Restriction map for
pFN21AB8960
(Circular) (Six-base) MAPSORT of: pFN21AB8960.seq Check: 8115 from: 1 to: 5725
pFN21AB8960 5725 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2238 3068 1937
Size: 301 830 4594
Fragments arranged by size:
4594 830 301
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3788 5172 5440 1632
Size: 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2126 1184
Size: 942 4783
AlwNI CAG_nnn'CTG
Cuts at: 2067 2404 3468 5040 2067
Size: 337 1064 1572 2752
Fragments arranged by size:
2752 1572 1064 337
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2660 3050 1870
Size: 73 717 390 4545
Fragments arranged by size:
4545 717 390 73
BamHI G'GATC_C
Cuts at: 2991 3055 3422 2991
Size: 64 367 5294
Fragments arranged by size:
5294 367 64
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2814 3046 4501 618
Size: 325 217 627 1027 232 1455 1842
Fragments arranged by size:
1842 1455 1027 627 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2616 2634 2660 3044 1391
Size: 1225 18 26 384 4072
Fragments arranged by size:
4072 1225 384 26 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4754
BbvCI CC'TCA_GC
Cuts at: 2703 2703
Size: 5725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 2058 1548
Size: 510 5215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2517 4336 136
Size: 107 122 71 631 605 845 1819 1525
Fragments arranged by size:
1819 1525 845 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2261 5720 2261
Size: 3459 2266
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2819 3786 4942 1790
Size: 1029 967 1156 2573
Fragments arranged by size:
2573 1156 1029 967
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 4414 442
Size: 416 53 83 399 24 2039 958 1753
Fragments arranged by size:
2039 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 4385 1239
Size: 1283 1863 2579
Fragments arranged by size:
2579 1863 1283
Bpu10I CC'TnA_GC
Cuts at: 2703 2703
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2217 2951 3850 4715 5013 2217
Size: 734 899 865 298 2929
Fragments arranged by size:
2929 899 865 734 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2713 4388 882
Size: 617 1214 1675 2219
Fragments arranged by size:
2219 1675 1214 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3532 3914 4549 275
Size: 53 83 186 936 1999 382 635 1451
Fragments arranged by size:
1999 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2988 4158 4556 4830 4977 5388 2988
Size: 1170 398 274 147 411 3325
Fragments arranged by size:
3325 1170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 1178
Size: 30 165 30 662 30 4808
Fragments arranged by size:
4808 662 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2704 1186
Size: 1518 4207
BseYI C'CCAG_C
Cuts at: 2583 4928 5424 2583
Size: 2345 496 2884
Fragments arranged by size:
2884 2345 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2750 1163
Size: 573 1014 4138
Fragments arranged by size:
4138 1014 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2382 3175 3936 4964 1540
Size: 275 186 381 793 761 1028 2301
Fragments arranged by size:
2301 1028 793 761 381 275 186
BsiHKAI G_wGCw'C
Cuts at: 3786 3871 4942 3786
Size: 85 1071 4569
Fragments arranged by size:
4569 1071 85
BsmI GAATG_Cn'
Cuts at: 2151 3236 3329 2151
Size: 1085 93 4547
Fragments arranged by size:
4547 1085 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2175 2175
Size: 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2616 2634 2660 2819 3044 3786 3871
Size: 399 826 18 26 159 225 742 85
Cuts at: 3871 4942 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 826 742 399 225 159 85
26 18
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2920 3080 844
Size: 2076 160 3489
Fragments arranged by size:
3489 2076 160
BsrBI CCG'CTC
Cuts at: 1394 1823 2622 3612 1394
Size: 429 799 990 3507
Fragments arranged by size:
3507 990 799 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 2106 96
Size: 2010 3715
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 2550 3785 4797 2550
Size: 1235 1012 3478
Fragments arranged by size:
3478 1235 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2251 2261 2991 3055 3422 3807 3824 5276
Size: 279 10 730 64 367 385 17 1452
Cuts at: 5276 5362 5374 5720 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 730 385 367 346 279 86
64 17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2436 2436
Size: 5725
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2787 3241 4035 4055 2787
Size: 454 794 20 4457
Fragments arranged by size:
4457 794 454 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2533 2568 529
Size: 35 1969 35 3686
Fragments arranged by size:
3686 1969 35 35
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2930 4732 809
Size: 507 1614 1802 1802
Fragments arranged by size:
1802 1802 1614 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2379 2559
Size: 54 1425 50 275 18 345 204 180
Cuts at: 2559 2623 3172 4061 5690 8
Size: 64 549 889 1629 43
Fragments arranged by size:
1629 1425 889 549 345 275 204 180
64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2379 3172 1537
Size: 275 567 793 4090
Fragments arranged by size:
4090 793 567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2679 2804 3655 1353
Size: 1326 125 851 3423
Fragments arranged by size:
3423 1326 851 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 3788 4385 5172 5440 1239
Size: 393 890 1266 597 787 268 1524
Fragments arranged by size:
1524 1266 890 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2702 2702
Size: 5725
EcoO109I rG'GnC_Cy
Cuts at: 2136 2386 2136
Size: 250 5475
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2693 2693
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2100 2890 4872 1678
Size: 422 790 1982 2531
Fragments arranged by size:
2531 1982 790 422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373
Size: 32 135 32 2794 32 42 32 2626
Fragments arranged by size:
2794 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2914 3069 3315 669
Size: 988 281 976 155 246 3079
Fragments arranged by size:
3079 988 976 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 2914 3315 2914
Size: 401 5324
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 2338 2779 3324 2338
Size: 441 545 4739
Fragments arranged by size:
4739 545 441
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2225 4839 5023 1305
Size: 920 2614 184 2007
Fragments arranged by size:
2614 2007 920 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 10
Size: 54 1425 343 345 3558
Fragments arranged by size:
3558 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2531 3683 4042 4201 518
Size: 648 942 423 1152 359 159 2042
Fragments arranged by size:
2042 1152 942 648 423 359 159
MspA1I CmG'CkG
Cuts at: 1606 2579 2587 3141 3818 4966 5211 1606
Size: 973 8 554 677 1148 245 2120
Fragments arranged by size:
2120 1148 973 677 554 245 8
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2107 2113 2350 3083 4628 1132
Size: 97 878 6 237 733 1545 2229
Fragments arranged by size:
2229 1545 878 733 237 97 6
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 2404 2781 2839 1108
Size: 1095 201 377 58 3994
Fragments arranged by size:
3994 1095 377 201 58
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 3794 3826 5307 5339 2846
Size: 32 916 32 1481 32 3232
Fragments arranged by size:
3232 1481 916 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2136 2386 2136
Size: 250 5475
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PstI C_TGCA'G
Cuts at: 830 2072 2592 3077 830
Size: 1242 520 485 3478
Fragments arranged by size:
3478 1242 520 485
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2587 2587
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 2072 3077 2072
Size: 1005 4720
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2068 2588 3073 4208 4889 5080 826
Size: 219 1023 520 485 1135 681 191 1471
Fragments arranged by size:
1471 1135 1023 681 520 485 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2232 2966 3829 4730 4992 820
Size: 197 926 289 734 863 901 262 1553
Fragments arranged by size:
1553 926 901 863 734 289 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 3164 513
Size: 552 2099 3074
Fragments arranged by size:
3074 2099 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3752 3769 3922 4107 4552 325
Size: 1255 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2499 3971
Size: 274 80 33 51 1443 129 393 1472
Cuts at: 3971 5683 96
Size: 1712 138
Fragments arranged by size:
1712 1472 1443 393 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2280 2312 5685 5717 2280
Size: 32 3373 32 2288
Fragments arranged by size:
3373 2288 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2466 1316
Size: 1150 4575
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2778 2778
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI