Restriction map for
pFN21AB8983
(Circular) (Six-base) MAPSORT of: pFN21AB8983.seq Check: 2160 from: 1 to: 5857
pFN21AB8983 5857 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 5304 5572 1632
Size: 2288 1384 268 1917
Fragments arranged by size:
2288 1917 1384 268
AfeI AGC'GCT
Cuts at: 2654 2654
Size: 5857
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 3600 5172 3600
Size: 1572 4285
ApaI G_GGCC'C
Cuts at: 2074 2946 3065 2074
Size: 872 119 4866
Fragments arranged by size:
4866 872 119
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2189 2527 2555 2648 2714 2906
Size: 73 131 115 338 28 93 66 192
Cuts at: 2906 3026 3182 1870
Size: 120 156 4545
Fragments arranged by size:
4545 338 192 156 131 120 115 93
73 66 28
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2615 2710 2833 2858 3178
Size: 325 217 627 828 95 123 25 320
Cuts at: 3178 4633 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 828 627 325 320 217 123
95 25
BanII G_rGCy'C
Cuts at: 1391 2074 2514 2679 2797 2946 3033 3065 3176
Size: 683 440 165 118 149 87 32 111
Cuts at: 3176 1391
Size: 4072
Fragments arranged by size:
4072 683 440 165 149 118 111 87
32
BbeI G_GCGC'C
Cuts at: 2619 2714 2837 2619
Size: 95 123 5639
Fragments arranged by size:
5639 123 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4886
BbvCI CC'TCA_GC
Cuts at: 2210 2210
Size: 5857
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2232 2266 4048 4082 1903
Size: 34 50 34 211 34 1782 34 3678
Fragments arranged by size:
3678 1782 211 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2220 2941 4468
Size: 107 122 71 631 605 548 721 1527
Cuts at: 4468 136
Size: 1525
Fragments arranged by size:
1527 1525 721 631 605 548 122 107
71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 2316 2348 3274 2316
Size: 32 926 4899
Fragments arranged by size:
4899 926 32
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2870 2946 3065 3918 5074 1790
Size: 284 796 76 119 853 1156 2573
Fragments arranged by size:
2573 1156 853 796 284 119 76
BmgBI CAC'GTC
Cuts at: 1318 2968 1318
Size: 1650 4207
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3068 3588 4546
Size: 416 53 83 399 24 1651 520 958
Cuts at: 4546 442
Size: 1753
Fragments arranged by size:
1753 1651 958 520 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2528 2693 2942 4517 1239
Size: 1289 165 249 1575 2579
Fragments arranged by size:
2579 1575 1289 249 165
Bpu10I CC'TnA_GC
Cuts at: 2210 2813 2210
Size: 603 5254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3043 3648 493
Size: 711 1839 605 2702
Fragments arranged by size:
2702 1839 711 605
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2573 2616 2711 2834
Size: 53 83 186 936 1040 43 95 123
Cuts at: 2834 3021 3117 3664 4046 4681 275
Size: 187 96 547 382 635 1451
Fragments arranged by size:
1451 1040 936 635 547 382 187 186
123 96 95 83 53 43
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5632
Fragments arranged by size:
5632 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3101 1186
Size: 1915 3942
BseYI C'CCAG_C
Cuts at: 2159 2359 2611 2938 5060 5556 2159
Size: 200 252 327 2122 496 2460
Fragments arranged by size:
2460 2122 496 327 252 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2453 1163
Size: 573 582 135 4567
Fragments arranged by size:
4567 582 573 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2220 2224 2471 3307 4068 5096
Size: 275 186 219 4 247 836 761 1028
Cuts at: 5096 1540
Size: 2301
Fragments arranged by size:
2301 1028 836 761 275 247 219 186
4
BsiHKAI G_wGCw'C
Cuts at: 2349 3918 4003 5074 2349
Size: 1569 85 1071 3132
Fragments arranged by size:
3132 1569 1071 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2349 2514 2679 2797 2870 2946
Size: 399 284 275 165 165 118 73 76
Cuts at: 2946 3033 3065 3176 3918 4003 5074 1391
Size: 87 32 111 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 284 275 165 165
118 111 87 85 76 73 32
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 3212 844
Size: 1496 872 3489
Fragments arranged by size:
3489 1496 872
BsrBI CCG'CTC
Cuts at: 1394 1823 2839 3744 1394
Size: 429 1016 905 3507
Fragments arranged by size:
3507 1016 905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2114 2219 2466 4501 4688 1700
Size: 110 304 105 247 2035 187 2869
Fragments arranged by size:
2869 2035 304 247 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 2489 2724 2742 3067 1925
Size: 564 235 18 325 4715
Fragments arranged by size:
4715 564 325 235 18
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2440 794
Size: 1646 4211
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 3187 3554 3939 3956 5408 5494 5506 5852
Size: 1215 367 385 17 1452 86 12 346
Cuts at: 5852 1972
Size: 1977
Fragments arranged by size:
1977 1452 1215 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2224 2568 2706 2829 513
Size: 552 1159 344 138 123 3541
Fragments arranged by size:
3541 1159 552 344 138 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 4167 4187 3373
Size: 794 20 5043
Fragments arranged by size:
5043 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2221 2468
Size: 54 1425 50 275 18 387 4 247
Cuts at: 2468 3051 3304 4193 5822 8
Size: 583 253 889 1629 43
Fragments arranged by size:
1629 1425 889 583 387 275 253 247
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2217 2221 2468 3304 1537
Size: 275 405 4 247 836 4090
Fragments arranged by size:
4090 836 405 275 247 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3787 1353
Size: 2434 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2405 2819 4444 4818 4964 387
Size: 1064 138 816 414 1625 374 146 1280
Fragments arranged by size:
1625 1280 1064 816 414 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 2693 2942 3920 4517 5304 5572
Size: 393 896 165 249 978 597 787 268
Cuts at: 5572 1239
Size: 1524
Fragments arranged by size:
1524 978 896 787 597 393 268 249
165
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 2804
Size: 5857
EcoO109I rG'GnC_Cy
Cuts at: 2071 2773 2911 2942 2071
Size: 702 138 31 4986
Fragments arranged by size:
4986 702 138 31
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 5857
FseI GG_CCGG'CC
Cuts at: 2223 2223
Size: 5857
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 2203 2540 2619 2656 2714 2813 2837 2931
Size: 525 337 79 37 58 99 24 94
Cuts at: 2931 5004 1678
Size: 2073 2531
Fragments arranged by size:
2531 2073 525 337 99 94 79 58
37 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4498 4530 4572 4604 1373
Size: 32 135 32 2926 32 42 32 2626
Fragments arranged by size:
2926 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KasI G'GCGC_C
Cuts at: 2615 2710 2833 2615
Size: 95 123 5639
Fragments arranged by size:
5639 123 95
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3093 4971 5155 1305
Size: 1788 1878 184 2007
Fragments arranged by size:
2007 1878 1788 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3053 10
Size: 54 1425 343 1221 2814
Fragments arranged by size:
2814 1425 1221 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3815 4174 4333 518
Size: 648 2649 359 159 2042
Fragments arranged by size:
2649 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2093 2226 2570 2611 2708 2831 2893 2920
Size: 487 133 344 41 97 123 62 27
Cuts at: 2920 2938 3273 3950 5098 5343 1606
Size: 18 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 487 344 335 245 133
123 97 62 41 27 18
NaeI GCC'GGC
Cuts at: 2116 2221 2116
Size: 105 5752
NarI GG'CG_CC
Cuts at: 2616 2711 2834 2616
Size: 95 123 5639
Fragments arranged by size:
5639 123 95
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5305
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NgoMIV G'CCGG_C
Cuts at: 2114 2219 2114
Size: 105 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 3215 4760 1132
Size: 97 1986 1545 2229
Fragments arranged by size:
2229 1986 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2863 1348
Size: 1515 4342
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PmlI CAC'GTG
Cuts at: 3043 3043
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2773 2773
Size: 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspOMI G'GGCC_C
Cuts at: 2070 2942 3061 2070
Size: 872 119 4866
Fragments arranged by size:
4866 872 119
PstI C_TGCA'G
Cuts at: 830 3005 3209 830
Size: 2175 204 3478
Fragments arranged by size:
3478 2175 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
SacII CC_GC'GG
Cuts at: 2227 2571 2709 2832 2227
Size: 344 138 123 5252
Fragments arranged by size:
5252 344 138 123
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SanDI GG'GwC_CC
Cuts at: 2773 2773
Size: 5857
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 3001 3205 4340 5021 5212 826
Size: 219 1956 204 1135 681 191 1471
Fragments arranged by size:
1956 1471 1135 681 219 204 191
SfoI GGC'GCC
Cuts at: 2617 2712 2835 2617
Size: 95 123 5639
Fragments arranged by size:
5639 123 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 2076 2529 3028 3184 1872
Size: 204 453 499 156 4545
Fragments arranged by size:
4545 499 453 204 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 3296 513
Size: 552 2231 3074
Fragments arranged by size:
3074 2231 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3884 3901 4054 4239 4684 325
Size: 1255 2304 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2850 4103 5815
Size: 274 80 33 51 1443 873 1253 1712
Cuts at: 5815 96
Size: 138
Fragments arranged by size:
1712 1443 1253 873 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 2074 2527 3026 3182 1870
Size: 204 453 499 156 4545
Fragments arranged by size:
4545 499 453 204 156
XmnI GAAnn'nnTTC
Cuts at: 2276 3986 2276
Size: 1710 4147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI
NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII
SacI SapI SexAI SfiI SrfI SwaI XcmI