Restriction map for
pFN21AB8969
(Circular) (Six-base) MAPSORT of: pFN21AB8969.seq Check: 1129 from: 1 to: 5782
pFN21AB8969 5782 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3845 5229 5497 1632
Size: 2213 1384 268 1917
Fragments arranged by size:
2213 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2939 820
Size: 197 1922 3663
Fragments arranged by size:
3663 1922 197
AflIII A'CryG_T
Cuts at: 1203 2455 4681 5658 1203
Size: 1252 2226 977 1327
Fragments arranged by size:
2226 1327 1252 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2758 3525 5097 2758
Size: 767 1572 3443
Fragments arranged by size:
3443 1572 767
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2538 2696 3022 3091 3317 3469 5578 1493
Size: 1045 158 326 69 226 152 2109 1697
Fragments arranged by size:
2109 1697 1045 326 226 158 152 69
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
BamHI G'GATC_C
Cuts at: 2298 3112 3479 2298
Size: 814 367 4601
Fragments arranged by size:
4601 814 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3103 4558 618
Size: 325 217 627 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2634 2795 3101 1391
Size: 1243 161 306 4072
Fragments arranged by size:
4072 1243 306 161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2583 928
Size: 971 684 4127
Fragments arranged by size:
4127 971 684
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2726 5777 2726
Size: 3051 2731
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2369 3843 4999 1790
Size: 579 1474 1156 2573
Fragments arranged by size:
2573 1474 1156 579
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2599 3513 4471
Size: 416 53 83 399 24 1182 914 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1182 958 914 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4442 1239
Size: 3203 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2546 3589 3971 4606
Size: 53 83 186 936 1013 1043 382 635
Cuts at: 4606 275
Size: 1451
Fragments arranged by size:
1451 1043 1013 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2967 2997 1178
Size: 30 165 30 1564 30 3963
Fragments arranged by size:
3963 1564 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5782
BseYI C'CCAG_C
Cuts at: 4985 5481 4985
Size: 496 5286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3843 3928 4999 3843
Size: 85 1071 4626
Fragments arranged by size:
4626 1071 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 2634 2795 3101 3843 3928 4999
Size: 399 579 265 161 306 742 85 1071
Cuts at: 4999 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 579 399 306 265 161
85
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 2112 2157 3671 5401 2112
Size: 45 1514 1730 2493
Fragments arranged by size:
2493 1730 1514 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2075 3137 844
Size: 1231 1062 3489
Fragments arranged by size:
3489 1231 1062
BsrBI CCG'CTC
Cuts at: 1394 1823 2401 3669 1394
Size: 429 578 1268 3507
Fragments arranged by size:
3507 1268 578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2668 4277 4451 65
Size: 1183 1420 1609 174 1396
Fragments arranged by size:
1609 1420 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 4426 4613 1700
Size: 110 272 2344 187 2869
Fragments arranged by size:
2869 2344 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2221 2221
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2298 2726 2902 3112 3479 3864 3881 5333
Size: 326 428 176 210 367 385 17 1452
Cuts at: 5333 5419 5431 5777 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 428 385 367 346 326 210
176 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 487
Size: 683 56 905 4138
Fragments arranged by size:
4138 905 683 56
BtsI GCAGTG_nn'
Cuts at: 2389 3298 4092 4112 2389
Size: 909 794 20 4059
Fragments arranged by size:
4059 909 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 2701 3086 2701
Size: 385 5397
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2242 2391
Size: 54 1425 50 275 18 381 31 149
Cuts at: 2391 2811 3229 4118 5747 8
Size: 420 418 889 1629 43
Fragments arranged by size:
1629 1425 889 420 418 381 275 149
54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3712 1353
Size: 2359 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3845 4442 5229 5497 1239
Size: 393 2213 597 787 268 1524
Fragments arranged by size:
2213 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2553 2553
Size: 5782
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 4929 1678
Size: 3251 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2967 2999 4423 4455 4497
Size: 32 135 32 1395 32 1424 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1424 1395 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2954 748
Size: 2206 3576
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2768 4896 5080 1305
Size: 1463 2128 184 2007
Fragments arranged by size:
2128 2007 1463 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 2244 2393 2813 10
Size: 54 1425 343 381 31 149 420 2979
Fragments arranged by size:
2979 1425 420 381 343 149 54 31
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2117 2156 2656 3740 4099 4258
Size: 648 843 108 39 500 1084 359 159
Cuts at: 4258 518
Size: 2042
Fragments arranged by size:
2042 1084 843 648 500 359 159 108
39
MspA1I CmG'CkG
Cuts at: 1606 2045 3198 3875 5023 5268 1606
Size: 439 1153 677 1148 245 2120
Fragments arranged by size:
2120 1153 1148 677 439 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2459 2984 3140 4685 1132
Size: 97 1230 525 156 1545 2229
Fragments arranged by size:
2229 1545 1230 525 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 2455 4681 2455
Size: 2226 3556
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2758 1108
Size: 1650 4132
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2553 2553
Size: 5782
PshAI GACnn'nnGTC
Cuts at: 1613 2185 1613
Size: 572 5210
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2044 3134 830
Size: 1214 1090 3478
Fragments arranged by size:
3478 1214 1090
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2045 2045
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SexAI A'CCwGG_T
Cuts at: 2555 2555
Size: 5782
SfcI C'TryA_G
Cuts at: 826 1045 2040 3130 4265 4946 5137 826
Size: 219 995 1090 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 995 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2939 3886 4787 5049 820
Size: 197 926 996 947 901 262 1553
Fragments arranged by size:
1553 996 947 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 2427 3706 5
Size: 47 2375 1279 2081
Fragments arranged by size:
2375 2081 1279 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2190 2518 2641 2906 3221 513
Size: 552 1125 328 123 265 315 3074
Fragments arranged by size:
3074 1125 552 328 315 265 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3809 3826 3979 4164 4609 325
Size: 1255 2229 17 153 185 445 1498
Fragments arranged by size:
2229 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4028 5740 96
Size: 274 80 33 51 1443 2051 1712 138
Fragments arranged by size:
2051 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2252 2252
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 2836 3911 2836
Size: 1075 4707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI