Restriction map for
pFN21AB8995
(Circular) (Six-base) MAPSORT of: pFN21AB8995.seq Check: 50 from: 1 to: 5941
pFN21AB8995 5941 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2243 3284 1937
Size: 306 1041 4594
Fragments arranged by size:
4594 1041 306
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4004 5388 5656 1632
Size: 2372 1384 268 1917
Fragments arranged by size:
2372 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 3054 4840 5817 1203
Size: 1851 1786 977 1327
Fragments arranged by size:
1851 1786 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 3020 1166
Size: 1854 4087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2072 3038 1184
Size: 888 966 4087
Fragments arranged by size:
4087 966 888
AlwNI CAG_nnn'CTG
Cuts at: 2965 3684 5256 2965
Size: 719 1572 3650
Fragments arranged by size:
3650 1572 719
ApaI G_GGCC'C
Cuts at: 2045 2938 3132 2045
Size: 893 194 4854
Fragments arranged by size:
4854 893 194
ApaLI G'TGCA_C
Cuts at: 3013 3998 5154 3013
Size: 985 1156 3800
Fragments arranged by size:
3800 1156 985
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 3172 3266 1870
Size: 73 172 1057 94 4545
Fragments arranged by size:
4545 1057 172 94 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3262 4717 618
Size: 325 217 627 1475 1455 1842
Fragments arranged by size:
1842 1475 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2938 3132 3173 3192 3260 1391
Size: 654 893 194 41 19 68 4072
Fragments arranged by size:
4072 893 654 194 68 41 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BbvCI CC'TCA_GC
Cuts at: 2192 2192
Size: 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4132 4166 1903
Size: 34 50 34 2111 34 3678
Fragments arranged by size:
3678 2111 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2393 2453 2483
Size: 107 122 71 631 605 721 60 30
Cuts at: 2483 2513 2603 2663 2693 2723 2753 2783 2813
Size: 30 90 60 30 30 30 30 30
Cuts at: 2813 4552 136
Size: 1739 1525
Fragments arranged by size:
1739 1525 721 631 605 122 107 90
71 60 60 30 30 30 30 30
30 30
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2418 2938 3017 3132 4002 5158 1790
Size: 255 373 520 79 115 870 1156 2573
Fragments arranged by size:
2573 1156 870 520 373 255 115 79
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2048 2232 2796
Size: 416 53 83 399 24 631 184 564
Cuts at: 2796 2921 3672 4630 442
Size: 125 751 958 1753
Fragments arranged by size:
1753 958 751 631 564 416 399 184
125 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 2591 4601 1239
Size: 927 425 2010 2579
Fragments arranged by size:
2579 2010 927 425
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 5941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3182 3748 4130 4765
Size: 53 83 186 936 1649 566 382 635
Cuts at: 4765 275
Size: 1451
Fragments arranged by size:
1649 1451 936 635 566 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2121 4374 4772 5046 5193 5604 2121
Size: 2253 398 274 147 411 2458
Fragments arranged by size:
2458 2253 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2164 2194 2283 2313 2774
Size: 30 165 30 761 30 89 30 461
Cuts at: 2774 2804 1178
Size: 30 4315
Fragments arranged by size:
4315 761 461 165 89 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2009 2205 1186
Size: 823 196 4922
Fragments arranged by size:
4922 823 196
BseYI C'CCAG_C
Cuts at: 5144 5640 5144
Size: 496 5445
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2571 2601 2691 3209 1163
Size: 573 835 30 90 518 3895
Fragments arranged by size:
3895 835 573 518 90 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3017 4002 4087 5158 3017
Size: 985 85 1071 3800
Fragments arranged by size:
3800 1071 985 85
BsmI GAATG_Cn'
Cuts at: 2889 3452 3545 2889
Size: 563 93 5285
Fragments arranged by size:
5285 563 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2418 2938 3017 3132 3173 3192
Size: 399 255 373 520 79 115 41 19
Cuts at: 3192 3260 4002 4087 5158 1391
Size: 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 373 255 115
85 79 68 41 19
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2965 794
Size: 2171 3770
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstXI CCAn_nnnn'nTGG
Cuts at: 2320 2440 2500 2569 2689 2770 3088 2320
Size: 120 60 69 120 81 318 5173
Fragments arranged by size:
5173 318 120 120 81 69 60
BstYI r'GATC_y
Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936
Size: 1299 367 385 17 1452 86 12 346
Cuts at: 5936 1972
Size: 1977
Fragments arranged by size:
1977 1452 1299 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2313 2343 2388 2418 2478 2538 2598
Size: 552 1248 30 45 30 60 60 60
Cuts at: 2598 2628 2658 2688 2718 2748 2778 2823 2895
Size: 30 30 30 30 30 30 45 72
Cuts at: 2895 513
Size: 3559
Fragments arranged by size:
3559 1248 552 72 60 60 60 45
45 30 30 30 30 30 30 30
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2583 2613 2703 2966 3457 4251 4271 2583
Size: 30 90 263 491 794 20 4253
Fragments arranged by size:
4253 794 491 263 90 30 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2475 2970
Size: 54 1425 50 275 18 492 153 495
Cuts at: 2970 2994 3388 4277 5906 8
Size: 24 394 889 1629 43
Fragments arranged by size:
1629 1425 889 495 492 394 275 153
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3871 1353
Size: 2518 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3023 4528 4902 5048 387
Size: 1064 138 1434 1505 374 146 1280
Fragments arranged by size:
1505 1434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2591 4004 4601 5388 5656 1239
Size: 393 534 425 1413 597 787 268 1524
Fragments arranged by size:
1524 1413 787 597 534 425 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3158 3158
Size: 5941
EcoO109I rG'GnC_Cy
Cuts at: 2042 2102 2785 2815 2934 2935 3128 3129 3151
Size: 60 683 30 119 1 193 1 22
Cuts at: 3151 2042
Size: 4832
Fragments arranged by size:
4832 683 193 119 60 30 22 1
1
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2990 5088 1678
Size: 1312 2098 2531
Fragments arranged by size:
2531 2098 1312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2315 2774 2804 2806
Size: 32 135 32 711 32 459 30 2
Cuts at: 2806 2836 2933 2965 4582 4614 4656 4688 1373
Size: 30 97 32 1617 32 42 32 2626
Fragments arranged by size:
2626 1617 711 459 135 97 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2920 3285 3531 669
Size: 988 281 982 365 246 3079
Fragments arranged by size:
3079 988 982 365 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2324 2972 2996 10
Size: 54 1425 343 492 648 24 2955
Fragments arranged by size:
2955 1425 648 492 343 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 2894 2951 3020 3086 3899 4258
Size: 648 1521 207 57 69 66 813 359
Cuts at: 4258 4417 518
Size: 159 2042
Fragments arranged by size:
2042 1521 813 648 359 207 159 69
66 57
MspA1I CmG'CkG
Cuts at: 1606 2062 3357 4034 5182 5427 1606
Size: 456 1295 677 1148 245 2120
Fragments arranged by size:
2120 1295 1148 677 456 245
NcoI C'CATG_G
Cuts at: 513 1065 2598 2628 2658 2688 2778 2823 2895
Size: 552 1533 30 30 30 90 45 72
Cuts at: 2895 513
Size: 3559
Fragments arranged by size:
3559 1533 552 90 72 45 30 30
30
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3058 3299 4844 1132
Size: 97 1829 241 1545 2229
Fragments arranged by size:
2229 1829 1545 241 97
PasI CC'CwG_GG
Cuts at: 1348 3192 1348
Size: 1844 4097
PciI A'CATG_T
Cuts at: 3054 4840 3054
Size: 1786 4155
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2102 2102
Size: 5941
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2041 2934 3128 2041
Size: 893 194 4854
Fragments arranged by size:
4854 893 194
PstI C_TGCA'G
Cuts at: 830 3293 830
Size: 2463 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2062 2062
Size: 5941
RsrII CG'GwC_CG
Cuts at: 3031 3031
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2102 2102
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 3289 4424 5105 5296 826
Size: 219 2244 1135 681 191 1471
Fragments arranged by size:
2244 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2117 3174 3268 1872
Size: 245 1057 94 4545
Fragments arranged by size:
4545 1057 245 94
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4045 4946 5208 820
Size: 197 926 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SrfI GCCC'GGGC
Cuts at: 3174 3174
Size: 5941
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 2568 1517
Size: 1051 4890
StyI C'CwwG_G
Cuts at: 513 1065 2097 2598 2628 2658 2688 2778 2823
Size: 552 1032 501 30 30 30 90 45
Cuts at: 2823 2895 3159 3380 513
Size: 72 264 221 3074
Fragments arranged by size:
3074 1032 552 501 264 221 90 72
45 30 30 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3968 3985 4138 4323 4768 325
Size: 1255 2388 17 153 185 445 1498
Fragments arranged by size:
2388 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4187 5899 96
Size: 274 80 33 51 1443 2210 1712 138
Fragments arranged by size:
2210 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2980 2980
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2115 3172 3266 1870
Size: 245 1057 94 4545
Fragments arranged by size:
4545 1057 245 94
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI SacI SacII SapI
SexAI SfiI SfoI SwaI