Restriction map for
pFN21AB8970
(Circular) (Six-base) MAPSORT of: pFN21AB8970.seq Check: 2686 from: 1 to: 5782
pFN21AB8970 5782 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 2925 3845 5229 5497 1632
Size: 436 857 920 1384 268 1917
Fragments arranged by size:
1917 1384 920 857 436 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2042 2389 1166
Size: 876 347 4559
Fragments arranged by size:
4559 876 347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2407 1184
Size: 1223 4559
AlwNI CAG_nnn'CTG
Cuts at: 2730 2776 3525 5097 2730
Size: 46 749 1572 3415
Fragments arranged by size:
3415 1572 749 46
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 3107 1870
Size: 73 191 973 4545
Fragments arranged by size:
4545 973 191 73
AvrII C'CTAG_G
Cuts at: 2404 2404
Size: 5782
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2409 2543 2663 2706 3103
Size: 325 217 627 622 134 120 43 397
Cuts at: 3103 4558 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 622 397 325 217 134
120 43
BanII G_rGCy'C
Cuts at: 1391 2355 2582 3101 1391
Size: 964 227 519 4072
Fragments arranged by size:
4072 964 519 227
BbeI G_GCGC'C
Cuts at: 2667 2667
Size: 5782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2176 2922 928
Size: 971 277 746 3788
Fragments arranged by size:
3788 971 746 277
BbvCI CC'TCA_GC
Cuts at: 2769 2769
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 4393 136
Size: 107 122 71 631 605 428 2293 1525
Fragments arranged by size:
2293 1525 631 605 428 122 107 71
BglII A'GATC_T
Cuts at: 2984 5777 2984
Size: 2793 2989
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2412 3843 4999 1790
Size: 622 1431 1156 2573
Fragments arranged by size:
2573 1431 1156 622
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2599 2798 2973 4442 1239
Size: 1169 191 199 175 1469 2579
Fragments arranged by size:
2579 1469 1169 199 191 175
Bpu10I CC'TnA_GC
Cuts at: 2240 2769 2240
Size: 529 5253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 2938 2972 5776 2938
Size: 34 2804 2944
Fragments arranged by size:
2944 2804 34
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2158 2664 3589 3971
Size: 53 83 186 936 625 506 925 382
Cuts at: 3971 4606 275
Size: 635 1451
Fragments arranged by size:
1451 936 925 635 625 506 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2274 4215 4613 4887 5034 5445 2274
Size: 1941 398 274 147 411 2611
Fragments arranged by size:
2611 1941 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2355 2385 2699 2729 1178
Size: 30 165 30 952 30 314 30 4231
Fragments arranged by size:
4231 952 314 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2347 2588 2795 1186
Size: 1161 241 207 4173
Fragments arranged by size:
4173 1161 241 207
BseYI C'CCAG_C
Cuts at: 2140 2224 2593 4985 5481 2140
Size: 84 369 2392 496 2441
Fragments arranged by size:
2441 2392 496 369 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2549 2704 3070 1163
Size: 573 813 155 366 3875
Fragments arranged by size:
3875 813 573 366 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2153 2610 2823 3843 3928 4999 2082
Size: 71 457 213 1020 85 1071 2865
Fragments arranged by size:
2865 1071 1020 457 213 85 71
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2153 2355 2412 2582 2610 2823
Size: 399 292 71 202 57 170 28 213
Cuts at: 2823 3101 3843 3928 4999 1391
Size: 278 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 292 278 213 202
170 85 71 57 28
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 3137 844
Size: 1271 1022 3489
Fragments arranged by size:
3489 1271 1022
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 2691 3045 4426 4613 1700
Size: 110 280 601 354 1381 187 2869
Fragments arranged by size:
2869 1381 601 354 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 2129 2496 2743 1925
Size: 204 367 247 4964
Fragments arranged by size:
4964 367 247 204
BssSI C'ACGA_G
Cuts at: 2570 3842 4854 2570
Size: 1272 1012 3498
Fragments arranged by size:
3498 1272 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2550 794
Size: 1756 4026
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 2212 1184
Size: 1028 4754
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 2386 2623 3067 2044
Size: 342 237 444 4759
Fragments arranged by size:
4759 444 342 237
BstYI r'GATC_y
Cuts at: 1972 2984 3112 3479 3864 3881 5333 5419 5431
Size: 1012 128 367 385 17 1452 86 12
Cuts at: 5431 5777 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1012 385 367 346 128 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2379 513
Size: 552 1314 3916
Fragments arranged by size:
3916 1314 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2699 487
Size: 683 56 1473 3570
Fragments arranged by size:
3570 1473 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 2363 3298 4092 4112 2162
Size: 201 935 794 20 3832
Fragments arranged by size:
3832 935 794 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DraIII CAC_nnn'GTG
Cuts at: 2550 2832 2550
Size: 282 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3229 4118
Size: 54 1425 50 275 18 546 853 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 853 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2828 3712 1353
Size: 734 741 884 3423
Fragments arranged by size:
3423 884 741 734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2408 2599 2798 2925 2973 3845
Size: 393 436 340 191 199 127 48 872
Cuts at: 3845 4442 5229 5497 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 872 787 597 436 393 340 268
199 191 127 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2722 2722
Size: 5782
EcoO109I rG'GnC_Cy
Cuts at: 2898 2898
Size: 5782
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 2672 4288 2672
Size: 1616 4166
HaeII r_GCGC'y
Cuts at: 1678 2478 2630 2667 2810 4929 1678
Size: 800 152 37 143 2119 2531
Fragments arranged by size:
2531 2119 800 152 143 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2418 2450 4423 4455 4497
Size: 32 135 32 846 32 1973 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1973 846 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KasI G'GCGC_C
Cuts at: 2663 2663
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 2952 3381 2952
Size: 429 5353
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3414
Fragments arranged by size:
3414 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2195 2389 3065 3740 4099 4258
Size: 648 876 153 194 676 675 359 159
Cuts at: 4258 518
Size: 2042
Fragments arranged by size:
2042 876 676 675 648 359 194 159
153
MspA1I CmG'CkG
Cuts at: 1606 2025 2173 2704 3198 3875 5023 5268 1606
Size: 419 148 531 494 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 494 419 245 148
NarI GG'CG_CC
Cuts at: 2664 2664
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 2379 513
Size: 552 1314 3916
Fragments arranged by size:
3916 1314 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2194 3140 4685 1132
Size: 97 965 946 1545 2229
Fragments arranged by size:
2229 1545 965 946 97
PasI CC'CwG_GG
Cuts at: 1348 2116 1348
Size: 768 5014
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2024 2056 3851 3883 5364 5396 2024
Size: 32 1795 32 1481 32 2410
Fragments arranged by size:
2410 1795 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2898 2898
Size: 5782
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2703 3134 830
Size: 1873 431 3478
Fragments arranged by size:
3478 1873 431
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SapI GCTCTTCn'nnn_
Cuts at: 2087 2828 2087
Size: 741 5041
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 2534 4030 5742 2534
Size: 1496 1712 2574
Fragments arranged by size:
2574 1712 1496
SexAI A'CCwGG_T
Cuts at: 2546 2546
Size: 5782
SfcI C'TryA_G
Cuts at: 826 1045 2699 3130 4265 4946 5137 826
Size: 219 1654 431 1135 681 191 1471
Fragments arranged by size:
1654 1471 1135 681 431 219 191
SfoI GGC'GCC
Cuts at: 2665 2665
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 2691 1700
Size: 991 4791
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2379 2404 2963 3221 513
Size: 552 1314 25 559 258 3074
Fragments arranged by size:
3074 1314 559 552 258 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 3809 3826 3979 4164 4609 325
Size: 1255 590 1639 17 153 185 445 1498
Fragments arranged by size:
1639 1498 1255 590 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 4028 5740
Size: 274 80 33 51 1443 555 1496 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1712 1496 1443 555 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2355 2387 2699 2731 5742 5774 2355
Size: 32 312 32 3011 32 2363
Fragments arranged by size:
3011 2363 312 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SrfI SwaI XcmI