Restriction map for
pFN21AB8990
(Circular) (Six-base) MAPSORT of: pFN21AB8990.seq Check: 8214 from: 1 to: 5878
pFN21AB8990 5878 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2740 2740
Size: 5878
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3941 5325 5593 1632
Size: 2309 1384 268 1917
Fragments arranged by size:
2309 1917 1384 268
AfeI AGC'GCT
Cuts at: 3017 3017
Size: 5878
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 4777 5754 1203
Size: 3574 977 1327
Fragments arranged by size:
3574 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AlwNI CAG_nnn'CTG
Cuts at: 2439 2755 3621 5193 2439
Size: 316 866 1572 3124
Fragments arranged by size:
3124 1572 866 316
ApaI G_GGCC'C
Cuts at: 2112 2112
Size: 5878
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 3187 3413 3565 5674 1493
Size: 1694 226 152 2109 1697
Fragments arranged by size:
2109 1697 1694 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 3203 1870
Size: 73 1260 4545
Fragments arranged by size:
4545 1260 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3199 4654 618
Size: 325 217 627 1412 1455 1842
Fragments arranged by size:
1842 1455 1412 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2112 2573 3197 1391
Size: 721 461 624 4072
Fragments arranged by size:
4072 721 624 461
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2239 928
Size: 971 340 4567
Fragments arranged by size:
4567 971 340
BbvCI CC'TCA_GC
Cuts at: 2240 2240
Size: 5878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2875 3770 4980 2875
Size: 895 1210 3773
Fragments arranged by size:
3773 1210 895
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2283 5873 2283
Size: 3590 2288
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2112 3110 3939 5095 1790
Size: 271 51 998 829 1156 2573
Fragments arranged by size:
2573 1156 998 829 271 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3169 3609 4567
Size: 416 53 83 399 24 1752 440 958
Cuts at: 4567 442
Size: 1753
Fragments arranged by size:
1753 1752 958 440 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2893 4538 1239
Size: 1654 1645 2579
Fragments arranged by size:
2579 1654 1645
Bpu10I CC'TnA_GC
Cuts at: 2240 2567 2240
Size: 327 5551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4003 4868 5166 4003
Size: 865 298 4715
Fragments arranged by size:
4715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4541 882
Size: 617 3042 2219
Fragments arranged by size:
3042 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3001 3669 493
Size: 711 1797 668 2702
Fragments arranged by size:
2702 1797 711 668
BsaBI GATnn'nnATC
Cuts at: 2741 5872 2741
Size: 3131 2747
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2589 4311 4709 4983 5130 5541 2589
Size: 1722 398 274 147 411 2926
Fragments arranged by size:
2926 1722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5653
Fragments arranged by size:
5653 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2815 1186
Size: 1629 4249
BseYI C'CCAG_C
Cuts at: 2161 2520 5081 5577 2161
Size: 359 2561 496 2462
Fragments arranged by size:
2561 2462 496 359
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2240 2254 2716 1163
Size: 573 504 14 462 4325
Fragments arranged by size:
4325 573 504 462 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2467 3328 4089 5117 1540
Size: 275 186 466 861 761 1028 2301
Fragments arranged by size:
2301 1028 861 761 466 275 186
BsiHKAI G_wGCw'C
Cuts at: 2573 3939 4024 5095 2573
Size: 1366 85 1071 3356
Fragments arranged by size:
3356 1366 1071 85
BsmI GAATG_Cn'
Cuts at: 2410 3389 3482 2410
Size: 979 93 4806
Fragments arranged by size:
4806 979 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2378 2378
Size: 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2112 2573 3110 3197 3939 4024
Size: 399 271 51 461 537 87 742 85
Cuts at: 4024 5095 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 537 461 399 271 87
85 51
BspEI T'CCGG_A
Cuts at: 2589 5541 2589
Size: 2952 2926
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2740 3063 3233 844
Size: 1896 323 170 3489
Fragments arranged by size:
3489 1896 323 170
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 4547 65
Size: 1183 3125 174 1396
Fragments arranged by size:
3125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4522 4709 1700
Size: 110 2712 187 2869
Fragments arranged by size:
2869 2712 187 110
BsrGI T'GTAC_A
Cuts at: 96 2427 96
Size: 2331 3547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 3938 4950 3938
Size: 1012 4866
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2746 2755 2979 794
Size: 1952 9 224 3693
Fragments arranged by size:
3693 1952 224 9
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 3091 3130 2497
Size: 594 39 5245
Fragments arranged by size:
5245 594 39
BstYI r'GATC_y
Cuts at: 1972 2283 2787 3208 3575 3960 3977 5429 5515
Size: 311 504 421 367 385 17 1452 86
Cuts at: 5515 5527 5873 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 504 421 385 367 346 311
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2064 2496 2808 3102 3129 513
Size: 552 999 432 312 294 27 3262
Fragments arranged by size:
3262 999 552 432 312 294 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2625 2876 487
Size: 683 56 1399 251 3489
Fragments arranged by size:
3489 1399 683 251 56
BtsI GCAGTG_nn'
Cuts at: 3110 3394 4188 4208 3110
Size: 284 794 20 4780
Fragments arranged by size:
4780 794 284 20
ClaI AT'CG_AT
Cuts at: 1554 2934 1554
Size: 1380 4498
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2138 2173 529
Size: 35 1574 35 4234
Fragments arranged by size:
4234 1574 35 35
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2301 3325
Size: 54 1425 50 275 18 384 87 1024
Cuts at: 3325 4214 5843 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1024 889 384 275 87 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3808 1353
Size: 2455 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 4839 4985 387
Size: 1064 138 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2571 2571
Size: 5878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2893 3941 4538 5325 5593 1239
Size: 393 1261 1048 597 787 268 1524
Fragments arranged by size:
1524 1261 1048 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2108 2124 2108
Size: 16 5862
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
EcoRV GAT'ATC
Cuts at: 2025 2025
Size: 5878
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 2082 3019 5025 1678
Size: 404 937 2006 2531
Fragments arranged by size:
2531 2006 937 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373
Size: 32 135 32 2947 32 42 32 2626
Fragments arranged by size:
2947 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 4992 5176 1305
Size: 1077 2610 184 2007
Fragments arranged by size:
2610 2007 1077 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 10
Size: 54 1425 343 384 3672
Fragments arranged by size:
3672 1425 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2582 2678 2750 3089 3128 3836
Size: 648 1329 87 96 72 339 39 708
Cuts at: 3836 4195 4354 518
Size: 359 159 2042
Fragments arranged by size:
2042 1329 708 648 359 339 159 96
87 72 39
MspA1I CmG'CkG
Cuts at: 1606 2268 2913 2979 3294 3971 5119 5364 1606
Size: 662 645 66 315 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 662 645 315 245 66
NcoI C'CATG_G
Cuts at: 513 1065 2496 3102 513
Size: 552 1431 606 3289
Fragments arranged by size:
3289 1431 606 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 2089 2683 3236 4781 1132
Size: 97 860 594 553 1545 2229
Fragments arranged by size:
2229 1545 860 594 553 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 2877 2877
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2124 2124
Size: 5878
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PspOMI G'GGCC_C
Cuts at: 2108 2108
Size: 5878
PstI C_TGCA'G
Cuts at: 830 3074 3156 3230 830
Size: 2244 82 74 3478
Fragments arranged by size:
3478 2244 82 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 2268 2913 2979 2268
Size: 645 66 5167
Fragments arranged by size:
5167 645 66
SacI G_AGCT'C
Cuts at: 2573 2573
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 2533 2968 3070 3152 3226 4361 5042
Size: 219 1488 435 102 82 74 1135 681
Cuts at: 5042 5233 826
Size: 191 1471
Fragments arranged by size:
1488 1471 1135 681 435 219 191 102
82 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3982 4883 5145 820
Size: 197 926 2039 901 262 1553
Fragments arranged by size:
2039 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2496 3102 3317 513
Size: 552 1431 606 215 3074
Fragments arranged by size:
3074 1431 606 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 2590 3905 3922 4075 4260 4705
Size: 1255 608 402 1315 17 153 185 445
Cuts at: 4705 325
Size: 1498
Fragments arranged by size:
1498 1315 1255 608 445 402 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 2604 4124
Size: 274 80 33 51 1443 450 177 1520
Cuts at: 4124 5836 96
Size: 1712 138
Fragments arranged by size:
1712 1520 1443 450 274 177 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 5846
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2658 2658
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 3084 4007 3084
Size: 923 4955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI