Restriction map for
pFN21AB8966
(Circular) (Six-base) MAPSORT of: pFN21AB8966.seq Check: 321 from: 1 to: 5773
pFN21AB8966 5773 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2886 3583 278
Size: 53 83 186 936 1350 697 2468
Fragments arranged by size:
2468 1350 936 697 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2123 3116 1937
Size: 186 993 4594
Fragments arranged by size:
4594 993 186
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 3836 5220 5488 1632
Size: 545 1659 1384 268 1917
Fragments arranged by size:
1917 1659 1384 545 268
AflII C'TTAA_G
Cuts at: 820 1017 2770 820
Size: 197 1753 3823
Fragments arranged by size:
3823 1753 197
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 2470 3516 5088 2470
Size: 1046 1572 3155
Fragments arranged by size:
3155 1572 1046
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 2008 2015 2486 3082 3308 3460 5569 1493
Size: 515 7 471 596 226 152 2109 1697
Fragments arranged by size:
2109 1697 596 515 471 226 152 7
AseI AT'TA_AT
Cuts at: 160 1032 2129 4327 160
Size: 872 1097 2198 1606
Fragments arranged by size:
2198 1606 1097 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 2647 2698 2864 2890 3098 1870
Size: 73 339 365 51 166 26 208 4545
Fragments arranged by size:
4545 365 339 208 166 73 51 26
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 2758 2895 3094 4549
Size: 325 217 627 393 578 137 199 1455
Cuts at: 4549 618
Size: 1842
Fragments arranged by size:
1842 1455 627 578 393 325 217 199
137
BanII G_rGCy'C
Cuts at: 1391 2656 2865 3092 1391
Size: 1265 209 227 4072
Fragments arranged by size:
4072 1265 227 209
BbeI G_GCGC'C
Cuts at: 2184 2899 2184
Size: 715 5058
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2878 2886 928
Size: 971 979 8 3815
Fragments arranged by size:
3815 979 971 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2802 2836 3964 3998 1903
Size: 34 865 34 1128 34 3678
Fragments arranged by size:
3678 1128 865 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2276 2369 2703
Size: 107 122 71 631 605 604 93 334
Cuts at: 2703 2720 4384 136
Size: 17 1664 1525
Fragments arranged by size:
1664 1525 631 605 604 334 122 107
93 71 17
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 3834 4990 1790
Size: 2044 1156 2573
Fragments arranged by size:
2573 2044 1156
BmgBI CAC'GTC
Cuts at: 1318 2340 1318
Size: 1022 4751
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2498 2678 2879 2936 4433 1239
Size: 917 342 180 201 57 1497 2579
Fragments arranged by size:
2579 1497 917 342 201 180 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4763 5061 3898
Size: 865 298 4610
Fragments arranged by size:
4610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2191 4436 882
Size: 617 692 2245 2219
Fragments arranged by size:
2245 2219 692 617
BsaAI yAC'GTr
Cuts at: 493 1204 3564 493
Size: 711 2360 2702
Fragments arranged by size:
2702 2360 711
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 2883 2896 3580
Size: 53 83 186 936 648 702 13 684
Cuts at: 3580 3962 4597 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 702 684 648 635 382 186
83 53 13
BsaWI w'CCGG_w
Cuts at: 2960 4206 4604 4878 5025 5436 2960
Size: 1246 398 274 147 411 3297
Fragments arranged by size:
3297 1246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 1178
Size: 30 165 30 615 30 4903
Fragments arranged by size:
4903 615 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2130 2133 1186
Size: 941 3 3 4826
Fragments arranged by size:
4826 941 3 3
BseYI C'CCAG_C
Cuts at: 2165 2324 4976 5472 2165
Size: 159 2652 496 2466
Fragments arranged by size:
2652 2466 496 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5200
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2355 2940 3223 3984 5012 1540
Size: 275 186 354 585 283 761 1028 2301
Fragments arranged by size:
2301 1028 761 585 354 283 275 186
BsiHKAI G_wGCw'C
Cuts at: 3834 3919 4990 3834
Size: 85 1071 4617
Fragments arranged by size:
4617 1071 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
BsmBI CGTCTCn'nnnn_
Cuts at: 2346 2346
Size: 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2656 2865 3092 3834 3919 4990 1391
Size: 399 866 209 227 742 85 1071 2174
Fragments arranged by size:
2174 1071 866 742 399 227 209 85
BspEI T'CCGG_A
Cuts at: 2960 5436 2960
Size: 2476 3297
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2551 3069 3128 844
Size: 1707 518 59 3489
Fragments arranged by size:
3489 1707 518 59
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 1394
Size: 429 1837 3507
Fragments arranged by size:
3507 1837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2870 4268 4442 65
Size: 1183 1622 1398 174 1396
Fragments arranged by size:
1622 1398 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2511 2583 2849 4417 4604 1700
Size: 110 701 72 266 1568 187 2869
Fragments arranged by size:
2869 1568 701 266 187 110 72
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 2432 2835 1925
Size: 507 403 4863
Fragments arranged by size:
4863 507 403
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5773
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768
Size: 1131 367 385 17 1452 86 12 346
Cuts at: 5768 1972
Size: 1977
Fragments arranged by size:
1977 1452 1131 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2688 513
Size: 552 1623 3598
Fragments arranged by size:
3598 1623 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 3289 4083 4103 3289
Size: 794 20 4959
Fragments arranged by size:
4959 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3002 4780 809
Size: 507 1686 1778 1802
Fragments arranged by size:
1802 1778 1686 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 2513 2691
Size: 54 1425 50 275 18 522 161 178
Cuts at: 2691 2937 3220 4109 5738 8
Size: 246 283 889 1629 43
Fragments arranged by size:
1629 1425 889 522 283 275 246 178
161 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2352 2937 3220 1537
Size: 275 540 585 283 4090
Fragments arranged by size:
4090 585 540 283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3015 3703 1353
Size: 1662 688 3423
Fragments arranged by size:
3423 1662 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2302 2323 2350 2701 4360 4734
Size: 1064 138 713 21 27 351 1659 374
Cuts at: 4734 4880 387
Size: 146 1280
Fragments arranged by size:
1659 1280 1064 713 374 351 146 138
27 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2177 2498 2678 2879 2936 3836
Size: 393 524 21 321 180 201 57 900
Cuts at: 3836 4433 5220 5488 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 900 787 597 524 393 321 268
201 180 57 21
EcoO109I rG'GnC_Cy
Cuts at: 2766 2766
Size: 5773
EcoRI G'AATT_C
Cuts at: 2486 3082 3460 2486
Size: 596 378 4799
Fragments arranged by size:
4799 596 378
FspI TGC'GCA
Cuts at: 2444 4279 2444
Size: 1835 3938
HaeII r_GCGC'y
Cuts at: 1678 2184 2899 4920 1678
Size: 506 715 2021 2531
Fragments arranged by size:
2531 2021 715 506
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 2873 2905 4414
Size: 32 135 32 722 32 547 32 1509
Cuts at: 4414 4446 4488 4520 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1509 722 547 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2124 3117 3363 669
Size: 988 281 186 993 246 3079
Fragments arranged by size:
3079 993 988 281 246 186
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KasI G'GCGC_C
Cuts at: 2180 2895 2180
Size: 715 5058
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2362 4887 5071 1305
Size: 1057 2525 184 2007
Fragments arranged by size:
2525 2007 1057 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3951
Fragments arranged by size:
3951 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3731 4090 4249 518
Size: 648 2565 359 159 2042
Fragments arranged by size:
2565 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2267 2467 2690 3189 3866 5014 5259
Size: 478 183 200 223 499 677 1148 245
Cuts at: 5259 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 499 478 245 223 200
183
NaeI GCC'GGC
Cuts at: 2513 2851 2513
Size: 338 5435
NarI GG'CG_CC
Cuts at: 2181 2896 2181
Size: 715 5058
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NgoMIV G'CCGG_C
Cuts at: 2511 2849 2511
Size: 338 5435
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 3131 4676 1132
Size: 97 1902 1545 2229
Fragments arranged by size:
2229 1902 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2946 1613
Size: 1333 4440
PsiI TTA'TAA
Cuts at: 2039 3343 2039
Size: 1304 4469
PstI C_TGCA'G
Cuts at: 830 2395 3066 3125 830
Size: 1565 671 59 3478
Fragments arranged by size:
3478 1565 671 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2467 2467
Size: 5773
SacII CC_GC'GG
Cuts at: 2691 2691
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 2122 3115 1936
Size: 186 993 4594
Fragments arranged by size:
4594 993 186
SapI GCTCTTCn'nnn_
Cuts at: 3015 3015
Size: 5773
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 2391 2981 3062 3121 4256 4937 5128
Size: 219 1346 590 81 59 1135 681 191
Cuts at: 5128 826
Size: 1471
Fragments arranged by size:
1471 1346 1135 681 590 219 191 81
59
SfoI GGC'GCC
Cuts at: 2182 2897 2182
Size: 715 5058
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 2649 2700 2866 3100 1872
Size: 777 51 166 234 4545
Fragments arranged by size:
4545 777 234 166 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2770 3877 4778 5040 820
Size: 197 926 827 1107 901 262 1553
Fragments arranged by size:
1553 1107 926 901 827 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SrfI GCCC'GGGC
Cuts at: 2700 2866 2700
Size: 166 5607
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 2967 1517
Size: 1450 4323
StyI C'CwwG_G
Cuts at: 513 1065 2291 3212 513
Size: 552 1226 921 3074
Fragments arranged by size:
3074 1226 921 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3800 3817 3970 4155 4600 325
Size: 1255 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4019 5731 96
Size: 274 80 33 51 1443 2042 1712 138
Fragments arranged by size:
2042 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5733 5765 5733
Size: 32 5741
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 2647 2698 2864 3098 1870
Size: 777 51 166 234 4545
Fragments arranged by size:
4545 777 234 166 51
XmnI GAAnn'nnTTC
Cuts at: 2486 3902 2486
Size: 1416 4357
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2884 3581 276
Size: 53 83 186 936 1350 697 2468
Fragments arranged by size:
2468 1350 936 697 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SwaI XcmI