Restriction map for
pFN21AB8993
(Circular) (Six-base) MAPSORT of: pFN21AB8993.seq Check: 1312 from: 1 to: 5908
pFN21AB8993 5908 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3251 1937
Size: 1314 4594
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 3207 4035 4408 1563
Size: 1644 828 373 3063
Fragments arranged by size:
3063 1644 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2004 2046 2718 3971 5355 5623 1632
Size: 372 42 672 1253 1384 268 1917
Fragments arranged by size:
1917 1384 1253 672 372 268 42
AflII C'TTAA_G
Cuts at: 820 1017 2509 820
Size: 197 1492 4219
Fragments arranged by size:
4219 1492 197
AflIII A'CryG_T
Cuts at: 1203 2011 4807 5784 1203
Size: 808 2796 977 1327
Fragments arranged by size:
2796 1327 977 808
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 2122 4637 2122
Size: 2515 3393
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2085
Size: 32 5876
AlwNI CAG_nnn'CTG
Cuts at: 3651 5223 3651
Size: 1572 4336
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 2349 2439 2449 2539 2734 2785 2950 3217
Size: 856 90 10 90 195 51 165 267
Cuts at: 3217 3443 3595 5704 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 856 267 226 195 165 152
90 90 51 10
AseI AT'TA_AT
Cuts at: 160 1032 2325 4462 160
Size: 872 1293 2137 1606
Fragments arranged by size:
2137 1606 1293 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 3233 1870
Size: 73 1290 4545
Fragments arranged by size:
4545 1290 73
BamHI G'GATC_C
Cuts at: 2165 3238 3605 2165
Size: 1073 367 4468
Fragments arranged by size:
4468 1073 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3229 4684 618
Size: 325 217 627 1442 1455 1842
Fragments arranged by size:
1842 1455 1442 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3227 1391
Size: 1836 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4099 4133 1903
Size: 34 2162 34 3678
Fragments arranged by size:
3678 2162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4519 136
Size: 107 122 71 631 605 2847 1525
Fragments arranged by size:
2847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2459 5903 2459
Size: 3444 2464
BlpI GC'TnA_GC
Cuts at: 3325 3325
Size: 5908
Bme1580I G_kGCm'C
Cuts at: 1790 3969 5125 1790
Size: 2179 1156 2573
Fragments arranged by size:
2573 2179 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3639 4597 442
Size: 416 53 83 399 24 2222 958 1753
Fragments arranged by size:
2222 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3164 1056
Size: 2108 3800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4568 1239
Size: 3329 2579
Bpu10I CC'TnA_GC
Cuts at: 2689 2689
Size: 5908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2013 4033 4898 5196 2013
Size: 2020 865 298 2725
Fragments arranged by size:
2725 2020 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2085 4571 882
Size: 617 586 2486 2219
Fragments arranged by size:
2486 2219 617 586
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 5902 5902
Size: 5908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3715 4097 4732 275
Size: 53 83 186 936 2182 382 635 1451
Fragments arranged by size:
2182 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4341 4739 5013 5160 5571 4341
Size: 398 274 147 411 4678
Fragments arranged by size:
4678 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 2350 2380 1178
Size: 30 165 30 662 30 255 30 4706
Fragments arranged by size:
4706 662 255 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2808 1186
Size: 1622 4286
BseYI C'CCAG_C
Cuts at: 5111 5607 5111
Size: 496 5412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3129 3358 4119 5147 1540
Size: 275 186 1128 229 761 1028 2301
Fragments arranged by size:
2301 1128 1028 761 275 229 186
BsiHKAI G_wGCw'C
Cuts at: 3969 4054 5125 3969
Size: 85 1071 4752
Fragments arranged by size:
4752 1071 85
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3227 3969 4054 5125 1391
Size: 399 1437 742 85 1071 2174
Fragments arranged by size:
2174 1437 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5571 5571
Size: 5908
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3263 844
Size: 2419 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3795 1394
Size: 429 1972 3507
Fragments arranged by size:
3507 1972 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4403 4577 65
Size: 1183 3155 174 1396
Fragments arranged by size:
3155 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4552 4739 1700
Size: 110 2742 187 2869
Fragments arranged by size:
2869 2742 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5908
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5908
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 2165 2459 3238 3605 3990 4007 5459 5545
Size: 193 294 779 367 385 17 1452 86
Cuts at: 5545 5557 5903 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 779 385 367 346 294 193
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3107 513
Size: 552 939 1103 3314
Fragments arranged by size:
3314 1103 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5169
Fragments arranged by size:
5169 683 56
BtsI GCAGTG_nn'
Cuts at: 3424 4218 4238 3424
Size: 794 20 5094
Fragments arranged by size:
5094 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 2739 2883 3212 2739
Size: 144 329 5435
Fragments arranged by size:
5435 329 144
DraIII CAC_nnn'GTG
Cuts at: 2947 2947
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4915 809
Size: 507 3599 1802
Fragments arranged by size:
3599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873
Size: 54 1425 50 275 18 1525 889 1629
Cuts at: 5873 8
Size: 43
Fragments arranged by size:
1629 1525 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3355 1537
Size: 275 1543 4090
Fragments arranged by size:
4090 1543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 2484 2573 3171 3838 1353
Size: 989 142 89 598 667 3423
Fragments arranged by size:
3423 989 667 598 142 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4495 4869 5015 387
Size: 1064 138 2906 374 146 1280
Fragments arranged by size:
2906 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2046 2718 3971 4568 5355 5623
Size: 393 372 42 672 1253 597 787 268
Cuts at: 5623 1239
Size: 1524
Fragments arranged by size:
1524 1253 787 672 597 393 372 268
42
EcoNI CCTnn'n_nnAGG
Cuts at: 2074 2074
Size: 5908
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
EcoRV GAT'ATC
Cuts at: 3050 3050
Size: 5908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2778 2778
Size: 5908
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 5055 1678
Size: 3377 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2483 2515 4549
Size: 32 135 32 536 32 343 32 2034
Cuts at: 4549 4581 4623 4655 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2034 536 343 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3252 3498 669
Size: 988 281 954 360 246 3079
Fragments arranged by size:
3079 988 954 360 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2821 5022 5206 1305
Size: 1516 2201 184 2007
Fragments arranged by size:
2201 2007 1516 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3866 4225 4384 518
Size: 648 2700 359 159 2042
Fragments arranged by size:
2700 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3143 3324 4001 5149 5394 1606
Size: 1537 181 677 1148 245 2120
Fragments arranged by size:
2120 1537 1148 677 245 181
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4417
Fragments arranged by size:
4417 939 552
NdeI CA'TA_TG
Cuts at: 387 2904 387
Size: 2517 3391
NheI G'CTAG_C
Cuts at: 1052 3160 1052
Size: 2108 3800
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 2015 2144 2876 2929 3091 3266 4811
Size: 97 786 129 732 53 162 175 1545
Cuts at: 4811 1132
Size: 2229
Fragments arranged by size:
2229 1545 786 732 175 162 129 97
53
PasI CC'CwG_GG
Cuts at: 1348 2105 1348
Size: 757 5151
PciI A'CATG_T
Cuts at: 2011 4807 2011
Size: 2796 3112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 2532 3478 2532
Size: 946 4962
PstI C_TGCA'G
Cuts at: 830 3260 830
Size: 2430 3478
PvuI CG_AT'CG
Cuts at: 2001 3129 2001
Size: 1128 4780
PvuII CAG'CTG
Cuts at: 3143 3143
Size: 5908
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SapI GCTCTTCn'nnn_
Cuts at: 3171 3171
Size: 5908
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SfcI C'TryA_G
Cuts at: 826 1045 3256 4391 5072 5263 826
Size: 219 2211 1135 681 191 1471
Fragments arranged by size:
2211 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 3235 1872
Size: 1363 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2028 2509 4012 4913 5175 820
Size: 197 926 85 481 1503 901 262 1553
Fragments arranged by size:
1553 1503 926 901 481 262 197 85
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SspI AAT'ATT
Cuts at: 5 52 2379 3832 5
Size: 47 2327 1453 2081
Fragments arranged by size:
2327 2081 1453 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5908
StyI C'CwwG_G
Cuts at: 513 1065 2004 3347 513
Size: 552 939 1343 3074
Fragments arranged by size:
3074 1343 939 552
SwaI ATTT'AAAT
Cuts at: 2739 2739
Size: 5908
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3935 3952 4105 4290 4735 325
Size: 1255 2355 17 153 185 445 1498
Fragments arranged by size:
2355 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4154 5866 96
Size: 274 80 33 51 1443 2177 1712 138
Fragments arranged by size:
2177 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2065 2097 3034 3066 5868 5900 2065
Size: 32 937 32 2802 32 2073
Fragments arranged by size:
2802 2073 937 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5908
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5908
XmaI C'CCGG_G
Cuts at: 1870 3233 1870
Size: 1363 4545
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
XcmI