Restriction map for
pFN21AB8973
(Circular) (Six-base) MAPSORT of: pFN21AB8973.seq Check: 6605 from: 1 to: 5806
pFN21AB8973 5806 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2465 2726 2465
Size: 261 5545
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2355 2859 3149 1937
Size: 418 504 290 4594
Fragments arranged by size:
4594 504 418 290
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3869 5253 5521 1632
Size: 2237 1384 268 1917
Fragments arranged by size:
2237 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 2289 2430 4705 5682 1203
Size: 1086 141 2275 977 1327
Fragments arranged by size:
2275 1327 1086 977 141
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 2269 4535 2269
Size: 2266 3540
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 2072 2755 3549 5121 2072
Size: 683 794 1572 2757
Fragments arranged by size:
2757 1572 794 683
ApaI G_GGCC'C
Cuts at: 3047 3047
Size: 5806
ApaLI G'TGCA_C
Cuts at: 2489 3863 5019 2489
Size: 1374 1156 3276
Fragments arranged by size:
3276 1374 1156
ApoI r'AATT_y
Cuts at: 1493 3115 3341 3493 5602 1493
Size: 1622 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 3131 1870
Size: 73 1188 4545
Fragments arranged by size:
4545 1188 73
BamHI G'GATC_C
Cuts at: 2030 3136 3503 2030
Size: 1106 367 4333
Fragments arranged by size:
4333 1106 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3127 4582 618
Size: 325 217 627 1340 1455 1842
Fragments arranged by size:
1842 1455 1340 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2597 3047 3083 3125 1391
Size: 1206 450 36 42 4072
Fragments arranged by size:
4072 1206 450 42 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 2121 2730 1548
Size: 573 609 4624
Fragments arranged by size:
4624 609 573
BfrBI ATG'CAT
Cuts at: 2302 2302
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4417 136
Size: 107 122 71 631 605 2745 1525
Fragments arranged by size:
2745 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 2493 2838 2962 3047 3867 5023 1790
Size: 703 345 124 85 820 1156 2573
Fragments arranged by size:
2573 1156 820 703 345 124 85
BmgBI CAC'GTC
Cuts at: 1318 2845 1318
Size: 1527 4279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2195 4466 1239
Size: 823 133 2271 2579
Fragments arranged by size:
2579 2271 823 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2759 3023 3931 4796 5094 2492
Size: 267 264 908 865 298 3204
Fragments arranged by size:
3204 908 865 298 267 264
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2256 4469 882
Size: 617 757 2213 2219
Fragments arranged by size:
2219 2213 757 617
BsaAI yAC'GTr
Cuts at: 493 1204 2863 3597 493
Size: 711 1659 734 2702
Fragments arranged by size:
2702 1659 734 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2168 3613 3995 4630
Size: 53 83 186 936 635 1445 382 635
Cuts at: 4630 275
Size: 1451
Fragments arranged by size:
1451 1445 936 635 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4239 4637 4911 5058 5469 4239
Size: 398 274 147 411 4576
Fragments arranged by size:
4576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5806
BseYI C'CCAG_C
Cuts at: 2901 3053 5009 5505 2901
Size: 152 1956 496 3202
Fragments arranged by size:
3202 1956 496 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 2852 1163
Size: 573 891 225 4117
Fragments arranged by size:
4117 891 573 225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2473 3256 4017 5045 1540
Size: 275 186 472 783 761 1028 2301
Fragments arranged by size:
2301 1028 783 761 472 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 3083 3867 3952 5023 2493
Size: 590 784 85 1071 3276
Fragments arranged by size:
3276 1071 784 590 85
BsmI GAATG_Cn'
Cuts at: 3317 3410 3317
Size: 93 5713
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2408 2159
Size: 249 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 2597 2838 2962 3047 3083 3125
Size: 399 703 104 241 124 85 36 42
Cuts at: 3125 3867 3952 5023 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 703 399 241 124 104
85 85 42 36
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2465 2726 2733 3161 844
Size: 1621 261 7 428 3489
Fragments arranged by size:
3489 1621 428 261 7
BsrBI CCG'CTC
Cuts at: 1394 1823 2853 3693 1394
Size: 429 1030 840 3507
Fragments arranged by size:
3507 1030 840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2159 4301 4475 65
Size: 1183 911 2142 174 1396
Fragments arranged by size:
2142 1396 1183 911 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2408 4450 4637 1700
Size: 110 598 2042 187 2869
Fragments arranged by size:
2869 2042 598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2755 794
Size: 1961 3845
BstBI TT'CG_AA
Cuts at: 3060 4610 3060
Size: 1550 4256
BstEII G'GTnAC_C
Cuts at: 1184 2713 1184
Size: 1529 4277
BstXI CCAn_nnnn'nTGG
Cuts at: 2350 2366 2809 2821 2350
Size: 16 443 12 5335
Fragments arranged by size:
5335 443 16 12
BstYI r'GATC_y
Cuts at: 1972 2030 3136 3503 3888 3905 5357 5443 5455
Size: 58 1106 367 385 17 1452 86 12
Cuts at: 5455 5801 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1106 385 367 346 86 58
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2142 2310 2574 2709 2881 513
Size: 552 1077 168 264 135 172 3438
Fragments arranged by size:
3438 1077 552 264 172 168 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 2688 2793 3322 4116 4136 2688
Size: 105 529 794 20 4358
Fragments arranged by size:
4358 794 529 105 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DraIII CAC_nnn'GTG
Cuts at: 2623 2623
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2158 2283
Size: 54 1425 50 275 18 309 19 125
Cuts at: 2283 2307 2313 2378 2481 2571 2811 3253 4142
Size: 24 6 65 103 90 240 442 889
Cuts at: 4142 5771 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 442 309 275 240 125
103 90 65 54 50 43 24 19
18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 3736 1353
Size: 1070 1313 3423
Fragments arranged by size:
3423 1313 1070
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2990 4393 4767 4913 387
Size: 1064 138 1401 1403 374 146 1280
Fragments arranged by size:
1403 1401 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3081 3081
Size: 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2195 3869 4466 5253 5521 1239
Size: 393 430 133 1674 597 787 268 1524
Fragments arranged by size:
1674 1524 787 597 430 393 268 133
EcoNI CCTnn'n_nnAGG
Cuts at: 2747 2747
Size: 5806
EcoO109I rG'GnC_Cy
Cuts at: 2277 2520 2752 2886 3043 2277
Size: 243 232 134 157 5040
Fragments arranged by size:
5040 243 232 157 134
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 2792 4312 2792
Size: 1520 4286
FspAI rTGC'GCAy
Cuts at: 2792 2792
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2469 2773 4953 1678
Size: 791 304 2180 2531
Fragments arranged by size:
2531 2180 791 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 4447 4479 4521
Size: 32 135 32 683 32 2160 32 42
Cuts at: 4521 4553 1373
Size: 32 2626
Fragments arranged by size:
2626 2160 683 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2848 3150 3396 669
Size: 988 281 910 302 246 3079
Fragments arranged by size:
3079 988 910 302 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4920 5104 1305
Size: 3615 184 2007
Fragments arranged by size:
3615 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 2160 2380 2483 2573
Size: 54 1425 343 309 19 220 103 90
Cuts at: 2573 2813 10
Size: 240 3003
Fragments arranged by size:
3003 1425 343 309 240 220 103 90
54 19
MslI CAynn'nnrTG
Cuts at: 518 1166 3764 4123 4282 518
Size: 648 2598 359 159 2042
Fragments arranged by size:
2598 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2584 2638 3222 3899 5047 5292 1606
Size: 978 54 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 978 677 584 245 54
NcoI C'CATG_G
Cuts at: 513 1065 2574 2881 513
Size: 552 1509 307 3438
Fragments arranged by size:
3438 1509 552 307
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NsiI A_TGCA'T
Cuts at: 2304 2304
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2185 2400 2434 3164 4709 1132
Size: 97 956 215 34 730 1545 2229
Fragments arranged by size:
2229 1545 956 730 215 97 34
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 2430 4705 2430
Size: 2275 3531
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 2668 1108
Size: 1515 45 4246
Fragments arranged by size:
4246 1515 45
PfoI T'CCnGG_A
Cuts at: 2273 2273
Size: 5806
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2277 2277
Size: 5806
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PspOMI G'GGCC_C
Cuts at: 3043 3043
Size: 5806
PstI C_TGCA'G
Cuts at: 830 2637 3158 830
Size: 1807 521 3478
Fragments arranged by size:
3478 1807 521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 2638 2638
Size: 5806
SacI G_AGCT'C
Cuts at: 3083 3083
Size: 5806
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SanDI GG'GwC_CC
Cuts at: 2277 2277
Size: 5806
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 2633 3154 4289 4970 5161 826
Size: 219 1588 521 1135 681 191 1471
Fragments arranged by size:
1588 1471 1135 681 521 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 3133 1872
Size: 1261 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2507 2774 3038 3910 4811 5073
Size: 197 926 564 267 264 872 901 262
Cuts at: 5073 820
Size: 1553
Fragments arranged by size:
1553 926 901 872 564 267 264 262
197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 2400 3164 1132
Size: 1268 764 3774
Fragments arranged by size:
3774 1268 764
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 2654 1517
Size: 1137 4669
StyI C'CwwG_G
Cuts at: 513 1065 2574 2781 2881 3245 513
Size: 552 1509 207 100 364 3074
Fragments arranged by size:
3074 1509 552 364 207 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2545 2915 3833 3850 4003 4188 4633
Size: 1255 965 370 918 17 153 185 445
Cuts at: 4633 325
Size: 1498
Fragments arranged by size:
1498 1255 965 918 445 370 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5766 5798 5766
Size: 32 5774
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 2134 2381 2620 3100 2090
Size: 44 247 239 480 4796
Fragments arranged by size:
4796 480 247 239 44
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 3131 1870
Size: 1261 4545
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BstZ17I Bsu36I EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI