Restriction map for
pFN21AB9101
(Circular) (Six-base) MAPSORT of: pFN21AB9101.seq Check: 1235 from: 1 to: 8179
pFN21AB9101 8179 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4390 4390
Size: 8179
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5989 278
Size: 53 83 186 936 4453 2468
Fragments arranged by size:
4453 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5522 1937
Size: 3585 4594
Acc65I G'GTAC_C
Cuts at: 5500 5500
Size: 8179
AclI AA'CG_TT
Cuts at: 1563 3875 6306 6679 1563
Size: 2312 2431 373 3063
Fragments arranged by size:
3063 2431 2312 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 6242 7626 7894 1632
Size: 767 3843 1384 268 1917
Fragments arranged by size:
3843 1917 1384 767 268
AflII C'TTAA_G
Cuts at: 820 1017 4201 820
Size: 197 3184 4798
Fragments arranged by size:
4798 3184 197
AflIII A'CryG_T
Cuts at: 1203 7078 8055 1203
Size: 5875 977 1327
Fragments arranged by size:
5875 1327 977
AgeI A'CCGG_T
Cuts at: 7010 7010
Size: 8179
AhdI GACnn_n'nnGTC
Cuts at: 2122 4136 6908 2122
Size: 2014 2772 3393
Fragments arranged by size:
3393 2772 2014
AleI CACnn'nnGTG
Cuts at: 1166 4310 1166
Size: 3144 5035
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2460 2505 1184
Size: 1276 45 6858
Fragments arranged by size:
6858 1276 45
AlwNI CAG_nnn'CTG
Cuts at: 2108 3365 4085 5084 5922 7494 2108
Size: 1257 720 999 838 1572 2793
Fragments arranged by size:
2793 1572 1257 999 838 720
ApaLI G'TGCA_C
Cuts at: 2036 3858 4313 6236 7392 2036
Size: 1822 455 1923 1156 2823
Fragments arranged by size:
2823 1923 1822 1156 455
ApoI r'AATT_y
Cuts at: 1493 2323 3469 3736 3901 4296 4706 4727 4928
Size: 830 1146 267 165 395 410 21 201
Cuts at: 4928 5488 5714 5866 7975 1493
Size: 560 226 152 2109 1697
Fragments arranged by size:
2109 1697 1146 830 560 410 395 267
226 201 165 152 21
AseI AT'TA_AT
Cuts at: 160 1032 6733 160
Size: 872 5701 1606
Fragments arranged by size:
5701 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8179
AvaI C'yCGr_G
Cuts at: 1870 1943 2844 5504 1870
Size: 73 901 2660 4545
Fragments arranged by size:
4545 2660 901 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2097 2130 2097
Size: 33 8146
BamHI G'GATC_C
Cuts at: 3418 5509 5876 3418
Size: 2091 367 5721
Fragments arranged by size:
5721 2091 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5500 6955 618
Size: 325 217 627 3713 1455 1842
Fragments arranged by size:
3713 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5498 1391
Size: 4107 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2262 4407 928
Size: 971 363 2145 4700
Fragments arranged by size:
4700 2145 971 363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2825 2859 6370 6404 1903
Size: 34 888 34 3511 34 3678
Fragments arranged by size:
3678 3511 888 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6071 7281 6071
Size: 1210 6969
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8179
BfrBI ATG'CAT
Cuts at: 2083 2083
Size: 8179
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6790 136
Size: 107 122 71 631 605 5118 1525
Fragments arranged by size:
5118 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8174 8174
Size: 8179
BlpI GC'TnA_GC
Cuts at: 4753 5596 4753
Size: 843 7336
Bme1580I G_kGCm'C
Cuts at: 1790 2040 3862 4317 4342 4663 6240 7396 1790
Size: 250 1822 455 25 321 1577 1156 2573
Fragments arranged by size:
2573 1822 1577 1156 455 321 250 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8179
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3627 5106 5910
Size: 416 53 83 399 24 2210 1479 804
Cuts at: 5910 6868 442
Size: 958 1753
Fragments arranged by size:
2210 1753 1479 958 804 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2354 3021 1056
Size: 1298 667 6214
Fragments arranged by size:
6214 1298 667
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 8179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3636 4127 6839 1239
Size: 2397 491 2712 2579
Fragments arranged by size:
2712 2579 2397 491
Bpu10I CC'TnA_GC
Cuts at: 3174 5049 3174
Size: 1875 6304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 4339 6304 7169 7467 2459
Size: 1880 1965 865 298 3171
Fragments arranged by size:
3171 1965 1880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3699 3943 6842 882
Size: 617 2200 244 2899 2219
Fragments arranged by size:
2899 2219 2200 617 244
BsaAI yAC'GTr
Cuts at: 493 1204 5970 493
Size: 711 4766 2702
Fragments arranged by size:
4766 2702 711
BsaBI GATnn'nnATC
Cuts at: 2607 3264 8173 2607
Size: 657 4909 2613
Fragments arranged by size:
4909 2613 657
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5986 6368 7003 275
Size: 53 83 186 936 4453 382 635 1451
Fragments arranged by size:
4453 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6612 7010 7284 7431 7842 6612
Size: 398 274 147 411 6949
Fragments arranged by size:
6949 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7954
Fragments arranged by size:
7954 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2649 1186
Size: 1463 6716
BseYI C'CCAG_C
Cuts at: 2129 3953 4976 7382 7878 2129
Size: 1824 1023 2406 496 2430
Fragments arranged by size:
2430 2406 1824 1023 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 4388 5085 1163
Size: 573 489 2163 697 4257
Fragments arranged by size:
4257 2163 697 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5629 6390 7418 1540
Size: 275 186 3628 761 1028 2301
Fragments arranged by size:
3628 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 3662 3862 4317 5055 6240 6325 7396 2040
Size: 1622 200 455 738 1185 85 1071 2823
Fragments arranged by size:
2823 1622 1185 1071 738 455 200 85
BsmI GAATG_Cn'
Cuts at: 2290 3924 5690 5783 2290
Size: 1634 1766 93 4686
Fragments arranged by size:
4686 1766 1634 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 3662 3862 4317 4342 4663 5055
Size: 399 250 1622 200 455 25 321 392
Cuts at: 5055 5498 6240 6325 7396 1391
Size: 443 742 85 1071 2174
Fragments arranged by size:
2174 1622 1071 742 455 443 399 392
321 250 200 85 25
BspEI T'CCGG_A
Cuts at: 7842 7842
Size: 8179
BspHI T'CATG_A
Cuts at: 2319 2500 2691 6068 7798 2319
Size: 181 191 3377 1730 2700
Fragments arranged by size:
3377 2700 1730 191 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3074 4390 5534 844
Size: 2230 1316 1144 3489
Fragments arranged by size:
3489 2230 1316 1144
BsrBI CCG'CTC
Cuts at: 1394 1823 6066 1394
Size: 429 4243 3507
Fragments arranged by size:
4243 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6674 6848 65
Size: 1183 5426 174 1396
Fragments arranged by size:
5426 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6823 7010 1700
Size: 110 5013 187 2869
Fragments arranged by size:
5013 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4959 96
Size: 4863 3316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8179
BssSI C'ACGA_G
Cuts at: 6239 7251 6239
Size: 1012 7167
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8179
BstBI TT'CG_AA
Cuts at: 6983 6983
Size: 8179
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8179
BstXI CCAn_nnnn'nTGG
Cuts at: 2981 3937 2981
Size: 956 7223
BstYI r'GATC_y
Cuts at: 1972 2228 3418 5509 5876 6261 6278 7730 7816
Size: 256 1190 2091 367 385 17 1452 86
Cuts at: 7816 7828 8174 1972
Size: 12 346 1977
Fragments arranged by size:
2091 1977 1452 1190 385 367 346 256
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3561 4428 3561
Size: 867 7312
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7627
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7440
Fragments arranged by size:
7440 683 56
BtsI GCAGTG_nn'
Cuts at: 2176 3295 5695 6489 6509 2176
Size: 1119 2400 794 20 3846
Fragments arranged by size:
3846 2400 1119 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2724 2759 529
Size: 35 2160 35 5949
Fragments arranged by size:
5949 2160 35 35
DraI TTT'AAA
Cuts at: 2328 2511 2859 4408 4615 4732 4947 5483 2328
Size: 183 348 1549 207 117 215 536 5024
Fragments arranged by size:
5024 1549 536 348 215 207 183 117
DraIII CAC_nnn'GTG
Cuts at: 3914 3914
Size: 8179
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7186 809
Size: 507 5870 1802
Fragments arranged by size:
5870 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2907 2926 3520
Size: 54 1425 50 275 18 1077 19 594
Cuts at: 3520 5626 6515 8144 8
Size: 2106 889 1629 43
Fragments arranged by size:
2106 1629 1425 1077 889 594 275 54
50 43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 5626 1537
Size: 275 3814 4090
Fragments arranged by size:
4090 3814 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 2136 2294 3936 4169 4692 5316 6109
Size: 660 123 158 1642 233 523 624 793
Cuts at: 6109 1353
Size: 3423
Fragments arranged by size:
3423 1642 793 660 624 523 233 158
123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6766 7140 7286 387
Size: 1064 138 5177 374 146 1280
Fragments arranged by size:
5177 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 3636 4127 6242 6839 7626 7894
Size: 393 767 1237 491 2115 597 787 268
Cuts at: 7894 1239
Size: 1524
Fragments arranged by size:
2115 1524 1237 787 767 597 491 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 4089 4089
Size: 8179
EcoO109I rG'GnC_Cy
Cuts at: 4082 4680 5123 4082
Size: 598 443 7138
Fragments arranged by size:
7138 598 443
EcoRI G'AATT_C
Cuts at: 3469 3736 5488 5866 3469
Size: 267 1752 378 5782
Fragments arranged by size:
5782 1752 378 267
EcoRV GAT'ATC
Cuts at: 3442 3442
Size: 8179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3518 5345 5391 3518
Size: 1827 46 6306
Fragments arranged by size:
6306 1827 46
FspI TGC'GCA
Cuts at: 6685 6685
Size: 8179
HaeII r_GCGC'y
Cuts at: 1678 7326 1678
Size: 5648 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2431 2463 2478
Size: 32 135 32 536 32 291 32 15
Cuts at: 2478 2510 4122 4154 4394 4426 5270 5302 6820
Size: 32 1612 32 240 32 844 32 1518
Cuts at: 6820 6852 6894 6926 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1612 1518 844 536 291 240 135
42 32 32 32 32 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2116 2365 3969 4073 5523 5769
Size: 988 281 178 249 1604 104 1450 246
Cuts at: 5769 669
Size: 3079
Fragments arranged by size:
3079 1604 1450 988 281 249 246 178
104
HindIII A'AGCT_T
Cuts at: 748 2343 4583 4798 5349 748
Size: 1595 2240 215 551 3578
Fragments arranged by size:
3578 2240 1595 551 215
HpaI GTT'AAC
Cuts at: 3969 5769 3969
Size: 1800 6379
KpnI G_GTAC'C
Cuts at: 5504 5504
Size: 8179
MfeI C'AATT_G
Cuts at: 3509 5778 3509
Size: 2269 5910
MluI A'CGCG_T
Cuts at: 8055 8055
Size: 8179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2650 3336 7293 7477 1305
Size: 1345 686 3957 184 2007
Fragments arranged by size:
3957 2007 1345 686 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2909 2928 3522 10
Size: 54 1425 343 1077 19 594 4667
Fragments arranged by size:
4667 1425 1077 594 343 54 19
MslI CAynn'nnrTG
Cuts at: 518 1166 2979 3528 3576 3935 4310 6137 6496
Size: 648 1813 549 48 359 375 1827 359
Cuts at: 6496 6655 518
Size: 159 2042
Fragments arranged by size:
2042 1827 1813 648 549 375 359 359
159 48
MspA1I CmG'CkG
Cuts at: 1606 3964 4819 5595 6272 7420 7665 1606
Size: 2358 855 776 677 1148 245 2120
Fragments arranged by size:
2358 2120 1148 855 776 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7627
NdeI CA'TA_TG
Cuts at: 387 3748 5388 387
Size: 3361 1640 3178
Fragments arranged by size:
3361 3178 1640
NheI G'CTAG_C
Cuts at: 1052 2350 3017 1052
Size: 1298 667 6214
Fragments arranged by size:
6214 1298 667
NotI GC'GGCC_GC
Cuts at: 5626 5626
Size: 8179
NsiI A_TGCA'T
Cuts at: 2085 2085
Size: 8179
NspI r_CATG'y
Cuts at: 1132 1229 2239 5218 5537 7082 1132
Size: 97 1010 2979 319 1545 2229
Fragments arranged by size:
2979 2229 1545 1010 319 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8179
PciI A'CATG_T
Cuts at: 7078 7078
Size: 8179
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3185 3802 5084 5133 1108
Size: 2077 617 1282 49 4154
Fragments arranged by size:
4154 2077 1282 617 49
PmeI GTTT'AAAC
Cuts at: 5483 5483
Size: 8179
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6248 6280 7761 7793 6248
Size: 32 1481 32 6634
Fragments arranged by size:
6634 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4082 4680 5123 4082
Size: 598 443 7138
Fragments arranged by size:
7138 598 443
PshAI GACnn'nnGTC
Cuts at: 1613 4515 1613
Size: 2902 5277
PsiI TTA'TAA
Cuts at: 3090 5749 3090
Size: 2659 5520
PstI C_TGCA'G
Cuts at: 830 2439 3758 5341 5531 830
Size: 1609 1319 1583 190 3478
Fragments arranged by size:
3478 1609 1583 1319 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8179
PvuII CAG'CTG
Cuts at: 3964 4819 3964
Size: 855 7324
SalI G'TCGA_C
Cuts at: 1936 5521 1936
Size: 3585 4594
SapI GCTCTTCn'nnn_
Cuts at: 2013 3936 4692 2013
Size: 1923 756 5500
Fragments arranged by size:
5500 1923 756
SbfI CC_TGCA'GG
Cuts at: 5531 5531
Size: 8179
ScaI AGT'ACT
Cuts at: 3760 4216 4671 6427 8139 3760
Size: 456 455 1756 1712 3800
Fragments arranged by size:
3800 1756 1712 456 455
SfcI C'TryA_G
Cuts at: 826 1045 2435 3033 3754 5337 5527 6662 7343
Size: 219 1390 598 721 1583 190 1135 681
Cuts at: 7343 7534 826
Size: 191 1471
Fragments arranged by size:
1583 1471 1390 1135 721 681 598 219
191 190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8179
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8179
SmaI CCC'GGG
Cuts at: 1872 5506 1872
Size: 3634 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 2844 4201 4318 6283 7184
Size: 197 926 531 370 1357 117 1965 901
Cuts at: 7184 7446 820
Size: 262 1553
Fragments arranged by size:
1965 1553 1357 926 901 531 370 262
197 117
SnaBI TAC'GTA
Cuts at: 493 5970 493
Size: 5477 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8179
SphI G_CATG'C
Cuts at: 1132 2239 5537 1132
Size: 1107 3298 3774
Fragments arranged by size:
3774 3298 1107
SspI AAT'ATT
Cuts at: 5 52 3254 6103 5
Size: 47 3202 2849 2081
Fragments arranged by size:
3202 2849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8179
StyI C'CwwG_G
Cuts at: 513 1065 5618 513
Size: 552 4553 3074
Fragments arranged by size:
4553 3074 552
SwaI ATTT'AAAT
Cuts at: 4732 4732
Size: 8179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5088 6206 6223 6376 6561 7006 325
Size: 1255 3508 1118 17 153 185 445 1498
Fragments arranged by size:
3508 1498 1255 1118 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3230 3568 3635
Size: 274 80 33 51 1443 1253 338 67
Cuts at: 3635 3758 4214 4669 4959 6425 8137 96
Size: 123 456 455 290 1466 1712 138
Fragments arranged by size:
1712 1466 1443 1253 456 455 338 290
274 138 123 80 67 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 3403 3435 8139 8171 2516
Size: 32 855 32 4704 32 2524
Fragments arranged by size:
4704 2524 855 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3192 1316
Size: 1876 6303
XbaI T'CTAG_A
Cuts at: 4543 5072 5515 4543
Size: 529 443 7207
Fragments arranged by size:
7207 529 443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2936 4984 2936
Size: 2048 6131
XhoI C'TCGA_G
Cuts at: 1943 2844 1943
Size: 901 7278
XmaI C'CCGG_G
Cuts at: 1870 5504 1870
Size: 3634 4545
XmnI GAAnn'nnTTC
Cuts at: 6308 6308
Size: 8179
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5987 276
Size: 53 83 186 936 4453 2468
Fragments arranged by size:
4453 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI
BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI