Restriction map for
pFN21AB8997
(Circular) (Six-base) MAPSORT of: pFN21AB8997.seq Check: 5714 from: 1 to: 5929
pFN21AB8997 5929 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2485 2485
Size: 5929
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3739 278
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3272 1937
Size: 1335 4594
Acc65I G'GTAC_C
Cuts at: 3250 3250
Size: 5929
AclI AA'CG_TT
Cuts at: 1563 4056 4429 1563
Size: 2493 373 3063
Fragments arranged by size:
3063 2493 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 2938 3992 5376 5644 1632
Size: 623 683 1054 1384 268 1917
Fragments arranged by size:
1917 1384 1054 683 623 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5732
AflIII A'CryG_T
Cuts at: 1203 4828 5805 1203
Size: 3625 977 1327
Fragments arranged by size:
3625 1327 977
AgeI A'CCGG_T
Cuts at: 4760 4760
Size: 5929
AhdI GACnn_n'nnGTC
Cuts at: 4658 4658
Size: 5929
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5929
AlwNI CAG_nnn'CTG
Cuts at: 3082 3672 5244 3082
Size: 590 1572 3767
Fragments arranged by size:
3767 1572 590
ApaI G_GGCC'C
Cuts at: 2510 2803 2510
Size: 293 5636
ApaLI G'TGCA_C
Cuts at: 3986 5142 3986
Size: 1156 4773
ApoI r'AATT_y
Cuts at: 1493 3238 3464 3616 5725 1493
Size: 1745 226 152 2109 1697
Fragments arranged by size:
2109 1745 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4483 160
Size: 872 3451 1606
Fragments arranged by size:
3451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
AvaI C'yCGr_G
Cuts at: 1870 1943 2679 3254 1870
Size: 73 736 575 4545
Fragments arranged by size:
4545 736 575 73
BamHI G'GATC_C
Cuts at: 3259 3626 3259
Size: 367 5562
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2575 2583 2931 3222 3250
Size: 325 217 627 788 8 348 291 28
Cuts at: 3250 4705 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 788 627 348 325 291 217
28 8
BanII G_rGCy'C
Cuts at: 1391 2163 2510 2660 2803 3248 1391
Size: 772 347 150 143 445 4072
Fragments arranged by size:
4072 772 445 347 150 143
BbeI G_GCGC'C
Cuts at: 2935 2935
Size: 5929
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4958
BbvCI CC'TCA_GC
Cuts at: 2435 2435
Size: 5929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2170 2204 4120 4154 1903
Size: 34 233 34 1916 34 3678
Fragments arranged by size:
3678 1916 233 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3116 3821 5031 2331
Size: 785 705 1210 3229
Fragments arranged by size:
3229 1210 785 705
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5929
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2342 2838 4540
Size: 107 122 71 631 605 670 496 1702
Cuts at: 4540 136
Size: 1525
Fragments arranged by size:
1702 1525 670 631 605 496 122 107
71
BglII A'GATC_T
Cuts at: 5924 5924
Size: 5929
BlpI GC'TnA_GC
Cuts at: 3346 3346
Size: 5929
Bme1580I G_kGCm'C
Cuts at: 1790 2510 2586 2803 3129 3990 5146 1790
Size: 720 76 217 326 861 1156 2573
Fragments arranged by size:
2573 1156 861 720 326 217 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 4618 442
Size: 416 53 83 399 24 2243 958 1753
Fragments arranged by size:
2243 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2708 1056
Size: 1652 4277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 4589 1239
Size: 1163 2187 2579
Fragments arranged by size:
2579 2187 1163
Bpu10I CC'TnA_GC
Cuts at: 2143 2325 2435 2886 2143
Size: 182 110 451 5186
Fragments arranged by size:
5186 451 182 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2766 4054 4919 5217 2336
Size: 430 1288 865 298 3048
Fragments arranged by size:
3048 1288 865 430 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4592 882
Size: 617 3093 2219
Fragments arranged by size:
3093 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3720 493
Size: 711 2516 2702
Fragments arranged by size:
2702 2516 711
BsaBI GATnn'nnATC
Cuts at: 5923 5923
Size: 5929
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2137 2863 2932 3736
Size: 53 83 186 936 604 726 69 804
Cuts at: 3736 4118 4753 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 804 726 635 604 382 186
83 69 53
BsaWI w'CCGG_w
Cuts at: 2242 4362 4760 5034 5181 5592 2242
Size: 2120 398 274 147 411 2579
Fragments arranged by size:
2579 2120 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5704
Fragments arranged by size:
5704 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2300 2371 2455 1186
Size: 1111 3 71 84 4660
Fragments arranged by size:
4660 1111 84 71 3
BseYI C'CCAG_C
Cuts at: 2563 2802 5132 5628 2563
Size: 239 2330 496 2864
Fragments arranged by size:
2864 2330 496 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3095 1163
Size: 573 1359 3997
Fragments arranged by size:
3997 1359 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2774 3379 4140 5168 1540
Size: 275 186 773 605 761 1028 2301
Fragments arranged by size:
2301 1028 773 761 605 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 3990 4075 5146 2163
Size: 1827 85 1071 2946
Fragments arranged by size:
2946 1827 1071 85
BsmI GAATG_Cn'
Cuts at: 2028 3440 3533 2028
Size: 1412 93 4424
Fragments arranged by size:
4424 1412 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2084 2084
Size: 5929
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2510 2586 2660 2803 3129 3248
Size: 399 373 347 76 74 143 326 119
Cuts at: 3248 3990 4075 5146 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 347 326 143
119 85 76 74
BspEI T'CCGG_A
Cuts at: 2242 5592 2242
Size: 3350 2579
BspHI T'CATG_A
Cuts at: 3818 5548 3818
Size: 1730 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2485 3284 844
Size: 1641 799 3489
Fragments arranged by size:
3489 1641 799
BsrBI CCG'CTC
Cuts at: 1394 1823 2142 3816 1394
Size: 429 319 1674 3507
Fragments arranged by size:
3507 1674 429 319
BsrDI GCAATG_nn'
Cuts at: 65 1248 4424 4598 65
Size: 1183 3176 174 1396
Fragments arranged by size:
3176 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2265 2928 4573 4760 1700
Size: 110 455 663 1645 187 2869
Fragments arranged by size:
2869 1645 663 455 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5929
BssSI C'ACGA_G
Cuts at: 2608 3989 5001 2608
Size: 1381 1012 3536
Fragments arranged by size:
3536 1381 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5929
BstBI TT'CG_AA
Cuts at: 4733 4733
Size: 5929
BstEII G'GTnAC_C
Cuts at: 1184 2015 2821 1184
Size: 831 806 4292
Fragments arranged by size:
4292 831 806
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 2309
Size: 5929
BstYI r'GATC_y
Cuts at: 1972 2257 2298 3259 3626 4011 4028 5480 5566
Size: 285 41 961 367 385 17 1452 86
Cuts at: 5566 5578 5924 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 961 385 367 346 285 86
41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3016 513
Size: 552 1951 3426
Fragments arranged by size:
3426 1951 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2973 487
Size: 683 56 1747 3443
Fragments arranged by size:
3443 1747 683 56
BtsI GCAGTG_nn'
Cuts at: 3445 4239 4259 3445
Size: 794 20 5115
Fragments arranged by size:
5115 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5929
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5894
DraI TTT'AAA
Cuts at: 3233 3233
Size: 5929
DraIII CAC_nnn'GTG
Cuts at: 2629 2629
Size: 5929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4936 809
Size: 507 3620 1802
Fragments arranged by size:
3620 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 2830 2839
Size: 54 1425 50 275 18 504 496 9
Cuts at: 2839 3376 4265 5894 8
Size: 537 889 1629 43
Fragments arranged by size:
1629 1425 889 537 504 496 275 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3376 1537
Size: 275 1564 4090
Fragments arranged by size:
4090 1564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 2489 3859 1353
Size: 933 203 1370 3423
Fragments arranged by size:
3423 1370 933 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4516 4890 5036 387
Size: 1064 138 2927 374 146 1280
Fragments arranged by size:
2927 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2161 2161
Size: 5929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2402 2938 3992 4589 5376 5644
Size: 393 623 147 536 1054 597 787 268
Cuts at: 5644 1239
Size: 1524
Fragments arranged by size:
1524 1054 787 623 597 536 393 268
147
EcoO109I rG'GnC_Cy
Cuts at: 2506 2507 2506
Size: 1 5928
EcoRI G'AATT_C
Cuts at: 3238 3616 3238
Size: 378 5551
FspI TGC'GCA
Cuts at: 4435 4435
Size: 5929
HaeII r_GCGC'y
Cuts at: 1678 2332 2935 5076 1678
Size: 654 603 2141 2531
Fragments arranged by size:
2531 2141 654 603
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4570 4602 4644 4676 1373
Size: 32 135 32 2998 32 42 32 2626
Fragments arranged by size:
2998 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3273 3519 669
Size: 988 281 1335 246 3079
Fragments arranged by size:
3079 1335 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5929
HpaI GTT'AAC
Cuts at: 3519 3519
Size: 5929
KasI G'GCGC_C
Cuts at: 2931 2931
Size: 5929
KpnI G_GTAC'C
Cuts at: 3254 3254
Size: 5929
MfeI C'AATT_G
Cuts at: 3528 3528
Size: 5929
MluI A'CGCG_T
Cuts at: 5805 5805
Size: 5929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5043 5227 1305
Size: 3738 184 2007
Fragments arranged by size:
3738 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2832 10
Size: 54 1425 343 1000 3107
Fragments arranged by size:
3107 1425 1000 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2625 2714 3149 3887 4246 4405 518
Size: 648 1459 89 435 738 359 159 2042
Fragments arranged by size:
2042 1459 738 648 435 359 159 89
MspA1I CmG'CkG
Cuts at: 1606 2902 3345 4022 5170 5415 1606
Size: 1296 443 677 1148 245 2120
Fragments arranged by size:
2120 1296 1148 677 443 245
NaeI GCC'GGC
Cuts at: 2930 2930
Size: 5929
NarI GG'CG_CC
Cuts at: 2932 2932
Size: 5929
NcoI C'CATG_G
Cuts at: 513 1065 3016 513
Size: 552 1951 3426
Fragments arranged by size:
3426 1951 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5929
NgoMIV G'CCGG_C
Cuts at: 2928 2928
Size: 5929
NheI G'CTAG_C
Cuts at: 1052 2704 1052
Size: 1652 4277
NotI GC'GGCC_GC
Cuts at: 3376 3376
Size: 5929
NspI r_CATG'y
Cuts at: 1132 1229 2254 2759 3287 4832 1132
Size: 97 1025 505 528 1545 2229
Fragments arranged by size:
2229 1545 1025 528 505 97
PasI CC'CwG_GG
Cuts at: 1348 2393 1348
Size: 1045 4884
PciI A'CATG_T
Cuts at: 4828 4828
Size: 5929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2699 1108
Size: 1591 4338
PfoI T'CCnGG_A
Cuts at: 2487 2487
Size: 5929
PmeI GTTT'AAAC
Cuts at: 3233 3233
Size: 5929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 5511 5543 3998
Size: 32 1481 32 4384
Fragments arranged by size:
4384 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3083 1613
Size: 1470 4459
PsiI TTA'TAA
Cuts at: 3499 3499
Size: 5929
PspOMI G'GGCC_C
Cuts at: 2506 2799 2506
Size: 293 5636
PstI C_TGCA'G
Cuts at: 830 2193 2473 3281 830
Size: 1363 280 808 3478
Fragments arranged by size:
3478 1363 808 280
PvuI CG_AT'CG
Cuts at: 2001 2774 2001
Size: 773 5156
SacI G_AGCT'C
Cuts at: 2163 2163
Size: 5929
SalI G'TCGA_C
Cuts at: 1936 3271 1936
Size: 1335 4594
SapI GCTCTTCn'nnn_
Cuts at: 2286 2489 2286
Size: 203 5726
SbfI CC_TGCA'GG
Cuts at: 3281 3281
Size: 5929
ScaI AGT'ACT
Cuts at: 4177 5889 4177
Size: 1712 4217
SfcI C'TryA_G
Cuts at: 826 1045 2189 2469 3277 4412 5093 5284 826
Size: 219 1144 280 808 1135 681 191 1471
Fragments arranged by size:
1471 1144 1135 808 681 280 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2342 2838 2342
Size: 496 5433
SfoI GGC'GCC
Cuts at: 2933 2933
Size: 5929
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5929
SmaI CCC'GGG
Cuts at: 1872 2681 3256 1872
Size: 809 575 4545
Fragments arranged by size:
4545 809 575
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 2781 4033 4934 5196 820
Size: 197 926 372 466 1252 901 262 1553
Fragments arranged by size:
1553 1252 926 901 466 372 262 197
SnaBI TAC'GTA
Cuts at: 493 3720 493
Size: 3227 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5929
SphI G_CATG'C
Cuts at: 1132 3287 1132
Size: 2155 3774
SspI AAT'ATT
Cuts at: 5 52 3853 5
Size: 47 3801 2081
Fragments arranged by size:
3801 2081 47
StuI AGG'CCT
Cuts at: 1517 3005 1517
Size: 1488 4441
StyI C'CwwG_G
Cuts at: 513 1065 3016 3368 513
Size: 552 1951 352 3074
Fragments arranged by size:
3074 1951 552 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3210 3956 3973 4126 4311 4756 325
Size: 1255 1630 746 17 153 185 445 1498
Fragments arranged by size:
1630 1498 1255 746 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4175 5887 96
Size: 274 80 33 51 1443 2198 1712 138
Fragments arranged by size:
2198 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5889 5921 5889
Size: 32 5897
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5929
XbaI T'CTAG_A
Cuts at: 3265 3265
Size: 5929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3157 3157
Size: 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5929
XmaI C'CCGG_G
Cuts at: 1870 2679 3254 1870
Size: 809 575 4545
Fragments arranged by size:
4545 809 575
XmnI GAAnn'nnTTC
Cuts at: 4058 4058
Size: 5929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3737 276
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SrfI SwaI