Restriction map for
pFN21AB9022
(Circular) (Six-base) MAPSORT of: pFN21AB9022.seq Check: 6149 from: 1 to: 9841
pFN21AB9022 9841 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7004 7004
Size: 9841
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7651 278
Size: 53 83 186 936 6115 2468
Fragments arranged by size:
6115 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7184 1937
Size: 5247 4594
Acc65I G'GTAC_C
Cuts at: 7162 7162
Size: 9841
AclI AA'CG_TT
Cuts at: 1563 7968 8341 1563
Size: 6405 373 3063
Fragments arranged by size:
6405 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4481 4895 5339 5375 5780 7904 9288 9556
Size: 2849 414 444 36 405 2124 1384 268
Cuts at: 9556 1632
Size: 1917
Fragments arranged by size:
2849 2124 1917 1384 444 414 405 268
36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9644
AflIII A'CryG_T
Cuts at: 1203 8740 9717 1203
Size: 7537 977 1327
Fragments arranged by size:
7537 1327 977
AgeI A'CCGG_T
Cuts at: 8672 8672
Size: 9841
AhdI GACnn_n'nnGTC
Cuts at: 6216 8570 6216
Size: 2354 7487
AleI CACnn'nnGTG
Cuts at: 1166 3995 1166
Size: 2829 7012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9841
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6677 6709 6677
Size: 32 9809
AlwNI CAG_nnn'CTG
Cuts at: 4139 5642 5723 5912 6595 6771 7026 7116 7584
Size: 1503 81 189 683 176 255 90 468
Cuts at: 7584 9156 4139
Size: 1572 4824
Fragments arranged by size:
4824 1572 1503 683 468 255 189 176
90 81
ApaI G_GGCC'C
Cuts at: 2207 2388 2451 2487 3039 3115 3275 3800 3863
Size: 181 63 36 552 76 160 525 63
Cuts at: 3863 3950 4059 4158 4302 4560 4929 5894 6003
Size: 87 109 99 144 258 369 965 109
Cuts at: 6003 6048 6267 6275 6321 2207
Size: 45 219 8 46 5727
Fragments arranged by size:
5727 965 552 525 369 258 219 181
160 144 109 109 99 87 76 63
63 46 45 36 8
ApaLI G'TGCA_C
Cuts at: 6408 7898 9054 6408
Size: 1490 1156 7195
Fragments arranged by size:
7195 1490 1156
ApoI r'AATT_y
Cuts at: 1493 2358 5296 7150 7376 7528 9637 1493
Size: 865 2938 1854 226 152 2109 1697
Fragments arranged by size:
2938 2109 1854 1697 865 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8395 160
Size: 872 7363 1606
Fragments arranged by size:
7363 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9841
AvaI C'yCGr_G
Cuts at: 1870 1943 2245 2371 2480 3003 3097 3151 3314
Size: 73 302 126 109 523 94 54 163
Cuts at: 3314 3707 4221 4346 4751 4823 6149 6167 6430
Size: 393 514 125 405 72 1326 18 263
Cuts at: 6430 7043 7166 1870
Size: 613 123 4545
Fragments arranged by size:
4545 1326 613 523 514 405 393 302
263 163 126 125 123 109 94 73
72 54 18
BamHI G'GATC_C
Cuts at: 4226 4262 5586 5630 7171 7538 4226
Size: 36 1324 44 1541 367 6529
Fragments arranged by size:
6529 1541 1324 367 44 36
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2348 3083 4100 5222 5333
Size: 325 217 627 561 735 1017 1122 111
Cuts at: 5333 5612 5839 5857 6143 6247 6992 7162 8617
Size: 279 227 18 286 104 745 170 1455
Cuts at: 8617 618
Size: 1842
Fragments arranged by size:
1842 1455 1122 1017 745 735 627 561
325 286 279 227 217 170 111 104
18
BanII G_rGCy'C
Cuts at: 1391 2207 2290 2388 2451 2487 2641 3039 3115
Size: 816 83 98 63 36 154 398 76
Cuts at: 3115 3275 3321 3415 3597 3750 3800 3863 3950
Size: 160 46 94 182 153 50 63 87
Cuts at: 3950 4059 4158 4302 4342 4543 4560 4929 5554
Size: 109 99 144 40 201 17 369 625
Cuts at: 5554 5761 5789 5894 5959 6003 6048 6166 6267
Size: 207 28 105 65 44 45 118 101
Cuts at: 6267 6275 6304 6321 6431 6510 6611 7064 7160
Size: 8 29 17 110 79 101 453 96
Cuts at: 7160 1391
Size: 4072
Fragments arranged by size:
4072 816 625 453 398 369 207 201
182 160 154 153 144 118 110 109
105 101 101 99 98 96 94 87
83 79 76 65 63 63 50 46
45 44 40 36 29 28 17 17
8
BbeI G_GCGC'C
Cuts at: 5861 5861
Size: 9841
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3479 5314 928
Size: 971 1580 1835 5455
Fragments arranged by size:
5455 1835 1580 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8032 8066 1903
Size: 34 6095 34 3678
Fragments arranged by size:
6095 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5006 5042 6539 7733 8943 5006
Size: 36 1497 1194 1210 5904
Fragments arranged by size:
5904 1497 1210 1194 36
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9841
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2482 2979 3573
Size: 107 122 71 631 605 810 497 594
Cuts at: 3573 3771 3882 3891 3954 4251 4297 4473 4500
Size: 198 111 9 63 297 46 176 27
Cuts at: 4500 4564 4644 5002 5086 5247 6522 8452 136
Size: 64 80 358 84 161 1275 1930 1525
Fragments arranged by size:
1930 1525 1275 810 631 605 594 497
358 297 198 176 161 122 111 107
84 80 71 64 63 46 27 9
BglII A'GATC_T
Cuts at: 9836 9836
Size: 9841
BlpI GC'TnA_GC
Cuts at: 4341 7258 4341
Size: 2917 6924
Bme1580I G_kGCm'C
Cuts at: 1790 2207 2388 2451 2487 3039 3115 3275 3708
Size: 417 181 63 36 552 76 160 433
Cuts at: 3708 3800 3863 3950 4059 4158 4302 4560 4929
Size: 92 63 87 109 99 144 258 369
Cuts at: 4929 5227 5336 5842 5894 6003 6048 6148 6250
Size: 298 109 506 52 109 45 100 102
Cuts at: 6250 6267 6275 6321 6412 6518 7902 9058 1790
Size: 17 8 46 91 106 1384 1156 2573
Fragments arranged by size:
2573 1384 1156 552 506 433 417 369
298 258 181 160 144 109 109 109
106 102 100 99 92 91 87 76
63 63 52 46 45 36 17 8
BmgBI CAC'GTC
Cuts at: 1318 2183 2317 1318
Size: 865 134 8842
Fragments arranged by size:
8842 865 134
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3881 5484 7572
Size: 416 53 83 399 24 2464 1603 2088
Cuts at: 7572 8530 442
Size: 958 1753
Fragments arranged by size:
2464 2088 1753 1603 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2583 2625 3288 3429 3786 3831 3930 4344
Size: 1344 42 663 141 357 45 99 414
Cuts at: 4344 4500 5442 5466 5745 5943 6288 6559 8501
Size: 156 942 24 279 198 345 271 1942
Cuts at: 8501 1239
Size: 2579
Fragments arranged by size:
2579 1942 1344 942 663 414 357 345
279 271 198 156 141 99 45 42
24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2950 3581 7067 7966 8831 9129 2950
Size: 631 3486 899 865 298 3662
Fragments arranged by size:
3662 3486 899 865 631 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2007 2641 3021 3141 3237 3552 4661
Size: 617 508 634 380 120 96 315 1109
Cuts at: 4661 4813 5649 5742 6032 6120 8504 882
Size: 152 836 93 290 88 2384 2219
Fragments arranged by size:
2384 2219 1109 836 634 617 508 380
315 290 152 120 96 93 88
BsaAI yAC'GTr
Cuts at: 493 1204 7632 493
Size: 711 6428 2702
Fragments arranged by size:
6428 2702 711
BsaBI GATnn'nnATC
Cuts at: 2982 3192 9835 2982
Size: 210 6643 2988
Fragments arranged by size:
6643 2988 210
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3693 3915 5858 7648
Size: 53 83 186 936 2160 222 1943 1790
Cuts at: 7648 8030 8665 275
Size: 382 635 1451
Fragments arranged by size:
2160 1943 1790 1451 936 635 382 222
186 83 53
BsaWI w'CCGG_w
Cuts at: 8274 8672 8946 9093 9504 8274
Size: 398 274 147 411 8611
Fragments arranged by size:
8611 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2461 2491 3561 3591 4615
Size: 30 165 30 1058 30 1070 30 1024
Cuts at: 4615 4645 5464 5494 6497 6527 1178
Size: 30 819 30 1003 30 4492
Fragments arranged by size:
4492 1070 1058 1024 1003 819 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 3214 4686 1186
Size: 1363 665 1472 6341
Fragments arranged by size:
6341 1472 1363 665
BseYI C'CCAG_C
Cuts at: 2059 3443 5500 6314 6841 6845 9044 9540 2059
Size: 1384 2057 814 527 4 2199 496 2360
Fragments arranged by size:
2360 2199 2057 1384 814 527 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4257 1163
Size: 573 2521 6747
Fragments arranged by size:
6747 2521 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2602 7291 8052 9080 1540
Size: 275 186 601 4689 761 1028 2301
Fragments arranged by size:
4689 2301 1028 761 601 275 186
BsiHKAI G_wGCw'C
Cuts at: 2641 3625 4342 4543 5554 5761 5959 6412 7902
Size: 984 717 201 1011 207 198 453 1490
Cuts at: 7902 7987 9058 2641
Size: 85 1071 3424
Fragments arranged by size:
3424 1490 1071 1011 984 717 453 207
201 198 85
BsmI GAATG_Cn'
Cuts at: 3455 4743 5046 6980 7352 7445 3455
Size: 1288 303 1934 372 93 5851
Fragments arranged by size:
5851 1934 1288 372 303 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3906 4167 6894 3906
Size: 261 2727 6853
Fragments arranged by size:
6853 2727 261
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2207 2290 2388 2451 2487 2641 3039
Size: 399 417 83 98 63 36 154 398
Cuts at: 3039 3115 3275 3321 3415 3597 3625 3708 3750
Size: 76 160 46 94 182 28 83 42
Cuts at: 3750 3800 3863 3950 4059 4158 4302 4342 4543
Size: 50 63 87 109 99 144 40 201
Cuts at: 4543 4560 4929 5227 5336 5554 5761 5789 5842
Size: 17 369 298 109 218 207 28 53
Cuts at: 5842 5894 5959 6003 6048 6148 6166 6250 6267
Size: 52 65 44 45 100 18 84 17
Cuts at: 6267 6275 6304 6321 6412 6431 6510 6518 6611
Size: 8 29 17 91 19 79 8 93
Cuts at: 6611 7064 7160 7902 7987 9058 1391
Size: 453 96 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 417 399 398 369
298 218 207 201 182 160 154 144
109 109 100 99 98 96 94 93
91 87 85 84 83 83 79 76
65 63 63 53 52 50 46 45
44 42 40 36 29 28 28 19
18 17 17 17 8 8
BspEI T'CCGG_A
Cuts at: 9504 9504
Size: 9841
BspHI T'CATG_A
Cuts at: 7730 9460 7730
Size: 1730 8111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3058 4169 7004 7196 844
Size: 2214 1111 2835 192 3489
Fragments arranged by size:
3489 2835 2214 1111 192
BsrBI CCG'CTC
Cuts at: 1394 1823 3679 5329 7728 1394
Size: 429 1856 1650 2399 3507
Fragments arranged by size:
3507 2399 1856 1650 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6985 8336 8510 65
Size: 1183 5737 1351 174 1396
Fragments arranged by size:
5737 1396 1351 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 3917 7088 8485 8672 1700
Size: 110 220 1887 3171 1397 187 2869
Fragments arranged by size:
3171 2869 1887 1397 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 3458 96
Size: 3362 6479
BssHII G'CGCG_C
Cuts at: 1925 2020 1925
Size: 95 9746
BssSI C'ACGA_G
Cuts at: 7901 8913 7901
Size: 1012 8829
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3083 794
Size: 2289 7552
BstBI TT'CG_AA
Cuts at: 8645 8645
Size: 9841
BstEII G'GTnAC_C
Cuts at: 1184 2918 3092 5690 1184
Size: 1734 174 2598 5335
Fragments arranged by size:
5335 2598 1734 174
BstXI CCAn_nnnn'nTGG
Cuts at: 3429 6849 3429
Size: 3420 6421
BstYI r'GATC_y
Cuts at: 1972 4226 4262 5586 5630 7171 7538 7923 7940
Size: 2254 36 1324 44 1541 367 385 17
Cuts at: 7940 9392 9478 9490 9836 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2254 1977 1541 1452 1324 385 367 346
86 44 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 3415 4352 5581 5590 5650
Size: 552 939 69 1342 937 1229 9 60
Cuts at: 5650 6735 6988 513
Size: 1085 253 3366
Fragments arranged by size:
3366 1342 1229 1085 939 937 552 253
69 60 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2773 3756 6781 6787 487
Size: 683 56 1547 983 3025 6 3541
Fragments arranged by size:
3541 3025 1547 983 683 56 6
BtsI GCAGTG_nn'
Cuts at: 2152 2522 2848 4245 6099 6711 6835 7357 8151
Size: 370 326 1397 1854 612 124 522 794
Cuts at: 8151 8171 2152
Size: 20 3822
Fragments arranged by size:
3822 1854 1397 794 612 522 370 326
124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9841
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9806
DraI TTT'AAA
Cuts at: 4723 4903 7145 4723
Size: 180 2242 7419
Fragments arranged by size:
7419 2242 180
DraIII CAC_nnn'GTG
Cuts at: 5846 5846
Size: 9841
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2625 5123 8848 809
Size: 507 1309 2498 3725 1802
Fragments arranged by size:
3725 2498 1802 1309 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2599 3565
Size: 54 1425 50 275 18 198 571 966
Cuts at: 3565 4492 4546 5092 5543 6397 6738 6792 6913
Size: 927 54 546 451 854 341 54 121
Cuts at: 6913 7288 8177 9806 8
Size: 375 889 1629 43
Fragments arranged by size:
1629 1425 966 927 889 854 571 546
451 375 341 275 198 121 54 54
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2599 7288 1537
Size: 275 787 4689 4090
Fragments arranged by size:
4689 4090 787 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2131 3370 5925 6322 7771 1353
Size: 778 1239 2555 397 1449 3423
Fragments arranged by size:
3423 2555 1449 1239 778 397
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5521 6868 7060 8428 8802 8948
Size: 1064 138 3932 1347 192 1368 374 146
Cuts at: 8948 387
Size: 1280
Fragments arranged by size:
3932 1368 1347 1280 1064 374 192 146
138
EcoICRI GAG'CTC
Cuts at: 2639 4340 4541 5552 5759 5957 2639
Size: 1701 201 1011 207 198 6523
Fragments arranged by size:
6523 1701 1011 207 201 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2583 2625 3288 3429 3786 3831 3930
Size: 393 951 42 663 141 357 45 99
Cuts at: 3930 4344 4481 4500 4895 5339 5375 5442 5466
Size: 414 137 19 395 444 36 67 24
Cuts at: 5466 5745 5780 5943 6288 6559 7904 8501 9288
Size: 279 35 163 345 271 1345 597 787
Cuts at: 9288 9556 1239
Size: 268 1524
Fragments arranged by size:
1524 1345 951 787 663 597 444 414
395 393 357 345 279 271 268 163
141 137 99 67 45 42 36 35
24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2172 2172
Size: 9841
EcoO109I rG'GnC_Cy
Cuts at: 2204 2303 2447 2448 2484 2591 2750 2990 3008
Size: 99 144 1 36 107 159 240 18
Cuts at: 3008 3035 3036 3111 3164 3224 3271 3299 3308
Size: 27 1 75 53 60 47 28 9
Cuts at: 3308 3503 3773 3796 3797 3808 3860 3947 3966
Size: 195 270 23 1 11 52 87 19
Cuts at: 3966 4055 4082 4091 4154 4199 4217 4271 4299
Size: 89 27 9 63 45 18 54 28
Cuts at: 4299 4315 4412 4484 4556 4557 4612 4746 4918
Size: 16 97 72 72 1 55 134 172
Cuts at: 4918 4926 4994 5432 5672 5720 5890 6000 6044
Size: 8 68 438 240 48 170 110 44
Cuts at: 6044 6107 6116 6218 6236 6263 6264 6271 6272
Size: 63 9 102 18 27 1 7 1
Cuts at: 6272 6318 6371 6592 6787 2204
Size: 46 53 221 195 5258
Fragments arranged by size:
5258 438 270 240 240 221 195 195
172 170 159 144 134 110 107 102
99 97 89 87 75 72 72 68
63 63 60 55 54 53 53 52
48 47 46 45 44 36 28 28
27 27 27 23 19 18 18 18
16 11 9 9 9 8 7 1
1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2358 7150 7528 2358
Size: 4792 378 4671
Fragments arranged by size:
4792 4671 378
EcoRV GAT'ATC
Cuts at: 2549 2549
Size: 9841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5380 7039 5380
Size: 1659 8182
FseI GG_CCGG'CC
Cuts at: 2034 7092 2034
Size: 5058 4783
FspI TGC'GCA
Cuts at: 8347 8347
Size: 9841
HaeII r_GCGC'y
Cuts at: 1678 4632 5861 8988 1678
Size: 2954 1229 3127 2531
Fragments arranged by size:
3127 2954 2531 1229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4757 4789 5464 5496 6202
Size: 32 135 32 3185 32 675 32 706
Cuts at: 6202 6234 8482 8514 8556 8588 1373
Size: 32 2248 32 42 32 2626
Fragments arranged by size:
3185 2626 2248 706 675 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7185 7431 669
Size: 988 281 5247 246 3079
Fragments arranged by size:
5247 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9841
HpaI GTT'AAC
Cuts at: 7431 7431
Size: 9841
KasI G'GCGC_C
Cuts at: 5857 5857
Size: 9841
KpnI G_GTAC'C
Cuts at: 7166 7166
Size: 9841
MfeI C'AATT_G
Cuts at: 7440 7440
Size: 9841
MluI A'CGCG_T
Cuts at: 9717 9717
Size: 9841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2614 3991 5850 6226 8955 9139 1305
Size: 1309 1377 1859 376 2729 184 2007
Fragments arranged by size:
2729 2007 1859 1377 1309 376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4548 6399 6915 10
Size: 54 1425 343 2716 1851 516 2936
Fragments arranged by size:
2936 2716 1851 1425 516 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3995 7799 8158 8317 518
Size: 648 2829 3804 359 159 2042
Fragments arranged by size:
3804 2829 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3417 5592 6721 6771 6845 6939 6990 7116
Size: 1811 2175 1129 50 74 94 51 126
Cuts at: 7116 7257 7934 9082 9327 1606
Size: 141 677 1148 245 2120
Fragments arranged by size:
2175 2120 1811 1148 1129 677 245 141
126 94 74 51 50
NaeI GCC'GGC
Cuts at: 2032 3919 7090 2032
Size: 1887 3171 4783
Fragments arranged by size:
4783 3171 1887
NarI GG'CG_CC
Cuts at: 5858 5858
Size: 9841
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8350
Fragments arranged by size:
8350 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9841
NgoMIV G'CCGG_C
Cuts at: 2030 3917 7088 2030
Size: 1887 3171 4783
Fragments arranged by size:
4783 3171 1887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9841
NotI GC'GGCC_GC
Cuts at: 7288 7288
Size: 9841
NspI r_CATG'y
Cuts at: 1132 1229 2615 4804 7199 8744 1132
Size: 97 1386 2189 2395 1545 2229
Fragments arranged by size:
2395 2229 2189 1545 1386 97
PasI CC'CwG_GG
Cuts at: 1348 2209 2388 2425 2606 3032 3107 3389 3581
Size: 861 179 37 181 426 75 282 192
Cuts at: 3581 3590 4158 4205 4304 4616 4922 5054 5072
Size: 9 568 47 99 312 306 132 18
Cuts at: 5072 5156 5228 5402 5969 6004 6048 6223 6242
Size: 84 72 174 567 35 44 175 19
Cuts at: 6242 6268 1348
Size: 26 4921
Fragments arranged by size:
4921 861 568 567 426 312 306 282
192 181 179 175 174 132 99 84
75 72 47 44 37 35 26 19
18 9
PciI A'CATG_T
Cuts at: 8740 8740
Size: 9841
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5982 6595 6922 1108
Size: 4874 613 327 4027
Fragments arranged by size:
4874 4027 613 327
PfoI T'CCnGG_A
Cuts at: 2362 2905 3304 3490 3625 3820 3847 4399 4516
Size: 543 399 186 135 195 27 552 117
Cuts at: 4516 4705 5437 5572 5626 5923 5959 2362
Size: 189 732 135 54 297 36 6244
Fragments arranged by size:
6244 732 552 543 399 297 195 189
186 135 135 117 54 36 27
PmeI GTTT'AAAC
Cuts at: 7145 7145
Size: 9841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7910 7942 9423 9455 7910
Size: 32 1481 32 8296
Fragments arranged by size:
8296 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2303 2591 2750 3008 3164 3224 3308 3503 3808
Size: 288 159 258 156 60 84 195 305
Cuts at: 3808 3966 4082 4199 4217 4271 4484 4612 4746
Size: 158 116 117 18 54 213 128 134
Cuts at: 4746 4918 6116 6218 6371 6592 2303
Size: 172 1198 102 153 221 5552
Fragments arranged by size:
5552 1198 305 288 258 221 213 195
172 159 158 156 153 134 128 117
116 102 84 60 54 18
PshAI GACnn'nnGTC
Cuts at: 1613 2436 4179 5423 1613
Size: 823 1743 1244 6031
Fragments arranged by size:
6031 1743 1244 823
PsiI TTA'TAA
Cuts at: 7411 7411
Size: 9841
PspOMI G'GGCC_C
Cuts at: 2203 2384 2447 2483 3035 3111 3271 3796 3859
Size: 181 63 36 552 76 160 525 63
Cuts at: 3859 3946 4055 4154 4298 4556 4925 5890 5999
Size: 87 109 99 144 258 369 965 109
Cuts at: 5999 6044 6263 6271 6317 2203
Size: 45 219 8 46 5727
Fragments arranged by size:
5727 965 552 525 369 258 219 181
160 144 109 109 99 87 76 63
63 46 45 36 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3826 3858 3826
Size: 32 9809
PstI C_TGCA'G
Cuts at: 830 2256 7193 830
Size: 1426 4937 3478
Fragments arranged by size:
4937 3478 1426
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9841
PvuII CAG'CTG
Cuts at: 6845 6939 6845
Size: 94 9747
SacI G_AGCT'C
Cuts at: 2641 4342 4543 5554 5761 5959 2641
Size: 1701 201 1011 207 198 6523
Fragments arranged by size:
6523 1701 1011 207 201 198
SacII CC_GC'GG
Cuts at: 3418 5593 6991 3418
Size: 2175 1398 6268
Fragments arranged by size:
6268 2175 1398
SalI G'TCGA_C
Cuts at: 1936 7183 1936
Size: 5247 4594
SanDI GG'GwC_CC
Cuts at: 2750 3008 3224 3808 3966 4612 4746 4918 6116
Size: 258 216 584 158 646 134 172 1198
Cuts at: 6116 6218 2750
Size: 102 6373
Fragments arranged by size:
6373 1198 646 584 258 216 172 158
134 102
SapI GCTCTTCn'nnn_
Cuts at: 3370 5925 3370
Size: 2555 7286
SbfI CC_TGCA'GG
Cuts at: 7193 7193
Size: 9841
ScaI AGT'ACT
Cuts at: 8089 9801 8089
Size: 1712 8129
SexAI A'CCwGG_T
Cuts at: 5347 5563 5842 5905 6286 7137 5347
Size: 216 279 63 381 851 8051
Fragments arranged by size:
8051 851 381 279 216 63
SfcI C'TryA_G
Cuts at: 826 1045 2252 7189 8324 9005 9196 826
Size: 219 1207 4937 1135 681 191 1471
Fragments arranged by size:
4937 1471 1207 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2482 3573 3771 3882 3891 3954 4251 4297 4473
Size: 1091 198 111 9 63 297 46 176
Cuts at: 4473 4500 2482
Size: 27 7823
Fragments arranged by size:
7823 1091 297 198 176 111 63 46
27 9
SfoI GGC'GCC
Cuts at: 5859 5859
Size: 9841
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9841
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9841
SmaI CCC'GGG
Cuts at: 1872 2247 2373 2482 3005 3099 3153 3316 3709
Size: 375 126 109 523 94 54 163 393
Cuts at: 3709 4348 4753 4825 6151 6169 6432 7168 1872
Size: 639 405 72 1326 18 263 736 4545
Fragments arranged by size:
4545 1326 736 639 523 405 393 375
263 163 126 109 94 72 54 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2929 3596 7082 7945 8846 9108
Size: 197 926 986 667 3486 863 901 262
Cuts at: 9108 820
Size: 1553
Fragments arranged by size:
3486 1553 986 926 901 863 667 262
197
SnaBI TAC'GTA
Cuts at: 493 7632 493
Size: 7139 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9841
SphI G_CATG'C
Cuts at: 1132 4804 7199 1132
Size: 3672 2395 3774
Fragments arranged by size:
3774 3672 2395
SrfI GCCC'GGGC
Cuts at: 4348 6432 4348
Size: 2084 7757
SspI AAT'ATT
Cuts at: 5 52 7765 5
Size: 47 7713 2081
Fragments arranged by size:
7713 2081 47
StuI AGG'CCT
Cuts at: 1517 2973 3207 4074 4110 4395 4476 4503 5241
Size: 1456 234 867 36 285 81 27 738
Cuts at: 5241 5316 6013 1517
Size: 75 697 5345
Fragments arranged by size:
5345 1456 867 738 697 285 234 81
75 36 27
StyI C'CwwG_G
Cuts at: 513 1065 2004 2307 2488 3012 3115 3228 3507
Size: 552 939 303 181 524 103 113 279
Cuts at: 3507 4059 4435 4560 4795 5035 5428 5616 5752
Size: 552 376 125 235 240 393 188 136
Cuts at: 5752 6111 6120 6322 7280 513
Size: 359 9 202 958 3074
Fragments arranged by size:
3074 958 939 552 552 524 393 376
359 303 279 240 235 202 188 181
136 125 113 103 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2829 6837 7868 7885 8038 8223 8668
Size: 1255 1249 4008 1031 17 153 185 445
Cuts at: 8668 325
Size: 1498
Fragments arranged by size:
4008 1498 1255 1249 1031 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 3458 6558
Size: 274 80 33 51 1443 846 635 3100
Cuts at: 6558 6645 7017 8087 9799 96
Size: 87 372 1070 1712 138
Fragments arranged by size:
3100 1712 1443 1070 846 635 372 274
138 87 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2461 2493 3703 3735 3924 3956 4615 4647 6497
Size: 32 1210 32 189 32 659 32 1850
Cuts at: 6497 6529 9801 9833 2461
Size: 32 3272 32 2469
Fragments arranged by size:
3272 2469 1850 1210 659 189 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2184 1316
Size: 868 8973
XbaI T'CTAG_A
Cuts at: 6943 7177 6943
Size: 234 9607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2216 2314 4237 4442 4802 5435 6011 6091 6329
Size: 98 1923 205 360 633 576 80 238
Cuts at: 6329 6392 6517 6733 2216
Size: 63 125 216 5324
Fragments arranged by size:
5324 1923 633 576 360 238 216 205
125 98 80 63
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9841
XmaI C'CCGG_G
Cuts at: 1870 2245 2371 2480 3003 3097 3151 3314 3707
Size: 375 126 109 523 94 54 163 393
Cuts at: 3707 4346 4751 4823 6149 6167 6430 7166 1870
Size: 639 405 72 1326 18 263 736 4545
Fragments arranged by size:
4545 1326 736 639 523 405 393 375
263 163 126 109 94 72 54 18
XmnI GAAnn'nnTTC
Cuts at: 5360 7970 5360
Size: 2610 7231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7649 276
Size: 53 83 186 936 6115 2468
Fragments arranged by size:
6115 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I FspAI NruI NsiI PacI PmlI
PspXI RsrII SwaI