Restriction map for
pFN21AB9017
(Circular) (Six-base) MAPSORT of: pFN21AB9017.seq Check: 6893 from: 1 to: 6034
pFN21AB9017 6034 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3079 3377 1937
Size: 1142 298 4594
Fragments arranged by size:
4594 1142 298
Acc65I G'GTAC_C
Cuts at: 3355 3355
Size: 6034
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2184 2633 4097 5481 5749 1632
Size: 552 449 1464 1384 268 1917
Fragments arranged by size:
1917 1464 1384 552 449 268
AflII C'TTAA_G
Cuts at: 820 1017 3235 820
Size: 197 2218 3619
Fragments arranged by size:
3619 2218 197
AflIII A'CryG_T
Cuts at: 1203 4933 5910 1203
Size: 3730 977 1327
Fragments arranged by size:
3730 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6034
AlwNI CAG_nnn'CTG
Cuts at: 2752 3777 5349 2752
Size: 1025 1572 3437
Fragments arranged by size:
3437 1572 1025
ApaLI G'TGCA_C
Cuts at: 2311 3087 4091 5247 2311
Size: 776 1004 1156 3098
Fragments arranged by size:
3098 1156 1004 776
ApoI r'AATT_y
Cuts at: 1493 2537 2807 2831 3070 3343 3569 3721 5830
Size: 1044 270 24 239 273 226 152 2109
Cuts at: 5830 1493
Size: 1697
Fragments arranged by size:
2109 1697 1044 273 270 239 226 152
24
AseI AT'TA_AT
Cuts at: 160 1032 2693 2717 4588 160
Size: 872 1661 24 1871 1606
Fragments arranged by size:
1871 1661 1606 872 24
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2490 2517 3359 1870
Size: 73 202 345 27 842 4545
Fragments arranged by size:
4545 842 345 202 73 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2714 2747 2714
Size: 33 6001
BamHI G'GATC_C
Cuts at: 3364 3731 3364
Size: 367 5667
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3355 4810 618
Size: 325 217 627 1568 1455 1842
Fragments arranged by size:
1842 1568 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3153 3353 1391
Size: 1762 200 4072
Fragments arranged by size:
4072 1762 200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2933 928
Size: 971 1034 4029
Fragments arranged by size:
4029 1034 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4225 4259 1903
Size: 34 2288 34 3678
Fragments arranged by size:
3678 2288 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2742 3311 3926 5136 2742
Size: 569 615 1210 3640
Fragments arranged by size:
3640 1210 615 569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6034
BfrBI ATG'CAT
Cuts at: 2825 2825
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3117 4645 136
Size: 107 122 71 631 605 1445 1528 1525
Fragments arranged by size:
1528 1525 1445 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6029 6029
Size: 6034
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2315 3091 4095 5251 1790
Size: 525 776 1004 1156 2573
Fragments arranged by size:
2573 1156 1004 776 525
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3765 4723 442
Size: 416 53 83 399 24 2348 958 1753
Fragments arranged by size:
2348 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2960 3198 4694 1239
Size: 1721 238 1496 2579
Fragments arranged by size:
2579 1721 1496 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2220 2584 2906 4159 5024 5322 2220
Size: 364 322 1253 865 298 2932
Fragments arranged by size:
2932 1253 865 364 322 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2676 4697 882
Size: 617 1177 2021 2219
Fragments arranged by size:
2219 2021 1177 617
BsaAI yAC'GTr
Cuts at: 493 1204 3825 493
Size: 711 2621 2702
Fragments arranged by size:
2702 2621 711
BsaBI GATnn'nnATC
Cuts at: 2634 6028 2634
Size: 3394 2640
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3841 4223 4858 275
Size: 53 83 186 936 2308 382 635 1451
Fragments arranged by size:
2308 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4467 4865 5139 5286 5697 4467
Size: 398 274 147 411 4804
Fragments arranged by size:
4804 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5809
Fragments arranged by size:
5809 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2056 2342 1186
Size: 870 286 4878
Fragments arranged by size:
4878 870 286
BseYI C'CCAG_C
Cuts at: 2222 5237 5733 2222
Size: 3015 496 2523
Fragments arranged by size:
3015 2523 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5461
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3484 4245 5273 1540
Size: 275 186 1483 761 1028 2301
Fragments arranged by size:
2301 1483 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2315 3091 3153 3259 4095 4180 5251 2315
Size: 776 62 106 836 85 1071 3098
Fragments arranged by size:
3098 1071 836 776 106 85 62
BsmI GAATG_Cn'
Cuts at: 3545 3638 3545
Size: 93 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2315 3091 3153 3259 3353 4095 4180
Size: 399 525 776 62 106 94 742 85
Cuts at: 4180 5251 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 776 742 525 399 106 94
85 62
BspEI T'CCGG_A
Cuts at: 5697 5697
Size: 6034
BspHI T'CATG_A
Cuts at: 3923 5653 3923
Size: 1730 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3389 844
Size: 2545 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3921 1394
Size: 429 2098 3507
Fragments arranged by size:
3507 2098 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2035 4529 4703 65
Size: 1183 787 2494 174 1396
Fragments arranged by size:
2494 1396 1183 787 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3018 4678 4865 1700
Size: 110 1208 1660 187 2869
Fragments arranged by size:
2869 1660 1208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6034
BssSI C'ACGA_G
Cuts at: 4094 5106 4094
Size: 1012 5022
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2420 794
Size: 1626 4408
BstBI TT'CG_AA
Cuts at: 3068 4838 3068
Size: 1770 4264
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6034
BstXI CCAn_nnnn'nTGG
Cuts at: 3061 3061
Size: 6034
BstYI r'GATC_y
Cuts at: 1972 3364 3731 4116 4133 5585 5671 5683 6029
Size: 1392 367 385 17 1452 86 12 346
Cuts at: 6029 1972
Size: 1977
Fragments arranged by size:
1977 1452 1392 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2686 513
Size: 552 939 682 3861
Fragments arranged by size:
3861 939 682 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 2141 3014 3550 4344 4364 2141
Size: 873 536 794 20 3811
Fragments arranged by size:
3811 873 794 536 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2766 2801 529
Size: 35 2202 35 3762
Fragments arranged by size:
3762 2202 35 35
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2499 3481 4370
Size: 54 1425 50 275 18 669 982 889
Cuts at: 4370 5999 8
Size: 1629 43
Fragments arranged by size:
1629 1425 982 889 669 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3481 1537
Size: 275 1669 4090
Fragments arranged by size:
4090 1669 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2934 3964 1353
Size: 1581 1030 3423
Fragments arranged by size:
3423 1581 1030
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3005 4621 4995 5141 387
Size: 1064 138 1416 1616 374 146 1280
Fragments arranged by size:
1616 1416 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3151 3151
Size: 6034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2184 2633 2960 3198 4097 4694 5481
Size: 393 552 449 327 238 899 597 787
Cuts at: 5481 5749 1239
Size: 268 1524
Fragments arranged by size:
1524 899 787 597 552 449 393 327
268 238
EcoNI CCTnn'n_nnAGG
Cuts at: 2055 2055
Size: 6034
EcoO109I rG'GnC_Cy
Cuts at: 2749 2749
Size: 6034
EcoRI G'AATT_C
Cuts at: 3070 3343 3721 3070
Size: 273 378 5383
Fragments arranged by size:
5383 378 273
EcoRV GAT'ATC
Cuts at: 2632 2632
Size: 6034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2963 2963
Size: 6034
FspI TGC'GCA
Cuts at: 4540 4540
Size: 6034
HaeII r_GCGC'y
Cuts at: 1678 2947 5181 1678
Size: 1269 2234 2531
Fragments arranged by size:
2531 2234 1269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3246 3278 4675 4707 4749
Size: 32 135 32 1674 32 1397 32 42
Cuts at: 4749 4781 1373
Size: 32 2626
Fragments arranged by size:
2626 1674 1397 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3378 3624 669
Size: 988 281 1440 246 3079
Fragments arranged by size:
3079 1440 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6034
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KpnI G_GTAC'C
Cuts at: 3359 3359
Size: 6034
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5148 5332 1305
Size: 3843 184 2007
Fragments arranged by size:
3843 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4212
Fragments arranged by size:
4212 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2184 3059 3992 4351 4510 518
Size: 648 1018 875 933 359 159 2042
Fragments arranged by size:
2042 1018 933 875 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2226 3450 4127 5275 5520 1606
Size: 620 1224 677 1148 245 2120
Fragments arranged by size:
2120 1224 1148 677 620 245
NaeI GCC'GGC
Cuts at: 3020 3020
Size: 6034
NcoI C'CATG_G
Cuts at: 513 1065 2004 2686 513
Size: 552 939 682 3861
Fragments arranged by size:
3861 939 682 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NgoMIV G'CCGG_C
Cuts at: 3018 3018
Size: 6034
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NsiI A_TGCA'T
Cuts at: 2827 2827
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 2512 2609 3392 4937 1132
Size: 97 1283 97 783 1545 2229
Fragments arranged by size:
2229 1545 1283 783 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6034
PciI A'CATG_T
Cuts at: 4933 4933
Size: 6034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2827 1108
Size: 1719 4315
PfoI T'CCnGG_A
Cuts at: 2745 2985 2745
Size: 240 5794
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 5616 5648 4103
Size: 32 1481 32 4489
Fragments arranged by size:
4489 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2749 2749
Size: 6034
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6034
PsiI TTA'TAA
Cuts at: 2678 3604 2678
Size: 926 5108
PspXI vC'TCGA_Gb
Cuts at: 2145 2517 2145
Size: 372 5662
PstI C_TGCA'G
Cuts at: 830 3386 830
Size: 2556 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
PvuII CAG'CTG
Cuts at: 2226 2226
Size: 6034
SacI G_AGCT'C
Cuts at: 3153 3153
Size: 6034
SalI G'TCGA_C
Cuts at: 1936 3376 1936
Size: 1440 4594
SapI GCTCTTCn'nnn_
Cuts at: 2934 2934
Size: 6034
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SfcI C'TryA_G
Cuts at: 826 1045 2448 3382 4517 5198 5389 826
Size: 219 1403 934 1135 681 191 1471
Fragments arranged by size:
1471 1403 1135 934 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 3361 1872
Size: 1489 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2145 2235 2517 2563 2921 3235
Size: 197 926 202 90 282 46 358 314
Cuts at: 3235 4138 5039 5301 820
Size: 903 901 262 1553
Fragments arranged by size:
1553 926 903 901 358 314 282 262
202 197 90 46
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 3392 1132
Size: 2260 3774
SspI AAT'ATT
Cuts at: 5 52 3958 5
Size: 47 3906 2081
Fragments arranged by size:
3906 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6034
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 2686 2736 3473 513
Size: 552 939 318 364 50 737 3074
Fragments arranged by size:
3074 939 737 552 364 318 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4061 4078 4231 4416 4861 325
Size: 1255 2481 17 153 185 445 1498
Fragments arranged by size:
2481 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 4280 5992
Size: 274 80 33 51 1443 180 2123 1712
Cuts at: 5992 96
Size: 138
Fragments arranged by size:
2123 1712 1443 274 180 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2419 2451 5994 6026 2419
Size: 32 3543 32 2427
Fragments arranged by size:
3543 2427 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6034
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6034
XhoI C'TCGA_G
Cuts at: 1943 2145 2517 1943
Size: 202 372 5460
Fragments arranged by size:
5460 372 202
XmaI C'CCGG_G
Cuts at: 1870 3359 1870
Size: 1489 4545
XmnI GAAnn'nnTTC
Cuts at: 2354 4163 2354
Size: 1809 4225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FseI
FspAI KasI NarI NruI PacI PmlI PspOMI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI