Restriction map for
pFN21AB8989
(Circular) (Six-base) MAPSORT of: pFN21AB8989.seq Check: 6181 from: 1 to: 5875
pFN21AB8989 5875 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2882 3685 278
Size: 53 83 186 936 1346 803 2468
Fragments arranged by size:
2468 1346 936 803 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 2949 3218 1937
Size: 262 750 269 4594
Fragments arranged by size:
4594 750 269 262
Acc65I G'GTAC_C
Cuts at: 2049 3196 2049
Size: 1147 4728
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2894 3938 5322 5590 1632
Size: 1262 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1262 1044 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2696 1184
Size: 1512 4363
AlwNI CAG_nnn'CTG
Cuts at: 3618 5190 3618
Size: 1572 4303
ApaLI G'TGCA_C
Cuts at: 3110 3932 5088 3110
Size: 822 1156 3897
Fragments arranged by size:
3897 1156 822
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2579 3200 1870
Size: 73 102 534 621 4545
Fragments arranged by size:
4545 621 534 102 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 2445 2848 2956 3038
Size: 325 217 627 262 396 403 108 82
Cuts at: 3038 3196 4651 618
Size: 158 1455 1842
Fragments arranged by size:
1842 1455 627 403 396 325 262 217
158 108 82
BanII G_rGCy'C
Cuts at: 1391 2092 3194 1391
Size: 701 1102 4072
Fragments arranged by size:
4072 1102 701
BbeI G_GCGC'C
Cuts at: 2449 3042 2449
Size: 593 5282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2375 2738 2771 2804 2882 928
Size: 971 476 363 33 33 78 3921
Fragments arranged by size:
3921 971 476 363 78 33 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 2133 1548
Size: 585 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 2168 2282 3292 2168
Size: 114 1010 4751
Fragments arranged by size:
4751 1010 114
Bme1580I G_kGCm'C
Cuts at: 1790 2840 2853 3114 3936 5092 1790
Size: 1050 13 261 822 1156 2573
Fragments arranged by size:
2573 1156 1050 822 261 13
BmgBI CAC'GTC
Cuts at: 1318 2977 3109 1318
Size: 1659 132 4084
Fragments arranged by size:
4084 1659 132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2357 2408 2438 2621 2780 2813 2834
Size: 1010 108 51 30 183 159 33 21
Cuts at: 2834 3143 4535 1239
Size: 309 1392 2579
Fragments arranged by size:
2579 1392 1010 309 183 159 108 51
33 30 21
Bpu10I CC'TnA_GC
Cuts at: 2091 2091
Size: 5875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2202 2759 2996 4000 4865 5163 2202
Size: 557 237 1004 865 298 2914
Fragments arranged by size:
2914 1004 865 557 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2920 3666 493
Size: 711 1716 746 2702
Fragments arranged by size:
2702 1716 746 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2446 2879 3039
Size: 53 83 186 936 525 388 433 160
Cuts at: 3039 3144 3682 4064 4699 275
Size: 105 538 382 635 1451
Fragments arranged by size:
1451 936 635 538 525 433 388 382
186 160 105 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2093 2123 2594 2624 2918
Size: 30 165 30 690 30 471 30 294
Cuts at: 2918 2948 1178
Size: 30 4105
Fragments arranged by size:
4105 690 471 294 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2246 2570 2597 2648 2660 3149 1186
Size: 931 129 324 27 51 12 489 3912
Fragments arranged by size:
3912 931 489 324 129 51 27 12
BseYI C'CCAG_C
Cuts at: 2084 2290 2560 2833 5078 5574 2084
Size: 206 270 273 2245 496 2385
Fragments arranged by size:
2385 2245 496 273 270 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 1163
Size: 573 546 4756
Fragments arranged by size:
4756 573 546
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2039 3325 4086 5114 1540
Size: 275 186 38 1286 761 1028 2301
Fragments arranged by size:
2301 1286 1028 761 275 186 38
BsiHKAI G_wGCw'C
Cuts at: 3012 3114 3936 4021 5092 3012
Size: 102 822 85 1071 3795
Fragments arranged by size:
3795 1071 822 102 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2840 2853 3012 3114 3194 3936
Size: 399 302 748 13 159 102 80 742
Cuts at: 3936 4021 5092 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 748 742 399 302 159 102
85 80 13
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 2463 3764 5494 2463
Size: 1301 1730 2844
Fragments arranged by size:
2844 1730 1301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3093 3230 844
Size: 2249 137 3489
Fragments arranged by size:
3489 2249 137
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 2236 2575 2860 3762 1394
Size: 429 212 201 339 285 902 3507
Fragments arranged by size:
3507 902 429 339 285 212 201
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2117 2491 3077 4519 4706 1700
Size: 110 307 374 586 1442 187 2869
Fragments arranged by size:
2869 1442 586 374 307 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 2930 1925
Size: 1005 4870
BssSI C'ACGA_G
Cuts at: 2311 3935 4947 2311
Size: 1624 1012 3239
Fragments arranged by size:
3239 1624 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3118 794
Size: 2324 3551
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstXI CCAn_nnnn'nTGG
Cuts at: 3025 3025
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2205 2985 3205 3572 3957 3974 5426 5512
Size: 233 780 220 367 385 17 1452 86
Cuts at: 5512 5524 5870 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 780 385 367 346 233 220
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2943 513
Size: 552 939 939 3445
Fragments arranged by size:
3445 939 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 3391 4185 4205 2265
Size: 1126 794 20 3935
Fragments arranged by size:
3935 1126 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 2458 3179 2458
Size: 721 5154
DraIII CAC_nnn'GTG
Cuts at: 2923 2923
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 2493 3322
Size: 54 1425 50 275 18 206 457 829
Cuts at: 3322 4211 5840 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 829 457 275 206 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2036 3322 1537
Size: 275 224 1286 4090
Fragments arranged by size:
4090 1286 275 224
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 3805 1353
Size: 768 1684 3423
Fragments arranged by size:
3423 1684 768
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2258 4462 4836 4982 387
Size: 1064 138 669 2204 374 146 1280
Fragments arranged by size:
2204 1280 1064 669 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2357 2408 2438 2621 2780 2813
Size: 393 617 108 51 30 183 159 33
Cuts at: 2813 2834 2894 3143 3938 4535 5322 5590 1239
Size: 21 60 249 795 597 787 268 1524
Fragments arranged by size:
1524 795 787 617 597 393 268 249
183 159 108 60 51 33 30 21
EcoO109I rG'GnC_Cy
Cuts at: 2011 2065 2011
Size: 54 5821
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 5875
FspI TGC'GCA
Cuts at: 2348 2897 4381 2348
Size: 549 1484 3842
Fragments arranged by size:
3842 1484 549
HaeII r_GCGC'y
Cuts at: 1678 2395 2449 2537 2600 3042 5022 1678
Size: 717 54 88 63 442 1980 2531
Fragments arranged by size:
2531 1980 717 442 88 63 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2594 2626 4516
Size: 32 135 32 521 32 469 32 1890
Cuts at: 4516 4548 4590 4622 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1890 521 469 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 2425 3219 3465 669
Size: 988 281 424 63 794 246 3079
Fragments arranged by size:
3079 988 794 424 281 246 63
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KasI G'GCGC_C
Cuts at: 2445 3038 2445
Size: 593 5282
KpnI G_GTAC'C
Cuts at: 2053 3200 2053
Size: 1147 4728
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2508 2592 3120 4989 5173 1305
Size: 816 387 84 528 1869 184 2007
Fragments arranged by size:
2007 1869 816 528 387 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4053
Fragments arranged by size:
4053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3023 3833 4192 4351 518
Size: 648 1857 810 359 159 2042
Fragments arranged by size:
2042 1857 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2099 2212 2227 2594 2676 2945 3291
Size: 478 15 113 15 367 82 269 346
Cuts at: 3291 3968 5116 5361 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 478 367 346 269 245
113 82 15 15
NaeI GCC'GGC
Cuts at: 2119 2493 3079 2119
Size: 374 586 4915
Fragments arranged by size:
4915 586 374
NarI GG'CG_CC
Cuts at: 2446 3039 2446
Size: 593 5282
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4384
Fragments arranged by size:
4384 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NgoMIV G'CCGG_C
Cuts at: 2117 2491 3077 2117
Size: 374 586 4915
Fragments arranged by size:
4915 586 374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 2036 3322 2036
Size: 1286 4589
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PfoI T'CCnGG_A
Cuts at: 2175 2175
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PmlI CAC'GTG
Cuts at: 2920 2920
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2011
Size: 5875
PshAI GACnn'nnGTC
Cuts at: 1613 2945 1613
Size: 1332 4543
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PstI C_TGCA'G
Cuts at: 830 3227 830
Size: 2397 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2212 2227 2212
Size: 15 5860
SacII CC_GC'GG
Cuts at: 2946 2946
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SapI GCTCTTCn'nnn_
Cuts at: 2121 2121
Size: 5875
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SexAI A'CCwGG_T
Cuts at: 2145 2145
Size: 5875
SfcI C'TryA_G
Cuts at: 826 1045 3223 4358 5039 5230 826
Size: 219 2178 1135 681 191 1471
Fragments arranged by size:
2178 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2447 3040 2447
Size: 593 5282
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 2047 3202 1872
Size: 175 1155 4545
Fragments arranged by size:
4545 1155 175
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2217 2774 3011 3979 4880 5142
Size: 197 926 274 557 237 968 901 262
Cuts at: 5142 820
Size: 1553
Fragments arranged by size:
1553 968 926 901 557 274 262 237
197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 2708 1517
Size: 1191 4684
StyI C'CwwG_G
Cuts at: 513 1065 2004 2715 2736 2781 3314 513
Size: 552 939 711 21 45 533 3074
Fragments arranged by size:
3074 939 711 552 533 45 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3902 3919 4072 4257 4702 325
Size: 1255 2322 17 153 185 445 1498
Fragments arranged by size:
2322 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4121 5833 96
Size: 274 80 33 51 1443 2144 1712 138
Fragments arranged by size:
2144 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5835 5867 5835
Size: 32 5843
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2788 3025 2722
Size: 66 237 5572
Fragments arranged by size:
5572 237 66
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 2045 3200 1870
Size: 175 1155 4545
Fragments arranged by size:
4545 1155 175
XmnI GAAnn'nnTTC
Cuts at: 2708 4004 2708
Size: 1296 4579
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2880 3683 276
Size: 53 83 186 936 1346 803 2468
Fragments arranged by size:
2468 1346 936 803 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SanDI SfiI SrfI SwaI