Restriction map for
pFN21AB8985
(Circular) (Six-base) MAPSORT of: pFN21AB8985.seq Check: 3916 from: 1 to: 5866
pFN21AB8985 5866 bp
With 182 enzymes: SgfI *
October 20, 2009 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2898 3167 2898
Size: 269 5597
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2378 3676 278
Size: 53 83 186 936 842 1298 2468
Fragments arranged by size:
2468 1298 936 842 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2337 2929 3929 5313 5581 1632
Size: 705 592 1000 1384 268 1917
Fragments arranged by size:
1917 1384 1000 705 592 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 2829 4765 5742 1203
Size: 1626 1936 977 1327
Fragments arranged by size:
1936 1626 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 3609 5181 3609
Size: 1572 4294
ApaLI G'TGCA_C
Cuts at: 3105 3923 5079 3105
Size: 818 1156 3892
Fragments arranged by size:
3892 1156 818
ApoI r'AATT_y
Cuts at: 1493 3175 3401 3553 5662 1493
Size: 1682 226 152 2109 1697
Fragments arranged by size:
2109 1697 1682 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2504 2725 3191 1870
Size: 73 138 423 221 466 4545
Fragments arranged by size:
4545 466 423 221 138 73
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2295 2341 2886 3124 3187
Size: 325 217 627 508 46 545 238 63
Cuts at: 3187 4642 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 545 508 325 238 217
63 46
BanII G_rGCy'C
Cuts at: 1391 2475 2511 2618 3185 1391
Size: 1084 36 107 567 4072
Fragments arranged by size:
4072 1084 567 107 36
BbeI G_GCGC'C
Cuts at: 3128 3128
Size: 5866
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2436 3758 4968 2436
Size: 1322 1210 3334
Fragments arranged by size:
3334 1322 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 2773 4477
Size: 107 122 71 631 605 874 227 1704
Cuts at: 4477 136
Size: 1525
Fragments arranged by size:
1704 1525 874 631 605 227 122 107
71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 2363 3283 2363
Size: 920 4946
Bme1580I G_kGCm'C
Cuts at: 1790 2346 2837 3109 3927 5083 1790
Size: 556 491 272 818 1156 2573
Fragments arranged by size:
2573 1156 818 556 491 272
BmgBI CAC'GTC
Cuts at: 1318 2413 1318
Size: 1095 4771
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2708 4526 1239
Size: 1469 1818 2579
Fragments arranged by size:
2579 1818 1469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 3056 3991 4856 5154 2747
Size: 309 935 865 298 3459
Fragments arranged by size:
3459 935 865 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2684 4529 882
Size: 617 1185 1845 2219
Fragments arranged by size:
2219 1845 1185 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2375 3125 3673 4055
Size: 53 83 186 936 842 750 548 382
Cuts at: 4055 4690 275
Size: 635 1451
Fragments arranged by size:
1451 936 842 750 635 548 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2623 4299 4697 4971 5118 5529 2623
Size: 1676 398 274 147 411 2960
Fragments arranged by size:
2960 1676 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 2204 2234 2408
Size: 30 165 30 693 30 78 30 174
Cuts at: 2408 2438 2600 2630 1178
Size: 30 162 30 4414
Fragments arranged by size:
4414 693 174 165 162 78 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 4953
BseYI C'CCAG_C
Cuts at: 2093 2745 5069 5565 2093
Size: 652 2324 496 2394
Fragments arranged by size:
2394 2324 652 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 1163
Size: 573 678 4615
Fragments arranged by size:
4615 678 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2841 2854 3316 4077 5105 1540
Size: 275 186 840 13 462 761 1028 2301
Fragments arranged by size:
2301 1028 840 761 462 275 186 13
BsiHKAI G_wGCw'C
Cuts at: 2631 3109 3927 4012 5083 2631
Size: 478 818 85 1071 3414
Fragments arranged by size:
3414 1071 818 478 85
BsmI GAATG_Cn'
Cuts at: 2702 3377 3470 2702
Size: 675 93 5098
Fragments arranged by size:
5098 675 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2475 2511 2618 2631 2837 3109
Size: 399 556 129 36 107 13 206 272
Cuts at: 3109 3185 3927 4012 5083 1391
Size: 76 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 399 272 206 129
107 85 76 36 13
BspEI T'CCGG_A
Cuts at: 2623 5529 2623
Size: 2906 2960
BspHI T'CATG_A
Cuts at: 2319 3755 5485 2319
Size: 1436 1730 2700
Fragments arranged by size:
2700 1730 1436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2898 3167 3221 844
Size: 2054 269 54 3489
Fragments arranged by size:
3489 2054 269 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2755 2812 3753 1394
Size: 429 932 57 941 3507
Fragments arranged by size:
3507 941 932 429 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 4361 4535 65
Size: 1183 3113 174 1396
Fragments arranged by size:
3113 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2032 2327 2545 2793 4510 4697
Size: 110 199 23 295 218 248 1717 187
Cuts at: 4697 1700
Size: 2869
Fragments arranged by size:
2869 1717 295 248 218 199 187 110
23
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 2097 3032 1925
Size: 172 935 4759
Fragments arranged by size:
4759 935 172
BssSI C'ACGA_G
Cuts at: 3926 4938 3926
Size: 1012 4854
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3113 794
Size: 2319 3547
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 2035 3151 1184
Size: 851 1116 3899
Fragments arranged by size:
3899 1116 851
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 2611
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 2088 2718 2928 3063 3196 3563 3948 3965
Size: 116 630 210 135 133 367 385 17
Cuts at: 3965 5417 5503 5515 5861 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 630 385 367 346 210 135
133 116 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2379 3147 513
Size: 552 1314 768 3232
Fragments arranged by size:
3232 1314 768 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2132 4873 809
Size: 507 816 2741 1802
Fragments arranged by size:
2741 1802 816 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2538 2838
Size: 54 1425 50 275 18 552 156 300
Cuts at: 2838 3313 4202 5831 8
Size: 475 889 1629 43
Fragments arranged by size:
1629 1425 889 552 475 300 275 156
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2838 3313 1537
Size: 275 1026 475 4090
Fragments arranged by size:
4090 1026 475 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2570 3796 1353
Size: 761 456 1226 3423
Fragments arranged by size:
3423 1226 761 456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3107 4453 4827 4973 387
Size: 1064 138 1518 1346 374 146 1280
Fragments arranged by size:
1518 1346 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 2708 2929 3929 4526 5313 5581
Size: 393 705 371 221 1000 597 787 268
Cuts at: 5581 1239
Size: 1524
Fragments arranged by size:
1524 1000 787 705 597 393 371 268
221
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 5866
FspI TGC'GCA
Cuts at: 2894 4372 2894
Size: 1478 4388
HaeII r_GCGC'y
Cuts at: 1678 2328 2363 3055 3128 5013 1678
Size: 650 35 692 73 1885 2531
Fragments arranged by size:
2531 1885 692 650 73 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2462 2482 2494 2519
Size: 32 135 32 878 12 20 12 25
Cuts at: 2519 2551 2600 2632 4507 4539 4581 4613 1373
Size: 32 49 32 1875 32 42 32 2626
Fragments arranged by size:
2626 1875 878 135 49 42 32 32
32 32 32 32 25 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2665 3210 3456 669
Size: 988 281 727 545 246 3079
Fragments arranged by size:
3079 988 727 545 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KasI G'GCGC_C
Cuts at: 3124 3124
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 2829 5742 2829
Size: 2913 2953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 2472 2532 4980 5164 1305
Size: 1008 159 60 2448 184 2007
Fragments arranged by size:
2448 2007 1008 184 159 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4044
Fragments arranged by size:
4044 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3824 4183 4342 518
Size: 648 2658 359 159 2042
Fragments arranged by size:
2658 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3282 3959 5107 5352 1606
Size: 1676 677 1148 245 2120
Fragments arranged by size:
2120 1676 1148 677 245
NaeI GCC'GGC
Cuts at: 2329 2547 2329
Size: 218 5648
NarI GG'CG_CC
Cuts at: 3125 3125
Size: 5866
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5314
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NgoMIV G'CCGG_C
Cuts at: 2327 2545 2327
Size: 218 5648
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 2938 3224 4769 1132
Size: 97 1709 286 1545 2229
Fragments arranged by size:
2229 1709 1545 286 97
PasI CC'CwG_GG
Cuts at: 1348 2494 1348
Size: 1146 4720
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 1108
Size: 1539 4327
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2175 1613
Size: 562 5304
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PstI C_TGCA'G
Cuts at: 830 3218 830
Size: 2388 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 2154 3214 4349 5030 5221 826
Size: 219 1109 1060 1135 681 191 1471
Fragments arranged by size:
1471 1135 1109 1060 681 219 191
SfoI GGC'GCC
Cuts at: 3126 3126
Size: 5866
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 2506 2727 3193 1872
Size: 634 221 466 4545
Fragments arranged by size:
4545 634 466 221
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3071 3970 4871 5133 820
Size: 197 926 819 309 899 901 262 1553
Fragments arranged by size:
1553 926 901 899 819 309 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 2938 3224 1132
Size: 1806 286 3774
Fragments arranged by size:
3774 1806 286
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 3305 513
Size: 552 2240 3074
Fragments arranged by size:
3074 2240 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 3893 3910 4063 4248 4693 325
Size: 1255 585 1728 17 153 185 445 1498
Fragments arranged by size:
1728 1498 1255 585 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4112 5824 96
Size: 274 80 33 51 1443 2135 1712 138
Fragments arranged by size:
2135 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2548 2729 2386
Size: 162 181 5523
Fragments arranged by size:
5523 181 162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 2504 2725 3191 1870
Size: 634 221 466 4545
Fragments arranged by size:
4545 634 466 221
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2376 3674 276
Size: 53 83 186 936 842 1298 2468
Fragments arranged by size:
2468 1298 936 842 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI