Restriction map for
pFN21AB8979
(Circular) (Six-base) MAPSORT of: pFN21AB8979.seq Check: 3496 from: 1 to: 5827
pFN21AB8979 5827 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2744 2744
Size: 5827
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 2262 3148 2262
Size: 886 4941
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2238 2350 2717 3890 5274 5542 1632
Size: 606 112 367 1173 1384 268 1917
Fragments arranged by size:
1917 1384 1173 606 367 268 112
AflII C'TTAA_G
Cuts at: 820 1017 2278 820
Size: 197 1261 4369
Fragments arranged by size:
4369 1261 197
AflIII A'CryG_T
Cuts at: 1203 2532 2642 4726 5703 1203
Size: 1329 110 2084 977 1327
Fragments arranged by size:
2084 1329 1327 977 110
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2646 1184
Size: 1462 4365
AlwNI CAG_nnn'CTG
Cuts at: 2030 3570 5142 2030
Size: 1540 1572 2715
Fragments arranged by size:
2715 1572 1540
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2140 3136 3362 3514 5623 1493
Size: 647 996 226 152 2109 1697
Fragments arranged by size:
2109 1697 996 647 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 3152 1870
Size: 73 1209 4545
Fragments arranged by size:
4545 1209 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 3148 4603 618
Size: 325 217 627 475 886 1455 1842
Fragments arranged by size:
1842 1455 886 627 475 325 217
BanII G_rGCy'C
Cuts at: 1391 2134 3146 1391
Size: 743 1012 4072
Fragments arranged by size:
4072 1012 743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BfrBI ATG'CAT
Cuts at: 2876 2876
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2216 3888 5044 1790
Size: 426 1672 1156 2573
Fragments arranged by size:
2573 1672 1156 426
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2488 3558 4516
Size: 416 53 83 399 24 1071 1070 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1071 1070 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4487 1239
Size: 3248 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3634 4016 4651 275
Size: 53 83 186 936 2101 382 635 1451
Fragments arranged by size:
2101 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2862 4260 4658 4932 5079 5490 2862
Size: 1398 398 274 147 411 3199
Fragments arranged by size:
3199 1398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2780 2810 1178
Size: 30 165 30 1377 30 4195
Fragments arranged by size:
4195 1377 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2906 1186
Size: 1720 4107
BseYI C'CCAG_C
Cuts at: 5030 5526 5030
Size: 496 5331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2134 3888 3973 5044 2134
Size: 1754 85 1071 2917
Fragments arranged by size:
2917 1754 1071 85
BsmI GAATG_Cn'
Cuts at: 2804 3338 3431 2804
Size: 534 93 5200
Fragments arranged by size:
5200 534 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 2216 3146 3888 3973 5044 1391
Size: 399 344 82 930 742 85 1071 2174
Fragments arranged by size:
2174 1071 930 742 399 344 85 82
BspEI T'CCGG_A
Cuts at: 2862 5490 2862
Size: 2628 3199
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2305 2744 3182 844
Size: 1461 439 438 3489
Fragments arranged by size:
3489 1461 439 438
BsrBI CCG'CTC
Cuts at: 1394 1823 3714 1394
Size: 429 1891 3507
Fragments arranged by size:
3507 1891 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 2438 4322 4496 65
Size: 1183 1164 26 1884 174 1396
Fragments arranged by size:
1884 1396 1183 1164 174 26
BsrFI r'CCGG_y
Cuts at: 1700 1810 4471 4658 1700
Size: 110 2661 187 2869
Fragments arranged by size:
2869 2661 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 2375 3064 3157 3524 3909 3926 5378 5464
Size: 403 689 93 367 385 17 1452 86
Cuts at: 5464 5476 5822 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 689 403 385 367 346 93
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2256 513
Size: 552 1191 4084
Fragments arranged by size:
4084 1191 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 3108 3343 4137 4157 3108
Size: 235 794 20 4778
Fragments arranged by size:
4778 794 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 2409 2583
Size: 54 1425 50 275 18 504 75 174
Cuts at: 2583 3274 4163 5792 8
Size: 691 889 1629 43
Fragments arranged by size:
1629 1425 889 691 504 275 174 75
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 3757 1353
Size: 792 1612 3423
Fragments arranged by size:
3423 1612 792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2132 2132
Size: 5827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2238 2350 2717 3890 4487 5274 5542
Size: 393 606 112 367 1173 597 787 268
Cuts at: 5542 1239
Size: 1524
Fragments arranged by size:
1524 1173 787 606 597 393 367 268
112
EcoO109I rG'GnC_Cy
Cuts at: 2494 2611 2494
Size: 117 5710
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 5827
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 4974 1678
Size: 3296 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2475 2753 2785 4468
Size: 32 135 32 871 32 278 32 1683
Cuts at: 4468 4500 4542 4574 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1683 871 278 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KpnI G_GTAC'C
Cuts at: 2266 3152 2266
Size: 886 4941
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2803 4941 5125 1305
Size: 1498 2138 184 2007
Fragments arranged by size:
2138 2007 1498 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 2585 10
Size: 54 1425 343 579 174 3252
Fragments arranged by size:
3252 1425 579 343 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2466 2628 2924 3785 4144 4303
Size: 648 1072 228 162 296 861 359 159
Cuts at: 4303 518
Size: 2042
Fragments arranged by size:
2042 1072 861 648 359 296 228 162
159
MspA1I CmG'CkG
Cuts at: 1606 2027 3243 3920 5068 5313 1606
Size: 421 1216 677 1148 245 2120
Fragments arranged by size:
2120 1216 1148 677 421 245
NcoI C'CATG_G
Cuts at: 513 1065 2256 513
Size: 552 1191 4084
Fragments arranged by size:
4084 1191 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NsiI A_TGCA'T
Cuts at: 2878 2878
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 2536 2646 3185 4730 1132
Size: 97 1307 110 539 1545 2229
Fragments arranged by size:
2229 1545 1307 539 110 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 2532 2642 4726 2532
Size: 110 2084 3633
Fragments arranged by size:
3633 2084 110
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2494 2494
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PstI C_TGCA'G
Cuts at: 830 3179 830
Size: 2349 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2027 2027
Size: 5827
SacI G_AGCT'C
Cuts at: 2134 2134
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 2284 4075 5787 2284
Size: 1791 1712 2324
Fragments arranged by size:
2324 1791 1712
SfcI C'TryA_G
Cuts at: 826 1045 3175 4310 4991 5182 826
Size: 219 2130 1135 681 191 1471
Fragments arranged by size:
2130 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2278 3931 4832 5094 820
Size: 197 926 335 1653 901 262 1553
Fragments arranged by size:
1653 1553 926 901 335 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 2708 2959 3751 5
Size: 47 2656 251 792 2081
Fragments arranged by size:
2656 2081 792 251 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 2169 2256 2634 2799 3266 513
Size: 552 1104 87 378 165 467 3074
Fragments arranged by size:
3074 1104 552 467 378 165 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 3871 4024 4209 4654 325
Size: 1255 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2282 2535 4073
Size: 274 80 33 51 1443 305 253 1538
Cuts at: 4073 5785 96
Size: 1712 138
Fragments arranged by size:
1712 1538 1443 305 274 253 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5787 5819 5787
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 2308
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI