Restriction map for
pFN21AB8974
(Circular) (Six-base) MAPSORT of: pFN21AB8974.seq Check: 4595 from: 1 to: 5812
pFN21AB8974 5812 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2220 3155 1937
Size: 283 935 4594
Fragments arranged by size:
4594 935 283
Acc65I G'GTAC_C
Cuts at: 3133 3133
Size: 5812
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2654 2675 3020 3875 5259 5527 1632
Size: 1022 21 345 855 1384 268 1917
Fragments arranged by size:
1917 1384 1022 855 345 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 2256 4711 5688 1203
Size: 1053 2455 977 1327
Fragments arranged by size:
2455 1327 1053 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 2237 1166
Size: 1071 4741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 3555 5127 3555
Size: 1572 4240
ApaLI G'TGCA_C
Cuts at: 3869 5025 3869
Size: 1156 4656
ApoI r'AATT_y
Cuts at: 1493 2269 3121 3347 3499 5608 1493
Size: 776 852 226 152 2109 1697
Fragments arranged by size:
2109 1697 852 776 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 3137 1870
Size: 73 1194 4545
Fragments arranged by size:
4545 1194 73
BamHI G'GATC_C
Cuts at: 3142 3509 3142
Size: 367 5445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2189 3133 4588 618
Size: 325 217 627 402 944 1455 1842
Fragments arranged by size:
1842 1455 944 627 402 325 217
BanII G_rGCy'C
Cuts at: 1391 3131 1391
Size: 1740 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2249 2736 928
Size: 971 189 161 487 4004
Fragments arranged by size:
4004 971 487 189 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 2412 1548
Size: 864 4948
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 3229 3229
Size: 5812
Bme1580I G_kGCm'C
Cuts at: 1790 3873 5029 1790
Size: 2083 1156 2573
Fragments arranged by size:
2573 2083 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3022 3543 4501
Size: 416 53 83 399 24 1605 521 958
Cuts at: 4501 442
Size: 1753
Fragments arranged by size:
1753 1605 958 521 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3104 4472 1239
Size: 1865 1368 2579
Fragments arranged by size:
2579 1865 1368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4475 882
Size: 617 2976 2219
Fragments arranged by size:
2976 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3603 493
Size: 711 2399 2702
Fragments arranged by size:
2702 2399 711
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2844 3619 4001 4636
Size: 53 83 186 936 1311 775 382 635
Cuts at: 4636 275
Size: 1451
Fragments arranged by size:
1451 1311 936 775 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4245 4643 4917 5064 5475 4245
Size: 398 274 147 411 4582
Fragments arranged by size:
4582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5587
Fragments arranged by size:
5587 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 1186
Size: 1093 4719
BseYI C'CCAG_C
Cuts at: 5015 5511 5015
Size: 496 5316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2215 2581 2852 1163
Size: 573 479 366 271 4123
Fragments arranged by size:
4123 573 479 366 271
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2467 2627 3103 3873 3958 5029 2467
Size: 160 476 770 85 1071 3250
Fragments arranged by size:
3250 1071 770 476 160 85
BsmI GAATG_Cn'
Cuts at: 3323 3416 3323
Size: 93 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2467 2627 3103 3131 3873 3958 5029
Size: 399 677 160 476 28 742 85 1071
Cuts at: 5029 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 677 476 399 160 85
28
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 3701 5431 3701
Size: 1730 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3167 844
Size: 2323 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3699 1394
Size: 429 1876 3507
Fragments arranged by size:
3507 1876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 2439 2445 2586 4307 4481 65
Size: 1183 1071 120 6 141 1721 174 1396
Fragments arranged by size:
1721 1396 1183 1071 174 141 120 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 2379 2493 96
Size: 2283 114 3415
Fragments arranged by size:
3415 2283 114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 2174 2987 3872 4884 2174
Size: 813 885 1012 3102
Fragments arranged by size:
3102 1012 885 813
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5812
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 2905 1184
Size: 1721 4091
BstYI r'GATC_y
Cuts at: 1972 2983 3142 3509 3894 3911 5363 5449 5461
Size: 1011 159 367 385 17 1452 86 12
Cuts at: 5461 5807 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 159 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3094 3094
Size: 5812
BtgI C'CryG_G
Cuts at: 513 1065 2076 2601 513
Size: 552 1011 525 3724
Fragments arranged by size:
3724 1011 552 525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2631 2902 487
Size: 683 56 1405 271 3397
Fragments arranged by size:
3397 1405 683 271 56
BtsI GCAGTG_nn'
Cuts at: 3328 4122 4142 3328
Size: 794 20 4998
Fragments arranged by size:
4998 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2869 2904 529
Size: 35 2305 35 3437
Fragments arranged by size:
3437 2305 35 35
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4819 809
Size: 507 3503 1802
Fragments arranged by size:
3503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3259 4148 5777
Size: 54 1425 50 275 18 1429 889 1629
Cuts at: 5777 8
Size: 43
Fragments arranged by size:
1629 1429 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 3742 1353
Size: 957 1432 3423
Fragments arranged by size:
3423 1432 957
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2966 4399 4773 4919 387
Size: 1064 138 1377 1433 374 146 1280
Fragments arranged by size:
1433 1377 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2654 2675 3020 3104 3875 4472 5259
Size: 393 1022 21 345 84 771 597 787
Cuts at: 5259 5527 1239
Size: 268 1524
Fragments arranged by size:
1524 1022 787 771 597 393 345 268
84 21
EcoRI G'AATT_C
Cuts at: 3121 3499 3121
Size: 378 5434
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 5812
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 4959 1678
Size: 3281 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 4453 4485 4527
Size: 32 135 32 470 32 2379 32 42
Cuts at: 4527 4559 1373
Size: 32 2626
Fragments arranged by size:
2626 2379 470 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2092 2371 3156 3402 669
Size: 988 281 154 279 785 246 3079
Fragments arranged by size:
3079 988 785 281 279 246 154
HindIII A'AGCT_T
Cuts at: 748 3058 748
Size: 2310 3502
HpaI GTT'AAC
Cuts at: 2092 3402 2092
Size: 1310 4502
KpnI G_GTAC'C
Cuts at: 3137 3137
Size: 5812
MfeI C'AATT_G
Cuts at: 2277 3411 2277
Size: 1134 4678
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2889 4926 5110 1305
Size: 1584 2037 184 2007
Fragments arranged by size:
2037 2007 1584 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3990
Fragments arranged by size:
3990 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2426 3770 4129 4288 518
Size: 648 1071 189 1344 359 159 2042
Fragments arranged by size:
2042 1344 1071 648 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2400 2534 2642 3228 3905 5053 5298 1606
Size: 794 134 108 586 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 586 245 134 108
NcoI C'CATG_G
Cuts at: 513 1065 2076 2601 513
Size: 552 1011 525 3724
Fragments arranged by size:
3724 1011 552 525
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2260 2380 3170 4715 1132
Size: 97 1031 120 790 1545 2229
Fragments arranged by size:
2229 1545 1031 790 120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5812
PciI A'CATG_T
Cuts at: 2256 4711 2256
Size: 2455 3357
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PstI C_TGCA'G
Cuts at: 830 3164 830
Size: 2334 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
PvuII CAG'CTG
Cuts at: 2400 2534 2642 2400
Size: 134 108 5570
Fragments arranged by size:
5570 134 108
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 2395 3160 4295 4976 5167 826
Size: 219 1350 765 1135 681 191 1471
Fragments arranged by size:
1471 1350 1135 765 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 3139 1872
Size: 1267 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 4817 5079 820
Size: 197 926 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 2076 2193 2601 3251 513
Size: 552 1011 117 408 650 3074
Fragments arranged by size:
3074 1011 650 552 408 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2177 2267 2924 2954 3839 3856
Size: 1255 446 151 90 657 30 885 17
Cuts at: 3856 4009 4194 4639 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 885 657 446 445 185 153
151 90 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2379 2493
Size: 274 80 33 51 1443 189 213 114
Cuts at: 2493 4058 5770 96
Size: 1565 1712 138
Fragments arranged by size:
1712 1565 1443 274 213 189 138 114
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5772 5804 5772
Size: 32 5780
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2166 2524 2074
Size: 92 358 5362
Fragments arranged by size:
5362 358 92
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 3137 1870
Size: 1267 4545
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI