Restriction map for
pFN21AB9096
(Circular) (Six-base) MAPSORT of: pFN21AB9096.seq Check: 815 from: 1 to: 6511
pFN21AB9096 6511 bp
With 182 enzymes: SgfI *
June 11, 2012 14:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4321 278
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3854 1937
Size: 1917 4594
Acc65I G'GTAC_C
Cuts at: 3832 3832
Size: 6511
AclI AA'CG_TT
Cuts at: 1563 4638 5011 1563
Size: 3075 373 3063
Fragments arranged by size:
3075 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 2831 3573 3646 4574 5958 6226 1632
Size: 932 267 742 73 928 1384 268 1917
Fragments arranged by size:
1917 1384 932 928 742 268 267 73
AfeI AGC'GCT
Cuts at: 3101 3101
Size: 6511
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6314
AflIII A'CryG_T
Cuts at: 1203 2439 3258 5410 6387 1203
Size: 1236 819 2152 977 1327
Fragments arranged by size:
2152 1327 1236 977 819
AgeI A'CCGG_T
Cuts at: 5342 5342
Size: 6511
AhdI GACnn_n'nnGTC
Cuts at: 5240 5240
Size: 6511
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6511
AlwNI CAG_nnn'CTG
Cuts at: 2143 2857 4254 5826 2143
Size: 714 1397 1572 2828
Fragments arranged by size:
2828 1572 1397 714
ApaI G_GGCC'C
Cuts at: 2873 2873
Size: 6511
ApaLI G'TGCA_C
Cuts at: 4568 5724 4568
Size: 1156 5355
ApoI r'AATT_y
Cuts at: 1493 3820 4046 4198 6307 1493
Size: 2327 226 152 2109 1697
Fragments arranged by size:
2327 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5065 160
Size: 872 4033 1606
Fragments arranged by size:
4033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
AvaI C'yCGr_G
Cuts at: 1870 1943 2578 3112 3836 1870
Size: 73 635 534 724 4545
Fragments arranged by size:
4545 724 635 534 73
BamHI G'GATC_C
Cuts at: 3792 3841 4208 3792
Size: 49 367 6095
Fragments arranged by size:
6095 367 49
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 3152 3323 3832 5287
Size: 325 217 627 397 968 171 509 1455
Cuts at: 5287 618
Size: 1842
Fragments arranged by size:
1842 1455 968 627 509 397 325 217
171
BanII G_rGCy'C
Cuts at: 1391 2747 2873 3036 3783 3830 1391
Size: 1356 126 163 747 47 4072
Fragments arranged by size:
4072 1356 747 163 126 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2262 2779 3430 928
Size: 971 363 517 651 4009
Fragments arranged by size:
4009 971 651 517 363
BbvCI CC'TCA_GC
Cuts at: 2060 2060
Size: 6511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4702 4736 1903
Size: 34 2765 34 3678
Fragments arranged by size:
3678 2765 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3076 4403 5613 3076
Size: 1327 1210 3974
Fragments arranged by size:
3974 1327 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6511
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5122 136
Size: 107 122 71 631 605 3450 1525
Fragments arranged by size:
3450 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6506 6506
Size: 6511
BlpI GC'TnA_GC
Cuts at: 3035 3122 3928 3035
Size: 87 806 5618
Fragments arranged by size:
5618 806 87
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2873 3155 4572 5728 1790
Size: 397 686 282 1417 1156 2573
Fragments arranged by size:
2573 1417 1156 686 397 282
BmgBI CAC'GTC
Cuts at: 1318 2440 2832 1318
Size: 1122 392 4997
Fragments arranged by size:
4997 1122 392
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2764 2875 3315
Size: 416 53 83 399 24 1347 111 440
Cuts at: 3315 3377 4242 5200 442
Size: 62 865 958 1753
Fragments arranged by size:
1753 1347 958 865 440 416 399 111
83 62 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2000 2846 3654 5171 1239
Size: 761 846 808 1517 2579
Fragments arranged by size:
2579 1517 846 808 761
Bpu10I CC'TnA_GC
Cuts at: 2060 2615 2060
Size: 555 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4636 5501 5799 4636
Size: 865 298 5348
Fragments arranged by size:
5348 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5174 882
Size: 617 3675 2219
Fragments arranged by size:
3675 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2326 3259 3652 4302 493
Size: 711 1122 933 393 650 2702
Fragments arranged by size:
2702 1122 933 711 650 393
BsaBI GATnn'nnATC
Cuts at: 2159 3164 6505 2159
Size: 1005 3341 2165
Fragments arranged by size:
3341 2165 1005
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3758 4318 4700 5335
Size: 53 83 186 936 2225 560 382 635
Cuts at: 5335 275
Size: 1451
Fragments arranged by size:
2225 1451 936 635 560 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2488 4944 5342 5616 5763 6174 2488
Size: 2456 398 274 147 411 2825
Fragments arranged by size:
2825 2456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2238 2268 2546 2576 2750
Size: 30 165 30 835 30 278 30 174
Cuts at: 2750 2780 1178
Size: 30 4909
Fragments arranged by size:
4909 835 278 174 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2570 2573 3515 3689 1186
Size: 1168 216 3 942 174 4008
Fragments arranged by size:
4008 1168 942 216 174 3
BseYI C'CCAG_C
Cuts at: 2382 2403 2894 3181 3418 5714 6210 2382
Size: 21 491 287 237 2296 496 2683
Fragments arranged by size:
2683 2296 496 491 287 237 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2804 3110 1163
Size: 573 1068 306 4564
Fragments arranged by size:
4564 1068 573 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3108 3961 4722 5750 1540
Size: 275 186 1107 853 761 1028 2301
Fragments arranged by size:
2301 1107 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2171 3123 3551 4572 4657 5728 2171
Size: 952 428 1021 85 1071 2954
Fragments arranged by size:
2954 1071 1021 952 428 85
BsmI GAATG_Cn'
Cuts at: 3340 4022 4115 3340
Size: 682 93 5736
Fragments arranged by size:
5736 682 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2822 3115 2822
Size: 293 6218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2187 2747 2873 3036 3123 3155
Size: 399 381 16 560 126 163 87 32
Cuts at: 3155 3551 3783 3830 4572 4657 5728 1391
Size: 396 232 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 560 399 396 381 232
163 126 87 85 47 32 16
BspEI T'CCGG_A
Cuts at: 6174 6174
Size: 6511
BspHI T'CATG_A
Cuts at: 2904 4400 6130 2904
Size: 1496 1730 3285
Fragments arranged by size:
3285 1730 1496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2828 3866 844
Size: 1984 1038 3489
Fragments arranged by size:
3489 1984 1038
BsrBI CCG'CTC
Cuts at: 1394 1823 4398 1394
Size: 429 2575 3507
Fragments arranged by size:
3507 2575 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3213 5006 5180 65
Size: 1183 1965 1793 174 1396
Fragments arranged by size:
1965 1793 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3238 3522 5155 5342 1700
Size: 110 1428 284 1633 187 2869
Fragments arranged by size:
2869 1633 1428 284 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6511
BssHII G'CGCG_C
Cuts at: 1925 2349 1925
Size: 424 6087
BssSI C'ACGA_G
Cuts at: 4571 5583 4571
Size: 1012 5499
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2413 3452 794
Size: 1619 1039 3853
Fragments arranged by size:
3853 1619 1039
BstBI TT'CG_AA
Cuts at: 5315 5315
Size: 6511
BstEII G'GTnAC_C
Cuts at: 1184 2212 1184
Size: 1028 5483
BstXI CCAn_nnnn'nTGG
Cuts at: 3772 3772
Size: 6511
BstYI r'GATC_y
Cuts at: 1972 2971 3792 3841 4208 4593 4610 6062 6148
Size: 999 821 49 367 385 17 1452 86
Cuts at: 6148 6160 6506 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 999 821 385 367 346 86
49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2038 3736 513
Size: 552 939 34 1698 3288
Fragments arranged by size:
3288 1698 939 552 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5772
Fragments arranged by size:
5772 683 56
BtsI GCAGTG_nn'
Cuts at: 3549 4027 4821 4841 3549
Size: 478 794 20 5219
Fragments arranged by size:
5219 794 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6476
DraI TTT'AAA
Cuts at: 3815 3815
Size: 6511
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5518 809
Size: 507 4202 1802
Fragments arranged by size:
4202 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2700 3003 3105
Size: 54 1425 50 275 18 870 303 102
Cuts at: 3105 3958 4847 6476 8
Size: 853 889 1629 43
Fragments arranged by size:
1629 1425 889 870 853 303 275 102
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3105 3958 1537
Size: 275 1293 853 4090
Fragments arranged by size:
4090 1293 853 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2396 2426 4441 1353
Size: 1043 30 2015 3423
Fragments arranged by size:
3423 2015 1043 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2737 3303 3722 5098 5472 5618
Size: 1064 138 1148 566 419 1376 374 146
Cuts at: 5618 387
Size: 1280
Fragments arranged by size:
1376 1280 1148 1064 566 419 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2000 2564 2831 2846 3573 3646 3654
Size: 393 368 564 267 15 727 73 8
Cuts at: 3654 4574 5171 5958 6226 1239
Size: 920 597 787 268 1524
Fragments arranged by size:
1524 920 787 727 597 564 393 368
268 267 73 15 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2192 2135
Size: 57 6454
EcoO109I rG'GnC_Cy
Cuts at: 2431 2869 2870 2431
Size: 438 1 6072
Fragments arranged by size:
6072 438 1
EcoRI G'AATT_C
Cuts at: 3820 4198 3820
Size: 378 6133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 6511
FspI TGC'GCA
Cuts at: 5017 5017
Size: 6511
HaeII r_GCGC'y
Cuts at: 1678 2390 3103 5658 1678
Size: 712 713 2555 2531
Fragments arranged by size:
2555 2531 713 712
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 2192 2224 2546
Size: 32 135 32 514 32 74 32 322
Cuts at: 2546 2578 5152 5184 5226 5258 1373
Size: 32 2574 32 42 32 2626
Fragments arranged by size:
2626 2574 514 322 135 74 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2451 2692 3855 4101 669
Size: 988 281 513 241 1163 246 3079
Fragments arranged by size:
3079 1163 988 513 281 246 241
HindIII A'AGCT_T
Cuts at: 748 3488 748
Size: 2740 3771
HpaI GTT'AAC
Cuts at: 4101 4101
Size: 6511
KpnI G_GTAC'C
Cuts at: 3836 3836
Size: 6511
MfeI C'AATT_G
Cuts at: 4110 4110
Size: 6511
MluI A'CGCG_T
Cuts at: 6387 6387
Size: 6511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5625 5809 1305
Size: 4320 184 2007
Fragments arranged by size:
4320 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2702 3005 10
Size: 54 1425 343 870 303 3516
Fragments arranged by size:
3516 1425 870 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2903 3770 4469 4828 4987 518
Size: 648 1122 615 867 699 359 159 2042
Fragments arranged by size:
2042 1122 867 699 648 615 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 3181 3329 3724 3927 4604 5752 5997
Size: 738 837 148 395 203 677 1148 245
Cuts at: 5997 1606
Size: 2120
Fragments arranged by size:
2120 1148 837 738 677 395 245 203
148
NaeI GCC'GGC
Cuts at: 3524 3524
Size: 6511
NcoI C'CATG_G
Cuts at: 513 1065 2004 2038 513
Size: 552 939 34 4986
Fragments arranged by size:
4986 939 552 34
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6511
NgoMIV G'CCGG_C
Cuts at: 3522 3522
Size: 6511
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6511
NotI GC'GGCC_GC
Cuts at: 3958 3958
Size: 6511
NspI r_CATG'y
Cuts at: 1132 1229 2280 2902 3427 3869 5414 1132
Size: 97 1051 622 525 442 1545 2229
Fragments arranged by size:
2229 1545 1051 622 525 442 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6511
PciI A'CATG_T
Cuts at: 5410 5410
Size: 6511
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6511
PfoI T'CCnGG_A
Cuts at: 2016 2479 2016
Size: 463 6048
PmeI GTTT'AAAC
Cuts at: 3815 3815
Size: 6511
PmlI CAC'GTG
Cuts at: 2326 3259 3652 2326
Size: 933 393 5185
Fragments arranged by size:
5185 933 393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 6093 6125 4580
Size: 32 1481 32 4966
Fragments arranged by size:
4966 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2431
Size: 6511
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6511
PsiI TTA'TAA
Cuts at: 4081 4081
Size: 6511
PspOMI G'GGCC_C
Cuts at: 2869 2869
Size: 6511
PstI C_TGCA'G
Cuts at: 830 2343 2862 3499 3863 830
Size: 1513 519 637 364 3478
Fragments arranged by size:
3478 1513 637 519 364
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6511
PvuII CAG'CTG
Cuts at: 3181 3724 3181
Size: 543 5968
SalI G'TCGA_C
Cuts at: 1936 3853 1936
Size: 1917 4594
SapI GCTCTTCn'nnn_
Cuts at: 2396 2426 2396
Size: 30 6481
SbfI CC_TGCA'GG
Cuts at: 2862 3863 2862
Size: 1001 5510
ScaI AGT'ACT
Cuts at: 4759 6471 4759
Size: 1712 4799
SfcI C'TryA_G
Cuts at: 826 1045 2253 2339 2858 3495 3597 3859 4994
Size: 219 1208 86 519 637 102 262 1135
Cuts at: 4994 5675 5866 826
Size: 681 191 1471
Fragments arranged by size:
1471 1208 1135 681 637 519 262 219
191 102 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6511
SmaI CCC'GGG
Cuts at: 1872 2580 3838 1872
Size: 708 1258 4545
Fragments arranged by size:
4545 1258 708
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4615 5516 5778 820
Size: 197 926 2672 901 262 1553
Fragments arranged by size:
2672 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4302 493
Size: 3809 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6511
SphI G_CATG'C
Cuts at: 1132 2902 3427 3869 1132
Size: 1770 525 442 3774
Fragments arranged by size:
3774 1770 525 442
SspI AAT'ATT
Cuts at: 5 52 2522 4435 5
Size: 47 2470 1913 2081
Fragments arranged by size:
2470 2081 1913 47
StuI AGG'CCT
Cuts at: 1517 3317 1517
Size: 1800 4711
StyI C'CwwG_G
Cuts at: 513 1065 2004 2038 3027 3950 513
Size: 552 939 34 989 923 3074
Fragments arranged by size:
3074 989 939 923 552 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2417 4538 4555 4708 4893 5338 325
Size: 1255 837 2121 17 153 185 445 1498
Fragments arranged by size:
2121 1498 1255 837 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4757 6469 96
Size: 274 80 33 51 1443 2780 1712 138
Fragments arranged by size:
2780 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2475 2507 3357 3389 6471 6503 2475
Size: 32 850 32 3082 32 2483
Fragments arranged by size:
3082 2483 850 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6511
XbaI T'CTAG_A
Cuts at: 3847 3847
Size: 6511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2666 2710 3772 2666
Size: 44 1062 5405
Fragments arranged by size:
5405 1062 44
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6511
XmaI C'CCGG_G
Cuts at: 1870 2578 3836 1870
Size: 708 1258 4545
Fragments arranged by size:
4545 1258 708
XmnI GAAnn'nnTTC
Cuts at: 4640 4640
Size: 6511
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4319 276
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI