Restriction map for
pFN21AB8996
(Circular) (Six-base) MAPSORT of: pFN21AB8996.seq Check: 5978 from: 1 to: 5926
pFN21AB8996 5926 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3989 5373 5641 1632
Size: 2357 1384 268 1917
Fragments arranged by size:
2357 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 3062 1166
Size: 1896 4030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 2515 2679 2776 3669 5241 2515
Size: 164 97 893 1572 3200
Fragments arranged by size:
3200 1572 893 164 97
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2146 2398 2469 3251 1870
Size: 73 203 252 71 782 4545
Fragments arranged by size:
4545 782 252 203 73 71
BamHI G'GATC_C
Cuts at: 2141 3256 3623 2141
Size: 1115 367 4444
Fragments arranged by size:
4444 1115 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2402 2785 2862 3247 4702
Size: 325 217 627 615 383 77 385 1455
Cuts at: 4702 618
Size: 1842
Fragments arranged by size:
1842 1455 627 615 385 383 325 217
77
BanII G_rGCy'C
Cuts at: 1391 2090 2205 3245 1391
Size: 699 115 1040 4072
Fragments arranged by size:
4072 1040 699 115
BbeI G_GCGC'C
Cuts at: 2406 2406
Size: 5926
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2852 2886 4117 4151 1903
Size: 34 915 34 1231 34 3678
Fragments arranged by size:
3678 1231 915 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2711 2949 3023
Size: 107 122 71 631 605 1039 238 74
Cuts at: 3023 4537 136
Size: 1514 1525
Fragments arranged by size:
1525 1514 1039 631 605 238 122 107
74 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 3134 3343 3134
Size: 209 5717
Bme1580I G_kGCm'C
Cuts at: 1790 3195 3987 5143 1790
Size: 1405 792 1156 2573
Fragments arranged by size:
2573 1405 1156 792
BmgBI CAC'GTC
Cuts at: 1318 2752 3190 1318
Size: 1434 438 4054
Fragments arranged by size:
4054 1434 438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3657 4615 442
Size: 416 53 83 399 24 2240 958 1753
Fragments arranged by size:
2240 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2591 2906 4586 1239
Size: 1010 342 315 1680 2579
Fragments arranged by size:
2579 1680 1010 342 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2272 2651 4051 4916 5214 2272
Size: 379 1400 865 298 2984
Fragments arranged by size:
2984 1400 865 379 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4589 882
Size: 617 3090 2219
Fragments arranged by size:
3090 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2108 2403 3733 4115
Size: 53 83 186 936 575 295 1330 382
Cuts at: 4115 4750 275
Size: 635 1451
Fragments arranged by size:
1451 1330 936 635 575 382 295 186
83 53
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5701
Fragments arranged by size:
5701 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2455 3037 3224 1186
Size: 1027 242 582 187 3888
Fragments arranged by size:
3888 1027 582 242 187
BseYI C'CCAG_C
Cuts at: 2029 2068 2848 3013 5129 5625 2029
Size: 39 780 165 2116 496 2330
Fragments arranged by size:
2330 2116 780 496 165 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2774 2858 1163
Size: 573 582 456 84 4231
Fragments arranged by size:
4231 582 573 456 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2023 2061 2209 2362 3376 4137
Size: 275 186 22 38 148 153 1014 761
Cuts at: 4137 5165 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 1014 761 275 186 153 148
38 22
BsiHKAI G_wGCw'C
Cuts at: 2205 3140 3987 4072 5143 2205
Size: 935 847 85 1071 2988
Fragments arranged by size:
2988 1071 935 847 85
BsmI GAATG_Cn'
Cuts at: 2868 3120 3437 3530 2868
Size: 252 317 93 5264
Fragments arranged by size:
5264 317 252 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2205 3140 3195 3245 3987 4072
Size: 399 300 115 935 55 50 742 85
Cuts at: 4072 5143 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 935 742 399 300 115 85
55 50
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3281 844
Size: 2437 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2725 2734 3813 1394
Size: 429 902 9 1079 3507
Fragments arranged by size:
3507 1079 902 429 9
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2274 2583 2788 2820 2978 4570
Size: 110 250 214 309 205 32 158 1592
Cuts at: 4570 4757 1700
Size: 187 2869
Fragments arranged by size:
2869 1592 309 250 214 205 187 158
110 32
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2296 794
Size: 1502 4424
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2141 2370 3256 3623 4008 4025 5477 5563
Size: 169 229 886 367 385 17 1452 86
Cuts at: 5563 5575 5921 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 886 385 367 346 229 169
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2076 2301 2076
Size: 225 5701
BtgI C'CryG_G
Cuts at: 513 1065 2004 2310 2626 2781 513
Size: 552 939 306 316 155 3658
Fragments arranged by size:
3658 939 552 316 306 155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2907 487
Size: 683 56 1681 3506
Fragments arranged by size:
3506 1681 683 56
BtsI GCAGTG_nn'
Cuts at: 2762 3442 4236 4256 2762
Size: 680 794 20 4432
Fragments arranged by size:
4432 794 680 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DraIII CAC_nnn'GTG
Cuts at: 2296 2296
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2192 2206
Size: 54 1425 50 275 18 228 134 14
Cuts at: 2206 2359 2386 2410 2778 2896 3373 4262 5891
Size: 153 27 24 368 118 477 889 1629
Cuts at: 5891 8
Size: 43
Fragments arranged by size:
1629 1425 889 477 368 275 228 153
134 118 54 50 43 27 24 18
14
EagI C'GGCC_G
Cuts at: 1537 1812 2058 2206 2359 3373 1537
Size: 275 246 148 153 1014 4090
Fragments arranged by size:
4090 1014 275 246 153 148
EarI CTCTTCn'nnn_
Cuts at: 1353 2648 2805 3856 1353
Size: 1295 157 1051 3423
Fragments arranged by size:
3423 1295 1051 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2465 2602 2930 4513 4887 5033
Size: 1064 138 876 137 328 1583 374 146
Cuts at: 5033 387
Size: 1280
Fragments arranged by size:
1583 1280 1064 876 374 328 146 138
137
EcoICRI GAG'CTC
Cuts at: 2203 2203
Size: 5926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2591 2906 3989 4586 5373 5641
Size: 393 617 342 315 1083 597 787 268
Cuts at: 5641 1239
Size: 1524
Fragments arranged by size:
1524 1083 787 617 597 393 342 315
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2573
Size: 5926
EcoO109I rG'GnC_Cy
Cuts at: 2072 3031 2072
Size: 959 4967
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FseI GG_CCGG'CC
Cuts at: 2278 2278
Size: 5926
FspI TGC'GCA
Cuts at: 2327 4432 2327
Size: 2105 3821
HaeII r_GCGC'y
Cuts at: 1678 2030 2406 5073 1678
Size: 352 376 2667 2531
Fragments arranged by size:
2667 2531 376 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4567 4599 4641 4673 1373
Size: 32 135 32 2995 32 42 32 2626
Fragments arranged by size:
2995 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2402 2402
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 5040 5224 1305
Size: 1215 2520 184 2007
Fragments arranged by size:
2520 2007 1215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4104
Fragments arranged by size:
4104 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3062 3146 3203 3884 4243 4402 518
Size: 648 1896 84 57 681 359 159 2042
Fragments arranged by size:
2042 1896 681 648 359 159 84 57
MspA1I CmG'CkG
Cuts at: 1606 2676 2766 3009 3342 4019 5167 5412 1606
Size: 1070 90 243 333 677 1148 245 2120
Fragments arranged by size:
2120 1148 1070 677 333 245 243 90
NaeI GCC'GGC
Cuts at: 2062 2276 2585 2062
Size: 214 309 5403
Fragments arranged by size:
5403 309 214
NarI GG'CG_CC
Cuts at: 2403 2403
Size: 5926
NcoI C'CATG_G
Cuts at: 513 1065 2004 2310 513
Size: 552 939 306 4129
Fragments arranged by size:
4129 939 552 306
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 2060 2274 2583 2060
Size: 214 309 5403
Fragments arranged by size:
5403 309 214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 3085 3284 4829 1132
Size: 97 1856 199 1545 2229
Fragments arranged by size:
2229 1856 1545 199 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2538 2776 1108
Size: 1430 238 4258
Fragments arranged by size:
4258 1430 238
PfoI T'CCnGG_A
Cuts at: 2226 2568 2613 2226
Size: 342 45 5539
Fragments arranged by size:
5539 342 45
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3031 3031
Size: 5926
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PstI C_TGCA'G
Cuts at: 830 2265 3278 830
Size: 1435 1013 3478
Fragments arranged by size:
3478 1435 1013
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2766 3009 2766
Size: 243 5683
SacI G_AGCT'C
Cuts at: 2205 2205
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SanDI GG'GwC_CC
Cuts at: 3031 3031
Size: 5926
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2261 3274 4409 5090 5281 826
Size: 219 1216 1013 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 1013 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2711 2711
Size: 5926
SfoI GGC'GCC
Cuts at: 2404 2404
Size: 5926
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 2148 2400 2471 3253 1872
Size: 276 252 71 782 4545
Fragments arranged by size:
4545 782 276 252 71
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2251 2666 4030 4931 5193 820
Size: 197 926 308 415 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 901 415 308 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3085 3284 1132
Size: 1953 199 3774
Fragments arranged by size:
3774 1953 199
SrfI GCCC'GGGC
Cuts at: 2400 2400
Size: 5926
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 2081 2714 2883 1517
Size: 564 633 169 4560
Fragments arranged by size:
4560 633 564 169
StyI C'CwwG_G
Cuts at: 513 1065 2004 2310 3365 513
Size: 552 939 306 1055 3074
Fragments arranged by size:
3074 1055 939 552 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2107 2989 3953 3970 4123 4308 4753
Size: 1255 527 882 964 17 153 185 445
Cuts at: 4753 325
Size: 1498
Fragments arranged by size:
1498 1255 964 882 527 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 3153 3168
Size: 274 80 33 51 1443 630 546 15
Cuts at: 3168 4172 5884 96
Size: 1004 1712 138
Fragments arranged by size:
1712 1443 1004 630 546 274 138 80
51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2983 3015 5886 5918 2983
Size: 32 2871 32 2991
Fragments arranged by size:
2991 2871 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3202 3202
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 2146 2398 2469 3251 1870
Size: 276 252 71 782 4545
Fragments arranged by size:
4545 782 276 252 71
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV
FalI FspAI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SwaI