Restriction map for
pFN21AB8956
(Circular) (Six-base) MAPSORT of: pFN21AB8956.seq Check: 5502 from: 1 to: 5704
pFN21AB8956 5704 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2740 2987 2740
Size: 247 5457
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2036 3514 278
Size: 53 83 186 936 500 1478 2468
Fragments arranged by size:
2468 1478 936 500 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 2231 3025 2231
Size: 794 4910
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2753 3767 5151 5419 1632
Size: 1121 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1121 1014 268
AfeI AGC'GCT
Cuts at: 2627 2690 2627
Size: 63 5641
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 2521 2523 4603 5580 1203
Size: 1318 2 2080 977 1327
Fragments arranged by size:
2080 1327 1318 977 2
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 3447 5019 3447
Size: 1572 4132
ApaI G_GGCC'C
Cuts at: 2706 2706
Size: 5704
ApaLI G'TGCA_C
Cuts at: 2517 3761 4917 2517
Size: 1244 1156 3304
Fragments arranged by size:
3304 1244 1156
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2705 3029 1870
Size: 73 168 594 324 4545
Fragments arranged by size:
4545 594 324 168 73
BamHI G'GATC_C
Cuts at: 2366 3034 3401 2366
Size: 668 367 4669
Fragments arranged by size:
4669 668 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2100 2107 2198 2231
Size: 325 217 627 231 82 7 91 33
Cuts at: 2231 2605 2824 2996 3025 4480 618
Size: 374 219 172 29 1455 1842
Fragments arranged by size:
1842 1455 627 374 325 231 219 217
172 91 82 33 29 7
BanII G_rGCy'C
Cuts at: 1391 2267 2706 3023 1391
Size: 876 439 317 4072
Fragments arranged by size:
4072 876 439 317
BbeI G_GCGC'C
Cuts at: 2022 2104 2111 2202 2609 2828 2022
Size: 82 7 91 407 219 4898
Fragments arranged by size:
4898 407 219 91 82 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2650 928
Size: 971 751 3982
Fragments arranged by size:
3982 971 751
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2098 2271 2348
Size: 107 122 71 631 605 426 173 77
Cuts at: 2348 2961 4315 136
Size: 613 1354 1525
Fragments arranged by size:
1525 1354 631 613 605 426 173 122
107 77 71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 2294 3121 2294
Size: 827 4877
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2521 2706 3765 4921 1790
Size: 330 401 185 1059 1156 2573
Fragments arranged by size:
2573 1156 1059 401 330 185
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3435 4393 442
Size: 416 53 83 399 24 2018 958 1753
Fragments arranged by size:
2018 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2687 4364 1239
Size: 1448 1677 2579
Fragments arranged by size:
2579 1677 1448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2392 3829 4694 4992 2392
Size: 1437 865 298 3104
Fragments arranged by size:
3104 1437 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2524 3495 493
Size: 711 1320 971 2702
Fragments arranged by size:
2702 1320 971 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 2033 2101 2108
Size: 53 83 186 936 486 14 68 7
Cuts at: 2108 2199 2219 2577 2606 2825 3511 3893 4528
Size: 91 20 358 29 219 686 382 635
Cuts at: 4528 275
Size: 1451
Fragments arranged by size:
1451 936 686 635 486 382 358 219
186 91 83 68 53 29 20 14
7
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 1178
Size: 30 165 30 867 30 4582
Fragments arranged by size:
4582 867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2315 2435 2600 2603 2606 2609 1186
Size: 898 231 120 165 3 3 3 4281
Fragments arranged by size:
4281 898 231 165 120 3 3 3
BseYI C'CCAG_C
Cuts at: 2274 2964 4907 5403 2274
Size: 690 1943 496 2575
Fragments arranged by size:
2575 1943 690 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2557 1163
Size: 573 821 4310
Fragments arranged by size:
4310 821 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2476 3154 3915 4943 1540
Size: 275 186 475 678 761 1028 2301
Fragments arranged by size:
2301 1028 761 678 475 275 186
BsiHKAI G_wGCw'C
Cuts at: 2521 3765 3850 4921 2521
Size: 1244 85 1071 3304
Fragments arranged by size:
3304 1244 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
BsmBI CGTCTCn'nnnn_
Cuts at: 2226 2470 2738 2226
Size: 244 268 5192
Fragments arranged by size:
5192 268 244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2267 2521 2706 3023 3765 3850
Size: 399 330 147 254 185 317 742 85
Cuts at: 3850 4921 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 330 317 254 185
147 85
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 2740 2820 2892 2987 3059 844
Size: 1800 96 80 72 95 72 3489
Fragments arranged by size:
3489 1800 96 95 80 72 72
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2228 2351 2386 2546 2569 2599 2793
Size: 110 418 123 35 160 23 30 194
Cuts at: 2793 4348 4535 1700
Size: 1555 187 2869
Fragments arranged by size:
2869 1555 418 194 187 160 123 110
35 30 23
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 2710 1925
Size: 785 4919
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5704
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2842 2842
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2366 3034 3401 3786 3803 5255 5341 5353
Size: 394 668 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 668 394 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2430 513
Size: 552 939 132 294 3787
Fragments arranged by size:
3787 939 552 294 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 2921 3220 4014 4034 2921
Size: 299 794 20 4591
Fragments arranged by size:
4591 794 299 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2133 2380
Size: 54 1425 50 275 18 255 48 247
Cuts at: 2380 2400 2721 2844 2903 3151 4040 5669 8
Size: 20 321 123 59 248 889 1629 43
Fragments arranged by size:
1629 1425 889 321 275 255 248 247
123 59 54 50 48 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3634 1353
Size: 2281 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2446 4291 4665 4811 387
Size: 1064 138 857 1845 374 146 1280
Fragments arranged by size:
1845 1280 1064 857 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2687 2753 3767 4364 5151 5419 1239
Size: 393 1055 66 1014 597 787 268 1524
Fragments arranged by size:
1524 1055 1014 787 597 393 268 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2420 2420
Size: 5704
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FseI GG_CCGG'CC
Cuts at: 2355 2355
Size: 5704
FspI TGC'GCA
Cuts at: 2983 4210 2983
Size: 1227 4477
HaeII r_GCGC'y
Cuts at: 1678 2022 2104 2111 2202 2323 2609 2629 2674
Size: 344 82 7 91 121 286 20 45
Cuts at: 2674 2692 2794 2828 2972 4851 1678
Size: 18 102 34 144 1879 2531
Fragments arranged by size:
2531 1879 344 286 144 121 102 91
82 45 34 20 18 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 4345 4377 4419
Size: 32 135 32 698 32 2043 32 42
Cuts at: 4419 4451 1373
Size: 32 2626
Fragments arranged by size:
2626 2043 698 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KasI G'GCGC_C
Cuts at: 2018 2100 2107 2198 2605 2824 2018
Size: 82 7 91 407 219 4898
Fragments arranged by size:
4898 407 219 91 82 7
KpnI G_GTAC'C
Cuts at: 2235 3029 2235
Size: 794 4910
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4818 5002 1305
Size: 3513 184 2007
Fragments arranged by size:
3513 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3882
Fragments arranged by size:
3882 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3662 4021 4180 518
Size: 648 843 1653 359 159 2042
Fragments arranged by size:
2042 1653 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2123 2138 2147 2243 2346 2432 2541 2565
Size: 517 15 9 96 103 86 109 24
Cuts at: 2565 2987 3120 3797 4945 5190 1606
Size: 422 133 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 517 422 245 133 109
103 96 86 24 15 9
NaeI GCC'GGC
Cuts at: 2353 2548 2571 2601 2795 2353
Size: 195 23 30 194 5262
Fragments arranged by size:
5262 195 194 30 23
NarI GG'CG_CC
Cuts at: 2019 2101 2108 2199 2606 2825 2019
Size: 82 7 91 407 219 4898
Fragments arranged by size:
4898 407 219 91 82 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4213
Fragments arranged by size:
4213 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NgoMIV G'CCGG_C
Cuts at: 2351 2546 2569 2599 2793 2351
Size: 195 23 30 194 5262
Fragments arranged by size:
5262 195 194 30 23
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PmlI CAC'GTG
Cuts at: 2524 2524
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PspOMI G'GGCC_C
Cuts at: 2702 2702
Size: 5704
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 5672
PstI C_TGCA'G
Cuts at: 830 2928 3056 830
Size: 2098 128 3478
Fragments arranged by size:
3478 2098 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
PvuII CAG'CTG
Cuts at: 2243 2987 2243
Size: 744 4960
SacII CC_GC'GG
Cuts at: 2139 2433 2139
Size: 294 5410
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 2924 3052 4187 4868 5059 826
Size: 219 1879 128 1135 681 191 1471
Fragments arranged by size:
1879 1471 1135 681 219 191 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 2348 2348
Size: 5704
SfoI GGC'GCC
Cuts at: 2020 2102 2109 2200 2607 2826 2020
Size: 82 7 91 407 219 4898
Fragments arranged by size:
4898 407 219 91 82 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 2113 3031 1872
Size: 241 918 4545
Fragments arranged by size:
4545 918 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2371 3808 4709 4971 820
Size: 197 926 428 1437 901 262 1553
Fragments arranged by size:
1553 1437 926 901 428 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2004 2483 3143 513
Size: 552 939 479 660 3074
Fragments arranged by size:
3074 939 660 552 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 3014 3731 3748 3901 4086 4531
Size: 1255 924 510 717 17 153 185 445
Cuts at: 4531 325
Size: 1498
Fragments arranged by size:
1498 1255 924 717 510 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3950 5662 96
Size: 274 80 33 51 1443 1973 1712 138
Fragments arranged by size:
1973 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 2111 3029 1870
Size: 241 918 4545
Fragments arranged by size:
4545 918 241
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2034 3512 276
Size: 53 83 186 936 500 1478 2468
Fragments arranged by size:
2468 1478 936 500 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI
FspAI NruI NsiI PacI PfoI PpuMI PspXI RsrII
SacI SanDI SapI SexAI SrfI SwaI XcmI