 1053 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 5822 1239
Size: 1098 3485 2579
Fragments arranged by size:
3485 2579 1098
Bpu10I CC'TnA_GC
Cuts at: 3997 3997
Size: 7162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3203 5287 6152 6450 3203
Size: 2084 865 298 3915
Fragments arranged by size:
3915 2084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5825 882
Size: 617 4326 2219
Fragments arranged by size:
4326 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4953 493
Size: 711 3749 2702
Fragments arranged by size:
3749 2702 711
BsaBI GATnn'nnATC
Cuts at: 7156 7156
Size: 7162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4969 5351 5986 275
Size: 53 83 186 936 3436 382 635 1451
Fragments arranged by size:
3436 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5595 5993 6267 6414 6825 5595
Size: 398 274 147 411 5932
Fragments arranged by size:
5932 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6937
Fragments arranged by size:
6937 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7162
BseYI C'CCAG_C
Cuts at: 6365 6861 6365
Size: 496 6666
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6589
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4612 5373 6401 1540
Size: 275 186 2611 761 1028 2301
Fragments arranged by size:
2611 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2285 2890 3058 3142 3310 3478 3814 3982 4066
Size: 605 168 84 168 168 336 168 84
Cuts at: 4066 4234 5223 5308 6379 2285
Size: 168 989 85 1071 3068
Fragments arranged by size:
3068 1071 989 605 336 168 168 168
168 168 85 84 84
BsmI GAATG_Cn'
Cuts at: 3098 4022 4283 4419 4673 4766 3098
Size: 924 261 136 254 93 5494
Fragments arranged by size:
5494 924 261 254 136 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2890 3058 3142 3310 3478 3814
Size: 399 495 605 168 84 168 168 336
Cuts at: 3814 3898 3982 4066 4234 4481 5223 5308 6379
Size: 84 84 84 168 247 742 85 1071
Cuts at: 6379 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 605 495 399 336 247
168 168 168 168 85 84 84 84
84
BspEI T'CCGG_A
Cuts at: 6825 6825
Size: 7162
BspHI T'CATG_A
Cuts at: 5051 6781 5051
Size: 1730 5432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4517 844
Size: 3673 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5049 1394
Size: 429 3226 3507
Fragments arranged by size:
3507 3226 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5657 5831 65
Size: 1183 4409 174 1396
Fragments arranged by size:
4409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5806 5993 1700
Size: 110 3996 187 2869
Fragments arranged by size:
3996 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2345 2971 96
Size: 2249 626 4287
Fragments arranged by size:
4287 2249 626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7162
BssSI C'ACGA_G
Cuts at: 5222 6234 5222
Size: 1012 6150
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7162
BstBI TT'CG_AA
Cuts at: 5966 5966
Size: 7162
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7162
BstYI r'GATC_y
Cuts at: 1972 2708 3278 4492 4859 5244 5261 6713 6799
Size: 736 570 1214 367 385 17 1452 86
Cuts at: 6799 6811 7157 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1214 736 570 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3890 3890
Size: 7162
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6610
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6423
Fragments arranged by size:
6423 683 56
BtsI GCAGTG_nn'
Cuts at: 2779 3103 4027 4678 5472 5492 2779
Size: 324 924 651 794 20 4449
Fragments arranged by size:
4449 924 794 651 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2107 2142 2683 2718 529
Size: 35 1543 35 541 35 4973
Fragments arranged by size:
4973 1543 541 35 35 35
DraI TTT'AAA
Cuts at: 4466 4466
Size: 7162
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6169 809
Size: 507 4853 1802
Fragments arranged by size:
4853 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4609 5498 7127
Size: 54 1425 50 275 18 2779 889 1629
Cuts at: 7127 8
Size: 43
Fragments arranged by size:
2779 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4609 1537
Size: 275 2797 4090
Fragments arranged by size:
4090 2797 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5092 1353
Size: 3739 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5749 6123 6269 387
Size: 1064 138 4160 374 146 1280
Fragments arranged by size:
4160 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2283 2283
Size: 7162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 5225 5822 6609 6877 1239
Size: 393 705 2888 597 787 268 1524
Fragments arranged by size:
2888 1524 787 705 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2496 2496
Size: 7162
EcoRI G'AATT_C
Cuts at: 2391 2638 2664 2814 2898 3066 3150 3234 3318
Size: 247 26 150 84 168 84 84 84
Cuts at: 3318 3402 3570 3654 3738 3822 3906 4158 4242
Size: 84 168 84 84 84 84 252 84
Cuts at: 4242 4326 4471 4849 2391
Size: 84 145 378 4704
Fragments arranged by size:
4704 378 252 247 168 168 150 145
84 84 84 84 84 84 84 84
84 84 84 26
FspI TGC'GCA
Cuts at: 5668 5668
Size: 7162
HaeII r_GCGC'y
Cuts at: 1678 6309 1678
Size: 4631 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2779 2811 2863 2895 3043
Size: 32 135 32 1207 32 52 32 148
Cuts at: 3043 3075 3295 3327 3955 3987 5803 5835 5877
Size: 32 220 32 628 32 1816 32 42
Cuts at: 5877 5909 1373
Size: 32 2626
Fragments arranged by size:
2626 1816 1207 628 220 148 135 52
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4506 4752 669
Size: 988 281 2568 246 3079
Fragments arranged by size:
3079 2568 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2625 4287 748
Size: 1877 1662 3623
Fragments arranged by size:
3623 1877 1662
HpaI GTT'AAC
Cuts at: 4752 4752
Size: 7162
KpnI G_GTAC'C
Cuts at: 4487 4487
Size: 7162
MfeI C'AATT_G
Cuts at: 4761 4761
Size: 7162
MluI A'CGCG_T
Cuts at: 7038 7038
Size: 7162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6276 6460 1305
Size: 4971 184 2007
Fragments arranged by size:
4971 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5340
Fragments arranged by size:
5340 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2907 2967 3075 3807 4227 4412
Size: 648 984 757 60 108 732 420 185
Cuts at: 4412 5120 5479 5638 518
Size: 708 359 159 2042
Fragments arranged by size:
2042 984 757 732 708 648 420 359
185 159 108 60
MspA1I CmG'CkG
Cuts at: 1606 2123 4578 5255 6403 6648 1606
Size: 517 2455 677 1148 245 2120
Fragments arranged by size:
2455 2120 1148 677 517 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6610
NdeI CA'TA_TG
Cuts at: 387 4054 387
Size: 3667 3495
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7162
NotI GC'GGCC_GC
Cuts at: 4609 4609
Size: 7162
NsiI A_TGCA'T
Cuts at: 4419 4419
Size: 7162
NspI r_CATG'y
Cuts at: 1132 1229 4443 4520 6065 1132
Size: 97 3214 77 1545 2229
Fragments arranged by size:
3214 2229 1545 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7162
PciI A'CATG_T
Cuts at: 6061 6061
Size: 7162
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7162
PfoI T'CCnGG_A
Cuts at: 2575 2575
Size: 7162
PmeI GTTT'AAAC
Cuts at: 4466 4466
Size: 7162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5231 5263 6744 6776 5231
Size: 32 1481 32 5617
Fragments arranged by size:
5617 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7162
PsiI TTA'TAA
Cuts at: 4732 4732
Size: 7162
PstI C_TGCA'G
Cuts at: 830 4514 830
Size: 3684 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7162
SacI G_AGCT'C
Cuts at: 2285 2285
Size: 7162
SalI G'TCGA_C
Cuts at: 1936 4504 1936
Size: 2568 4594
SbfI CC_TGCA'GG
Cuts at: 4514 4514
Size: 7162
ScaI AGT'ACT
Cuts at: 5410 7122 5410
Size: 1712 5450
SfcI C'TryA_G
Cuts at: 826 1045 4510 5645 6326 6517 826
Size: 219 3465 1135 681 191 1471
Fragments arranged by size:
3465 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7162
SmaI CCC'GGG
Cuts at: 1872 4489 1872
Size: 2617 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3218 5266 6167 6429 820
Size: 197 926 1275 2048 901 262 1553
Fragments arranged by size:
2048 1553 1275 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4953 493
Size: 4460 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7162
SphI G_CATG'C
Cuts at: 1132 4520 1132
Size: 3388 3774
SspI AAT'ATT
Cuts at: 5 52 5086 5
Size: 47 5034 2081
Fragments arranged by size:
5034 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7162
StyI C'CwwG_G
Cuts at: 513 1065 4601 513
Size: 552 3536 3074
Fragments arranged by size:
3536 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5189 5206 5359 5544 5989 325
Size: 1255 3609 17 153 185 445 1498
Fragments arranged by size:
3609 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2296 2345 2971
Size: 274 80 33 51 1443 319 49 626
Cuts at: 2971 3557 4147 4315 5408 7120 96
Size: 586 590 168 1093 1712 138
Fragments arranged by size:
1712 1443 1093 626 590 586 319 274
168 138 80 51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2092 2124 2571 2603 7122 7154 2092
Size: 32 447 32 4519 32 2100
Fragments arranged by size:
4519 2100 447 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7162
XbaI T'CTAG_A
Cuts at: 4498 4498
Size: 7162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7162
XmaI C'CCGG_G
Cuts at: 1870 4487 1870
Size: 2617 4545
XmnI GAAnn'nnTTC
Cuts at: 2627 2861 2945 3617 3785 3953 4037 4205 5291
Size: 234 84 672 168 168 84 168 1086
Cuts at: 5291 2627
Size: 4498
Fragments arranged by size:
4498 1086 672 234 168 168 168 84
84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4970 276
Size: 53 83 186 936 3436 2468
Fragments arranged by size:
3436 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI