Restriction map for
pFN21AB9055
(Circular) (Six-base) MAPSORT of: pFN21AB9055.seq Check: 4624 from: 1 to: 7639
pFN21AB9055 7639 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5449 278
Size: 53 83 186 936 3913 2468
Fragments arranged by size:
3913 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4982 1937
Size: 3045 4594
Acc65I G'GTAC_C
Cuts at: 2404 4960 2404
Size: 2556 5083
AclI AA'CG_TT
Cuts at: 1563 4077 5766 6139 1563
Size: 2514 1689 373 3063
Fragments arranged by size:
3063 2514 1689 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2766 2829 2886 3174 3285 3420 3612 5702
Size: 1134 63 57 288 111 135 192 2090
Cuts at: 5702 7086 7354 1632
Size: 1384 268 1917
Fragments arranged by size:
2090 1917 1384 1134 288 268 192 135
111 63 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7442
AflIII A'CryG_T
Cuts at: 1203 2163 2836 3269 6538 7515 1203
Size: 960 673 433 3269 977 1327
Fragments arranged by size:
3269 1327 977 960 673 433
AgeI A'CCGG_T
Cuts at: 6470 6470
Size: 7639
AhdI GACnn_n'nnGTC
Cuts at: 6368 6368
Size: 7639
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7639
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7639
AlwNI CAG_nnn'CTG
Cuts at: 2615 5382 6954 2615
Size: 2767 1572 3300
Fragments arranged by size:
3300 2767 1572
ApaLI G'TGCA_C
Cuts at: 5696 6852 5696
Size: 1156 6483
ApoI r'AATT_y
Cuts at: 1493 3159 3189 3262 3374 3425 3548 3689 3819
Size: 1666 30 73 112 51 123 141 130
Cuts at: 3819 4429 4580 4754 4948 5174 5326 7435 1493
Size: 610 151 174 194 226 152 2109 1697
Fragments arranged by size:
2109 1697 1666 610 226 194 174 152
151 141 130 123 112 73 51 30
AseI AT'TA_AT
Cuts at: 160 1032 4791 6193 160
Size: 872 3759 1402 1606
Fragments arranged by size:
3759 1606 1402 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7639
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 2489 4675 4964 1870
Size: 73 150 396 2186 289 4545
Fragments arranged by size:
4545 2186 396 289 150 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2388 2421 2388
Size: 33 7606
BamHI G'GATC_C
Cuts at: 4969 5336 4969
Size: 367 7272
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2404 4213 4960 6415 618
Size: 325 217 627 617 1809 747 1455 1842
Fragments arranged by size:
1842 1809 1455 747 627 617 325 217
BanII G_rGCy'C
Cuts at: 1391 4415 4958 1391
Size: 3024 543 4072
Fragments arranged by size:
4072 3024 543
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4251 4294 4476 928
Size: 971 2352 43 182 4091
Fragments arranged by size:
4091 2352 971 182 43
BbvCI CC'TCA_GC
Cuts at: 4039 4039
Size: 7639
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5830 5864 1903
Size: 34 3893 34 3678
Fragments arranged by size:
3893 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5531 6741 5531
Size: 1210 6429
BclI T'GATC_A
Cuts at: 1548 4807 1548
Size: 3259 4380
BfrBI ATG'CAT
Cuts at: 2275 2275
Size: 7639
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 6250 136
Size: 107 122 71 631 605 463 4115 1525
Fragments arranged by size:
4115 1525 631 605 463 122 107 71
BglII A'GATC_T
Cuts at: 3130 7634 3130
Size: 4504 3135
BlpI GC'TnA_GC
Cuts at: 5056 5056
Size: 7639
Bme1580I G_kGCm'C
Cuts at: 1790 5700 6856 1790
Size: 3910 1156 2573
Fragments arranged by size:
3910 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2164 2192 1318
Size: 846 28 6765
Fragments arranged by size:
6765 846 28
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5370 6328 442
Size: 416 53 83 399 24 3953 958 1753
Fragments arranged by size:
3953 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7639
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 4417 2068
Size: 2349 5290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2619 3221 4337 6299 1239
Size: 1380 602 1116 1962 2579
Fragments arranged by size:
2579 1962 1380 1116 602
Bpu10I CC'TnA_GC
Cuts at: 4039 4039
Size: 7639
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3051 3681 5764 6629 6927 3051
Size: 630 2083 865 298 3763
Fragments arranged by size:
3763 2083 865 630 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2427 2554 2908 6302 882
Size: 617 928 127 354 3394 2219
Fragments arranged by size:
3394 2219 928 617 354 127
BsaAI yAC'GTr
Cuts at: 493 1204 3029 3272 3454 4935 5430 493
Size: 711 1825 243 182 1481 495 2702
Fragments arranged by size:
2702 1825 1481 711 495 243 182
BsaBI GATnn'nnATC
Cuts at: 2748 7633 2748
Size: 4885 2754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5446 5828 6463 275
Size: 53 83 186 936 3913 382 635 1451
Fragments arranged by size:
3913 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2464 3088 6072 6470 6744 6891 7302 2464
Size: 624 2984 398 274 147 411 2801
Fragments arranged by size:
2984 2801 624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3749 3779 1178
Size: 30 165 30 2346 30 5038
Fragments arranged by size:
5038 2346 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 4393 1186
Size: 874 2333 4432
Fragments arranged by size:
4432 2333 874
BseYI C'CCAG_C
Cuts at: 2966 4697 6842 7338 2966
Size: 1731 2145 496 3267
Fragments arranged by size:
3267 2145 1731 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2474 1163
Size: 573 738 6328
Fragments arranged by size:
6328 738 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5089 5850 6878 1540
Size: 275 186 3088 761 1028 2301
Fragments arranged by size:
3088 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3398 4400 5700 5785 6856 3398
Size: 1002 1300 85 1071 4181
Fragments arranged by size:
4181 1300 1071 1002 85
BsmI GAATG_Cn'
Cuts at: 3013 4129 4305 5150 5243 3013
Size: 1116 176 845 93 5409
Fragments arranged by size:
5409 1116 845 176 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4373 4373
Size: 7639
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3398 4400 4415 4958 5700 5785 6856
Size: 399 1608 1002 15 543 742 85 1071
Cuts at: 6856 1391
Size: 2174
Fragments arranged by size:
2174 1608 1071 1002 742 543 399 85
15
BspEI T'CCGG_A
Cuts at: 2464 7302 2464
Size: 4838 2801
BspHI T'CATG_A
Cuts at: 5528 7258 5528
Size: 1730 5909
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4994 844
Size: 4150 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5526 1394
Size: 429 3703 3507
Fragments arranged by size:
3703 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2025 2142 2797 6134 6308 65
Size: 1183 777 117 655 3337 174 1396
Fragments arranged by size:
3337 1396 1183 777 655 174 117
BsrFI r'CCGG_y
Cuts at: 1700 1810 6283 6470 1700
Size: 110 4473 187 2869
Fragments arranged by size:
4473 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7639
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7639
BssSI C'ACGA_G
Cuts at: 4092 5699 6711 4092
Size: 1607 1012 5020
Fragments arranged by size:
5020 1607 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7639
BstBI TT'CG_AA
Cuts at: 4048 6443 4048
Size: 2395 5244
BstEII G'GTnAC_C
Cuts at: 1184 4000 1184
Size: 2816 4823
BstXI CCAn_nnnn'nTGG
Cuts at: 4705 4705
Size: 7639
BstYI r'GATC_y
Cuts at: 1972 2737 3130 4728 4969 5336 5721 5738 7190
Size: 765 393 1598 241 367 385 17 1452
Cuts at: 7190 7276 7288 7634 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1598 1452 765 393 385 367 346
241 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4269 4269
Size: 7639
BtgI C'CryG_G
Cuts at: 513 1065 2004 3844 4005 513
Size: 552 939 1840 161 4147
Fragments arranged by size:
4147 1840 939 552 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6900
Fragments arranged by size:
6900 683 56
BtsI GCAGTG_nn'
Cuts at: 2154 5155 5949 5969 2154
Size: 3001 794 20 3824
Fragments arranged by size:
3824 3001 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7639
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7604
DraI TTT'AAA
Cuts at: 2482 4943 2482
Size: 2461 5178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2168 3963 6646 809
Size: 507 852 1795 2683 1802
Fragments arranged by size:
2683 1802 1795 852 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 5086 5975
Size: 54 1425 50 275 18 285 2971 889
Cuts at: 5975 7604 8
Size: 1629 43
Fragments arranged by size:
2971 1629 1425 889 285 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5086 1537
Size: 275 3274 4090
Fragments arranged by size:
4090 3274 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3666 4169 5569 1353
Size: 2313 503 1400 3423
Fragments arranged by size:
3423 2313 1400 503
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6226 6600 6746 387
Size: 1064 138 4637 374 146 1280
Fragments arranged by size:
4637 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2619 2766 2829 2886 3174 3221 3285
Size: 393 987 147 63 57 288 47 64
Cuts at: 3285 3420 3612 4337 5702 6299 7086 7354 1239
Size: 135 192 725 1365 597 787 268 1524
Fragments arranged by size:
1524 1365 987 787 725 597 393 288
268 192 147 135 64 63 57 47
EcoO109I rG'GnC_Cy
Cuts at: 2860 4013 4826 2860
Size: 1153 813 5673
Fragments arranged by size:
5673 1153 813
EcoRI G'AATT_C
Cuts at: 3159 3189 3262 3819 4948 5326 3159
Size: 30 73 557 1129 378 5472
Fragments arranged by size:
5472 1129 557 378 73 30
FspI TGC'GCA
Cuts at: 6145 6145
Size: 7639
HaeII r_GCGC'y
Cuts at: 1678 6786 1678
Size: 5108 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 3340 3372 4889
Size: 32 135 32 461 32 1275 32 1517
Cuts at: 4889 4921 6280 6312 6354 6386 1373
Size: 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1517 1359 1275 461 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4983 5229 669
Size: 988 281 3045 246 3079
Fragments arranged by size:
3079 3045 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2382 3936 748
Size: 1634 1554 4451
Fragments arranged by size:
4451 1634 1554
HpaI GTT'AAC
Cuts at: 5229 5229
Size: 7639
KpnI G_GTAC'C
Cuts at: 2408 4964 2408
Size: 2556 5083
MfeI C'AATT_G
Cuts at: 5238 5238
Size: 7639
MluI A'CGCG_T
Cuts at: 7515 7515
Size: 7639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2155 4556 4842 6753 6937 1305
Size: 850 2401 286 1911 184 2007
Fragments arranged by size:
2401 2007 1911 850 286 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 10
Size: 54 1425 343 285 5532
Fragments arranged by size:
5532 1425 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2589 2660 2804 3441 3530 4487
Size: 648 981 442 71 144 637 89 957
Cuts at: 4487 5597 5956 6115 518
Size: 1110 359 159 2042
Fragments arranged by size:
2042 1110 981 957 648 637 442 359
159 144 89 71
MspA1I CmG'CkG
Cuts at: 1606 2711 3846 4043 4801 4847 5055 5732 6880
Size: 1105 1135 197 758 46 208 677 1148
Cuts at: 6880 7125 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1135 1105 758 677 245 208
197 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6148
Fragments arranged by size:
6148 939 552
NdeI CA'TA_TG
Cuts at: 387 2416 387
Size: 2029 5610
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7639
NotI GC'GGCC_GC
Cuts at: 5086 5086
Size: 7639
NsiI A_TGCA'T
Cuts at: 2277 2277
Size: 7639
NspI r_CATG'y
Cuts at: 1132 1229 2588 2840 4672 4997 6542 1132
Size: 97 1359 252 1832 325 1545 2229
Fragments arranged by size:
2229 1832 1545 1359 325 252 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7639
PciI A'CATG_T
Cuts at: 2836 6538 2836
Size: 3702 3937
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7639
PfoI T'CCnGG_A
Cuts at: 2949 4239 4777 2949
Size: 1290 538 5811
Fragments arranged by size:
5811 1290 538
PmeI GTTT'AAAC
Cuts at: 4943 4943
Size: 7639
PmlI CAC'GTG
Cuts at: 3272 4935 3272
Size: 1663 5976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5708 5740 7221 7253 5708
Size: 32 1481 32 6094
Fragments arranged by size:
6094 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2860 2860
Size: 7639
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7639
PsiI TTA'TAA
Cuts at: 5209 5209
Size: 7639
PstI C_TGCA'G
Cuts at: 830 2269 4991 830
Size: 1439 2722 3478
Fragments arranged by size:
3478 2722 1439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7639
PvuII CAG'CTG
Cuts at: 2711 4043 4801 4847 2711
Size: 1332 758 46 5503
Fragments arranged by size:
5503 1332 758 46
SacII CC_GC'GG
Cuts at: 3847 3847
Size: 7639
SalI G'TCGA_C
Cuts at: 1936 4981 1936
Size: 3045 4594
SbfI CC_TGCA'GG
Cuts at: 4991 4991
Size: 7639
ScaI AGT'ACT
Cuts at: 5887 7599 5887
Size: 1712 5927
SexAI A'CCwGG_T
Cuts at: 3809 3809
Size: 7639
SfcI C'TryA_G
Cuts at: 826 1045 2265 2322 4624 4987 6122 6803 6994
Size: 219 1220 57 2302 363 1135 681 191
Cuts at: 6994 826
Size: 1471
Fragments arranged by size:
2302 1471 1220 1135 681 363 219 191
57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7639
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7639
SmaI CCC'GGG
Cuts at: 1872 4677 4966 1872
Size: 2805 289 4545
Fragments arranged by size:
4545 2805 289
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2093 3066 3696 5743 6644 6906
Size: 197 926 150 973 630 2047 901 262
Cuts at: 6906 820
Size: 1553
Fragments arranged by size:
2047 1553 973 926 901 630 262 197
150
SnaBI TAC'GTA
Cuts at: 493 3029 5430 493
Size: 2536 2401 2702
Fragments arranged by size:
2702 2536 2401
SpeI A'CTAG_T
Cuts at: 152 3886 152
Size: 3734 3905
SphI G_CATG'C
Cuts at: 1132 4672 4997 1132
Size: 3540 325 3774
Fragments arranged by size:
3774 3540 325
SspI AAT'ATT
Cuts at: 5 52 2240 5563 5
Size: 47 2188 3323 2081
Fragments arranged by size:
3323 2188 2081 47
StuI AGG'CCT
Cuts at: 1517 4118 1517
Size: 2601 5038
StyI C'CwwG_G
Cuts at: 513 1065 2004 3387 3405 3509 5078 513
Size: 552 939 1383 18 104 1569 3074
Fragments arranged by size:
3074 1569 1383 939 552 104 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4453 5666 5683 5836 6021 6466 325
Size: 1255 2873 1213 17 153 185 445 1498
Fragments arranged by size:
2873 1498 1255 1213 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5885 7597 96
Size: 274 80 33 51 1443 3908 1712 138
Fragments arranged by size:
3908 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4570 4602 7599 7631 4570
Size: 32 2997 32 4578
Fragments arranged by size:
4578 2997 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7639
XbaI T'CTAG_A
Cuts at: 4975 4975
Size: 7639
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 4932 2131
Size: 2801 4838
XhoI C'TCGA_G
Cuts at: 1943 2093 1943
Size: 150 7489
XmaI C'CCGG_G
Cuts at: 1870 4675 4964 1870
Size: 2805 289 4545
Fragments arranged by size:
4545 2805 289
XmnI GAAnn'nnTTC
Cuts at: 3147 3159 3228 3474 5768 3147
Size: 12 69 246 2294 5018
Fragments arranged by size:
5018 2294 246 69 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5447 276
Size: 53 83 186 936 3913 2468
Fragments arranged by size:
3913 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI
PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI
SwaI