Restriction map for
pFN21AB9116
(Circular) (Six-base) MAPSORT of: pFN21AB9116.seq Check: 1907 from: 1 to: 7021
pFN21AB9116 7021 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4831 278
Size: 53 83 186 936 3295 2468
Fragments arranged by size:
3295 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4364 1937
Size: 2427 4594
Acc65I G'GTAC_C
Cuts at: 4342 4342
Size: 7021
AclI AA'CG_TT
Cuts at: 1563 3197 5148 5521 1563
Size: 1634 1951 373 3063
Fragments arranged by size:
3063 1951 1634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4125 4161 5084 6468 6736 1632
Size: 2493 36 923 1384 268 1917
Fragments arranged by size:
2493 1917 1384 923 268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6824
AflIII A'CryG_T
Cuts at: 1203 5920 6897 1203
Size: 4717 977 1327
Fragments arranged by size:
4717 1327 977
AgeI A'CCGG_T
Cuts at: 5852 5852
Size: 7021
AhdI GACnn_n'nnGTC
Cuts at: 5750 5750
Size: 7021
AleI CACnn'nnGTG
Cuts at: 1166 3943 1166
Size: 2777 4244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7021
AlwNI CAG_nnn'CTG
Cuts at: 4207 4764 6336 4207
Size: 557 1572 4892
Fragments arranged by size:
4892 1572 557
ApaLI G'TGCA_C
Cuts at: 5078 6234 5078
Size: 1156 5865
ApoI r'AATT_y
Cuts at: 1493 2677 2989 3477 4330 4556 4708 6817 1493
Size: 1184 312 488 853 226 152 2109 1697
Fragments arranged by size:
2109 1697 1184 853 488 312 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3686 5575 160
Size: 872 2654 1889 1606
Fragments arranged by size:
2654 1889 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7021
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 4346 1870
Size: 73 219 2184 4545
Fragments arranged by size:
4545 2184 219 73
AvrII C'CTAG_G
Cuts at: 2240 2240
Size: 7021
BamHI G'GATC_C
Cuts at: 2132 4351 4718 2132
Size: 2219 367 4435
Fragments arranged by size:
4435 2219 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4342 5797 618
Size: 325 217 627 2555 1455 1842
Fragments arranged by size:
2555 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3178 4340 1391
Size: 1787 1162 4072
Fragments arranged by size:
4072 1787 1162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 928
Size: 971 198 5852
Fragments arranged by size:
5852 971 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5212 5246 1903
Size: 34 3275 34 3678
Fragments arranged by size:
3678 3275 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3148 3327 3604 4913 6123 3148
Size: 179 277 1309 1210 4046
Fragments arranged by size:
4046 1309 1210 277 179
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7021
BfrBI ATG'CAT
Cuts at: 3536 3536
Size: 7021
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5632 136
Size: 107 122 71 631 605 3960 1525
Fragments arranged by size:
3960 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2604 3975 7016 2604
Size: 1371 3041 2609
Fragments arranged by size:
3041 2609 1371
BlpI GC'TnA_GC
Cuts at: 2538 4438 2538
Size: 1900 5121
Bme1580I G_kGCm'C
Cuts at: 1790 5082 6238 1790
Size: 3292 1156 2573
Fragments arranged by size:
3292 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7021
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2623 2900 4752
Size: 416 53 83 399 24 1206 277 1852
Cuts at: 4752 5710 442
Size: 958 1753
Fragments arranged by size:
1852 1753 1206 958 416 399 277 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2468 1056
Size: 1412 5609
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2531
Size: 7021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2088 3305 3520 4290 5681 1239
Size: 849 1217 215 770 1391 2579
Fragments arranged by size:
2579 1391 1217 849 770 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2158 2905 5146 6011 6309 2158
Size: 747 2241 865 298 2870
Fragments arranged by size:
2870 2241 865 747 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5684 882
Size: 617 4185 2219
Fragments arranged by size:
4185 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3466 4812 493
Size: 711 2262 1346 2702
Fragments arranged by size:
2702 2262 1346 711
BsaBI GATnn'nnATC
Cuts at: 2609 7015 2609
Size: 4406 2615
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4298 4828 5210 5845
Size: 53 83 186 936 2765 530 382 635
Cuts at: 5845 275
Size: 1451
Fragments arranged by size:
2765 1451 936 635 530 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3549 3940 5454 5852 6126 6273 6684 3549
Size: 391 1514 398 274 147 411 3886
Fragments arranged by size:
3886 1514 411 398 391 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2061 2075 2091 2417
Size: 30 165 30 642 16 14 16 326
Cuts at: 2417 2447 1178
Size: 30 5752
Fragments arranged by size:
5752 642 326 165 30 30 30 16
16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 3429 1186
Size: 1255 988 4778
Fragments arranged by size:
4778 1255 988
BseYI C'CCAG_C
Cuts at: 2276 3163 6224 6720 2276
Size: 887 3061 496 2577
Fragments arranged by size:
3061 2577 887 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2143 2643 4130 1163
Size: 573 407 500 1487 4054
Fragments arranged by size:
4054 1487 573 500 407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4471 5232 6260 1540
Size: 275 186 2470 761 1028 2301
Fragments arranged by size:
2470 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5082 5167 6238 5082
Size: 85 1071 5865
Fragments arranged by size:
5865 1071 85
BsmI GAATG_Cn'
Cuts at: 2581 3736 4532 4625 2581
Size: 1155 796 93 4977
Fragments arranged by size:
4977 1155 796 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2734 2734
Size: 7021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3178 4340 5082 5167 6238 1391
Size: 399 1388 1162 742 85 1071 2174
Fragments arranged by size:
2174 1388 1162 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6684 6684
Size: 7021
BspHI T'CATG_A
Cuts at: 4910 6640 4910
Size: 1730 5291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4376 844
Size: 3532 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4908 1394
Size: 429 3085 3507
Fragments arranged by size:
3507 3085 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5516 5690 65
Size: 1183 4268 174 1396
Fragments arranged by size:
4268 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5665 5852 1700
Size: 110 3855 187 2869
Fragments arranged by size:
3855 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3626 96
Size: 3530 3491
BssHII G'CGCG_C
Cuts at: 1925 2286 1925
Size: 361 6660
BssSI C'ACGA_G
Cuts at: 5081 6093 5081
Size: 1012 6009
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2767 794
Size: 1973 5048
BstBI TT'CG_AA
Cuts at: 3854 5825 3854
Size: 1971 5050
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7021
BstYI r'GATC_y
Cuts at: 1972 2132 2564 2604 3270 3975 4351 4718 5103
Size: 160 432 40 666 705 376 367 385
Cuts at: 5103 5120 6572 6658 6670 7016 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 705 666 432 385 376 367
346 160 86 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 3169 3169
Size: 7021
BtgI C'CryG_G
Cuts at: 513 1065 3051 513
Size: 552 1986 4483
Fragments arranged by size:
4483 1986 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6282
Fragments arranged by size:
6282 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 4537 5331 5351 2410
Size: 2127 794 20 4080
Fragments arranged by size:
4080 2127 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7021
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6986
DraI TTT'AAA
Cuts at: 3361 3738 4325 3361
Size: 377 587 6057
Fragments arranged by size:
6057 587 377
DraIII CAC_nnn'GTG
Cuts at: 3914 3914
Size: 7021
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6028 809
Size: 507 4712 1802
Fragments arranged by size:
4712 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4048 4468 5357
Size: 54 1425 50 275 18 2218 420 889
Cuts at: 5357 6986 8
Size: 1629 43
Fragments arranged by size:
2218 1629 1425 889 420 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4468 1537
Size: 275 2656 4090
Fragments arranged by size:
4090 2656 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2997 3136 4951 1353
Size: 1644 139 1815 3423
Fragments arranged by size:
3423 1815 1644 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5608 5982 6128 387
Size: 1064 138 4019 374 146 1280
Fragments arranged by size:
4019 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 3305 3520 4125 4161 4290 5084
Size: 393 456 1217 215 605 36 129 794
Cuts at: 5084 5681 6468 6736 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1217 794 787 605 597 456 393
268 215 129 36
EcoO109I rG'GnC_Cy
Cuts at: 3061 3061
Size: 7021
EcoRI G'AATT_C
Cuts at: 2677 4330 4708 2677
Size: 1653 378 4990
Fragments arranged by size:
4990 1653 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4059 4059
Size: 7021
FspI TGC'GCA
Cuts at: 5527 5527
Size: 7021
HaeII r_GCGC'y
Cuts at: 1678 6168 1678
Size: 4490 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 3602 3634 3692
Size: 32 135 32 845 32 1153 32 58
Cuts at: 3692 3724 3890 3922 3983 4003 4015 4035 5662
Size: 32 166 32 61 20 12 20 1627
Cuts at: 5662 5694 5736 5768 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1627 1153 845 166 135 61 58
42 32 32 32 32 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 4365 4611 669
Size: 988 281 1257 1170 246 3079
Fragments arranged by size:
3079 1257 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7021
HpaI GTT'AAC
Cuts at: 3195 4611 3195
Size: 1416 5605
KpnI G_GTAC'C
Cuts at: 4346 4346
Size: 7021
MfeI C'AATT_G
Cuts at: 4620 4620
Size: 7021
MluI A'CGCG_T
Cuts at: 6897 6897
Size: 7021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2110 2133 2658 2935 3395 3672 6135 6319
Size: 805 23 525 277 460 277 2463 184
Cuts at: 6319 1305
Size: 2007
Fragments arranged by size:
2463 2007 805 525 460 277 277 184
23
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4050 10
Size: 54 1425 343 2218 2981
Fragments arranged by size:
2981 2218 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 3531 3716 3943 4979 5338 5497
Size: 648 1290 1075 185 227 1036 359 159
Cuts at: 5497 518
Size: 2042
Fragments arranged by size:
2042 1290 1075 1036 648 359 227 185
159
MspA1I CmG'CkG
Cuts at: 1606 2494 3091 4207 4437 5114 6262 6507 1606
Size: 888 597 1116 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 1116 888 677 597 245 230
NcoI C'CATG_G
Cuts at: 513 1065 3051 513
Size: 552 1986 4483
Fragments arranged by size:
4483 1986 552
NdeI CA'TA_TG
Cuts at: 387 2212 387
Size: 1825 5196
NheI G'CTAG_C
Cuts at: 1052 2464 1052
Size: 1412 5609
NotI GC'GGCC_GC
Cuts at: 4468 4468
Size: 7021
NsiI A_TGCA'T
Cuts at: 3538 3538
Size: 7021
NspI r_CATG'y
Cuts at: 1132 1229 2875 3536 3540 4379 5924 1132
Size: 97 1646 661 4 839 1545 2229
Fragments arranged by size:
2229 1646 1545 839 661 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7021
PciI A'CATG_T
Cuts at: 5920 5920
Size: 7021
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4207 1108
Size: 3099 3922
PmeI GTTT'AAAC
Cuts at: 4325 4325
Size: 7021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2370 2402 5090 5122 6603 6635 2370
Size: 32 2688 32 1481 32 2756
Fragments arranged by size:
2756 2688 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7021
PsiI TTA'TAA
Cuts at: 4591 4591
Size: 7021
PstI C_TGCA'G
Cuts at: 830 2591 4373 830
Size: 1761 1782 3478
Fragments arranged by size:
3478 1782 1761
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7021
PvuII CAG'CTG
Cuts at: 2494 4207 2494
Size: 1713 5308
SalI G'TCGA_C
Cuts at: 1936 4363 1936
Size: 2427 4594
SbfI CC_TGCA'GG
Cuts at: 4373 4373
Size: 7021
ScaI AGT'ACT
Cuts at: 3743 5269 6981 3743
Size: 1526 1712 3783
Fragments arranged by size:
3783 1712 1526
SexAI A'CCwGG_T
Cuts at: 3787 3787
Size: 7021
SfcI C'TryA_G
Cuts at: 826 1045 2587 4369 5504 6185 6376 826
Size: 219 1542 1782 1135 681 191 1471
Fragments arranged by size:
1782 1542 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7021
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7021
SmaI CCC'GGG
Cuts at: 1872 4348 1872
Size: 2476 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2137 2884 5125 6026 6288 820
Size: 197 926 194 747 2241 901 262 1553
Fragments arranged by size:
2241 1553 926 901 747 262 197 194
SnaBI TAC'GTA
Cuts at: 493 3466 4812 493
Size: 2973 1346 2702
Fragments arranged by size:
2973 2702 1346
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7021
SphI G_CATG'C
Cuts at: 1132 2875 3536 4379 1132
Size: 1743 661 843 3774
Fragments arranged by size:
3774 1743 843 661
SspI AAT'ATT
Cuts at: 5 52 3796 4945 5
Size: 47 3744 1149 2081
Fragments arranged by size:
3744 2081 1149 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7021
StyI C'CwwG_G
Cuts at: 513 1065 2224 2240 3051 4460 513
Size: 552 1159 16 811 1409 3074
Fragments arranged by size:
3074 1409 1159 811 552 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3995 5048 5065 5218 5403 5848 325
Size: 1255 2415 1053 17 153 185 445 1498
Fragments arranged by size:
2415 1498 1255 1053 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 3741 5267
Size: 274 80 33 51 1443 1649 115 1526
Cuts at: 5267 6979 96
Size: 1712 138
Fragments arranged by size:
1712 1649 1526 1443 274 138 115 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6981 7013 6981
Size: 32 6989
Tth111I GACn'n_nGTC
Cuts at: 1316 2629 1316
Size: 1313 5708
XbaI T'CTAG_A
Cuts at: 4357 4357
Size: 7021
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7021
XmaI C'CCGG_G
Cuts at: 1870 4346 1870
Size: 2476 4545
XmnI GAAnn'nnTTC
Cuts at: 5150 5150
Size: 7021
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4829 276
Size: 53 83 186 936 3295 2468
Fragments arranged by size:
3295 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI