Restriction map for
pFN21AB8961
(Circular) (Six-base) MAPSORT of: pFN21AB8961.seq Check: 2497 from: 1 to: 5731
pFN21AB8961 5731 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2114 2114
Size: 5731
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2759 3794 5178 5446 1632
Size: 1127 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1127 1035 268
AfeI AGC'GCT
Cuts at: 2840 2840
Size: 5731
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 2895 4630 5607 1203
Size: 1692 1735 977 1327
Fragments arranged by size:
1735 1692 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 3474 5046 3474
Size: 1572 4159
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 2882 3040 3266 3418 5527 1493
Size: 1389 158 226 152 2109 1697
Fragments arranged by size:
2109 1697 1389 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2353 2466 2562 2588 2907 3056 1870
Size: 73 410 113 96 26 319 149 4545
Fragments arranged by size:
4545 410 319 149 113 96 73 26
BamHI G'GATC_C
Cuts at: 2811 3061 3428 2811
Size: 250 367 5114
Fragments arranged by size:
5114 367 250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2218 2439 3052 4507
Size: 325 217 627 231 200 221 613 1455
Cuts at: 4507 618
Size: 1842
Fragments arranged by size:
1842 1455 627 613 325 231 221 217
200
BanII G_rGCy'C
Cuts at: 1391 2310 2700 3050 1391
Size: 919 390 350 4072
Fragments arranged by size:
4072 919 390 350
BbeI G_GCGC'C
Cuts at: 2443 2443
Size: 5731
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 928
Size: 971 102 4658
Fragments arranged by size:
4658 971 102
BbvCI CC'TCA_GC
Cuts at: 2024 2701 2024
Size: 677 5054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2061 2098 2424
Size: 107 122 71 631 605 389 37 326
Cuts at: 2424 4342 136
Size: 1918 1525
Fragments arranged by size:
1918 1525 631 605 389 326 122 107
71 37
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 2223 3792 4948 1790
Size: 433 1569 1156 2573
Fragments arranged by size:
2573 1569 1156 433
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2685 3462 4420
Size: 416 53 83 399 24 1268 777 958
Cuts at: 4420 442
Size: 1753
Fragments arranged by size:
1753 1268 958 777 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2894 4391 1239
Size: 1655 1497 2579
Fragments arranged by size:
2579 1655 1497
Bpu10I CC'TnA_GC
Cuts at: 2024 2701 2024
Size: 677 5054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2888 3856 4721 5019 2888
Size: 968 865 298 3600
Fragments arranged by size:
3600 968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2038 2320 3522 493
Size: 711 834 282 1202 2702
Fragments arranged by size:
2702 1202 834 711 282
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2440 3538 3920 4555
Size: 53 83 186 936 907 1098 382 635
Cuts at: 4555 275
Size: 1451
Fragments arranged by size:
1451 1098 936 907 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 2807 2837 1178
Size: 30 165 30 906 30 468 30 4072
Fragments arranged by size:
4072 906 468 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2497 2500 2503 2521 2524 2759 2762 1186
Size: 1311 3 3 18 3 235 3 4155
Fragments arranged by size:
4155 1311 235 18 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2226 2408 2420 2428 4934 5430 2226
Size: 182 12 8 2506 496 2527
Fragments arranged by size:
2527 2506 496 182 12 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2145 2193 2314 3181 3942 4970
Size: 275 186 144 48 121 867 761 1028
Cuts at: 4970 1540
Size: 2301
Fragments arranged by size:
2301 1028 867 761 275 186 144 121
48
BsiHKAI G_wGCw'C
Cuts at: 2555 3792 3877 4948 2555
Size: 1237 85 1071 3338
Fragments arranged by size:
3338 1237 1071 85
BsiWI C'GTAC_G
Cuts at: 2268 2268
Size: 5731
BsmI GAATG_Cn'
Cuts at: 2758 3242 3335 2758
Size: 484 93 5154
Fragments arranged by size:
5154 484 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3021 3021
Size: 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2310 2555 2700 3050 3792 3877
Size: 399 433 87 245 145 350 742 85
Cuts at: 3877 4948 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 350 245 145
87 85
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2114 3086 844
Size: 1270 972 3489
Fragments arranged by size:
3489 1270 972
BsrBI CCG'CTC
Cuts at: 1394 1823 2253 2471 3618 1394
Size: 429 430 218 1147 3507
Fragments arranged by size:
3507 1147 430 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2115 2913 4375 4562 1700
Size: 110 305 798 1462 187 2869
Fragments arranged by size:
2869 1462 798 305 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 2081 2106 1925
Size: 156 25 5550
Fragments arranged by size:
5550 156 25
BssSI C'ACGA_G
Cuts at: 2167 2206 2596 3791 4803 2167
Size: 39 390 1195 1012 3095
Fragments arranged by size:
3095 1195 1012 390 39
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 2811 3061 3428 3813 3830 5282 5368 5380
Size: 839 250 367 385 17 1452 86 12
Cuts at: 5380 5726 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 839 385 367 346 250 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2214 2401 2487 2556 513
Size: 552 1149 187 86 69 3688
Fragments arranged by size:
3688 1149 552 187 86 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2261 487
Size: 683 56 1035 3957
Fragments arranged by size:
3957 1035 683 56
BtsI GCAGTG_nn'
Cuts at: 3247 4041 4061 3247
Size: 794 20 4917
Fragments arranged by size:
4917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2190 2289
Size: 54 1425 50 275 18 312 48 99
Cuts at: 2289 2311 2928 3178 4067 5696 8
Size: 22 617 250 889 1629 43
Fragments arranged by size:
1629 1425 889 617 312 275 250 99
54 50 48 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2142 2190 2311 3178 1537
Size: 275 330 48 121 867 4090
Fragments arranged by size:
4090 867 330 275 121 48
EarI CTCTTCn'nnn_
Cuts at: 1353 2894 3661 1353
Size: 1541 767 3423
Fragments arranged by size:
3423 1541 767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2759 2894 3794 4391 5178 5446 1239
Size: 393 1127 135 900 597 787 268 1524
Fragments arranged by size:
1524 1127 900 787 597 393 268 135
EcoNI CCTnn'n_nnAGG
Cuts at: 2655 2655
Size: 5731
EcoRI G'AATT_C
Cuts at: 2882 3040 3418 2882
Size: 158 378 5195
Fragments arranged by size:
5195 378 158
FseI GG_CCGG'CC
Cuts at: 2119 2119
Size: 5731
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 2443 2842 2920 4878 1678
Size: 765 399 78 1958 2531
Fragments arranged by size:
2531 1958 765 399 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2803 2835 4372 4404 4446
Size: 32 135 32 1231 32 1537 32 42
Cuts at: 4446 4478 1373
Size: 32 2626
Fragments arranged by size:
2626 1537 1231 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KasI G'GCGC_C
Cuts at: 2439 2439
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2673 4845 5029 1305
Size: 1368 2172 184 2007
Fragments arranged by size:
2172 2007 1368 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2930 10
Size: 54 1425 343 1098 2811
Fragments arranged by size:
2811 1425 1098 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 4048 4207 518
Size: 648 2523 359 159 2042
Fragments arranged by size:
2523 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 2240 2341 2403 2424 2583 2630 3147
Size: 610 24 101 62 21 159 47 517
Cuts at: 3147 3824 4972 5217 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 610 517 245 159 101
62 47 24 21
NaeI GCC'GGC
Cuts at: 2117 2117
Size: 5731
NarI GG'CG_CC
Cuts at: 2440 2440
Size: 5731
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5179
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NgoMIV G'CCGG_C
Cuts at: 2115 2115
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2899 3089 4634 1132
Size: 97 1670 190 1545 2229
Fragments arranged by size:
2229 1670 1545 190 97
PasI CC'CwG_GG
Cuts at: 1348 2572 1348
Size: 1224 4507
PciI A'CATG_T
Cuts at: 2895 4630 2895
Size: 1735 3996
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PfoI T'CCnGG_A
Cuts at: 2327 2327
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2125 2616 3083 830
Size: 1295 491 467 3478
Fragments arranged by size:
3478 1295 491 467
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
RsrII CG'GwC_CG
Cuts at: 2393 2393
Size: 5731
SacII CC_GC'GG
Cuts at: 2217 2404 2217
Size: 187 5544
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 2125 2616 3083 2125
Size: 491 467 4773
Fragments arranged by size:
4773 491 467
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2121 2612 3079 4214 4895 5086 826
Size: 219 1076 491 467 1135 681 191 1471
Fragments arranged by size:
1471 1135 1076 681 491 467 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2098 2424 2098
Size: 326 5405
SfoI GGC'GCC
Cuts at: 2441 2441
Size: 5731
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2355 2564 2909 3058 1872
Size: 483 209 345 149 4545
Fragments arranged by size:
4545 483 345 209 149
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2867 3835 4736 4998 820
Size: 197 926 924 968 901 262 1553
Fragments arranged by size:
1553 968 926 924 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 3170 513
Size: 552 2105 3074
Fragments arranged by size:
3074 2105 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3758 3775 3928 4113 4558 325
Size: 1255 2178 17 153 185 445 1498
Fragments arranged by size:
2178 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3977 5689 96
Size: 274 80 33 51 1443 2000 1712 138
Fragments arranged by size:
2000 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2807 2839 5691 5723 2807
Size: 32 2852 32 2815
Fragments arranged by size:
2852 2815 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 2353 2562 2907 3056 1870
Size: 483 209 345 149 4545
Fragments arranged by size:
4545 483 345 209 149
XmnI GAAnn'nnTTC
Cuts at: 2882 3860 2882
Size: 978 4753
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI RsrII SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FspAI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
SacI SanDI SapI SexAI SrfI SwaI XcmI