Restriction map for
pFN21AB8982
(Circular) (Six-base) MAPSORT of: pFN21AB8982.seq Check: 9156 from: 1 to: 5839
pFN21AB8982 5839 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3902 5286 5554 1632
Size: 2270 1384 268 1917
Fragments arranged by size:
2270 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 4738 5715 1203
Size: 3535 977 1327
Fragments arranged by size:
3535 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 3000 1166
Size: 1834 4005
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 3582 5154 3582
Size: 1572 4267
ApaI G_GGCC'C
Cuts at: 2208 2208
Size: 5839
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 2635 3148 3374 3526 5635 1493
Size: 1142 513 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 513 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2575 3164 1870
Size: 73 138 494 589 4545
Fragments arranged by size:
4545 589 494 138 73
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2419 2554 2780 2825
Size: 325 217 627 239 393 135 226 45
Cuts at: 2825 3128 3160 4615 618
Size: 303 32 1455 1842
Fragments arranged by size:
1842 1455 627 393 325 303 239 226
217 135 45 32
BanII G_rGCy'C
Cuts at: 1391 2012 2079 2208 2885 2921 2928 3158 1391
Size: 621 67 129 677 36 7 230 4072
Fragments arranged by size:
4072 677 621 230 129 67 36 7
BbeI G_GCGC'C
Cuts at: 2030 2829 2030
Size: 799 5040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2708 928
Size: 971 809 4059
Fragments arranged by size:
4059 971 809
BbvCI CC'TCA_GC
Cuts at: 2136 2987 2136
Size: 851 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3007 3041 3118 3152 4030 4064 1903
Size: 34 1070 34 77 34 878 34 3678
Fragments arranged by size:
3678 1070 878 77 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3731 4941 3731
Size: 1210 4629
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2690 4450 136
Size: 107 122 71 631 605 1018 1760 1525
Fragments arranged by size:
1760 1525 1018 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2448 5834 2448
Size: 3386 2453
BlpI GC'TnA_GC
Cuts at: 3041 3062 3256 3041
Size: 21 194 5624
Fragments arranged by size:
5624 194 21
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2208 2422 2559 2783 3131 3900 5056
Size: 340 78 214 137 224 348 769 1156
Cuts at: 5056 1790
Size: 2573
Fragments arranged by size:
2573 1156 769 348 340 224 214 137
78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2047 2922 3022
Size: 416 53 83 399 24 630 875 100
Cuts at: 3022 3570 4528 442
Size: 548 958 1753
Fragments arranged by size:
1753 958 875 630 548 416 399 100
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4499 1239
Size: 3260 2579
Bpu10I CC'TnA_GC
Cuts at: 2136 2922 2987 2136
Size: 786 65 4988
Fragments arranged by size:
4988 786 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3964 4829 5127 3964
Size: 865 298 4676
Fragments arranged by size:
4676 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4502 882
Size: 617 3003 2219
Fragments arranged by size:
3003 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2461 3630 493
Size: 711 1257 1169 2702
Fragments arranged by size:
2702 1257 1169 711
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2826 3646 4028
Size: 53 83 186 936 494 799 820 382
Cuts at: 4028 4663 275
Size: 635 1451
Fragments arranged by size:
1451 936 820 799 635 494 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2954 2984 3042 3072 1178
Size: 30 165 30 1551 30 58 30 3945
Fragments arranged by size:
3945 1551 165 58 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2366 1186
Size: 1180 4659
BseYI C'CCAG_C
Cuts at: 2060 2129 2294 5042 5538 2060
Size: 69 165 2748 496 2361
Fragments arranged by size:
2748 2361 496 165 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 1163
Size: 573 561 4705
Fragments arranged by size:
4705 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 3063 3900 3985 5056 2012
Size: 1051 837 85 1071 2795
Fragments arranged by size:
2795 1071 1051 837 85
BsmI GAATG_Cn'
Cuts at: 3350 3443 3350
Size: 93 5746
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2079 2130 2208 2422 2559 2783
Size: 399 222 67 51 78 214 137 224
Cuts at: 2783 2885 2921 2928 3063 3131 3158 3900 3985
Size: 102 36 7 135 68 27 742 85
Cuts at: 3985 5056 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 224 222 214 137
135 102 85 78 68 67 51 36
27 7
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 2238 3194 844
Size: 1184 210 956 3489
Fragments arranged by size:
3489 1184 956 210
BsrBI CCG'CTC
Cuts at: 1394 1823 2874 2977 3726 1394
Size: 429 1051 103 749 3507
Fragments arranged by size:
3507 1051 749 429 103
BsrDI GCAATG_nn'
Cuts at: 65 1248 4334 4508 65
Size: 1183 3086 174 1396
Fragments arranged by size:
3086 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 2498 4483 4670 1700
Size: 110 213 475 1985 187 2869
Fragments arranged by size:
2869 1985 475 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 2887 3899 4911 2887
Size: 1012 1012 3815
Fragments arranged by size:
3815 1012 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5839
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstXI CCAn_nnnn'nTGG
Cuts at: 2270 3002 2270
Size: 732 5107
BstYI r'GATC_y
Cuts at: 1972 2448 3053 3169 3536 3921 3938 5390 5476
Size: 476 605 116 367 385 17 1452 86
Cuts at: 5476 5488 5834 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 605 476 385 367 346 116
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2748 2748
Size: 5839
BtgI C'CryG_G
Cuts at: 513 1065 2403 2413 2739 513
Size: 552 1338 10 326 3613
Fragments arranged by size:
3613 1338 552 326 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2383 487
Size: 683 56 1157 3943
Fragments arranged by size:
3943 1157 683 56
BtsI GCAGTG_nn'
Cuts at: 3355 4149 4169 3355
Size: 794 20 5025
Fragments arranged by size:
5025 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3085 3120 529
Size: 35 2521 35 3248
Fragments arranged by size:
3248 2521 35 35
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2372 2406
Size: 54 1425 50 275 18 238 304 34
Cuts at: 2406 2463 3286 4175 5804 8
Size: 57 823 889 1629 43
Fragments arranged by size:
1629 1425 889 823 304 275 238 57
54 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2648 3769 1353
Size: 1295 1121 3423
Fragments arranged by size:
3423 1295 1121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2507 4426 4800 4946 387
Size: 1064 138 918 1919 374 146 1280
Fragments arranged by size:
1919 1280 1064 918 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3902 4499 5286 5554 1239
Size: 393 2270 597 787 268 1524
Fragments arranged by size:
2270 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 2261
Size: 5839
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 2030 2434 2829 4986 1678
Size: 352 404 395 2157 2531
Fragments arranged by size:
2531 2157 404 395 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2657 2689 2753 2785 3045
Size: 32 135 32 1085 32 64 32 260
Cuts at: 3045 3077 4480 4512 4554 4586 1373
Size: 32 1403 32 42 32 2626
Fragments arranged by size:
2626 1403 1085 260 135 64 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3097 3183 3429 669
Size: 988 281 1159 86 246 3079
Fragments arranged by size:
3079 1159 988 281 246 86
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KasI G'GCGC_C
Cuts at: 2026 2825 2026
Size: 799 5040
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4953 5137 1305
Size: 3648 184 2007
Fragments arranged by size:
3648 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 10
Size: 54 1425 343 576 3441
Fragments arranged by size:
3441 1425 576 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 3000 3035 3797 4156 4315 518
Size: 648 1236 598 35 762 359 159 2042
Fragments arranged by size:
2042 1236 762 648 598 359 159 35
MspA1I CmG'CkG
Cuts at: 1606 2140 2287 3000 3255 3932 5080 5325 1606
Size: 534 147 713 255 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 677 534 255 245 147
NaeI GCC'GGC
Cuts at: 2025 2500 2025
Size: 475 5364
NarI GG'CG_CC
Cuts at: 2027 2826 2027
Size: 799 5040
NcoI C'CATG_G
Cuts at: 513 1065 2403 513
Size: 552 1338 3949
Fragments arranged by size:
3949 1338 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NgoMIV G'CCGG_C
Cuts at: 2023 2498 2023
Size: 475 5364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 3040 3197 4742 1132
Size: 97 1811 157 1545 2229
Fragments arranged by size:
2229 1811 1545 157 97
PasI CC'CwG_GG
Cuts at: 1348 3120 1348
Size: 1772 4067
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2461 2899 1108
Size: 1353 438 4048
Fragments arranged by size:
4048 1353 438
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 3908 3940 5421 5453 2606
Size: 32 1270 32 1481 32 2992
Fragments arranged by size:
2992 1481 1270 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PspOMI G'GGCC_C
Cuts at: 2204 2204
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2048 3191 830
Size: 1218 1143 3478
Fragments arranged by size:
3478 1218 1143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SfcI C'TryA_G
Cuts at: 826 1045 2044 3187 4322 5003 5194 826
Size: 219 999 1143 1135 681 191 1471
Fragments arranged by size:
1471 1143 1135 999 681 219 191
SfoI GGC'GCC
Cuts at: 2028 2827 2028
Size: 799 5040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 2577 3166 1872
Size: 705 589 4545
Fragments arranged by size:
4545 705 589
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3943 4844 5106 820
Size: 197 926 2000 901 262 1553
Fragments arranged by size:
2000 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 1517
Size: 750 5089
StyI C'CwwG_G
Cuts at: 513 1065 2403 2688 2898 3278 513
Size: 552 1338 285 210 380 3074
Fragments arranged by size:
3074 1338 552 380 285 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2130 3866 3883 4036 4221 4666 325
Size: 1255 550 1736 17 153 185 445 1498
Fragments arranged by size:
1736 1498 1255 550 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4085 5797 96
Size: 274 80 33 51 1443 2108 1712 138
Fragments arranged by size:
2108 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3042 3074 5799 5831 3042
Size: 32 2725 32 3050
Fragments arranged by size:
3050 2725 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 2575 3164 1870
Size: 705 589 4545
Fragments arranged by size:
4545 705 589
XmnI GAAnn'nnTTC
Cuts at: 2108 2621 3968 2108
Size: 513 1347 3979
Fragments arranged by size:
3979 1347 513
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI