Restriction map for
pFN21AB8967
(Circular) (Six-base) MAPSORT of: pFN21AB8967.seq Check: 2716 from: 1 to: 5773
pFN21AB8967 5773 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 2424 3094 2424
Size: 670 5103
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 3836 5220 5488 1632
Size: 425 1779 1384 268 1917
Fragments arranged by size:
1917 1779 1384 425 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 2251 4672 5649 1203
Size: 1048 2421 977 1327
Fragments arranged by size:
2421 1327 1048 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 3068 1166
Size: 1902 3871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 3516 5088 3516
Size: 1572 4201
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 2352 3082 3308 3460 5569 1493
Size: 859 730 226 152 2109 1697
Fragments arranged by size:
2109 1697 859 730 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 3098 1870
Size: 73 1155 4545
Fragments arranged by size:
4545 1155 73
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2161 2424 2473 2794
Size: 325 217 627 272 102 263 49 321
Cuts at: 2794 3094 4549 618
Size: 300 1455 1842
Fragments arranged by size:
1842 1455 627 325 321 300 272 263
217 102 49
BanII G_rGCy'C
Cuts at: 1391 2326 3092 1391
Size: 935 766 4072
Fragments arranged by size:
4072 935 766
BbeI G_GCGC'C
Cuts at: 2798 2798
Size: 5773
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2571 2770 928
Size: 971 672 199 3931
Fragments arranged by size:
3931 971 672 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2522 4384 136
Size: 107 122 71 631 605 850 1862 1525
Fragments arranged by size:
1862 1525 850 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2478 3834 4990 1790
Size: 688 1356 1156 2573
Fragments arranged by size:
2573 1356 1156 688
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 4433 1239
Size: 881 2313 2579
Fragments arranged by size:
2579 2313 881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4763 5061 3898
Size: 865 298 4610
Fragments arranged by size:
4610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 882
Size: 617 2937 2219
Fragments arranged by size:
2937 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2542 3564 493
Size: 711 1338 1022 2702
Fragments arranged by size:
2702 1338 1022 711
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 2795 3580 3962
Size: 53 83 186 936 603 659 785 382
Cuts at: 3962 4597 275
Size: 635 1451
Fragments arranged by size:
1451 936 785 659 635 603 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4206 4604 4878 5025 5436 4206
Size: 398 274 147 411 4543
Fragments arranged by size:
4543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2723 2753 1178
Size: 30 165 30 1320 30 4198
Fragments arranged by size:
4198 1320 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2482 1186
Size: 1296 4477
BseYI C'CCAG_C
Cuts at: 2154 2330 4976 5472 2154
Size: 176 2646 496 2455
Fragments arranged by size:
2646 2455 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3012 1163
Size: 573 1276 3924
Fragments arranged by size:
3924 1276 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3834 3919 4990 3834
Size: 85 1071 4617
Fragments arranged by size:
4617 1071 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2326 2478 3092 3834 3919 4990 1391
Size: 399 536 152 614 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 536 399 152 85
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3128 844
Size: 2284 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 1394
Size: 429 1837 3507
Fragments arranged by size:
3507 1837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2126 4268 4442 65
Size: 1183 878 2142 174 1396
Fragments arranged by size:
2142 1396 1183 878 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4417 4604 1700
Size: 110 2607 187 2869
Fragments arranged by size:
2869 2607 187 110
BsrGI T'GTAC_A
Cuts at: 96 2382 96
Size: 2286 3487
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5773
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstXI CCAn_nnnn'nTGG
Cuts at: 3070 3070
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768
Size: 1131 367 385 17 1452 86 12 346
Cuts at: 5768 1972
Size: 1977
Fragments arranged by size:
1977 1452 1131 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 2887 487
Size: 683 56 895 766 3373
Fragments arranged by size:
3373 895 766 683 56
BtsI GCAGTG_nn'
Cuts at: 2914 3289 4083 4103 2914
Size: 375 794 20 4584
Fragments arranged by size:
4584 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 2646 3077 2646
Size: 431 5342
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2523 3220
Size: 54 1425 50 275 18 213 480 697
Cuts at: 3220 4109 5738 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 697 480 275 213 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3703 1353
Size: 2350 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4360 4734 4880 387
Size: 1064 138 2771 374 146 1280
Fragments arranged by size:
2771 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2120 3836 4433 5220 5488 1239
Size: 393 425 63 1716 597 787 268 1524
Fragments arranged by size:
1716 1524 787 597 425 393 268 63
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 5773
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 2798 4920 1678
Size: 1120 2122 2531
Fragments arranged by size:
2531 2122 1120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4414 4446 4488 4520 1373
Size: 32 135 32 2842 32 42 32 2626
Fragments arranged by size:
2842 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2753 748
Size: 2005 3768
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KasI G'GCGC_C
Cuts at: 2794 2794
Size: 5773
KpnI G_GTAC'C
Cuts at: 2428 3098 2428
Size: 670 5103
MfeI C'AATT_G
Cuts at: 2401 3372 2401
Size: 971 4802
MluI A'CGCG_T
Cuts at: 2251 5649 2251
Size: 3398 2375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4887 5071 1305
Size: 3582 184 2007
Fragments arranged by size:
3582 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 2525 10
Size: 54 1425 343 213 480 3258
Fragments arranged by size:
3258 1425 480 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3068 3731 4090 4249 518
Size: 648 1902 663 359 159 2042
Fragments arranged by size:
2042 1902 663 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2612 2979 3189 3866 5014 5259 1606
Size: 552 454 367 210 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 454 367 245 210
NarI GG'CG_CC
Cuts at: 2795 2795
Size: 5773
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 3131 4676 1132
Size: 97 1902 1545 2229
Fragments arranged by size:
2229 1902 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3015 1348
Size: 1667 4106
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5773
PfoI T'CCnGG_A
Cuts at: 2199 2199
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 2550 3343 2550
Size: 793 4980
PstI C_TGCA'G
Cuts at: 830 3125 830
Size: 2295 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2158 2612 2158
Size: 454 5319
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 3121 4256 4937 5128 826
Size: 219 2076 1135 681 191 1471
Fragments arranged by size:
2076 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2796 2796
Size: 5773
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 3100 1872
Size: 1228 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3877 4778 5040 820
Size: 197 926 1934 901 262 1553
Fragments arranged by size:
1934 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SspI AAT'ATT
Cuts at: 5 52 2806 3697 5
Size: 47 2754 891 2081
Fragments arranged by size:
2754 2081 891 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 3212 513
Size: 552 2147 3074
Fragments arranged by size:
3074 2147 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2927 3800 3817 3970 4155 4600 325
Size: 1255 1347 873 17 153 185 445 1498
Fragments arranged by size:
1498 1347 1255 873 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 2382 4019
Size: 274 80 33 51 1443 390 15 1637
Cuts at: 4019 5731 96
Size: 1712 138
Fragments arranged by size:
1712 1637 1443 390 274 138 80 51
33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5733 5765 5733
Size: 32 5741
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2053
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 3098 1870
Size: 1228 4545
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI