Restriction map for
pFN21AB8999
(Circular) (Six-base) MAPSORT of: pFN21AB8999.seq Check: 1334 from: 1 to: 5776
pFN21AB8999 5776 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3839 5223 5491 1632
Size: 2207 1384 268 1917
Fragments arranged by size:
2207 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2823 3073 4675 5652 1203
Size: 1620 250 1602 977 1327
Fragments arranged by size:
1620 1602 1327 977 250
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 2012
Size: 32 5744
AlwNI CAG_nnn'CTG
Cuts at: 2391 3519 5091 2391
Size: 1128 1572 3076
Fragments arranged by size:
3076 1572 1128
ApaLI G'TGCA_C
Cuts at: 2300 3833 4989 2300
Size: 1533 1156 3087
Fragments arranged by size:
3087 1533 1156
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 1870
Size: 73 1158 4545
Fragments arranged by size:
4545 1158 73
BamHI G'GATC_C
Cuts at: 2898 3106 3473 2898
Size: 208 367 5201
Fragments arranged by size:
5201 367 208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2316 2624 3097 4552 618
Size: 325 217 627 529 308 473 1455 1842
Fragments arranged by size:
1842 1455 627 529 473 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2702 2922 3095 1391
Size: 1311 220 173 4072
Fragments arranged by size:
4072 1311 220 173
BbeI G_GCGC'C
Cuts at: 2320 2628 2320
Size: 308 5468
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 2714 928
Size: 971 211 604 3990
Fragments arranged by size:
3990 971 604 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2315 2349 3967 4001 1903
Size: 34 378 34 1618 34 3678
Fragments arranged by size:
3678 1618 378 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4387 136
Size: 107 122 71 631 605 2715 1525
Fragments arranged by size:
2715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2670 5771 2670
Size: 3101 2675
BlpI GC'TnA_GC
Cuts at: 2291 3193 2291
Size: 902 4874
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2304 2506 3837 4993 1790
Size: 439 75 202 1331 1156 2573
Fragments arranged by size:
2573 1331 1156 439 202 75
BmgBI CAC'GTC
Cuts at: 1318 2368 1318
Size: 1050 4726
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2686 4436 1239
Size: 1447 1750 2579
Fragments arranged by size:
2579 1750 1447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 4766 5064 3901
Size: 865 298 4613
Fragments arranged by size:
4613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 4439 882
Size: 617 555 2385 2219
Fragments arranged by size:
2385 2219 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 2826 3567 493
Size: 711 1622 741 2702
Fragments arranged by size:
2702 1622 741 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2317 2625 3583 3965
Size: 53 83 186 936 784 308 958 382
Cuts at: 3965 4600 275
Size: 635 1451
Fragments arranged by size:
1451 958 936 784 635 382 308 186
83 53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 2978 3008 1178
Size: 30 165 30 609 30 936 30 3946
Fragments arranged by size:
3946 936 609 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2588 1186
Size: 1402 4374
BseYI C'CCAG_C
Cuts at: 2096 2143 2544 4979 5475 2096
Size: 47 401 2435 496 2397
Fragments arranged by size:
2435 2397 496 401 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2362 1163
Size: 573 626 4577
Fragments arranged by size:
4577 626 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2655 2892 2922 3837 3922 4993 2304
Size: 351 237 30 915 85 1071 3087
Fragments arranged by size:
3087 1071 915 351 237 85 30
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
BsmBI CGTCTCn'nnnn_
Cuts at: 2260 2479 2546 2725 2260
Size: 219 67 179 5311
Fragments arranged by size:
5311 219 179 67
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2304 2506 2655 2702 2892 2922
Size: 399 439 75 202 149 47 190 30
Cuts at: 2922 3095 3837 3922 4993 1391
Size: 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 202 190 173
149 85 75 47 30
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 2484 3665 5395 2484
Size: 1181 1730 2865
Fragments arranged by size:
2865 1730 1181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2187 2643 3131 844
Size: 1253 90 456 488 3489
Fragments arranged by size:
3489 1253 488 456 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2641 3663 1394
Size: 429 818 1022 3507
Fragments arranged by size:
3507 1022 818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 2303 3836 4848 2303
Size: 1533 1012 3231
Fragments arranged by size:
3231 1533 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2413 2413
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2670 2898 3106 3473 3858 3875 5327 5413
Size: 698 228 208 367 385 17 1452 86
Cuts at: 5413 5425 5771 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 698 385 367 346 228 208
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2247 513
Size: 552 939 243 4042
Fragments arranged by size:
4042 939 552 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DraIII CAC_nnn'GTG
Cuts at: 2224 2224
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2271 2382
Size: 54 1425 50 275 18 420 21 111
Cuts at: 2382 2403 3223 4112 5741 8
Size: 21 820 889 1629 43
Fragments arranged by size:
1629 1425 889 820 420 275 111 54
50 43 21 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2660 3706 1353
Size: 1307 1046 3423
Fragments arranged by size:
3423 1307 1046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2777 4363 4737 4883 387
Size: 1064 138 1188 1586 374 146 1280
Fragments arranged by size:
1586 1280 1188 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2920 2920
Size: 5776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2686 3839 4436 5223 5491 1239
Size: 393 1054 1153 597 787 268 1524
Fragments arranged by size:
1524 1153 1054 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2320 2628 3048 4923 1678
Size: 642 308 420 1875 2531
Fragments arranged by size:
2531 1875 642 420 308
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373
Size: 32 135 32 2845 32 42 32 2626
Fragments arranged by size:
2845 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2316 2624 2316
Size: 308 5468
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2315 2502 3015 4890 5074 1305
Size: 1010 187 513 1875 184 2007
Fragments arranged by size:
2007 1875 1010 513 187 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 3734 4093 4252 518
Size: 648 1713 855 359 159 2042
Fragments arranged by size:
2042 1713 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2290 2322 2513 2533 2848 3192 3869 5017
Size: 684 32 191 20 315 344 677 1148
Cuts at: 5017 5262 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 684 677 344 315 245 191
32 20
NarI GG'CG_CC
Cuts at: 2317 2625 2317
Size: 308 5468
NcoI C'CATG_G
Cuts at: 513 1065 2004 2247 513
Size: 552 939 243 4042
Fragments arranged by size:
4042 939 552 243
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 2391 1108
Size: 1128 155 4493
Fragments arranged by size:
4493 1128 155
PfoI T'CCnGG_A
Cuts at: 2702 2702
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PmlI CAC'GTG
Cuts at: 2826 2826
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2012 2044 3845 3877 5358 5390 2012
Size: 32 1801 32 1481 32 2398
Fragments arranged by size:
2398 1801 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 3128 830
Size: 2298 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
RsrII CG'GwC_CG
Cuts at: 2785 2785
Size: 5776
SacI G_AGCT'C
Cuts at: 2922 2922
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2660 2660
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SexAI A'CCwGG_T
Cuts at: 2235 2235
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 3124 4259 4940 5131 826
Size: 219 2079 1135 681 191 1471
Fragments arranged by size:
2079 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2318 2626 2318
Size: 308 5468
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3880 4781 5043 820
Size: 197 926 1937 901 262 1553
Fragments arranged by size:
1937 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 3215 513
Size: 552 939 243 968 3074
Fragments arranged by size:
3074 968 939 552 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2104 2740 3803 3820 3973 4158 4603
Size: 1255 524 636 1063 17 153 185 445
Cuts at: 4603 325
Size: 1498
Fragments arranged by size:
1498 1255 1063 636 524 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2661 4022 5734
Size: 274 80 33 51 1443 684 1361 1712
Cuts at: 5734 96
Size: 138
Fragments arranged by size:
1712 1443 1361 684 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2294 2326 5736 5768 2294
Size: 32 3410 32 2302
Fragments arranged by size:
3410 2302 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2692 2692
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI PvuII SacII SanDI SfiI SrfI SwaI