Restriction map for
pFN21AB9038
(Circular) (Six-base) MAPSORT of: pFN21AB9038.seq Check: 4674 from: 1 to: 6037
pFN21AB9038 6037 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 6037
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3847 278
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3380 1937
Size: 1443 4594
Acc65I G'GTAC_C
Cuts at: 3358 3358
Size: 6037
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2488 2669 4100 5484 5752 1632
Size: 856 181 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 856 268 181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 4936 5913 1203
Size: 3733 977 1327
Fragments arranged by size:
3733 1327 977
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 4766 4766
Size: 6037
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3177 1184
Size: 1993 4044
AlwNI CAG_nnn'CTG
Cuts at: 2438 3780 5352 2438
Size: 1342 1572 3123
Fragments arranged by size:
3123 1572 1342
ApaLI G'TGCA_C
Cuts at: 2018 4094 5250 2018
Size: 2076 1156 2805
Fragments arranged by size:
2805 2076 1156
ApoI r'AATT_y
Cuts at: 1493 3346 3572 3724 5833 1493
Size: 1853 226 152 2109 1697
Fragments arranged by size:
2109 1853 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4591 160
Size: 872 3559 1606
Fragments arranged by size:
3559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 3362 1870
Size: 73 1419 4545
Fragments arranged by size:
4545 1419 73
BamHI G'GATC_C
Cuts at: 3036 3367 3734 3036
Size: 331 367 5339
Fragments arranged by size:
5339 367 331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2105 2330 2512 3358
Size: 325 217 627 267 51 225 182 846
Cuts at: 3358 4813 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 846 627 325 267 225 217
182 51
BanII G_rGCy'C
Cuts at: 1391 2550 2854 3135 3356 1391
Size: 1159 304 281 221 4072
Fragments arranged by size:
4072 1159 304 281 221
BbeI G_GCGC'C
Cuts at: 2334 2334
Size: 6037
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2875 2944 928
Size: 971 976 69 4021
Fragments arranged by size:
4021 976 971 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2032 2066 4228 4262 1903
Size: 34 95 34 2162 34 3678
Fragments arranged by size:
3678 2162 95 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3929 5139 3929
Size: 1210 4827
BclI T'GATC_A
Cuts at: 1548 2073 1548
Size: 525 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2397 4648 136
Size: 107 122 71 631 605 725 2251 1525
Fragments arranged by size:
2251 1525 725 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2253 6032 2253
Size: 3779 2258
BlpI GC'TnA_GC
Cuts at: 3454 3454
Size: 6037
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2432 2450 4098 5254 1790
Size: 232 410 18 1648 1156 2573
Fragments arranged by size:
2573 1648 1156 410 232 18
BmgBI CAC'GTC
Cuts at: 1318 2789 1318
Size: 1471 4566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 2429 3768
Size: 416 53 83 399 24 897 115 1339
Cuts at: 3768 4726 442
Size: 958 1753
Fragments arranged by size:
1753 1339 958 897 416 399 115 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4697 1239
Size: 3458 2579
Bpu10I CC'TnA_GC
Cuts at: 2236 2236
Size: 6037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3071 4162 5027 5325 3071
Size: 1091 865 298 3783
Fragments arranged by size:
3783 1091 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 4700 882
Size: 617 1092 2109 2219
Fragments arranged by size:
2219 2109 1092 617
BsaAI yAC'GTr
Cuts at: 493 1204 3828 493
Size: 711 2624 2702
Fragments arranged by size:
2702 2624 711
BsaBI GATnn'nnATC
Cuts at: 6031 6031
Size: 6037
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2331 3844 4226 4861
Size: 53 83 186 936 798 1513 382 635
Cuts at: 4861 275
Size: 1451
Fragments arranged by size:
1513 1451 936 798 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4470 4868 5142 5289 5700 4470
Size: 398 274 147 411 4807
Fragments arranged by size:
4807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5812
Fragments arranged by size:
5812 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3314 3317 3332 3344 1186
Size: 2128 3 15 12 3879
Fragments arranged by size:
3879 2128 15 12 3
BseYI C'CCAG_C
Cuts at: 2874 5240 5736 2874
Size: 2366 496 3175
Fragments arranged by size:
3175 2366 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3023 1163
Size: 573 1287 4177
Fragments arranged by size:
4177 1287 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3487 4248 5276 1540
Size: 275 186 928 558 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 558 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2854 3135 4098 4183 5254 2022
Size: 832 281 963 85 1071 2805
Fragments arranged by size:
2805 1071 963 832 281 85
BsmI GAATG_Cn'
Cuts at: 3548 3641 3548
Size: 93 5944
BsmBI CGTCTCn'nnnn_
Cuts at: 2795 3265 2795
Size: 470 5567
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2432 2450 2550 2854 3135 3356
Size: 399 232 410 18 100 304 281 221
Cuts at: 3356 4098 4183 5254 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 304 281 232
221 100 85 18
BspEI T'CCGG_A
Cuts at: 5700 5700
Size: 6037
BspHI T'CATG_A
Cuts at: 2493 3926 5656 2493
Size: 1433 1730 2874
Fragments arranged by size:
2874 1730 1433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2285 2726 2869 3392 844
Size: 1292 149 441 143 523 3489
Fragments arranged by size:
3489 1292 523 441 149 143
BsrBI CCG'CTC
Cuts at: 1394 1823 3924 1394
Size: 429 2101 3507
Fragments arranged by size:
3507 2101 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4532 4706 65
Size: 1183 3284 174 1396
Fragments arranged by size:
3284 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 4681 4868 1700
Size: 110 292 2579 187 2869
Fragments arranged by size:
2869 2579 292 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6037
BssSI C'ACGA_G
Cuts at: 2187 3078 4097 5109 2187
Size: 891 1019 1012 3115
Fragments arranged by size:
3115 1019 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6037
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6037
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2200
Size: 6037
BstYI r'GATC_y
Cuts at: 1972 2203 2253 2466 2674 3036 3367 3734 4119
Size: 231 50 213 208 362 331 367 385
Cuts at: 4119 4136 5588 5674 5686 6032 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 362 346 331 231
213 208 86 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2113 2220 2538 2830 2923 2940 2956
Size: 552 1048 107 318 292 93 17 16
Cuts at: 2956 513
Size: 3594
Fragments arranged by size:
3594 1048 552 318 292 107 93 17
16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5298
Fragments arranged by size:
5298 683 56
BtsI GCAGTG_nn'
Cuts at: 3159 3553 4347 4367 3159
Size: 394 794 20 4829
Fragments arranged by size:
4829 794 394 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 3341 3341
Size: 6037
DraIII CAC_nnn'GTG
Cuts at: 3080 3080
Size: 6037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2540 5044 809
Size: 507 1224 2504 1802
Fragments arranged by size:
2504 1802 1224 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 2734 2926
Size: 54 1425 50 275 18 418 486 192
Cuts at: 2926 3484 4373 6002 8
Size: 558 889 1629 43
Fragments arranged by size:
1629 1425 889 558 486 418 275 192
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 3484 1537
Size: 275 1114 558 4090
Fragments arranged by size:
4090 1114 558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3140 3291 3321 3967 1353
Size: 1787 151 30 646 3423
Fragments arranged by size:
3423 1787 646 151 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3161 4624 4998 5144 387
Size: 1064 138 1572 1463 374 146 1280
Fragments arranged by size:
1572 1463 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2852 3133 2852
Size: 281 5756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2488 2669 4100 4697 5484 5752 1239
Size: 393 856 181 1431 597 787 268 1524
Fragments arranged by size:
1524 1431 856 787 597 393 268 181
EcoO109I rG'GnC_Cy
Cuts at: 2240 2620 2240
Size: 380 5657
EcoRI G'AATT_C
Cuts at: 3346 3724 3346
Size: 378 5659
EcoRV GAT'ATC
Cuts at: 2632 2632
Size: 6037
FspI TGC'GCA
Cuts at: 4543 4543
Size: 6037
HaeII r_GCGC'y
Cuts at: 1678 2334 5184 1678
Size: 656 2850 2531
Fragments arranged by size:
2850 2531 656
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 3245 3277 4678
Size: 32 135 32 557 32 1084 32 1401
Cuts at: 4678 4710 4752 4784 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1401 1084 557 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2770 3381 3627 669
Size: 988 281 832 611 246 3079
Fragments arranged by size:
3079 988 832 611 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KasI G'GCGC_C
Cuts at: 2330 2330
Size: 6037
KpnI G_GTAC'C
Cuts at: 3362 3362
Size: 6037
MfeI C'AATT_G
Cuts at: 3636 3636
Size: 6037
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2942 5151 5335 1305
Size: 1637 2209 184 2007
Fragments arranged by size:
2209 2007 1637 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2250 2736 10
Size: 54 1425 343 418 486 3311
Fragments arranged by size:
3311 1425 486 418 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2198 3164 3995 4354 4513 518
Size: 648 981 51 966 831 359 159 2042
Fragments arranged by size:
2042 981 966 831 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2134 2407 3453 4130 5278 5523 1606
Size: 528 273 1046 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 528 273 245
NarI GG'CG_CC
Cuts at: 2331 2331
Size: 6037
NcoI C'CATG_G
Cuts at: 513 1065 2220 513
Size: 552 1155 4330
Fragments arranged by size:
4330 1155 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6037
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 3395 4940 1132
Size: 97 2166 1545 2229
Fragments arranged by size:
2229 2166 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6037
PciI A'CATG_T
Cuts at: 4936 4936
Size: 6037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 2419 2578 3080 1108
Size: 956 355 159 502 4065
Fragments arranged by size:
4065 956 502 355 159
PfoI T'CCnGG_A
Cuts at: 2352 3126 2352
Size: 774 5263
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 4106 4138 5619 5651 3209
Size: 32 865 32 1481 32 3595
Fragments arranged by size:
3595 1481 865 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2240 2240
Size: 6037
PshAI GACnn'nnGTC
Cuts at: 1613 3059 1613
Size: 1446 4591
PsiI TTA'TAA
Cuts at: 3607 3607
Size: 6037
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 6005
PstI C_TGCA'G
Cuts at: 830 2133 2397 3389 830
Size: 1303 264 992 3478
Fragments arranged by size:
3478 1303 992 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
PvuII CAG'CTG
Cuts at: 2134 2407 2134
Size: 273 5764
SacI G_AGCT'C
Cuts at: 2854 3135 2854
Size: 281 5756
SalI G'TCGA_C
Cuts at: 1936 3379 1936
Size: 1443 4594
SapI GCTCTTCn'nnn_
Cuts at: 3140 3140
Size: 6037
SbfI CC_TGCA'GG
Cuts at: 2397 3389 2397
Size: 992 5045
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SfcI C'TryA_G
Cuts at: 826 1045 2129 2393 3385 4520 5201 5392 826
Size: 219 1084 264 992 1135 681 191 1471
Fragments arranged by size:
1471 1135 1084 992 681 264 219 191
SfoI GGC'GCC
Cuts at: 2332 2332
Size: 6037
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6037
SmaI CCC'GGG
Cuts at: 1872 3364 1872
Size: 1492 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3086 4141 5042 5304 820
Size: 197 926 1143 1055 901 262 1553
Fragments arranged by size:
1553 1143 1055 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SspI AAT'ATT
Cuts at: 5 52 3961 5
Size: 47 3909 2081
Fragments arranged by size:
3909 2081 47
StuI AGG'CCT
Cuts at: 1517 3182 1517
Size: 1665 4372
StyI C'CwwG_G
Cuts at: 513 1065 2220 3476 513
Size: 552 1155 1256 3074
Fragments arranged by size:
3074 1256 1155 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2536 2888 4064 4081 4234 4419 4864
Size: 1255 956 352 1176 17 153 185 445
Cuts at: 4864 325
Size: 1498
Fragments arranged by size:
1498 1255 1176 956 445 352 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 4283 5995
Size: 274 80 33 51 1443 174 2132 1712
Cuts at: 5995 96
Size: 138
Fragments arranged by size:
2132 1712 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5997 6029 5997
Size: 32 6005
Tth111I GACn'n_nGTC
Cuts at: 1316 3257 1316
Size: 1941 4096
XbaI T'CTAG_A
Cuts at: 2206 3373 2206
Size: 1167 4870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2899 2899
Size: 6037
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6037
XmaI C'CCGG_G
Cuts at: 1870 3362 1870
Size: 1492 4545
XmnI GAAnn'nnTTC
Cuts at: 2501 3182 4166 2501
Size: 681 984 4372
Fragments arranged by size:
4372 984 681
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3845 276
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoNI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacII SanDI SexAI SfiI SrfI SwaI