Restriction map for
pFN21AB9044
(Circular) (Six-base) MAPSORT of: pFN21AB9044.seq Check: 8891 from: 1 to: 6295
pFN21AB9044 6295 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3638 1937
Size: 1701 4594
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2298 4358 5742 6010 1632
Size: 666 2060 1384 268 1917
Fragments arranged by size:
2060 1917 1384 666 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 5194 6171 1203
Size: 3991 977 1327
Fragments arranged by size:
3991 1327 977
AgeI A'CCGG_T
Cuts at: 3504 5126 3504
Size: 1622 4673
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AlwNI CAG_nnn'CTG
Cuts at: 4038 5610 4038
Size: 1572 4723
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2066 2110 2359 2498 2506 2610 2626 2694
Size: 573 44 249 139 8 104 16 68
Cuts at: 2694 2778 2946 3030 3114 3198 3282 3450 3534
Size: 84 168 84 84 84 84 168 84
Cuts at: 3534 3604 3830 3982 6091 1493
Size: 70 226 152 2109 1697
Fragments arranged by size:
2109 1697 573 249 226 168 168 152
139 104 84 84 84 84 84 84
70 68 44 16 8
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 3620 1870
Size: 73 1677 4545
Fragments arranged by size:
4545 1677 73
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3616 5071 618
Size: 325 217 627 1829 1455 1842
Fragments arranged by size:
1842 1829 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 3614 1391
Size: 826 1397 4072
Fragments arranged by size:
4072 1397 826
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2146 2286 928
Size: 971 247 140 4937
Fragments arranged by size:
4937 971 247 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4486 4520 1903
Size: 34 50 34 2465 34 3678
Fragments arranged by size:
3678 2465 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4906 136
Size: 107 122 71 631 605 3234 1525
Fragments arranged by size:
3234 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 4356 5512 1790
Size: 2566 1156 2573
Fragments arranged by size:
2573 2566 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6295
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2249 4026 4984
Size: 416 53 83 399 24 832 1777 958
Cuts at: 4984 442
Size: 1753
Fragments arranged by size:
1777 1753 958 832 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2892 2976 3060 3144 3228 3244 3396
Size: 857 796 84 84 84 84 16 152
Cuts at: 3396 4955 1239
Size: 1559 2579
Fragments arranged by size:
2579 1559 857 796 152 84 84 84
84 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5285 5583 4420
Size: 865 298 5132
Fragments arranged by size:
5132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2482 4086 493
Size: 711 1278 1604 2702
Fragments arranged by size:
2702 1604 1278 711
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4102 4484 5119 275
Size: 53 83 186 936 2569 382 635 1451
Fragments arranged by size:
2569 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3504 4728 5126 5400 5547 5958 3504
Size: 1224 398 274 147 411 3841
Fragments arranged by size:
3841 1224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6070
Fragments arranged by size:
6070 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 1186
Size: 907 5388
BseYI C'CCAG_C
Cuts at: 5498 5994 5498
Size: 496 5799
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2085 1163
Size: 573 349 5373
Fragments arranged by size:
5373 573 349
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3260 4356 4441 5512 3260
Size: 1096 85 1071 4043
Fragments arranged by size:
4043 1096 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3806 3899 3155
Size: 651 93 5551
Fragments arranged by size:
5551 651 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 3260 3614 4356 4441 5512 1391
Size: 399 427 1043 354 742 85 1071 2174
Fragments arranged by size:
2174 1071 1043 742 427 399 354 85
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3650 844
Size: 2806 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2093 3415 4790 4964 65
Size: 1183 845 1322 1375 174 1396
Fragments arranged by size:
1396 1375 1322 1183 845 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3504 4939 5126 1700
Size: 110 1694 1435 187 2869
Fragments arranged by size:
2869 1694 1435 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3434 794
Size: 2640 3655
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 3625 3992 4377 4394 5846 5932 5944 6290
Size: 1653 367 385 17 1452 86 12 346
Cuts at: 6290 1972
Size: 1977
Fragments arranged by size:
1977 1653 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5743
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 3811 4605 4625 2097
Size: 1714 794 20 3767
Fragments arranged by size:
3767 1714 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2288 3742
Size: 54 1425 50 275 18 228 230 1454
Cuts at: 3742 4631 6260 8
Size: 889 1629 43
Fragments arranged by size:
1629 1454 1425 889 275 230 228 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2784 4225 1353
Size: 869 562 1441 3423
Fragments arranged by size:
3423 1441 869 562
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2298 2892 2976 3060 3144 3228
Size: 393 464 202 594 84 84 84 84
Cuts at: 3228 3244 3396 4358 4955 5742 6010 1239
Size: 16 152 962 597 787 268 1524
Fragments arranged by size:
1524 962 787 597 594 464 393 268
202 152 84 84 84 84 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2221 2221
Size: 6295
EcoRI G'AATT_C
Cuts at: 2066 2610 2626 2694 2946 3198 3282 3450 3604
Size: 544 16 68 252 252 84 168 154
Cuts at: 3604 3982 2066
Size: 378 4379
Fragments arranged by size:
4379 544 378 252 252 168 154 84
68 16
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 5442 1678
Size: 3764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4936 4968 5010 5042 1373
Size: 32 135 32 3364 32 42 32 2626
Fragments arranged by size:
3364 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3075 3159 3243 3495 748
Size: 2327 84 84 252 3548
Fragments arranged by size:
3548 2327 252 84 84
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5409 5593 1305
Size: 4104 184 2007
Fragments arranged by size:
4104 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 2290 10
Size: 54 1425 343 228 230 4015
Fragments arranged by size:
4015 1425 343 230 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3543 4253 4612 4771 518
Size: 648 2377 710 359 159 2042
Fragments arranged by size:
2377 2042 710 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3711 4388 5536 5781 1606
Size: 2105 677 1148 245 2120
Fragments arranged by size:
2120 2105 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5743
NdeI CA'TA_TG
Cuts at: 387 2700 3192 387
Size: 2313 492 3490
Fragments arranged by size:
3490 2313 492
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2584 3653 5198 1132
Size: 97 1355 1069 1545 2229
Fragments arranged by size:
2229 1545 1355 1069 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 2421 3865 2421
Size: 1444 4851
PstI C_TGCA'G
Cuts at: 830 3647 830
Size: 2817 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SexAI A'CCwGG_T
Cuts at: 2148 2148
Size: 6295
SfcI C'TryA_G
Cuts at: 826 1045 2140 3643 4778 5459 5650 826
Size: 219 1095 1503 1135 681 191 1471
Fragments arranged by size:
1503 1471 1135 1095 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4399 5300 5562 820
Size: 197 926 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 2584 3653 1132
Size: 1452 1069 3774
Fragments arranged by size:
3774 1452 1069
SspI AAT'ATT
Cuts at: 5 52 2463 4219 5
Size: 47 2411 1756 2081
Fragments arranged by size:
2411 2081 1756 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 3734 513
Size: 552 2669 3074
Fragments arranged by size:
3074 2669 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3243 4322 4339 4492 4677 5122 325
Size: 1255 1663 1079 17 153 185 445 1498
Fragments arranged by size:
1663 1498 1255 1079 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4541 6253 96
Size: 274 80 33 51 1443 2564 1712 138
Fragments arranged by size:
2564 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6255 6287 6255
Size: 32 6263
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 2546 2798 2966 4424 2546
Size: 252 168 1458 4417
Fragments arranged by size:
4417 1458 252 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI